Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
552,642 |
G→A |
64.5% |
Y294Y (TAC→TAT) |
ybiW ← |
predicted pyruvate formate lyase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 552,642 | 0 | G | A | 64.5%
| 47.9
/ 44.3
| 76 | Y294Y (TAC→TAT) | ybiW | predicted pyruvate formate lyase |
| Reads supporting (aligned to +/- strand): ref base G (7/20); new base A (24/25); total (31/45) |
| Fisher's exact test for biased strand distribution p-value = 5.65e-02 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.22e-01 |
CAGCATCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACGGGTAGAGATACTGGTCCATACGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCA > minE/552576‑552706
|
cagcaTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACggg > 1:3043837/1‑67 (MQ=255)
cagcaTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACggg > 1:1884032/1‑67 (MQ=255)
cagcaTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACggg > 1:3068446/1‑67 (MQ=255)
cagcaTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACggg > 1:360069/1‑67 (MQ=255)
cagcaTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACggg > 1:2919035/1‑67 (MQ=255)
cagcaTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACggg > 1:1858812/1‑67 (MQ=255)
cagcaTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACggg > 1:2772418/1‑67 (MQ=255)
cagcaTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACggg > 1:796820/1‑67 (MQ=255)
cagcaTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACGGGTa < 1:2039974/69‑1 (MQ=255)
cagcaTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACGGGTa < 1:732180/69‑1 (MQ=255)
cagcaTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACGGGTa < 1:336475/69‑1 (MQ=255)
cagcaTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACGGGTa < 1:2874359/69‑1 (MQ=255)
agcaTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACGGGTa < 1:2187334/68‑1 (MQ=255)
aTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACGGGTAgaga < 1:311646/69‑1 (MQ=255)
aTCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACGGGTAgaga < 1:2266969/69‑1 (MQ=255)
gCGTGTTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATagag > 1:1814285/1‑59 (MQ=255)
gCGTGTTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGAt > 1:3182202/1‑61 (MQ=255)
gCGTGTTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTgtt > 1:2258843/1‑65 (MQ=255)
gCGTGTTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTc > 1:2765119/1‑68 (MQ=255)
gCGTGTTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTc > 1:3215557/1‑68 (MQ=255)
gCGTGTTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTc > 1:2672397/1‑68 (MQ=255)
gCGTGTTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTc > 1:3270680/1‑68 (MQ=255)
gCGTGTTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTc > 1:285360/1‑68 (MQ=255)
gCGTGTTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTc > 1:521025/1‑68 (MQ=255)
gCGTGTTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTc > 1:3051598/1‑68 (MQ=255)
gCGTGTTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTc > 1:2176948/1‑68 (MQ=255)
gCGTGTTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTc > 1:1878207/1‑68 (MQ=255)
gCGTGTTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTc > 1:771867/1‑68 (MQ=255)
gCGTGTTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTc > 1:1458542/1‑68 (MQ=255)
gCGTGTTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTc > 1:1431766/1‑68 (MQ=255)
gCGTGTTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTc > 1:753528/1‑68 (MQ=255)
gCGTGTTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTc > 1:1412836/1‑68 (MQ=255)
gCGTGTTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTc > 1:1404161/1‑68 (MQ=255)
gCGTGTTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTc > 1:1306149/1‑68 (MQ=255)
gCGTGTTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTc > 1:1859705/1‑68 (MQ=255)
gCGTGTTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTc > 1:731751/1‑68 (MQ=255)
gCGTGTTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTc > 1:987987/1‑68 (MQ=255)
gCGTGTTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTc > 1:111101/1‑68 (MQ=255)
gCGTGTTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTc > 1:677928/1‑68 (MQ=255)
gtTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGATATACTGGTCCATAc < 1:557936/69‑1 (MQ=255)
gtTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGTTACTGGTCCATAc < 1:1059807/69‑1 (MQ=255)
gtTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTCCATAc < 1:1761593/69‑1 (MQ=255)
gtTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTCCATAc < 1:92417/69‑1 (MQ=255)
gtTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTCCATAc < 1:3220422/69‑1 (MQ=255)
gtTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTCCATAc < 1:2104811/69‑1 (MQ=255)
gtTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTCCATAc < 1:25562/69‑1 (MQ=255)
gtTCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTCCATAc < 1:2251906/69‑1 (MQ=255)
ttCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTCCATAc < 1:2540282/68‑1 (MQ=255)
ttCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTCCATAc < 1:2468129/68‑1 (MQ=255)
ttCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTCCATAc < 1:680714/68‑1 (MQ=255)
ttCGCGATCCAGTGTCTGGTTCAGTTCAACGTCACGGCGATAGTATGGATAGAGATACTGGTCCATAc < 1:570488/68‑1 (MQ=255)
ttCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACGGGTAGAGATACTGGTCCATACg < 1:1737838/69‑1 (MQ=255)
ttCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACGGGTAGAGATACTGGTCCATACg < 1:1301486/69‑1 (MQ=255)
tCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACGGGTAGAGATACTGGTCCATACg < 1:1080531/68‑1 (MQ=255)
cgcgATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACGGGTAGAGATACTGGTCCATACGa > 1:1191639/1‑68 (MQ=255)
cgcgATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACGGGTAGAGATACTGGTCCATACGa > 1:3048753/1‑68 (MQ=255)
cgcgATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACGGGTAGAGATACTGGTCCATACGa > 1:1570941/1‑68 (MQ=255)
cgcgATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACGGGTAGAGATACTGGTCCATACGa > 1:1857697/1‑68 (MQ=255)
cgcgATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACGGGTAGAGATACTGGTCCATACGa > 1:2320135/1‑68 (MQ=255)
gCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACGGGTAGAGATACTGGTCCATACGACCAAACGAt < 1:825260/68‑1 (MQ=255)
cAACGTCGCGGCGATAGTACGGGTAGAGATACTGGTCCATACGACCAAACGa < 1:1553478/52‑1 (MQ=255)
cAACGTCGCGGCGATAGTACGGGTAGAGATACTGGTCCATACGACCAAACGa < 1:3181928/52‑1 (MQ=255)
cAACGTCGCGGCGATAGTACGGGTAGAGATACTGGTCCATACGACCAAACGATACTGAGt > 1:2256545/1‑60 (MQ=255)
cAACGTCGCGGCGATAGTACGGGTAGAGATACTGGTCCATACGACCAAACGATACTGAGTGAc > 1:2423510/1‑63 (MQ=255)
tcacggcgATAGTATGGATAGAGATACTGGTCCATACGACCAAACGATACCGAGTGACCGTTAGATTCg < 1:3044464/66‑1 (MQ=255)
tcacggcgATAGTATGGATAGAGATACTGGTCCATACGACCAAACGATACCGAGTGACCGTTAGATTCg < 1:2002041/66‑1 (MQ=255)
tcacggcgATAGTATGGATAGAGATACTGGTCCATACGACCAAACGATACCGAGTGACCGTTAGATTCg < 1:2015891/66‑1 (MQ=255)
tcacggcgATAGTATGGATAGAGATACTGGTCCATACGACCAAACGATACCGAGTGACCGTTAGATTCg < 1:2578753/66‑1 (MQ=255)
tcacggcgATAGTATGGATAGAGATACTGGTCCATACGACCAAACGATACCGAGTGACCGTTAGATTCg < 1:3029292/66‑1 (MQ=255)
tcacggcgATAGTATGGATAGAGATACTGGTCCATACGACCAAACGATACCGAGTGACCGTTAGATTCg < 1:2955335/66‑1 (MQ=255)
tcacggcgATAGTATGGATAGAGATACTGGTCCATACGACCAAACGATACCGAGTGACCGTTAGATTCg < 1:281923/66‑1 (MQ=255)
tcacggcgATAGTATGGATAGAGATACTGGTCCATACGACCAAACGATACCGAGTGACCGTTAGATTCg < 1:268360/66‑1 (MQ=255)
tcacggcgATAGTATGGATAGAGATACTGGTCCATACGACCAAACGATACCGAGTGACCGTTAGATTCg < 1:2676572/66‑1 (MQ=255)
tcacggcgATAGTATGGATAGAGATACTGGTCCATACGACCAAACGATACCGAGTGACCGTTAGATTCg < 1:3362800/66‑1 (MQ=255)
tcacggcgATAGTATGGATAGAGATACTGGTCCATACGACCAAACGATACCGAGTGACCGTTAGATTCg < 1:3369700/66‑1 (MQ=255)
cgcgGCGATAGTACGGGTAGAGATACTGGTCCATACGACCAAACGATACTGAGTGACCGTTAGATTCGa < 1:2306229/69‑1 (MQ=255)
cgcgGCGATAGTACGGGTAGAGATACTGGTCCATACGACCAAACGATACTGAGTGACCGTTAGATTCGa < 1:1763854/69‑1 (MQ=255)
cgcgGCGATAGTACGGGTAGAGATACTGGTCCATACGACCAAACGATACTGAGTGACCGTTAGATTCGa < 1:2949840/69‑1 (MQ=255)
cgcgGCGATAGTACGGGTAGAGATACTGGTCCATACGACCAAACGATACTGAGTGACCGTTAGATTCGa < 1:406181/69‑1 (MQ=255)
cacggcgATAGTATGGATAGAGATACTGGTCCATACGACCAAACGATACCGAGTGACCGTTAGATTCg < 1:2948374/66‑1 (MQ=255)
gcggcgATAGTACGGGTAGAGATACTGGTCCATACGACCAAACGATACTGAGTGACCGTTAGATTCGa < 1:1196037/68‑1 (MQ=255)
acggcgATAGTATGGATAGAGATACTGGTCCATACGACCAAACGATACCGAGTGACCGTTAGATTCg < 1:2591301/66‑1 (MQ=255)
aCGGGTAGAGATACTGGTCCATACGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCa < 1:1888020/69‑1 (MQ=255)
aCGGGTAGAGATACTGGTCCATACGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCa < 1:950838/69‑1 (MQ=255)
|
CAGCATCTCGATGGCGTGTTCGCGATCCAGCGTCTGGTTGAGTTCAACGTCGCGGCGATAGTACGGGTAGAGATACTGGTCCATACGACCAAACGATACTGAGTGACCGTTAGATTCGATCTGCAAAATCA > minE/552576‑552706
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
