Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
577,604 |
C→T |
56.9% |
L187L (CTG→TTG) |
macB → |
fused macrolide transporter subunits and ATP‑binding component and membrane component of ABC superfamily |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 577,604 | 0 | C | T | 56.9%
| 9.9
/ 37.7
| 51 | L187L (CTG→TTG) | macB | fused macrolide transporter subunits and ATP‑binding component and membrane component of ABC superfamily |
| Reads supporting (aligned to +/- strand): ref base C (12/10); new base T (19/10); total (31/20) |
| Fisher's exact test for biased strand distribution p-value = 5.64e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01 |
GTAATTCTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGAAGAGGTGATGGCGATCCTGCATCAGCTGCGCGATCGTGGGCATACGGTGATTATCGTCACCCACGATCCGCAGGTCGCTGCTCA > minE/577538‑577671
|
gTAATTCTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGAAGAGGTGATGGCGATCCTg > 1:998246/1‑69 (MQ=255)
gTAATTCTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGAAGAGGTGATGGCGATCCTg > 1:1818372/1‑69 (MQ=255)
cTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGAAGAGGTGATGGCGATCCTGCATCAg > 1:2363654/1‑69 (MQ=255)
gACAGCCATTCTGGCGAAGAGGTGATGGCGATCCTGCATCAGCt > 1:2662450/1‑44 (MQ=255)
gACAGCCATTCTGGCGAAGAGGTGATGGCGATCCTGCATCAGCTGCGCGATCGTGGGCATACGGTGAtt > 1:2717557/1‑69 (MQ=255)
gACAGCCATTCTGGCGAAGAGGTGATGGCGATCCTGCATCAGCTGCGCGATCGTGGGCATACGGTGAt > 1:1237645/1‑68 (MQ=255)
aGCCATTCTGGCGAAGAGGTGATGGCGATCCTGCATCAGCTGCGCGATCGTGGGCATACGGTGATTATc < 1:13267/69‑1 (MQ=255)
cATTCTGGCGAAGAGGTGATGGCGATCCTGCATCAGCTGCGCGATc > 1:628131/1‑46 (MQ=255)
cATTCTGGCGAAGAGGTGATGGCGATCCTGCATCAGCTGCGCGATCGTGGGCATACGGTGATTATCGt > 1:545064/1‑68 (MQ=255)
cATTCTGGCGAAGAGGTGATGGCGATCCTGCATCAGCTGCGCGATCGTGGGCATACGGTGATTATCGTc > 1:383587/1‑69 (MQ=255)
cATTCTGGCGAAGAGGTGATGGAGATCCTGCATCAGCTGCGCGATCGTGGGCATACGGTGATTATCGTc > 1:2498035/1‑69 (MQ=255)
ttCTGGCGAAGAGGTGATGGCGATCTTGCATCAGCTGCGCGATCGCTGGCATACGGTGAtt > 1:212688/1‑61 (MQ=255)
ttCTGGCGAAGAGGTGATGGCGATCTTGCATCAGCTGCGCGATCGCGTGCATACGGTGAtt > 1:1823053/1‑61 (MQ=38)
ttCTGGCGAAGAGGTGATGGCGATCTTGCATCAGCTGCGCGATCGCGGGCATACGGTGAtt > 1:3089923/1‑61 (MQ=39)
ttCTGGCGAAGAGGTGATGGCGATCTTGCATCAGCTGCGCGATCGCGGGCATACGGTGAtt > 1:3016162/1‑61 (MQ=39)
ttCTGGCGAAGAGGTGATGGCGATCTTGCATCAGCTGCGCGATCGCGGGCATACGGTGAtt > 1:2917859/1‑61 (MQ=39)
ttCTGGCGAAGAGGTGATGGCGATCTTGCATCAGCTGCGCGATCGCGGGCATACGGTGAtt > 1:2767699/1‑61 (MQ=39)
ttCTGGCGAAGAGGTGATGGCGATCTTGCATCAGCTGCGCGATCGCGGGCATACGGTGAtt > 1:3374064/1‑61 (MQ=39)
ttCTGGCGAAGAGGTGATGGCGATCTTGCATCAGCTGCGCGATCGCGGGCATACGGTGAtt > 1:412384/1‑61 (MQ=39)
ttCTGGCGAAGAGGTGATGGCGATCTTGCATCAGCTGCGCGATCGCGGGCATACGGTGAtt > 1:564225/1‑61 (MQ=39)
ttCTGGCGAAGAGGTGATGGCGATCTTGCATCAGCTGCGCGATCGCGGGCATACGGTGAtt > 1:259383/1‑61 (MQ=39)
ttCTGGCGAAGAGGTGATGGCGATCTTGCATCAGCTGCGCGATCGCGGGCATACGGTGAtt > 1:2540527/1‑61 (MQ=39)
ttCTGGCGAAGAGGTGATGGCGATCTTGCATCAGCTGCGCGATCGCGGGCATACGGTGAtt > 1:866229/1‑61 (MQ=39)
ttCTGGCGAAGAGGTGATGGCGATCTTGCATCAGCTGCGCGATCGCGGGCATACGGTGAtt > 1:1014604/1‑61 (MQ=39)
ttCTGGCGAAGAGGTGATGGCGATCTTGCATCAGCTGCGCGATCGCGGGCATACGGTGAtt > 1:1009774/1‑61 (MQ=39)
ttCTGGCGAAGAGGTGATGGCGATCTTGCATCAGCTGCGCGATCGCGGGCATACGGTGAtt > 1:1523351/1‑61 (MQ=39)
ttCTGGCGAAGAGGTGATGGCGATCTTGCATCAGCTGCGCGATCGCGGGCATACGGTGAtt > 1:1630821/1‑61 (MQ=39)
ttCTGGCGAAGAGGTGATGGCGATCTTGCATCAGCTGCGCGATCGCGGGCATACGGTGAtt > 1:1864516/1‑61 (MQ=39)
ttCTGGCGAAGAGGTGATGGCGATCTTGCATCAGCTCCGCGATCGCGGGCAAACGGTGATTa > 1:2175977/1‑62 (MQ=255)
ttCTCGCGAAGAGGTGATGGCGATCTTGCATCAGCTGCGCGATCGCGGGCATACGGTGAtt > 1:341366/1‑61 (MQ=38)
agagGTGATGGCGATCTTGCATCAGCTGCGCGATCGCGGGCATACGGTGa < 1:263658/50‑1 (MQ=38)
agagGTGATGGCGATCTTGCATCAGCTGCGCGATCGCGGGCATACGGTGa < 1:2926318/50‑1 (MQ=38)
agagGTGATGGCGATCTTGCATCAGCTGCGCGATCGCGGGCATACGGTGa < 1:942489/50‑1 (MQ=38)
agagGTGATGGCGATCTTGCATCAGCTGCGCGATCGCGGGCATACGGTGa < 1:1447525/50‑1 (MQ=38)
agagGTGATGGCGATCTTGCATCAGCTGCGCGATCGCGGGCATACGGTGa < 1:3344942/50‑1 (MQ=38)
agagGTGATGGCGATCTTGCATCAGCTGCGCGATCGCGGGCATACGGTGa < 1:1143941/50‑1 (MQ=38)
agagGTGATGGCGATCTTGCATCAGCTGCGCGATCGCGGGCATACGGTGa < 1:11500/50‑1 (MQ=38)
agagGTGATGGCGATCTTGCATCAGCTGCGCGATCGCGGGCATACGGTGa < 1:588242/50‑1 (MQ=38)
agagGTGATGGCGATCTTGCATCAGCTGCGCGATCGCGGGCATACGGTGa < 1:525761/50‑1 (MQ=38)
agagGTGATGGCGATCTTGCATCAGCTGCGCGATCGCGGGCATACGGTGa < 1:530443/50‑1 (MQ=38)
ggCGATCCTGCATCAGCTGCGCGATCGTGGGCATACGGTGATTATCGTCACCCACGATCCGCAGGTCgc > 1:2930397/1‑69 (MQ=255)
ggCGATCCTGCATCAGCTGCGCGATCGTGGGCATACGGTGATTATCGTCACCCACGATCCGCAGGTCgc > 1:1348718/1‑69 (MQ=255)
gATCCTGCATCAGCTGCGCGATCGTGGGCATACGGTGATTATCGTCACCCACGATCCGCAGGTCgctg < 1:408591/68‑1 (MQ=255)
gATCCTGCATCAGCTGCGCGATCGTGGGCATACGGTGATTATCGTCACCCACGATCCGCAGGTCgctg < 1:2288887/68‑1 (MQ=255)
gATCCTGCATCAGCTGCGCGATCGTGGGCATACGGTGATTATCGTCACCCACGATCCGCAGGTCgctg < 1:617741/68‑1 (MQ=255)
gATCCTGCATCAGCTGCGCGATCGTGGGCATACGGTGATTATCGTCACCCACGATCCGCAGGTCgctg < 1:2025380/68‑1 (MQ=255)
gATCCTGCATCAGCTGCGCGATCGTGGGCATACGGTGATTATCGTCACCCACGATCCGCAGGTCgctg < 1:1359773/68‑1 (MQ=255)
gATCCTGCATCAGCTGCGCGATCGTGGGCATACGGTGATTATCGTCACCCACGATCCGCAGGTCgctg < 1:1372404/68‑1 (MQ=255)
gATCCTGCATCAGCTGCGCGATCGTGGGCATACGGTGATTATCGTCACCCACGATCCGCAGGTCgctg < 1:1461172/68‑1 (MQ=255)
gATCCTGCATCAGCTGCGCGATCGTGGGCATACGGTGATTATCGTCACCCACGATCCGCAGGTCgctg < 1:2661846/68‑1 (MQ=255)
gATCCTGCATCAGCTGCGCGATCGCGGGCATACGGTGATTATCGTCACCCACGATCCGCAGGTCgctg < 1:1306503/68‑1 (MQ=255)
ccTGCATCAGCTGCGCGATCGTGGGCATACGGTGATTATCGTCACCCACGATCCGCAGGTCGCTGCTCa > 1:1354289/1‑69 (MQ=255)
|
GTAATTCTTGCCGATGAACCAACCGGCGCACTGGACAGCCATTCTGGCGAAGAGGTGATGGCGATCCTGCATCAGCTGCGCGATCGTGGGCATACGGTGATTATCGTCACCCACGATCCGCAGGTCGCTGCTCA > minE/577538‑577671
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
