Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
521,525 |
G→T |
65.3% |
V515V (GTG→GTT) |
uvrB → |
excinulease of nucleotide excision repair, DNA damage recognition component |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 521,525 | 0 | G | T | 65.3%
| 32.3
/ 38.4
| 49 | V515V (GTG→GTT) | uvrB | excinulease of nucleotide excision repair, DNA damage recognition component |
| Reads supporting (aligned to +/- strand): ref base G (15/2); new base T (20/12); total (35/14) |
| Fisher's exact test for biased strand distribution p-value = 9.59e-02 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GATCAACTTACTGCGCGAAGGTCTGGATATGCCGGAAGTGTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGCGTTCCGAACGTTCGTTGATCCA > minE/521486‑521590
|
gATCAACTTACTGCGCGAAGGTCTGGATATGCCGGAAGTGTCGCTGGTGGCGATCCTCGACGCTGa > 1:1314997/1‑66 (MQ=255)
gATCAACTTACTGCGCGAAGGTCTGGATATGCCGGAAGTGTCGCTGGTGGCGATCCTCGACGCTGa > 1:820100/1‑66 (MQ=255)
gATCAACTTACTGCGCGAAGGTCTGGATATGCCGGAAGTGTCGCTGGTGGCGATCCTCGACGCTGa > 1:2086640/1‑66 (MQ=255)
aTCAACTTACTGCGCGAAGGTCTGGATATGCCGGAAGTGTCGCTGGTGGCGATCCTCGACGCTGACaa > 1:1129919/1‑68 (MQ=255)
aTCAACTTACTGCGCGAAGGTCTGGATATGCCGGAAGTGTCGCTGGTGGCGATCCTCGACGCTGACaa > 1:84162/1‑68 (MQ=255)
aTCAACTTACTGCGCGAAGGTCTGGATATGCCGGAAGTGTCGCTGGTGGCGATCCTCGACGCTGACaa > 1:2915933/1‑68 (MQ=255)
aTCAACTTACTGCGCGAAGGTCTGGATATGCCGGAAGTGTCGCTGGTGGCGATCCTCGACGCTGACaa > 1:3377183/1‑68 (MQ=255)
aTCAACTTACTGCGCGAAGGTCTGGATATGCCGGAAGTGTCGCTGGTGGCGATCCTCGACGCTGACaa > 1:659217/1‑68 (MQ=255)
tACTGCGCGAAGGTCTGGATATGCCGGAAGTGTCGCTTGTGGCGATCCTCGACGCTGACAAAGAAGGCt < 1:2849758/69‑1 (MQ=255)
tACTGCGCGAAGGTCTGGATATGCCGGAAGTGTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCt < 1:2553967/69‑1 (MQ=255)
cgAAGGTCTGGATATGCCGGAAGTTTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGc < 1:1896159/68‑1 (MQ=255)
cgAAGGTCTGGATATGCCGGAAGTTTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGc < 1:3429337/68‑1 (MQ=255)
cgAAGGTCTGGATATGCCGGAAGTTTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGc < 1:2394118/68‑1 (MQ=255)
cgAAGGTCTGGATATGCCGGAAGTTTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGc < 1:535281/68‑1 (MQ=255)
cgAAGGTCTGGATATGCCGGAAGTTTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGc < 1:2159006/68‑1 (MQ=255)
cgAAGGTCTGGATATGCCGGAAGTTTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGc < 1:664709/68‑1 (MQ=255)
cgAAGGTCTGGATATGCCGGAAGTTTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGc < 1:1871919/68‑1 (MQ=255)
cgAAGGTCTGGATATGCCGGAAGTTTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGc < 1:18297/68‑1 (MQ=255)
cgAAGGTCTGGATATGCCGGAAGTTTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGc < 1:3126705/68‑1 (MQ=255)
cgAAGGTCTGGATATGCCGGAAGTTTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGc < 1:3126163/68‑1 (MQ=255)
cgAAGGTCTGGATATGCCGGAAGTTTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGc < 1:3069952/68‑1 (MQ=255)
cgAAGGTCTGGATATGCCGGAAGTTTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGc < 1:3074237/68‑1 (MQ=255)
atGCCGGAAGTGTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGCGTTCCGAAcgttcg > 1:2237834/1‑69 (MQ=255)
atGCCGGAAGTGTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGCGTTCCGAAcgttcg > 1:3074592/1‑69 (MQ=255)
atGCCGGAAGTGTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGCGTTCCGAAcgttcg > 1:516354/1‑69 (MQ=255)
atGCCGGAAGTGTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGCGTTCCGAAcgttcg > 1:1842557/1‑69 (MQ=255)
atGCCGGAAGTGTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGCGTTCCGAAcgttcg > 1:1821867/1‑69 (MQ=255)
atGCCGGAAGTGTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGCGTTCCGAAcgttcg > 1:912194/1‑69 (MQ=255)
atGCCGGAAGTGTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGCGTTCCGAAcgttc > 1:1622626/1‑68 (MQ=255)
aGTTTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGCGTTCCGAACGTTCGTTGATCCa > 1:462329/1‑69 (MQ=255)
aGTTTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGCGTTCCGAACGTTCGTTGATCCa > 1:2754085/1‑69 (MQ=255)
aGTTTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGCGTTCCGAACGTTCGTTGATCCa > 1:3281145/1‑69 (MQ=255)
aGTTTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGCGTTCCGAACGTTCGTTGATCCa > 1:743323/1‑69 (MQ=255)
aGTTTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGCGTTCCGAACGTTCGTTGATCCa > 1:97019/1‑69 (MQ=255)
aGTTTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGCGTTCCGAACGTTCGTTGATCCa > 1:3003588/1‑69 (MQ=255)
aGTTTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGCGTTCCGAACGTTCGTTGATCCa > 1:2995875/1‑69 (MQ=255)
aGTTTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGCGTTCCGAACGTTCGTTGATCCa > 1:2902810/1‑69 (MQ=255)
aGTTTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGCGTTCCGAACGTTCGTTGATCCa > 1:2901120/1‑69 (MQ=255)
aGTTTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGCGTTCCGAACGTTCGTTGATCCa > 1:2673552/1‑69 (MQ=255)
aGTTTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGCGTTCCGAACGTTCGTTGATCCa > 1:2591527/1‑69 (MQ=255)
aGTTTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGCGTTCCGAACGTTCGTTGATCCa > 1:231209/1‑69 (MQ=255)
aGTTTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGCGTTCCGAACGTTCGTTGATCCa > 1:2239169/1‑69 (MQ=255)
aGTTTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGCGTTCCGAACGTTCGTTGATCCa > 1:2018021/1‑69 (MQ=255)
aGTTTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGCGTTCCGAACGTTCGTTGATCCa > 1:1940492/1‑69 (MQ=255)
aGTTTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGCGTTCCGAACGTTCGTTGATCCa > 1:1713563/1‑69 (MQ=255)
aGTTTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGCGTTCCGAACGTTCGTTGATCCa > 1:1689149/1‑69 (MQ=255)
aGTTTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGCGTTCCGAACGTTCGTTGATCCa > 1:1541879/1‑69 (MQ=255)
aGTTTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGCGTTCCGAACGTTCGTTGATCCa > 1:1220986/1‑69 (MQ=255)
aGTTTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGCGTTCCGAACGTTCGTTGATCCa > 1:1213938/1‑69 (MQ=255)
|
GATCAACTTACTGCGCGAAGGTCTGGATATGCCGGAAGTGTCGCTGGTGGCGATCCTCGACGCTGACAAAGAAGGCTTCCTGCGTTCCGAACGTTCGTTGATCCA > minE/521486‑521590
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
