Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
584,816 |
G→T |
60.4% |
S360Y (TCT→TAT) |
cydC ← |
fused cysteine transporter subunits and membrane component and ATP‑binding component of ABC superfamily |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 584,816 | 0 | G | T | 60.4%
| 16.2
/ 43.3
| 48 | S360Y (TCT→TAT) | cydC | fused cysteine transporter subunits and membrane component and ATP‑binding component of ABC superfamily |
| Reads supporting (aligned to +/- strand): ref base G (11/8); new base T (13/16); total (24/24) |
| Fisher's exact test for biased strand distribution p-value = 5.56e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01 |
AGTGTTGATTTGCCGCATCCGGTTCGCCCGAGAATCGCTATATGTTCCCCGGCGTTTACCTGAAGAGAAATCCCTTTAAGTGCCTGTTGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGA > minE/584750‑584883
|
aGTGTTGATTTGCCGCATCCGGTTCGCCCGAGAATCGCTATATGTTCCCCGGCGTTTACCTGAAGAGaa < 1:1100124/69‑1 (MQ=255)
aGTGTTGATTTGCCGCATCCGGTTCGCCCGAGAATCGCTATATGTTCCCCGGCGTTTACCTGAAGAGaa < 1:2023650/69‑1 (MQ=255)
aGTGTTGATTTGCCGCATCCGGTTCGCCCGAGAATCGCTATATGTTCCCCGGCGTTTACCTGAAGAGaa < 1:1286643/69‑1 (MQ=255)
gATTTGCCGCATCCGGTTCGCCCGAGAATCGCTATATGTTCCCCGGCGTTTACCTGAAGAGAAATccc > 1:949302/1‑68 (MQ=255)
tGCCGCATCCGGTTCGCCCGAGAATCGCTATATGTTCCCCGGCGTTTACCTGAagag > 1:966876/1‑57 (MQ=255)
tGCCGCATCCGGTTCGCCCGAGAATCGCTATATGTTCCCCGGCGTTTACCTGAAgaga > 1:1235738/1‑58 (MQ=255)
tGCCGCATCCGGTTCGCCCGAGAATCGCTATATGTTCCCCGGCGTTTACCTGAAgaga > 1:2865872/1‑58 (MQ=255)
tGCCGCATCCGGTTCGCCCGAGAATCGCTATATGTTCCCCGGCGTTTACCTGAAgaga > 1:2131580/1‑58 (MQ=255)
tGCCGCATCCGGTTCGCCCGAGAATCGCTATATGTTCCCCGGCGTTTACCTGAAgaga > 1:3161298/1‑58 (MQ=255)
tGCCGCATCCGGTTCGCCCGAGAATCGCTATATGTTCCCCGGCGTTTACCTGAAgaga > 1:1164450/1‑58 (MQ=255)
tGCCGCATCCGGTTCGCCCGAGAATCGCTATATGTTCCCCGGCGTTTACCTGAAgaga > 1:1941431/1‑58 (MQ=255)
tGCCGCATCCGGTTCGCCCGAGAATCGCTATATGTTCCCCGGCGTTTACCTGAAgaga > 1:1489610/1‑58 (MQ=255)
tGCCGCATCCGGTTCGCCCGAGAATCGCTATATGTTCCCCGGCGTTTACCTGAAgaga > 1:962986/1‑58 (MQ=255)
cGGTTCGCCCGAGAATCGCTATATGTTCCCCGGCGTTTACCTGAAGAGAAATCCCTTTAAGTGCCTg > 1:1682363/1‑67 (MQ=255)
cGGTTCGCCCGAGAATCGCTATATGTTCCCCGGCGTTTACCTGAAGAGAAATCCCTTTAAGTGCCTg > 1:866304/1‑67 (MQ=255)
cGGTTCGCCCGAGAATCGCTATATGTTCCCCGGCGTTTACCTGAAGAGAAATCCCTTTAAGTGCCTg > 1:2075250/1‑67 (MQ=255)
cGGTTCGCCCGAGAATCGCTATATGTTCCCCGGCGTTTACCTGAAGAGAAATCCCTTTAAGTGCCTg > 1:2914413/1‑67 (MQ=255)
ggTTCGCCCGAGAATCGCAATATGTTCCCCGGCGTTTACCTGAAGATAAATCCCttt > 1:688586/1‑57 (MQ=255)
ggTTCGCCCGAGAATCGCAATATGTTCCCCGGCGTTTACCTGAAGATAAATCCCttt > 1:113975/1‑57 (MQ=255)
ggTTCGCCCGAGAATCGCAATATGTTCCCCGGCGTTTACCTGAAGATAAATCCCttt > 1:61813/1‑57 (MQ=255)
ggTTCGCCCGAGAATCGCAATATGTTCCCCGGCGTTTACCTGAAGATAAATCCCttt > 1:2932022/1‑57 (MQ=255)
ggTTCGCCCGAGAATCGCAATATGTTCCCCGGCGTTTACCTGAAGATAAATCCCttt > 1:1226913/1‑57 (MQ=255)
ggTTCGCCCGAGAATCGCAATATGTTCCCCGGCGTTTACCTGAAGATAAATCCCttt > 1:2899566/1‑57 (MQ=255)
ggTTCGCCCGAGAATCGCAATATGTTCCCCGGCGTTTACCTGAAGATAAATCCCttt > 1:1046937/1‑57 (MQ=255)
ggTTCGCCCGAGAATCGCAATATGTTCCCCGGCGTTTACCTGAAGATAAATCCCttt > 1:2743267/1‑57 (MQ=255)
ggTTCGCCCGAGAATCGCAATATGTTCCCCGGCGTTTACCTGAAGATAAATCCCttt > 1:2588093/1‑57 (MQ=255)
ggTTCGCCCGAGAATCGCAATATGTTCCCCGGCGTTTACCTGAAGATAAATCCCttt > 1:2515739/1‑57 (MQ=255)
ggTTCGCCCGAGAATCGCAATATGTTCCCCGGCGTTTACCTGAAGATAAATCCCttt > 1:1845131/1‑57 (MQ=255)
ggTTCGCCCGAGAATCGCAATATGTTCCCCGGCGTTTACCTGAAGATAAATCCCttt > 1:2151806/1‑57 (MQ=255)
ggTTCGCCCGAGAATCGCAATATGTTCCCCGGCGTTTACCTGAAGATAAATCCCttt > 1:2093628/1‑57 (MQ=255)
gagaATCGCAATATGTTCCCCGGCGTTTACCTGAAGATAAATCCCTTTAAGTGCCTGTTGGGATTGCTc < 1:1299601/69‑1 (MQ=255)
gagaATCGCAATATGTTCCCCGGCGTTTACCTGAAGATAAATCCCTTTAAGTGCCTGTTGGGATTGCTc < 1:2464840/69‑1 (MQ=255)
gagaATCGCAATATGTTCCCCGGCGTTTACCTGAAGATAAATCCCTTTAAGTGCCTGTTGGGATTGCTc < 1:1539882/69‑1 (MQ=255)
gagaATCGCAATATGTTCCCCGGCGTTTACCTGAAGATAAATCCCTTTAAGTGCCTGTTGGGATTGCTc < 1:2716144/69‑1 (MQ=255)
gagaATCGCAATATGTTCCCCGGCGTTTACCTGAAGATAAATCCCTTTAAGTGCCTGTTGGGATTGCTc < 1:220931/69‑1 (MQ=255)
gagaATCGCAATATGTTCCCCGGCGTTTACCTGAAGATAAATCCCTTTAAGTGCCTGTTGGGATTGCTc < 1:2036439/69‑1 (MQ=255)
gagaATCGCAATATGTTCCCCGGCGTTTACCTGAAGATAAATCCCTTTAAGTGCCTGTTGGGATTGCTc < 1:482215/69‑1 (MQ=255)
gagaATCGCAATATGTTCCCCGGCGTTTACCTGAAGATAAATCCCTTTAAGTGCCTGTTGGGATTGCTc < 1:324056/69‑1 (MQ=255)
gcaatatTTTCCCCGGCGTTTACCTGAAGATAAATCCCTTTAAGTGCCTGTTGGGATTGCTc < 1:3115626/59‑1 (MQ=255)
tGTTCCCCGGCGTTTACCTGAAGAGAAATCCCTTTAAGTGCCTGTTGAGATTGCTCCGGATAAGtgaa < 1:1208402/68‑1 (MQ=255)
tGTTCCCCGGCGTTTACCTGAAGAGAAATCCCTTTAAGTGCCTGTTGAGATTGCTCCGGATAAGtgaa < 1:263834/68‑1 (MQ=255)
ggCGTTTACCTGAAGATAAATCCCTTTAAGTGCCTGTTGGGATTGCTCCGGATAAGTGAACTGAACATc < 1:3163524/69‑1 (MQ=255)
ggCGTTTACCTGAAGATAAATCCCTTTAAGTGCCTGTTGGGATTGCTCCGGATAAGTGAACTGAACATc < 1:2039717/69‑1 (MQ=255)
ggCGTTTACCTGAAGATAAATCCCTTTAAGTGCCTGTTGGGATTGCTCCGGATAAGTGAACTGAACATc < 1:404235/69‑1 (MQ=255)
ggCGTTTACCTGAAGATAAATCCCTTTAAGTGCCTGTTGGGATTGCTCCGGATAAGTGAACTGAACATc < 1:518216/69‑1 (MQ=255)
ggCGTTTACCTGAAGATAAATCCCTTTAAGTGCCTGTTGGGATTGCTCCGGATAAGTGAACTGAACATc < 1:2097561/69‑1 (MQ=255)
ggCGTTTACCTGAAGATAAATCCCTTTAAGTGCCTGTTGGGATTGCTCCGGATAAGTGAACTGAACATc < 1:227732/69‑1 (MQ=255)
ggCGTTTACCTGAAGATAAATCCCTTTAAGTGCCTGTTGGGATTGCTCCGGATAAGTGAACTGAACATc < 1:724596/69‑1 (MQ=255)
tACCTGAAGAGAAATCCCTTTAAGTGCCTGTTGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTaa > 1:2550794/1‑69 (MQ=255)
tACCTGAAGAGAAATCCCTTTAAGTGCCTGTTGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTaa > 1:2550959/1‑69 (MQ=255)
tACCTGAAGAGAAATCCCTTTAAGTGCCTGTTGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTaa > 1:941322/1‑69 (MQ=255)
tACCTGAAGAGAAATCCCTTTAAGTGCCTGTTGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTaa > 1:895444/1‑69 (MQ=255)
tACCTGAAGAGAAATCCCTTTAAGTGCCTGTTGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTaa < 1:1737159/69‑1 (MQ=255)
tACCTGAAGAGAAATCCCTTTAAGTGCCTGTTGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTaa < 1:61750/69‑1 (MQ=255)
tACCTGAAGAGAAATCCCTTTAAGTGCCTGTTGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTaa > 1:3124747/1‑69 (MQ=255)
tACCTGAAGAGAAATCCCTTTAAGTGCCTGTTGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTaa > 1:3309389/1‑69 (MQ=255)
tACCTGAAGAGAAATCCCTTTAAGTGCCTGTTGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTaa < 1:3265829/69‑1 (MQ=255)
agaAATCCCTTTAAGTGCCTGTTGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGa < 1:3238394/69‑1 (MQ=255)
|
AGTGTTGATTTGCCGCATCCGGTTCGCCCGAGAATCGCTATATGTTCCCCGGCGTTTACCTGAAGAGAAATCCCTTTAAGTGCCTGTTGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGA > minE/584750‑584883
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
