Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
608,544 |
A→G |
29.7% |
N570N (AAT→AAC) |
pflB ← |
pyruvate formate lyase I |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 608,544 | 0 | A | G | 29.7%
| 106.9
/ 54.1
| 114 | N570N (AAT→AAC) | pflB | pyruvate formate lyase I |
| Reads supporting (aligned to +/- strand): ref base A (52/28); new base G (12/22); total (64/50) |
| Fisher's exact test for biased strand distribution p-value = 4.15e-03 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.88e-01 |
Only 100 of 114 total aligned reads displayed.
CAGTTTCTGAATTTTCTTCATGAAACGTTCTACCAGGTCAACAGCCAGGTCATCTACACGCGGATCATTGTTACCAAACTGCGGGTATTCGCCTTCGATTTCGAAGTCGATAGCCAGACCGTCTTCGTC > minE/608478‑608606
|
cAGTTTCTGAATTTTCTTCATGAAACGTTCTACCAGGTCAACAGCCAGGTCATCTACACGCGGATCAtt < 1:2014258/69‑1 (MQ=255)
cAGTTTCTGAATTTTCTTCATGAAACGTTCTACCAGGTCAACAGCCAGGTCATCTACACGCGGATCAtt > 1:2459678/1‑69 (MQ=255)
cAGTTTCTGAATTTTCTTCATGAAACGTTCTACCAGGTCAACAGCCAGGTCATCTACACGCGGATCAtt > 1:2508690/1‑69 (MQ=255)
cAGTTTCTGAATTTTCTTCATGAAACGTTCTACCAGGTCAACAGCCAGGTCATCTACACGCGGATCAtt < 1:1455261/69‑1 (MQ=255)
cAGTTTCTGAATTTTCTTCATGAAACGTTCTACCAGGTCAACAGCCAGGTCATCTACACGCGGATCAtt > 1:1417187/1‑69 (MQ=255)
cAGTTTCTGAATTTTCTTCATGAAACGTTCTACCAGGTCAACAGCCAGGTCATCTACACGCGGATCAtt > 1:2715336/1‑69 (MQ=255)
cAGTTTCTGAATTTTCTTCATGAAACGTTCTACCAGGTCAACAGCCAGGTCATCTACACGCGGATCAtt < 1:1667174/69‑1 (MQ=255)
cAGTTTCTGAATTTTCTTCATGAAACGTTCTACCAGGTCAACAGCCAGGTCATCTACACGCGGATCAtt < 1:167103/69‑1 (MQ=255)
cAGTTTCTGAATTTTCTTCATGAAACGTTCTACCAGGTCAACAGCCAGGTCATCTACACGCGGATCAtt < 1:2306635/69‑1 (MQ=255)
cAGTTTCTGAATTTTCTTCATGAAACGTTCTACCAGGTCAACAGCCAGGTCATCTACACGCGGATCAtt < 1:3110821/69‑1 (MQ=255)
cAGTTTCTGAATTTTCTTCATGAAACGTTCTACCAGGTCAACAGCCAGGTCATCTACACGCGGATCAtt > 1:1009625/1‑69 (MQ=255)
cAGTTTCTGAATTTTCTTCATGAAACGTTCTACCAGGTCAACAGCCAGGTCATCTACACGCGGATCAtt > 1:1276067/1‑69 (MQ=255)
cAGTTTCTGAATTTTCTTCATGAAACGTTCTACCAGGTCAACAGCCAGGTCATCTACACGCGGATCAtt > 1:474430/1‑69 (MQ=255)
cAGTTTCTGAATTTTCTTCATGAAACGTTCTACCAGGTCAACAGCCAGGTCATCTACACGCGGATCAtt > 1:478428/1‑69 (MQ=255)
cAGTTTCTGAATTTTCTTCATGAAACGTTCTACCAGGTCAACAGCCAGGTCATCTACACGCGGATCAtt > 1:111156/1‑69 (MQ=255)
cAGTTTCTGAATTTTCTTCATGAAACGTTCTACCAGGTCAACAGCCAGGTCATCTACACGCGGATCAtt < 1:1965662/69‑1 (MQ=255)
cAGTTTCTGAATTTTCTTCATGAAACGTTCTACCAGGTCAACAGCCAGGTCATCTACACGCGGATCAtt < 1:1063826/69‑1 (MQ=255)
aGTTTCTGAATTTTCTTCATGAAACGTTCTACCAGGTCAACAGCCAGGTCATCTACACGCGGATCAtt < 1:1105992/68‑1 (MQ=255)
aaTTTTCTTCATGAAACGTTCTACCAGGTCAACAGCCAGGTCATCTACACGCGGATCAtt < 1:2851716/60‑1 (MQ=255)
ttttCTTCATGAAACGTTCTACCAGGTCAACAGCCAGGTCATCTACACGCGGATCATTGTTACCaa < 1:2268814/66‑1 (MQ=255)
ttttCTTCATGAAACGTTCTACCAGGTCAACAGCCAGGTCATCTACACGCGGATCATTGTTACCaa < 1:3384846/66‑1 (MQ=255)
tcATGAAACGTTCTACCAGGTCAACAGCCAGGTCATCTACACGCGGATCATTGTTACCAAACTGCggg < 1:2246568/68‑1 (MQ=255)
tcATGAAACGTTCTACCAGGTCAACAGCCAGGTCATCTACACGCGGATCATTGTTACCAAACTGCggg < 1:1350150/68‑1 (MQ=255)
tcATGAAACGTTCTACCAGGTCAACAGCCAGGTCATCTACACGCGGATCATTGTTACCAAACTGCggg < 1:2734489/68‑1 (MQ=255)
tcATGAAACGTTCTACCAGGTCAACAGCCAGGTCATCTACACGCGGATCATTGTTACCAAACTGCggg < 1:2183986/68‑1 (MQ=255)
tcATGAAACGTTCTACCAGGTCAACAGCCAGGTCATCTACACGCGGATCATTGTTACCAAACTGCggg < 1:2447876/68‑1 (MQ=255)
tcATGAAACGTTCTACCAGGTCAACAGCCAGGTCATCTACACGCGGATAATTGTTACCAAACTGCggg < 1:3112824/68‑1 (MQ=255)
gAAACGTTCTACCAGGTCAACAGCCAGGTCATCTACACGCGGATCATTGTTACCAAACTGCGGGTATTc < 1:210667/69‑1 (MQ=255)
aaaCGTTCTACCAGGTCAACAGCCAGGTCATCTACACGCGGATCATTGTTACCAAACTGCGGGTATTCg < 1:2838270/69‑1 (MQ=255)
aaaCGTTCTACCAGGTCAACAGCCAGGTCATCTACACGCGGATCATTGTTACCAAACTGCGGGTATTCg < 1:3279252/69‑1 (MQ=255)
aaaCGTTCTACCAGGTCAACAGCCAGGTCATCTACACGCGGATCATTGTTACCAAACTGCGGGTATTCg < 1:1204878/69‑1 (MQ=255)
aaCGTTCTACCAGGTCAACAGCGAGGTCATCTACACGCGGATCGTTGTTACCAAACTg < 1:1293408/58‑1 (MQ=255)
aaCGTTCTACCAGGTCAACAGCCAGGTCATCTACACGCGGATCGTTGTTACCAAACTg < 1:264832/58‑1 (MQ=255)
aaCGTTCTACCAGGTCAACAGCCAGGTCATCTACACGCGGATCGTTGTTACCAAACTg < 1:2345610/58‑1 (MQ=255)
aaCGTTCTACCAGGTCAACAGCCAGGTCATCTACACGCGGATCGTTGTTACCAAACTg < 1:2882597/58‑1 (MQ=255)
aaCGTTCTACCAGGTCAACAGCCAGGTCATCTACACGCGGATCGTTGTTACCAAACTg < 1:2968811/58‑1 (MQ=255)
aaCGTTCTACCAGGTCAACAGCCAGGTCATCTACACGCGGATCGTTGTTACCAAACTg < 1:602135/58‑1 (MQ=255)
aaCGTTCTACCAGGTCAACAGCCAGGTCATCTACACGCGGATCGTTGTTACCAAACTg < 1:946365/58‑1 (MQ=255)
aaCGTTCTACCAGGTCAACAGCCAGGTCATCTACACGCGGATCGTTGTTACCAAACTg < 1:946635/58‑1 (MQ=255)
aaCGTTCTACCAGGTCAACAGCCAGGTCATCTACACGCGGATCGTTGTTACCAAACTg < 1:2301049/58‑1 (MQ=255)
aaCGTTCTACCAGGTCAACAGCCAGGTCATCTACACGCGGATCGTTGTTACCAAACTg < 1:1333704/58‑1 (MQ=255)
aaCGTTCTACCAGGTCAACAGCCAGGTCATCTACACGCGGATCGTTGTTACCAAACTg < 1:1099815/58‑1 (MQ=255)
aaCGTTCTACCAGGTCAACAGCCAGGTCATCTACACGCGGATCGTTGTTACCAAACTg < 1:1338779/58‑1 (MQ=255)
aaCGTTCTACCAGGTCAACAGCCAGGTCATCTACACGCGGATCGTTGTTACCAAACTg < 1:1553543/58‑1 (MQ=255)
aaCGTTCTACCAGGTCAACAGCCAGGTCATCTACACGCGGATCGTTGTTACCAAACTg < 1:1665578/58‑1 (MQ=255)
aaCGTTCTACCAGGTCAACAGCCAGGTCATCTACACGCGGATCGTTGTTACCAAACTg < 1:1745741/58‑1 (MQ=255)
aaCGTTCTACCAGGTCAACAGCCAGGTCATCTAAACGCGGATCGTTGTTACCAAACTg < 1:3045952/58‑1 (MQ=255)
ccAGGTCAACAGCCAGGTCATCTACACGCGGATCGTTGTTACCAAACTGCGGGTATTCGCCTTCGAtt > 1:595690/1‑68 (MQ=255)
ccAGGTCAACAGCCAGGTCATCTACACGCGGATCGTTGTTACCAAACTGCGGGTATTCGCCTTCGAtt > 1:1180807/1‑68 (MQ=255)
ccAGGTCAACAGCCAGGTCATCTACACGCGGATCGTTGTTACCAAACTGCGGGTATTCGCCTTCGAtt > 1:1511191/1‑68 (MQ=255)
ccAGGTCAACAGCCAGGTCATCTACACGCGGATCGTTGTTACCAAACTGCGGGTATTCGCCTTCGAtt > 1:2378103/1‑68 (MQ=255)
ccAGGTCAACAGCCAGGTCATCTACACGCGGATCGTTGTTACCAAACTGCGGGTATTCGCCTTCGAtt > 1:2725140/1‑68 (MQ=255)
ccAGGTCAACAGCCAGGTCATCTACACGCGGATCGTTGTTACCAAACTGCGGGTATTCGCCTTCGAtt > 1:1347381/1‑68 (MQ=255)
ccAGGTCAACAGCCAGGTCATCTACACGCGGATCGTTGTTACCAAACTGCGGGTATTCGCCTTCGAtt > 1:71791/1‑68 (MQ=255)
ccAGGTCAACAGCCAGGTCATCTACACGCGGATCGTTGTTACCAAACTGCGGGTATTCGCCTTCGAtt > 1:186214/1‑68 (MQ=255)
ggTCAAGAGCCAGGTCATCTACACGCGGATCATTGTTACCaa < 1:665456/42‑1 (MQ=255)
ggTCAACAGCCAGGTCATCTACACGCGGATCGTTGTTACCAAACTg < 1:610730/46‑1 (MQ=255)
tCAACAGCCAGGTCATCTACACGCGGATCGTTGTTACCAAACTg < 1:1110268/44‑1 (MQ=255)
gTCATCTACACGCGGATCATTGTTACCAAACTGCGGGTATTct < 1:2864401/43‑2 (MQ=255)
tCATCTACACGCGGATCATTGTTACCAAACTGCGGGTATTCGCCTTCGATTTCGAAGTCGATAGCCAg > 1:2093414/1‑68 (MQ=255)
tCATCTACACGCGGATCATTGTTACCAAACTGCGGGTATTCGCCTTCGATTTCGAAGTCGATAGCCAGa > 1:1346818/1‑69 (MQ=255)
tCATCTACACGCGGATCATTGTTACCAAACTGCGGGTATTCGCCTTCGATTTCGAAGTCGATAGCCAGa > 1:2190554/1‑69 (MQ=255)
tCATCTACACGCGGATCATTGTTACCAAACTGCGGGTATTCGCCTTCGATTTCGAAGTCGATAGCCAGa > 1:3024079/1‑69 (MQ=255)
cATCTACACGCGGATCATTGTTACCAAACTGCGGGTATTCGCCTTCGATTTCGAAGTCGATAGCCAGAc > 1:2615682/1‑69 (MQ=255)
cATCTACACGCGGATCATTGTTACCAAACTGCGGGTATTCGCCTTCGATTTCGAAGTCGATAGCCAGAc > 1:2635401/1‑69 (MQ=255)
cATCTACACGCGGATCATTGTTACCAAACTGCGGGTATTCGCCTTCGATTTCGAAGTCGATAGCCAGAc > 1:2700367/1‑69 (MQ=255)
cATCTACACGCGGATCATTGTTACCAAACTGCGGGTATTCGCCTTCGATTTCGAAGTCGATAGCCAGAc > 1:3184173/1‑69 (MQ=255)
cATCTACACGCGGATCATTGTTACCAAACTGCGGGTATTCGCCTTCGATTTCGAAGTCGATAGCCAGAc > 1:1749389/1‑69 (MQ=255)
cATCTACACGCGGATCATTGTTACCAAACTGCGGGTATTCGCCTTCGATTTCGAAGTCGATAGCCAGAc > 1:129650/1‑69 (MQ=255)
cATCTACACGCGGATCATTGTTACCAAACTGCGGGTATTCGCCTTCGATTTCGAAGTCGATAGCCAGAc > 1:1778549/1‑69 (MQ=255)
cATCTACACGCGGATCATTGTTACCAAACTGCGGGTATTCGCCTTCGATTTCGAAGTCGATAGCCAGAc > 1:2878347/1‑69 (MQ=255)
cATCTACACGCGGATCATTGTTACCAAACTGCGGGTATTCGCCTTCGATTTCGAAGTCGATAGCCAGAc > 1:2254911/1‑69 (MQ=255)
tACCCGCGGATCATTGTTACCAAACTGCGGGTATTCGCCTTCGATTTCGAAGTCGATAGCCAGACCGTc > 1:10318/1‑69 (MQ=255)
tACACGCGGATCATTGTTACCCAACTGCGGGTATTCGCCTTCGATTTCGAAGTCGATAGCCAGACCGTc > 1:83951/1‑69 (MQ=255)
tACACGCGGATCATTGTTACCAAACTGCGGGTATTCGCCTTCGATTTCGAAGTCGAt < 1:2945173/57‑1 (MQ=255)
tACACGCGGATCATTGTTACCAAACTGCGGGTATTCGCCTTCGATTTCGAAGTCGAt < 1:1135254/57‑1 (MQ=255)
tACACGCGGATCATTGTTACCAAACTGCGGGTATTCGCCTTCGATTTCGAAGTCGAt < 1:2213239/57‑1 (MQ=255)
tACACGCGGATCATTGTTACCAAACTGCGGGTATTCGCCTTCGATTTCGAAGTCGATAGCCAGACCGt > 1:1782883/1‑68 (MQ=255)
tACACGCGGATCATTGTTACCAAACTGCGGGTATTCGCCTTCGATTTCGAAGTCGATAGCCAGACCGt > 1:1406598/1‑68 (MQ=255)
tACACGCGGATCATTGTTACCAAACTGCGGGTATTCGCCTTCGATTTCGAAGTCGATAGCCAGACCGTc > 1:1360244/1‑69 (MQ=255)
tACACGCGGATCATTGTTACCAAACTGCGGGTATTCGCCTTCGATTTCGAAGTCGATAGCCAGACCGTc > 1:3214488/1‑69 (MQ=255)
tACACGCGGATCATTGTTACCAAACTGCGGGTATTCGCCTTCGATTTCGAAGTCGATAGCCAGACCGTc > 1:1332712/1‑69 (MQ=255)
tACACGCGGATCATTGTTACCAAACTGCGGGTATTCGCCTTCGATTTCGAAGTCGATAGCCAGACCGTc > 1:331618/1‑69 (MQ=255)
tACACGCGGATCATTGTTACCAAACTGCGGGTATTCGCCTTCGATTTCGAAGTCGATAGCCAGACCGTc > 1:3344154/1‑69 (MQ=255)
tACACGCGGATCATTGTTACCAAACTGCGGGTATTCGCCTTCGATTTCGAAGTCGATAGCCAGACCGTc > 1:857367/1‑69 (MQ=255)
tACACGCGGATCATTGTTACCAAACTGCGGGTATTCGCCTTCGATTTCGAAGTCGATAGCCAGACCGTc > 1:640715/1‑69 (MQ=255)
tACACGCGGATCATTGTTACCAAACTGCGGGTATTCGCCTTCGATTTCGAAGTCGATAGCCAGACCGTc > 1:432584/1‑69 (MQ=255)
tACACGCGGATCATTGTTACCAAACTGCGGGTATTCGCCTTCGATTTCGAAGTCGATAGCCAGACCGTc > 1:14523/1‑69 (MQ=255)
tACACGCGGATCATTGTTACCAAACTGCGGGTATTCGCCTTCGATTTCGAAGTCGATAGCCAGACCGTc > 1:502438/1‑69 (MQ=255)
tACACGCGGATCATTGTTACCAAACTGCGGGTATTCGCCTTCGATTTCGAAGTCGATAGCCAGACCGTc > 1:1503088/1‑69 (MQ=255)
tACACGCGGATCATTGTTACCAAACTGCGGGTATTCGCCTTCGATTTCGAAGTCGATAGCCAGACCGTc > 1:2501698/1‑69 (MQ=255)
cacGCGGATCATTGTTACCAAACTGCGGGTATTCGCCTTCGATTTCGAAGTCGATAGCCAGACCGTCtt > 1:2119154/1‑69 (MQ=255)
cacGCGGATCATTGTTACCAAACTGCGGGTATTCGCCTTCGATTTCGAAGTCGATAGCCAGACCGTCtt > 1:2032682/1‑69 (MQ=255)
cacGCGGATCATTGTTACCAAACTGCGGGTATTCGCCTTCGATTTCGAAGTCGATAGCCAGACCGTCtt > 1:3385305/1‑69 (MQ=255)
cacGCGGATCATTGTTACCAAACTGCGGGTATTCGCCTTCGATTTCGAAGTCGATAGCCAGACCGTCtt > 1:1186610/1‑69 (MQ=255)
cacGCGGATCATTGTTACCAAACTGCGGGTATTCGCCTTCGATTTCGAAGTCGATAGCCAGACCGTCtt > 1:2881538/1‑69 (MQ=255)
cacGCGGATCATTGTTACCAAACTGCGGGTATTCGCCTTCGATTTCGAAGTCGATAGCCAGACCGTCtt > 1:2885512/1‑69 (MQ=255)
cacGCGGATCATTGTTACCAAACTGCGGGTATTCGCCTTCGATTTCGAAGTCGATAGCCAGACCGTCtt > 1:2920988/1‑69 (MQ=255)
aacGCGGATCATTGTTACCAAACTGCGGGTATTCGCCTTCGATTTCGAAGTCGATAGCCAGACCGTCtt > 1:1257355/2‑69 (MQ=255)
cgGATCATTGTTACCAAACTGCGGGTATTCGCCTTCGATTTCGAAGTCGATAGCCAGACCGTCTTCGTc > 1:2214093/1‑69 (MQ=255)
|
CAGTTTCTGAATTTTCTTCATGAAACGTTCTACCAGGTCAACAGCCAGGTCATCTACACGCGGATCATTGTTACCAAACTGCGGGTATTCGCCTTCGATTTCGAAGTCGATAGCCAGACCGTCTTCGTC > minE/608478‑608606
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
