Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
523,603 |
C→T |
35.5% |
G35G (GGC→GGT) |
moaA → |
molybdopterin biosynthesis protein A |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 523,603 | 0 | C | T | 35.5%
| 75.2
/ 60.3
| 104 | G35G (GGC→GGT) | moaA | molybdopterin biosynthesis protein A |
| Reads supporting (aligned to +/- strand): ref base C (30/37); new base T (19/18); total (49/55) |
| Fisher's exact test for biased strand distribution p-value = 5.45e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.32e-01 |
Only 100 of 111 total aligned reads displayed.
TTTTACTACTTGCGCCTGTCGATTACCGATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTACGCG > minE/523535‑523668
|
ttttACTACTTGCGCCTGTCGATTACCGATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGATGGc < 1:2501686/69‑1 (MQ=255)
ttttACTACTTGCGCCTGTCGATTACCGATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGATGGc < 1:3328241/69‑1 (MQ=255)
ttttACTACTTGCGCCTGTCGATTACCGATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGATGGc < 1:283932/69‑1 (MQ=255)
ttttACTACTTGCGCCTGTCGATTACCGATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGATGGc < 1:641197/69‑1 (MQ=255)
ttttACTACTTGCGCCTGTCGATTACCGATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGATGGc < 1:1578733/69‑1 (MQ=255)
ctTGCGCCTGTCGATTACCGATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGATGGCTACaaa > 1:841572/1‑67 (MQ=255)
ctTGCGCCTGTCGATTACCGATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGATGGCTACaaa > 1:338430/1‑67 (MQ=255)
tCGATTACTGATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGATGGTTACAAGCCGAGCGGCTTc > 1:2043564/1‑69 (MQ=255)
tCGATTACTGATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGATGGTTACAAGCCGAGCGGCGTc > 1:636461/1‑69 (MQ=255)
tCGATTACTGATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGATGGTTACAAGCCGAGCGGCGTc > 1:3248473/1‑69 (MQ=255)
tCGATTACTGATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGATGGTTACAAGCCGAGCGGCGTc > 1:2879815/1‑69 (MQ=255)
tCGATTACTGATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGATGGTTACAAGCCGAGCGGCGTc > 1:1451989/1‑69 (MQ=255)
gATTACCGATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGATGGCTACAAACCGAGCGGCGTCa > 1:1861858/1‑68 (MQ=255)
tACCGATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGATGGCTACAAACCGAGCGGCGt < 1:2562805/63‑1 (MQ=255)
tACCGATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGATGGCTACAAACCGAGCGGCGt < 1:1346037/63‑1 (MQ=255)
tACCGATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGATGGCTACAAACCGAGCGGCGt < 1:853067/63‑1 (MQ=255)
tACCGATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGATGGCTACAAACCGAGCGGCGt < 1:795582/63‑1 (MQ=255)
tACCGATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGATGGCTACAAACCGAGCGGCGt < 1:2663842/63‑1 (MQ=255)
tACCGATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGATGGCTACAAACCGAGCGGCGt < 1:2503464/63‑1 (MQ=255)
tACCGATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGATGGCTACAAACCGAGCGGCGt < 1:1872618/63‑1 (MQ=255)
tACCGATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGATGGCTACAAACCGAGCGGCGt < 1:2242183/63‑1 (MQ=255)
gATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGATGGTTACAAGCCGAGCGGCGTCACCATTaaa < 1:1313295/69‑1 (MQ=255)
gATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGATGGTTACAAGCCGAGCGGCGTCACCAATaaa < 1:1993288/69‑1 (MQ=255)
gATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGATGGTTACAAGCCGAGCGGCGTCACCAATaaa < 1:2505961/69‑1 (MQ=255)
gATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGATGGTTACAAGCCGAGCGGCGTCACCAATaaa < 1:276969/69‑1 (MQ=255)
gATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGATGGTTACAAGCCGAGCGGCGTCACCAATaaa < 1:2094253/69‑1 (MQ=255)
gATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGATGGTTACAAGCCGAGCGGCGTCACCAATaaa < 1:2581469/69‑1 (MQ=255)
gATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGATGGTTACAAGCCGAGCGGCGTCACCAATaaa < 1:2133298/69‑1 (MQ=255)
gATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGATGGTTACAAGCCGAGCGGCGTCACCAATaaa < 1:2324128/69‑1 (MQ=255)
gATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGATGGTTACAAGCCGAGCGGCGTCACCAATaaa < 1:322542/69‑1 (MQ=255)
gATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGATGGTTACAAGCCGAGCGGCGTCACCAATaaa < 1:484209/69‑1 (MQ=255)
gATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGATGGTTACAAGCCGAGCGGCGTCACCAATaaa < 1:60510/69‑1 (MQ=255)
gATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGATGGTTACAAGCCGAGCGGCGTCACCAATaaa < 1:1308428/69‑1 (MQ=255)
gATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGATGGTTACAAGCCGAGCGGCGTCACCAATaaa < 1:1299777/69‑1 (MQ=255)
gATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGATGGTTACAAGCCGAGCGGCGTCACCAATaaa < 1:752604/69‑1 (MQ=255)
gATGTGTGTAACTTTCGTTGCACATACTGCCTGCCGGATGGTTACAAGCCGAGCGGCGTCACCAATata < 1:1719190/69‑3 (MQ=255)
gATGTGTGTAACTTTCGTTGAACCTACTGCCTGCCGGATGGTTACAAGCCGAGCGGCGTCACCAATaaa < 1:3392887/69‑1 (MQ=255)
aTGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGATGGTTACAAGCCGAGCGGCGTCACCAATaaa < 1:1788939/68‑1 (MQ=255)
tgtgtAACTTTCGTTGCACCTACTGCCTGCCGGATGGCTa > 1:524093/1‑40 (MQ=255)
tgtgtAACTTTCGTTGCACCTACTGCCTGCCGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCt > 1:3367257/1‑69 (MQ=255)
tgtgtAACTTTCGTTGCACCTACTGCCTGCCGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCt > 1:3379604/1‑69 (MQ=255)
tgtgtAACTTTCGTTGCACCTACTGCCTGCCGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCt > 1:643912/1‑69 (MQ=255)
tgtgtAACTTTCGTTGCACCTACTGCCTGCCGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCt > 1:2529525/1‑69 (MQ=255)
tgtgtAACTTTCGTTGCACCTACTGCCTGCCGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCt > 1:878097/1‑69 (MQ=255)
tgtgtAACTTTCGTTGCACCTACTGCCTGCCGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCt < 1:1069618/69‑1 (MQ=255)
tgtgtAACTTTCGTTGCACCTACTGCCTGCCGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCt > 1:1428249/1‑69 (MQ=255)
tgtgtAACTTTCGTTGCACCTACTGCCTGCCGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCt > 1:1854174/1‑69 (MQ=255)
tgtgtAACTTTCGTTGCACCTACTGCCTGCCGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCt > 1:199023/1‑69 (MQ=255)
tgtgtAACTTTCGTTGCACCTACTGCCTGCCGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCt > 1:1081209/1‑69 (MQ=255)
tgtAACTTTCGTTGCACCTACTGCCTGCCGGATGGc < 1:2100578/36‑1 (MQ=255)
tgtAACTTTCGTTGCACCTACTGCCTGCCGGATGGCTACa > 1:2838590/1‑40 (MQ=255)
tgtAACTTTCGTTGCACCTACTGCCTGCCGGATGGCTACAAAcc > 1:313445/1‑44 (MQ=255)
tgtAACTTTCGTTGCACCTACTGCCTGCCGGATGGCTACAAACCg > 1:3361475/1‑45 (MQ=255)
tgtAACTTTCGTTGCACCTACTGCCTGCCGGATGGCTACAAACCg > 1:2216754/1‑45 (MQ=255)
tgtAACTTTCGTTGCACCTACTGCCTGCCGGATGGCTACAAACCg > 1:2020901/1‑45 (MQ=255)
tgtAACTTTCGTTGCACCTACTGCCTGCCGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCttt < 1:2311496/69‑1 (MQ=255)
tgtAACTTTCGTTGCACCTACTGCCTGCCGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCttt < 1:3434051/69‑1 (MQ=255)
tgtAACTTTCGTTGCACCTACTGCCTGCCGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCttt < 1:3323157/69‑1 (MQ=255)
tAACTTTCGTTGCACCTACTGCCTGCCGGATGGTTACAAGCCGAGCGGCGTCACCAATAAAGGCTTTc > 1:732674/1‑68 (MQ=255)
tAACTTTCGTTGCACCTACTGCCTGCCGGATGGTTACAAGCCGAGCGGCGTCACCAATAAAGGCTTTCt > 1:2720558/1‑69 (MQ=255)
tAACTTTCGTTGCACCTACTGCCTGCCGGATGGTTACAAGCCGAGCGGCGTCACCAATAAAGGCTTTCt > 1:1943368/1‑69 (MQ=255)
tAACTTTCGTTGCACCTACTGCCTGCCGGATGGTTACAAGCCGAGCGGCGTCACCAATAAAGGCTTTCt > 1:2164530/1‑69 (MQ=255)
tAACTTTCGTTGCACCTACTGCCTGCCGGATGGTTACAAGCCGAGCGGCGTCACCAATAAAGGCTTTCt > 1:178419/1‑69 (MQ=255)
tAACTTTCGTTGCACCTACTGCCTGCCGGATGGTTACAAGCCGAGCGGCGTCACCAATAAAGGCTTTCt > 1:3262387/1‑69 (MQ=255)
tAACTTTCGTTGCACCTACTGCCTGCCGGATGGTTACAAGCCGAGCGGCGTCACCAATAAAGGCTTTCt > 1:559583/1‑69 (MQ=255)
tAACTTTCGTTGCACCTACTGCCTGCCGGATGGTTACAAGCCGAGCGGCGTCACCAATAAAGGCTTTCt > 1:593194/1‑69 (MQ=255)
tAACTTTCGTTGCACCTACTGCCTGCCGGATGGTTACAAGCCGAGCGGCGTCACCAATAAAGGCTTTCt > 1:2595162/1‑69 (MQ=255)
tAACTTTCGTTGCACCTACTGCCTGCCGGATGGTTACAAGCCGAGCGGCGTCACCAATAAAGGCTTTCt > 1:2888113/1‑69 (MQ=255)
tAACTTTCGTTGCACCTACTGCCTGCCGGATGGTTACAAGCCGAGCGGCGTCACCAATAAAGGCTTTCt > 1:161076/1‑69 (MQ=255)
tAACTTTCGTTGCACCTACTGCCTGCCGGATGGTTACAAGCCGAGCGGCGTCACCAATAAAGGCTTTCt > 1:2342221/1‑69 (MQ=255)
tAACTTTCGTTGCACCTACTGCCTGCCGGATGGTTACAAGCCGAGCGGCGTCACCAATAAAGGCTTTCt > 1:2785236/1‑69 (MQ=255)
aCTTTCGTTGCACCTACTGCCTGCCGGATGGCTACAAACCTAGCGGCGTCACCAATAAAGGCTTTCtt < 1:1973863/68‑1 (MQ=255)
aCTTTCGTTGCACCTACTGCCTGCCGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCtt < 1:1693156/68‑1 (MQ=255)
aCTTTCGTTGCACCTACTGCCTGCCGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCtt < 1:1734674/68‑1 (MQ=255)
aCTTTCGTTGCACCTACTGCCTGCCGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCtt < 1:66522/68‑1 (MQ=255)
aCTTTCGTTGCACCTACTGCCTGCCGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCtt < 1:2508044/68‑1 (MQ=255)
aCTTTCGTTGCACCTACTGCCTGCCGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCtt < 1:2279934/68‑1 (MQ=255)
aCTTTCGTTGCACCTACTGCCTGCCGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCtt < 1:2741645/68‑1 (MQ=255)
aCTTTCGTTGCACCTACTGCCTGCCGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCtt < 1:3304073/68‑1 (MQ=255)
aCTTTCGTTGCACCTACTGCCTGCCGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCtt < 1:2110516/68‑1 (MQ=255)
aCTTTCGTTGCACCTACTGCCTGCCGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCtt < 1:3172592/68‑1 (MQ=255)
aCTTTCGTTGCACCTACTGCCTGCCGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCtt < 1:871775/68‑1 (MQ=255)
aCTTTCGTTGCACCTACTGCCTGCCGGATGGCTACAAACCGAGCGGCGTCACCAACAAAGGCTTTCtt < 1:1647970/68‑1 (MQ=255)
gcctgccGGATGGCTACAAACCTAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGa < 1:3320237/61‑1 (MQ=255)
gcctgccGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGa < 1:1936672/61‑1 (MQ=255)
gcctgccGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGa < 1:1123397/61‑1 (MQ=255)
gcctgccGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGa < 1:191690/61‑1 (MQ=255)
gcctgccGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGa < 1:2270900/61‑1 (MQ=255)
gcctgccGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGa < 1:1274132/61‑1 (MQ=255)
gcctgccGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGa < 1:3130544/61‑1 (MQ=255)
gcctgccGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGa < 1:2633675/61‑1 (MQ=255)
gcctgccGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGa < 1:2697785/61‑1 (MQ=255)
gcctgccGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGAAATTCGcc > 1:10264/1‑69 (MQ=255)
gcctgccGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGAAATTCGcc > 1:679959/1‑69 (MQ=255)
gcctgccGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGAAATTCGcc > 1:192561/1‑69 (MQ=255)
gcctgccGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGAAATTCGcc > 1:2000804/1‑69 (MQ=255)
gcctgcaGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGAAATTCGcc > 1:436070/1‑69 (MQ=255)
tGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCTTACCGt > 1:2005041/1‑45 (MQ=255)
tGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTAcgcg > 1:1123674/1‑69 (MQ=255)
tGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTAcgcg > 1:2411911/1‑69 (MQ=255)
|
TTTTACTACTTGCGCCTGTCGATTACCGATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGATGGCTACAAACCGAGCGGCGTCACCAATAAAGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTACGCG > minE/523535‑523668
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
