Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F1 I0 R1	4577	57.4	3436498	77.7%	2670158	63.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	615,152	A→G	52.8%	K267K (AAA→AAG)	serC →	3‑phosphoserine/phosphohydroxythreonine aminotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	615,152	0	A	G	52.8%	9.2 / 77.8	91	K267K (AAA→AAG)	serC	3‑phosphoserine/phosphohydroxythreonine aminotransferase
Reads supporting (aligned to +/- strand): ref base A (9/34); new base G (21/27); total (30/61)
Fisher's exact test for biased strand distribution p-value = 2.62e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01

TATCTGGTCTGGTCTTTAAATGGCTGAAAGCGAACGGCGGTGTAGCTGAAATGGATAAAATCAATCAGCAAAAAGCAGAACTGCTATATGGGGTGATTGATAACAGCGATTTCTACCGCAATGA  >  minE/615094‑615217

tatctGGTCTGGTCTTTAAATGGCTGAAAGCGAACGGCGGTGTAGCTGAAATGGATAAAatcaatca                                                           >  1:2363037/1‑67 (MQ=255)

tatctGGTCTGGTCTTTAAATGGCTGAAAGCGAACGGCGGTGTAGCTGAAATGGATAAAATCAATCAg                                                          >  1:1719596/1‑68 (MQ=255)

tatctGGTCTGGTCTTTAAATGGCTGAAAGCGAACGGCGGTGTAGCTGAAATGGATAAAATCAATCAg                                                          >  1:2506641/1‑68 (MQ=255)

         tggtctTTAAATGGCTGAAAGCGAACGGCGGTGTAGCTGAAATGGATAAAATCAATCAGCAAAAAGCAg                                                <  1:613084/69‑1 (MQ=255)

         tggtctTTAAATGGCTGAAAGCGAACGGCGGTGTAGCTGAAATGGATAAAATCAATCAGCAAAAAGCAg                                                <  1:710005/69‑1 (MQ=255)

         tggtctTTAAATGGCTGAAAGCGAACGGCGGTGTAGCTGAAATGGATAAAATCAATCAGCAAAAAGCAg                                                <  1:2533610/69‑1 (MQ=255)

         tggtctTTAAATGGCTGAAAGCGAACGGCGGTGTAGCTGAAATGGATAAAATCAATCAGCAAAAAGCAg                                                <  1:3359934/69‑1 (MQ=255)

         tggtctTTAAATGGCTGAAAGCGAACGGCGGTGTAGCTGAAATGGATAAAATCAATCAGCAAAAAGCAg                                                <  1:1382052/69‑1 (MQ=255)

         tggtctTTAAATGGCTGAAAGCGAACGGCGGTGTAGCTGAAATGGATAAAATCAATCAGCAAAAAGCAg                                                <  1:2713493/69‑1 (MQ=255)

         tggtctTTAAATGGCTGAAAGCGAACGGCGGTGTAGCTGAAATGGATAAAATCAATCAGCAAAAAGCAg                                                <  1:2727206/69‑1 (MQ=255)

         tggtctTTAAATGGCTGAAAGCGAACGGCGGTGTAGCTGAAATGGATAAAATCAATCAGCAAAAAGCAg                                                <  1:1944513/69‑1 (MQ=255)

         tggtctTTAAATGGCTGAAAGCGAACGGCGGTGTAGCTGAAATGGATAAAATCAATCAGCAAAAAGCAg                                                <  1:1910901/69‑1 (MQ=255)

         tggtctTTAAATGGCTGAAAGCGAACGGCGGTGTAGCTGAAATGGATAAAATCAATCAGCAAAAAGCAg                                                <  1:2750113/69‑1 (MQ=255)

                  aaTGGCTGAAAGCGAACGGCGGTGTAGCTGAAATGGATAAAATCAATCAGCAAAAAGCAGAACTGCta                                        <  1:2585734/68‑1 (MQ=255)

                  aaTGGCTGAAAGCGAACGGCGGTGTAGCTGAAATGGATAAAATCAATCAGCAAAAAGCAGAACTGCta                                        <  1:2632714/68‑1 (MQ=255)

                  aaTGGCTGAAAGCGAACGGCGGTGTAGCTGAAATGGATAAAATCAATCAGCAAAAAGCAGAACTGCta                                        <  1:2027381/68‑1 (MQ=255)

                  aaTGGCTGAAAGCGAACGGCGGTGTAGCTGAAATGGATAAAATCAATCAGCAAAAAGCAGAACTGCta                                        <  1:2023596/68‑1 (MQ=255)

                  aaTGGCTGAAAGCGAACGGCGGTGTAGCTGAAATGGATAAAATCAATCAGCAAAAAGCAGAACTGCta                                        <  1:1692041/68‑1 (MQ=255)

                  aaTGGCTGAAAGCGAACGGCGGTGTAGCTGAAATGGATAAAATCAATCAGCAAAAAGCAGAACTGCta                                        <  1:1529405/68‑1 (MQ=255)

                  aaTGGCTGAAAGCGAACGGCGGTGTAGCTGAAATGGATAAAATCAATCAGCAAAAAGCAGAACTGCta                                        <  1:3202281/68‑1 (MQ=255)

                  aaTGGCTGAAAGCGAACGGCGGTGTAGCTGAAATGGATAAAATCAATCAGCAAAAAGCAGAACTGCta                                        <  1:3207136/68‑1 (MQ=255)

                  aaTGGCTGAAAGCGAACGGCGGTGTAGCTGAAATGGATAAAATCAATCAGCAAAAAGCAGAACTGCta                                        <  1:3279989/68‑1 (MQ=255)

                  aaTGGCTGAAAGCGAACGGCGGTGTAGCTGAAATGGATAAAATCAATCAGCAAAAAGCAGAACTGCta                                        <  1:3375483/68‑1 (MQ=255)

                  aaTGGCTGAAAGCGAACGGCGGTGTAGCTGAAATGGATAAAATCAATCAGCAAAAAGCAGAACTGCta                                        <  1:3394545/68‑1 (MQ=255)

                  aaTGGCTGAAAGAGAACGGCGGGGTAGCTGAAATGGATAAAATCAATCAGCAAAAAGCAGAACTGCta                                        <  1:315573/68‑1 (MQ=255)

                      gCTGAAAGCGAACGGCGGTGTAGCTGAAATGGATAAAATCAATCAGCAAAAAGCAGAACTGCTATATgg                                   >  1:2911288/1‑69 (MQ=255)

                      gCTGAAAGCGAACGGCGGTGTAGCTGAAATGGATAAAATCAATCAGCAAAAAGCAGAACTGCTATATgg                                   >  1:3078506/1‑69 (MQ=255)

                      gCTGAAAGCGAACGGCGGTGTAGCTGAAATGGATAAAATCAATCAGCAAAAAGCAGAACTGCTATATgg                                   >  1:1994033/1‑69 (MQ=255)

                      gCTGAAAGCGAACGGCGGTGTAGCTGAAATGGATAAAATCAATCAGCAAAAAGCAGAACTGCTATATgg                                   >  1:3144157/1‑69 (MQ=255)

                      gCTGAAAGCGAACGGCGGTGTAGCTGAAATGGATAAAATCAATCAGCAAAAAGCAGAACTGCTATATgg                                   >  1:1067910/1‑69 (MQ=255)

                         gAAAGCGAACGGCGGTGTAGCTGAAATGGATAAAATCAATCAGCAAAAAGCGGAACTGCTATATGGGGt                                <  1:2702281/69‑1 (MQ=255)

                         gAAAGCGAACGGCGGTGTAGCTGAAATGGATAAAATCAATCAGCAAAAAGCAGAACTGCTATATGGGGt                                <  1:2632136/69‑1 (MQ=255)

                         gAAAGCGAACGGCGGTGTAGCTGAAATGGATAAAATCAATCAGCAAAAAGCAGAACTGCTATATGGGGt                                <  1:297780/69‑1 (MQ=255)

                         gAAAGCGAACGGCGGTGTAGCTGAAATGGATAAAATCAATCAGCAAAAAGCAGAACTGCTATATGGGGt                                <  1:3224266/69‑1 (MQ=255)

                         gAAAGCGAACGGCGGTGTAGCTGAAATGGATAAAATCAATCAGCAAAAAGCAGAACTGCTATATGGGGt                                <  1:2296259/69‑1 (MQ=255)

                         gAAAGCGAACGGCGGTGTAGCTGAAATGGATAAAATCAATCAGCAAAAAGCAGAACTGCTATATGGGGt                                <  1:1162490/69‑1 (MQ=255)

                           aaGCGAACGGCGGTGTGGCTGCAATGGATAAGATCAATCGGCAAAAAGCAGAACTGCt                                         <  1:1136373/58‑1 (MQ=255)

                           aaGCGAACGGCGGTGTGGCTGCAATGGATAAGATCAATCGGCAAAAAGCAGAACTGCt                                         <  1:1757322/58‑1 (MQ=255)

                           aaGCGAACGGCGGTGTGGCTGCAATGGATAAGATCAATCGGCAAAAAGCAGAACTGCt                                         <  1:2947172/58‑1 (MQ=255)

                           aaGCGAACGGCGGTGTGGCTGCAATGGATAAGATCAATCGGCAAAAAGCAGAACTGCt                                         <  1:1592440/58‑1 (MQ=255)

                           aaGCGAACGGCGGTGTGGCTGCAATGGATAAGATCAATCGGCAAAAAGCAGAACTGCt                                         <  1:3019674/58‑1 (MQ=255)

                           aaGCGAACGGCGGTGTGGCTGCAATGGATAAGATCAATCGGCAAAAAGCAGAACTGCt                                         <  1:1545554/58‑1 (MQ=255)

                           aaGCGAACGGCGGTGTGGCTGCAATGGATAAGATCAATCGGCAAAAAGCAGAACTGCt                                         <  1:1501116/58‑1 (MQ=255)

                           aaGCGAACGGCGGTGTGGCTGCAATGGATAAGATCAATCGGCAAAAAGCAGAACTGCt                                         <  1:319995/58‑1 (MQ=255)

                           aaGCGAACGGCGGTGTGGCTGCAATGGATAAGATCAATCGGCAAAAAGCAGAACTGCt                                         <  1:1350975/58‑1 (MQ=255)

                           aaGCGAACGGCGGTGTGGCTGCAATGGATAAGATCAATCGGCAAAAAGCAGAACTGCt                                         <  1:1169954/58‑1 (MQ=255)

                           aaGCGAACGGCGGTGTGGCTGCAATGGATAAGATCAATCGGCAAAAAGCAGAACTGCt                                         <  1:3130137/58‑1 (MQ=255)

                           aaGCGAACGGCGGTGTGGCTGCAATGGATAAGATCAATCGGCAAAAAGCAGAACTGCt                                         <  1:379234/58‑1 (MQ=255)

                           aaGCGAACGGCGGTGTGGCTGCAATGGATAAGATCAATCGGCAAAAAGCAGAACTGCt                                         <  1:453140/58‑1 (MQ=255)

                           aaGCGAACGGCGGTGTGGCTGCAATGGATAAGATCAATCGGCAAAAAGCAGAACTGCt                                         <  1:460258/58‑1 (MQ=255)

                           aaGCGAACGGCGGTGTGGCTGCAATGGATAAGATCAATCGGCAAAAAGCAGAACTGCt                                         <  1:547955/58‑1 (MQ=255)

                           aaGCGAACGGCGGTGTGGCTGCAATGGATAAGATCAATCGGCAAAAAGCAGAACTGCt                                         <  1:1104059/58‑1 (MQ=255)

                           aaGCGAACGGCGGTGTGGCTGCAATGGATAAGATCAATCGGCAAAAAGCAGAACTGCt                                         <  1:622849/58‑1 (MQ=255)

                           aaGCGAACGGCGGTGTGGCTGCAATGGATAAGATCAATCGGCAAAAAGCAGAACTGCt                                         <  1:873791/58‑1 (MQ=255)

                           aaGCGAACGGCGGTGTGGCTGCAATGGATAAGATCAATCGGCAAAAAGCAGAACTGCt                                         <  1:919356/58‑1 (MQ=255)

                           aaGCGAACGGCGGTGTGGCTGCAATGGATAAGATCAATCGGCAAAAAGCAGAACTGCt                                         <  1:2833632/58‑1 (MQ=255)

                           aaGCGAACGGCGGTGTGGCTGCAATGGATAAGATCAATCGGCAAAAAGCAGAACTGCt                                         <  1:2267373/58‑1 (MQ=255)

                           aaGCGAACGGCGGTGTGGCTGCAATGGATAAGATCAATCGGCAAAAAGCAGAACTGCt                                         <  1:2185599/58‑1 (MQ=255)

                           aaGCGAACGGCGGTGTGGCTGCAATGGATAAGATCAATCGGCAAAAAGCAGAACTGCt                                         <  1:2647785/58‑1 (MQ=255)

                           aaGCGAACGGCGGTGTGGCTGCAATGGATAAGATCAATCGGCAAAAAGCAGAACTGCt                                         <  1:2019909/58‑1 (MQ=255)

                           aaGCGAACGGCGGTGTGGCTGCAATGGATAAGATCAATCGGCAAAAAGCAGAACTGCt                                         <  1:2460681/58‑1 (MQ=255)

                           aaGCGAACGGCGGTGTGGCTGCAATGGATAAGATCAATCGGCAAAAAGCAGAACTGCt                                         <  1:1996429/58‑1 (MQ=255)

                                         gtAGCTGAAATGGATAAAATCAATCAGCAAAAAGCAGAACTGCTATATGGGGTGATTGATAACAGCGAt                <  1:2706987/69‑1 (MQ=255)

                                         gtAGCTGAAATGGATAAAATCAATCAGCAAAAAGCAGAACTGCTATATGGGGTGATTGATAACAGCGAt                >  1:1329035/1‑69 (MQ=255)

                                            gCTGCAATGGATAAGATCAATCGGCAAAAAGCAGAACTGCt                                         <  1:2983883/41‑1 (MQ=37)

                                            gCTGCAATGGATAAGATCAATCAGCAAAAAGCAGAACTGCtgt                                       >  1:780744/1‑41 (MQ=37)

                                            gCTGCAATGGATAAGATCAATCAGCAAAAAGCAGAACTGCtgt                                       >  1:2489990/1‑41 (MQ=37)

                                            gCTGCAATGGATAAGATCAATCAGCAAAAAGCAGAACTGCtgt                                       >  1:2514159/1‑41 (MQ=37)

                                            gCTGCAATGGATAAGATCAATCAGCAAAAAGCAGAACTGCtgt                                       >  1:2269432/1‑41 (MQ=37)

                                            gCTGCAATGGATAAGATCAATCAGCAAAAAGCAGAACTGCtgt                                       >  1:429285/1‑41 (MQ=37)

                                            gCTGCAATGGATAAGATCAATCAGCAAAAAGCAGAACTGCtgt                                       >  1:3422945/1‑41 (MQ=37)

                                            gCTGCAATGGATAAGATCAATCAGCAAAAAGCAGAACTGCtgt                                       >  1:1833439/1‑41 (MQ=37)

                                            gCTGCAATGGATAAGATCAATCAGCAAAAAGCAGAACTGCtgt                                       >  1:2985294/1‑41 (MQ=37)

                                            gCTGCAATGGATAAGATCAATCAGCAAAAAGCAGAACTGCtgt                                       >  1:2644190/1‑41 (MQ=37)

                                            gCTGCAATGGATAAGATCAATCAGCAAAAAGCAGAACTGCtgt                                       >  1:3130839/1‑41 (MQ=37)

                                            gCTGCAATGGATAAGATCAATAAGCAAAAAGCAGAACTGCtgt                                       >  1:1406881/1‑41 (MQ=37)

                                                   tGGATAAGATCAATCAGCAAAAAGCAGAACTGCTGTATGGGGTGATTGATAACAGCGATTTCTACCGc       >  1:816640/1‑68 (MQ=255)

                                                   tGGATAAGATCAATCAGCAAAAAGCAGAACTGCTGTATGGGGTGATTGATAACAGCGATTTCTACCGCa      >  1:566582/1‑69 (MQ=255)

                                                   tGGATAAGATCAATCAGCAAAAAGCAGAACTGCTGTATGGGGTGATTGATAACAGCGATTTCTACCGCa      >  1:2446284/1‑69 (MQ=255)

                                                   tGGATAAGATCAATCAGCAAAAAGCAGAACTGCTGTATGGGGTGATTGATAACAGCGATTTCTACCGCa      >  1:907189/1‑69 (MQ=255)

                                                   tGGATAAGATCAATCAGCAAAAAGCAGAACTGCTGTATGGGGTGATTGATAACAGCGATTTCTACCGCa      >  1:1080140/1‑69 (MQ=255)

                                                   tGGATAAGATCAATCAGCAAAAAGCAGAACTGCTGTATGGGGTGATTGATAACAGCGATTTCTACCGCa      >  1:635627/1‑69 (MQ=255)

                                                   tGGATAAGATCAATCAGCAAAAAGCAGAACTGCTGTATGGGGTGATTGATAACAGCGATTTCTACCGCa      >  1:555938/1‑69 (MQ=255)

                                                   tGGATAAGATCAATCAGCAAAAAGCAGAACTGCTGTATGGGGTGATTGATAACAGCGATTTCTACCGCa      >  1:2563299/1‑69 (MQ=255)

                                                   tGGATAAGATCAATCAGCAAAAAGCAGAACTGCTGTATGGGGTGATTGATAACAGCGATTTCTACCGCa      >  1:1171988/1‑69 (MQ=255)

                                                   tGGATAAGATCAATCAGCAAAAAGCAGAACTGCTGTATGGGGTGATTGATAACAGCGATTTCTACCGCa      >  1:1166947/1‑69 (MQ=255)

                                                       tAAAATCAATCAGCAAAAAGCAGAACTGCTATATGGGGTGATTGATAACAGCGATTTCTACCGCAATGa  <  1:2501745/69‑1 (MQ=255)

                                                       tAAAATCAATCAGCAAAAAGCAGAACTGCTATATGGGGTGATTGATAACAGCGATTTCTACCGCAATGa  <  1:2404681/69‑1 (MQ=255)

                                                       tAAAATCAATCAGCAAAAAGCAGAACTGCTATATGGGGTGATTGATAACAGCGATTTCTACCGCAATGa  <  1:1009190/69‑1 (MQ=255)

                                                       tAAAATCAATCAGCAAAAAGCAGAACTGCTATATGGGGTGATTGATAACAGCGATTTCTACCGCAATGa  <  1:2000090/69‑1 (MQ=255)

                                                       tAAAATCAATCAGCAAAAAGCAGAACTGCTATATGGGGTGATTGATAACAGCGATTTCTACCGCAATGa  <  1:256291/69‑1 (MQ=255)

TATCTGGTCTGGTCTTTAAATGGCTGAAAGCGAACGGCGGTGTAGCTGAAATGGATAAAATCAATCAGCAAAAAGCAGAACTGCTATATGGGGTGATTGATAACAGCGATTTCTACCGCAATGA  >  minE/615094‑615217

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A