Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
586,692 |
G→A |
59.2% |
L324L (CTG→TTG) |
cydD ← |
fused cysteine transporter subunits and membrane component and ATP‑binding component of ABC superfamily |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 586,692 | 0 | G | A | 59.2%
| 25.7
/ 49.9
| 71 | L324L (CTG→TTG) | cydD | fused cysteine transporter subunits and membrane component and ATP‑binding component of ABC superfamily |
| Reads supporting (aligned to +/- strand): ref base G (17/12); new base A (18/24); total (35/36) |
| Fisher's exact test for biased strand distribution p-value = 2.32e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CGATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAGACTGTCAGCTGCGCCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATGGC > minE/586630‑586756
|
cGATGCTAATTCCGCCTCACCACGTTGCGGATGTGCGAGCGGGGTTTCCATAAACGTTTTCAGACTGTc < 1:470628/69‑1 (MQ=255)
cGATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAGACTGTc < 1:2168189/69‑1 (MQ=255)
cGATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAGACTGTc < 1:1553081/69‑1 (MQ=255)
gCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAGACTGTCAGc > 1:361501/1‑68 (MQ=255)
gCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAGACTGTCAGCt > 1:1066927/1‑69 (MQ=255)
ccGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAGACTGTCAGCTGCGcc > 1:981469/1‑67 (MQ=255)
ccGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAGACTGTCAGCTGCGcc > 1:2949387/1‑67 (MQ=255)
ccGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAGACTGTCAGCTGCGcc > 1:2230740/1‑67 (MQ=255)
ccGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAGACTGTCAGCTGCGcc > 1:1357107/1‑67 (MQ=255)
ccGCCTCACCACGTTG‑GGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAGACTGTCAGCTGCGCCAAc > 1:2304959/1‑69 (MQ=255)
tCACCACGTTGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAGACTGTc < 1:2604136/53‑1 (MQ=255)
aaCGTTTTCAGACTGTCAGCTGCGCCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGAt > 1:1492334/1‑64 (MQ=255)
aaCGTTTTCAGACTGTCAGCTGCGCCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGt > 1:2854522/1‑69 (MQ=255)
aaCGTTTTCAGACTGTCAGCTGCGCCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGt > 1:1071013/1‑69 (MQ=255)
aaCGTTTTCAGACTGTCAGCTGCGCCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGt > 1:891135/1‑69 (MQ=255)
aaCGTTTTCAGACTGTCAGCTGCGCCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGt > 1:577131/1‑69 (MQ=255)
aaCGTTTTCAGACTGTCAGCTGCGCCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGt > 1:521668/1‑69 (MQ=255)
aaCGTTTTCAGACTGTCAGCTGCGCCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGt < 1:1123559/69‑1 (MQ=255)
aaCGTTTTCAGACTGTCAGCTGCGCCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGt < 1:346822/69‑1 (MQ=255)
aaCGTTTTCAGACTGTCAGCTGCGCCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGt > 1:3319821/1‑69 (MQ=255)
aaCGTTTTCAGACTGTCAGCTGCGCCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGt < 1:3231661/69‑1 (MQ=255)
aaCGTTTTCAGACTGTCAGCTGCGCCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGt > 1:3118006/1‑69 (MQ=255)
aaCGTTTTCAGACTGTCAGCTGCGCCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGt < 1:30083/69‑1 (MQ=255)
aaCGTTTTCAGACTGTCAGCTGCGCCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGt > 1:298850/1‑69 (MQ=255)
aaCGTTTTCAGACTGTCAGCTGCGCCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGt < 1:1127607/69‑1 (MQ=255)
aaCGTTTTCAGACTGTCAGCTGCGCCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGt < 1:2608468/69‑1 (MQ=255)
aaCGTTTTCAGACTGTCAGCTGCGCCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGt > 1:2050419/1‑69 (MQ=255)
aaCGTTTTCAGACTGTCAGCTGCGCCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGt < 1:2014008/69‑1 (MQ=255)
aaCGTTTTCAGACTGTCAGCTGCGCCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGt < 1:1647431/69‑1 (MQ=255)
ttttCAAACTGTCAGCTGCACCAACAGCCTGGGCTTTAGCATGATAAAACGTACCTAGATCGCGTAATg < 1:3191617/69‑1 (MQ=255)
ttttCAAACTGTCAGCTGCACCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATg < 1:331982/69‑1 (MQ=255)
ttttCAAACTGTCAGCTGCACCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATg < 1:2958824/69‑1 (MQ=255)
ttttCAAACTGTCAGCTGCACCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATg < 1:2990887/69‑1 (MQ=255)
ttttCAAACTGTCAGCTGCACCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATg < 1:3000196/69‑1 (MQ=255)
ttttCAAACTGTCAGCTGCACCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATg < 1:30899/69‑1 (MQ=255)
ttttCAAACTGTCAGCTGCACCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATg < 1:1786315/69‑1 (MQ=255)
ttttCAAACTGTCAGCTGCACCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATg < 1:1294858/69‑1 (MQ=255)
ttttCAAACTGTCAGCTGCACCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATg < 1:3383940/69‑1 (MQ=255)
ttttCAAACTGTCAGCTGCACCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATg < 1:1674325/69‑1 (MQ=255)
ttttCAAACTGTCAGCTGCACCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATg < 1:1081647/69‑1 (MQ=255)
ttttCAAACTGTCAGCTGCACCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATg < 1:1528427/69‑1 (MQ=255)
ttttCAAACTGTCAGCTGCACCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATg < 1:641896/69‑1 (MQ=255)
ttttCAAACTGTCAGCTGCACCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATg < 1:681730/69‑1 (MQ=255)
ttttCAAACTGTCAGCTGCACCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATg < 1:842066/69‑1 (MQ=255)
ttttCAAACTGTCAGCTGCACCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATg < 1:121523/69‑1 (MQ=255)
ttttCAAACTGTCAGCTGCACCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATg < 1:2553191/69‑1 (MQ=255)
ttttCAAACTGTCAGCTGCACCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATg < 1:2488531/69‑1 (MQ=255)
ttttCAAACTGTCAGCTGCACCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATg < 1:2367632/69‑1 (MQ=255)
ttttCAAACTGTCAGCTGCACCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATg < 1:2685717/69‑1 (MQ=255)
ttttCAAACTGTCAGCTGCACCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATg < 1:2222194/69‑1 (MQ=255)
ttttCAAACTGTCAGCTGCACCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATg < 1:2754871/69‑1 (MQ=255)
ttttCAAACTGTCAGCTGCACCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATg < 1:1416464/69‑1 (MQ=255)
ttttCAAACTGTCAGCTGCACCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATg < 1:2940688/69‑1 (MQ=255)
tCAAACTGTCAGCTGCACCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATgg > 1:2214079/1‑67 (MQ=255)
tCAAACTGTCAGCTGCACCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATg > 1:2395320/1‑66 (MQ=255)
tCAAACTGTCAGCTGCACCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATGGc > 1:1514330/1‑68 (MQ=255)
tCAAACTGTCAGCTGCACCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATGGc > 1:1429158/1‑68 (MQ=255)
tCAAACTGTCAGCTGCACCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATGGc > 1:970383/1‑68 (MQ=255)
tCAAACTGTCAGCTGCACCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATGGc > 1:1384445/1‑68 (MQ=255)
tCAAACTGTCAGCTGCACCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATGGc > 1:2483380/1‑68 (MQ=255)
tCAAACTGTCAGCTGCACCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATGGc > 1:1342572/1‑68 (MQ=255)
tCAAACTGTCAGCTGCACCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATGGc > 1:256210/1‑68 (MQ=255)
tCAAACTGTCAGCTGCACCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATGGc > 1:1744073/1‑68 (MQ=255)
tCAAACTGTCAGCTGCACCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATGGc > 1:1192614/1‑68 (MQ=255)
tCAAACTGTCAGCTGCACCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATGGc > 1:3308650/1‑68 (MQ=255)
tCAAACTGTCAGCTGCACCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATGGc > 1:3202086/1‑68 (MQ=255)
tCAAACTGTCAGCTGCACCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATGGc > 1:3150955/1‑68 (MQ=255)
tCAAACTGTCAGCTGCACCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATGGc > 1:1886626/1‑68 (MQ=255)
tCAAACTGTCAGCTGCACCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATGGc > 1:2751521/1‑68 (MQ=255)
tCAAACTGTCAGCTGCACCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATGGc > 1:2814456/1‑68 (MQ=255)
tCAAACTGTCAGCTGCACCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATGGc > 1:2908742/1‑68 (MQ=255)
|
CGATGCTAATTCCGCCTCACCACGTTGCGGATGGGCGAGCGGGGTTTCCATAAACGTTTTCAGACTGTCAGCTGCGCCAACAGCCTGGGCTTTAGCATGATAAAACGTACCGAGATCGCGTAATGGC > minE/586630‑586756
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
