Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
584,875 |
C→T |
37.0% |
T340T (ACG→ACA) |
cydC ← |
fused cysteine transporter subunits and membrane component and ATP‑binding component of ABC superfamily |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 584,875 | 0 | C | T | 37.0%
| 75.1
/ 71.6
| 116 | T340T (ACG→ACA) | cydC | fused cysteine transporter subunits and membrane component and ATP‑binding component of ABC superfamily |
| Reads supporting (aligned to +/- strand): ref base C (46/27); new base T (33/10); total (79/37) |
| Fisher's exact test for biased strand distribution p-value = 1.51e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
Only 100 of 116 total aligned reads displayed.
AGAAATCCCTTTAAGTGCCTGTTGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGTTTTTGATCC > minE/584815‑584941
|
agaAATCCCTTTAAGTGCCTGTTGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGa < 1:3238394/69‑1 (MQ=255)
aTCCCTTTAAGTGCCTGTTGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAAc > 1:3199331/1‑68 (MQ=255)
aTCCCTTTAAGTGCCTGTTGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAAc > 1:1857169/1‑68 (MQ=255)
aTCCCTTTAAGTGCCTGTTGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAAc > 1:493412/1‑68 (MQ=255)
tGCCTGTTGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAac > 1:3274249/1‑69 (MQ=255)
tGCCTGTTGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAac > 1:222156/1‑69 (MQ=255)
tGCCTGTTGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAac > 1:1796532/1‑69 (MQ=255)
ccTGTTGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAac > 1:2924009/1‑67 (MQ=255)
ccTGTTGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAac > 1:1989579/1‑67 (MQ=255)
ccTGTTGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAac > 1:1768354/1‑67 (MQ=255)
ccTGTTGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAac > 1:2392354/1‑67 (MQ=255)
ccTGTTGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAac > 1:1631836/1‑67 (MQ=255)
ccTGTTGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAac > 1:1431432/1‑67 (MQ=255)
ccTGTTGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAac > 1:1108280/1‑67 (MQ=255)
ccTGTTGAGATTGCTCCGGATAAGTGAACTGAACATCCCGAAACGTCAGCGAAACGCGATCGGCAac > 1:3084928/1‑67 (MQ=255)
gTTGGGATTGCTCCGGATAAGTGAACTGAACATCCCGTAATGTCAGCGAAACGCGATCGGCaa > 1:2456968/1‑63 (MQ=255)
gTTGGGATTGCTCCGGATAAGTGAACTGAACATCCCGTAATGTCAGCGAAACGCGATCGGCaa > 1:2102815/1‑63 (MQ=255)
gTTGGGATTGCTCCGGATAAGTGAACTGAACATCCCGTAATGTCAGCGAAACGCGATCGGCaa > 1:138500/1‑63 (MQ=255)
gTTGGGATTGCTCCGGATAAGTGAACTGAACATCCCGTAATGTCAGCGAAACGCGATCGGCaa > 1:2396272/1‑63 (MQ=255)
gTTGGGATTGCTCCGGATAAGTGAACTGAACATCCCGTAATGTCAGCGAAACGCGATCGGCaa > 1:408028/1‑63 (MQ=255)
gTTGGGATTGCTCCGGATAAGTGAACTGAACATCCCGTAATGTCAGCGAAACGCGATCGGCaa > 1:1411340/1‑63 (MQ=255)
gTTGGGATTGCTCCGGATAAGTGAACTGAACATCCCGTAATGTCAGCGAAACGCGATCGGCaa > 1:1071878/1‑63 (MQ=255)
gTTGGGATTGCTCCGGATAAGTGAACTGAACATCCCGTAATGTCAGCGAAACGCGATCGGCaa > 1:1551348/1‑63 (MQ=255)
gTTGGGATTGCTCCGGATAAGTGAACTGAACATCCCGTAATGTCAGCGAAACGCGATCGGCaa > 1:487865/1‑63 (MQ=255)
gTTGGGATTGCTCCGGATAAGTGAACTGAACATCCCGTAATGTCAGCGAAACGCGATCGGCaa > 1:526145/1‑63 (MQ=255)
gTTGGGATTGCTCCGGATAAGTGAACTGAACATCCCGTAATGTCAGCGAAACGCGATCGGCaa > 1:2696670/1‑63 (MQ=255)
gTTGGGATTGCTCCGGATAAGTGAACTGAACATCCCGTAATGTCAGCGAAACGCGATCGGCaa > 1:586930/1‑63 (MQ=255)
gTTGGGATTGCTCCGGATAAGTGAACTGAACATCCCGTAATGTCAGCGAAACGCGATCGGCa > 1:1954972/1‑62 (MQ=255)
gTTGGGATTGCTCCGGATAAGTGAACTGAACATCCCGTAATGTCAGCGAAACGCGATAGGCaa > 1:3117574/1‑63 (MQ=255)
ttGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGc > 1:1841536/1‑60 (MQ=255)
ttGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGc > 1:3009131/1‑60 (MQ=255)
ttGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGc < 1:2069367/60‑1 (MQ=255)
ttGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGc < 1:3140723/60‑1 (MQ=255)
ttGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGc < 1:2776107/60‑1 (MQ=255)
ttGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGc < 1:2761244/60‑1 (MQ=255)
ttGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGc > 1:3111685/1‑60 (MQ=255)
ttGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGc < 1:3127842/60‑1 (MQ=255)
ttGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGc < 1:1013300/60‑1 (MQ=255)
ttGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGc > 1:2745525/1‑60 (MQ=255)
ttGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGc > 1:1610876/1‑60 (MQ=255)
ttGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGc > 1:101955/1‑60 (MQ=255)
ttGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGc < 1:714001/60‑1 (MQ=255)
ttGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGc > 1:1505981/1‑60 (MQ=255)
ttGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGc > 1:1521637/1‑60 (MQ=255)
ttGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGc < 1:3307128/60‑1 (MQ=255)
ttGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGc > 1:1643479/1‑60 (MQ=255)
ttGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGc > 1:1741924/1‑60 (MQ=255)
ttGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGc < 1:355390/60‑1 (MQ=255)
ttGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGc < 1:351394/60‑1 (MQ=255)
ttGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGc > 1:336184/1‑60 (MQ=255)
ttGAGATTGCTACGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGc < 1:808642/60‑1 (MQ=255)
ttGAGATTGCTACGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGc > 1:826112/1‑60 (MQ=255)
gaTTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTg < 1:423728/68‑1 (MQ=255)
gaTTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTg < 1:2689102/68‑1 (MQ=255)
gaTTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTg < 1:2103499/68‑1 (MQ=255)
gaTTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTg < 1:118111/68‑1 (MQ=255)
tGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTggg > 1:1587722/1‑67 (MQ=255)
tGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGt > 1:2182085/1‑68 (MQ=255)
tGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTa < 1:2659788/69‑1 (MQ=255)
tGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTa < 1:898927/69‑1 (MQ=255)
tGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTa < 1:2424278/69‑1 (MQ=255)
gCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTggg > 1:2128173/1‑66 (MQ=255)
gCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGt > 1:1673613/1‑67 (MQ=255)
gATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAgg < 1:696161/67‑1 (MQ=255)
aTAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGaaa < 1:1195908/69‑1 (MQ=255)
tgaactgaacATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTg < 1:1435148/68‑1 (MQ=255)
tgaactgaacATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTg < 1:498568/68‑1 (MQ=255)
gaactgaacATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAg > 1:411625/1‑47 (MQ=255)
gaactgaacATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAgg > 1:994362/1‑60 (MQ=255)
gaactgaacATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGAc > 1:2004978/1‑69 (MQ=255)
gaactgaacATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGAc > 1:1950797/1‑69 (MQ=255)
gaactgaacATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGAc > 1:645326/1‑69 (MQ=255)
gaactgaacATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGAc > 1:1695749/1‑69 (MQ=255)
cATCCCGTAATGTCTGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCgg < 1:2027247/67‑1 (MQ=255)
cATCCCGTAATGTCTGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCgg < 1:2894927/67‑1 (MQ=255)
cATCCCGTAATGTCTGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCgg < 1:944109/67‑1 (MQ=255)
cATCCCGTAATGTCTGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCgg < 1:1220645/67‑1 (MQ=255)
cATCCCGTAATGTCTGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCgg < 1:2868622/67‑1 (MQ=255)
cATCCCGTAATGTCTGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCgg < 1:3039935/67‑1 (MQ=255)
cATCCCGTAATGTCTGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCgg < 1:2854218/67‑1 (MQ=255)
cATCCCGTAATGTCTGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCgg < 1:2058359/67‑1 (MQ=255)
cATCCCGTAATGTCTGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCgg < 1:419817/67‑1 (MQ=255)
cATCCCGTAATGTCTGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCgg < 1:2759708/67‑1 (MQ=255)
gTAATGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCGGttt > 1:1285841/1‑64 (MQ=255)
gTAATGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCGGttt > 1:976029/1‑64 (MQ=255)
gTAATGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCGGttt > 1:1987394/1‑64 (MQ=255)
gTAATGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCGGttt > 1:2878521/1‑64 (MQ=255)
gTAATGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCGGttt > 1:77616/1‑64 (MQ=255)
gTAATGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCGGttt > 1:176744/1‑64 (MQ=255)
gTAATGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCGGttt > 1:1525206/1‑64 (MQ=255)
gTAATGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCGGttt > 1:152905/1‑64 (MQ=255)
gTAATGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCGGttt > 1:49244/1‑64 (MQ=255)
gTAATGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCGGttt > 1:427320/1‑64 (MQ=255)
gTAATGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCGGtt > 1:1203064/1‑63 (MQ=255)
gTAATGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCGGtt > 1:1532091/1‑63 (MQ=255)
gTAATGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCGGTTTTTGag > 1:859107/1‑68 (MQ=255)
gTAATGTCAGCGAAACGCGATAGGCAACACGAGTTTGGGTATCAGGGAAGGTGACTTCCGGttt > 1:2467483/1‑64 (MQ=255)
aaCGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGTTTTTGATcc > 1:1825163/1‑69 (MQ=255)
aaCGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGTTTTTGATcc > 1:334420/1‑69 (MQ=255)
aaCGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGTTTTTGATcc > 1:931577/1‑69 (MQ=255)
|
AGAAATCCCTTTAAGTGCCTGTTGAGATTGCTCCGGATAAGTGAACTGAACATCCCGTAACGTCAGCGAAACGCGATCGGCAACACGAGTTTGGGTATCAGGAAAGGTGACCTCCGGTTTTTGATCC > minE/584815‑584941
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
