Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
619,840 |
T→C |
56.7% |
L383L (TTG→CTG) |
rpsA → |
30S ribosomal subunit protein S1 |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 619,840 | 0 | T | C | 56.7%
| 16.2
/ 42.9
| 60 | L383L (TTG→CTG) | rpsA | 30S ribosomal subunit protein S1 |
| Reads supporting (aligned to +/- strand): ref base T (4/22); new base C (27/7); total (31/29) |
| Fisher's exact test for biased strand distribution p-value = 1.16e-06 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01 |
CACAACAAGGGCGACCGTGTTGAAGGTAAAATCAAGTCTATCACTGACTTCGGTATCTTCATCGGCTTGGACGGCGGCATCGACGGCCTGGTTCACCTGTCTGACATCTCCTGGAACGTTGCAG > minE/619774‑619897
|
cacaTCAAGGGCGACCGTGTTGAAGGTAAAATCAAGTCTATCACTGACTTCGGTATCTTCATCGGCTTg < 1:1177320/69‑1 (MQ=255)
cacaACAAGGGCGACCGTGTTGAAGGTAAAATCAAGTCTATCACTGACTTCGGTATCTTCATCGGCTTg < 1:1070879/69‑1 (MQ=255)
cacaACAAGGGCGACCGTGTTGAAGGTAAAATCAAGTCTATCACTGACTTCGGTATCTTCATCGGCTTg < 1:2650201/69‑1 (MQ=255)
cacaACAAGGGCGACCGTGTTGAAGGTAAAATCAAGTCTATCACTGACTTCGGTATCTTCATCGGCTTg < 1:2950149/69‑1 (MQ=255)
cacaACAAGGGCGACCGTGTTGAAGGTAAAATCAAGTCTATCACTGACTTCGGTATCTTCATCGGCTTg < 1:2477733/69‑1 (MQ=255)
cacaACAAGGGCGACCGTGTTGAAGGTAAAATCAAGTCTATCACTGACTTCGGTATCTTCATCGGCTTg < 1:3417303/69‑1 (MQ=255)
cacaACAAGGGCGACCGTGTTGAAGGTAAAATCAAGTCTATCACTGACTTCGGTATCTTCATCGGCTTg < 1:345100/69‑1 (MQ=255)
cacaACAAGGGCGACCGTGTTGAAGGTAAAATCAAGTCTATCACTGACTTCGGTATCTTCATCGGCTTg < 1:401389/69‑1 (MQ=255)
cacaACAAGGGCGACCGTGTTGAAGGTAAAATCAAGTCTATCACTGACTTCGGTATCTTCATCGGCTTg < 1:1744861/69‑1 (MQ=255)
cacaACAAGGGCGACCGTGTTGAAGGTAAAATCAAGTCTATCACTGACTTCGGTATCTTCATCGGCTTg < 1:1659960/69‑1 (MQ=255)
cacaACAAGGGCGACCGTGTTGAAGGTAAAATCAAGTCTATCACTGACTTCGGTATCTTCATCGGCTTg < 1:1616238/69‑1 (MQ=255)
cacaACAAGGGCGACCGTGTTGAAGGTAAAATCAAGTCTATCACTGACTTCGGTATCTTCATCGGCTTg < 1:159545/69‑1 (MQ=255)
cacaACAAGGGCGACCGTGTTGAAGGTAAAATCAAGTCTATCACTGACTTCGGTATCTTCATCGGCTTg < 1:1297371/69‑1 (MQ=255)
acaaGGGCGACCGTGTTGAAGGTAAAATCAAGTCTATCACTGACTTCGGTATCTTCATCGGCTTGGAcg > 1:1417243/1‑69 (MQ=255)
acaaGGGCGACCGTGTTGAAGGTAAAATCAAGTCTATCACTGACTTCGGTATCTTCATCGGCTTGGAcg > 1:270531/1‑69 (MQ=255)
acaaGGGCGACCGTGTTGAAGGTAAAATCAAGTCTATCACTGACTTCGGTATCTTCATCGGCTTGGAcg > 1:1012005/1‑69 (MQ=255)
tAAAATCAAGTCTATCACTGACTTCGGTATCTTCATCGGCTTGGACGGCGGCATCGACGGCCTGGTTCa < 1:2163479/69‑1 (MQ=255)
tAAAATCAAGTCTATCACTGACTTCGGTATCTTCATCGGCTTGGACGGCGGCATCGACGGCCTGGTTCa < 1:2381204/69‑1 (MQ=255)
tAAAATCAAGTCTATCACTGACTTCGGTATCTTCATCGGCTTGGACGGCGGCATCGACGGCCTGGTTCa < 1:2299335/69‑1 (MQ=255)
tAAAATCAAGTCTATCACTGACTTCGGTATCTTCATCGGCTTGGACGGCGGCATCGACGGCCTGGTTCa < 1:1537235/69‑1 (MQ=255)
tAAAATCAAGTCTATCACTGACTTCGGTATCTTCATCGGCTTGGACGGCGGCATCGACGGCCTGGTTCa < 1:3389611/69‑1 (MQ=255)
tAAAATCAAGTCTATCACTGACTTCGGTATCTTCATCGGCTTGGACGGCGGCATCGACGGCCTGGTTCa < 1:684389/69‑1 (MQ=255)
tAAAATCAAGTCTATCACTGACTTCGGTATCTTCATCGGCTTGGACGGCGGCATCGACGGCCTGGTTCa < 1:1294811/69‑1 (MQ=255)
tAAAATCAAGTCTATCACTGACTTCGGTATCTTCATCGGCTTGGACGGCGGCATCGACGGCCTGGTTCa < 1:1376759/69‑1 (MQ=255)
aaTCAAGTCTATCACTGACTTCGGTATCTTCATCGGCCTGGAcggcgg > 1:1200298/1‑48 (MQ=255)
aaTCAAGTCTATCACTGACTTCGGTATCTTCATCGGCCTGGACGGCGGCATCGACGGCCTTGTTc > 1:2606207/1‑65 (MQ=255)
aaTCAAGTCTATCACTGACTTCGGTATCTTCATCGGCCTGGACGGCGGCATCGACGGCCTGGtt > 1:41443/1‑64 (MQ=255)
aaTCAAGTCTATCACTGACTTCGGTATCTTCATCGGCCTGGACGGCGGCATCGACGGCCTGGTTc > 1:3092776/1‑65 (MQ=255)
aaTCAAGTCTATCACTGACTTCGGTATCTTCATCGGCCTGGACGGCGGCATCGACGGCCTGGTTc > 1:3033876/1‑65 (MQ=255)
aaTCAAGTCTATCACTGACTTCGGTATCTTCATCGGCCTGGACGGCGGCATCGACGGCCTGGTTc > 1:555009/1‑65 (MQ=255)
aaTCAAGTCTATCACTGACTTCGGTATCTTCATCGGCCTGGACGGCGGCATCGACGGCCTGGTTc > 1:624546/1‑65 (MQ=255)
aaTCAAGTCTATCACTGACTTCGGTATCTTCATCGGCCTGGACGGCGGCATCGACGGCCTGGTTc > 1:86794/1‑65 (MQ=255)
aaTCAAGTCTATCACTGACTTCGGTATCTTCATCGGCCTGGACGGCGGCATCGACGGCCTGGTTc > 1:2797181/1‑65 (MQ=255)
aaTCAAGTCTATCACTGACTTCGGTATCTTCATCGGCCTGGACGGCGGCATCGACGGCCTGGTTc > 1:271563/1‑65 (MQ=255)
aaTCAAGTCTATCACTGACTTCGGTATCTTCATCGGCCTGGACGGCGGCATCGACGGCCTGGTTc > 1:652556/1‑65 (MQ=255)
aaTCAAGTCTATCACTGACTTCGGTATCTTCATCGGCCTGGACGGCGGCATCGACGGCCTGGTTc > 1:2699819/1‑65 (MQ=255)
aaTCAAGTCTATCACTGACTTCGGTATCTTCATCGGCCTGGACGGCGGCATCGACGGCCTGGTTc > 1:950731/1‑65 (MQ=255)
aaTCAAGTCTATCACTGACTTCGGTATCTTCATCGGCCTGGACGGCGGCATCGACGGCCTGGTTc > 1:2563367/1‑65 (MQ=255)
aaTCAAGTCTATCACTGACTTCGGTATCTTCATCGGCCTGGACGGCGGCATCGACGGCCTGGTTc > 1:1135363/1‑65 (MQ=255)
aaTCAAGTCTATCACTGACTTCGGTATCTTCATCGGCCTGGACGGCGGCATCGACGGCCTGGTTc > 1:1275725/1‑65 (MQ=255)
aaTCAAGTCTATCACTGACTTCGGTATCTTCATCGGCCTGGACGGCGGCATCGACGGCCTGGTTc > 1:1628580/1‑65 (MQ=255)
aaTCAAGTCTATCACTGACTTCGGTATCTTCATCGGCCTGGACGGCGGCATCGACGGCCTGGTTc > 1:1705632/1‑65 (MQ=255)
aaTCAAGTCTATCACTGACTTCGGTATCTTCATCGGCCTGGACGGCGGCATCGACGGCCTGGTTc > 1:183880/1‑65 (MQ=255)
aaTCAAGTCTATCACTGACTTCGGTATCTTCATCGGCCTGGACGGCGGCATCGACGGCCTGGTTc > 1:1893395/1‑65 (MQ=255)
aaTCAAGTCTATCACTGACTTCGGTATCTTCATCGGCCTGGACGGCGGCATCGACGGCCTGGTTc > 1:1929684/1‑65 (MQ=255)
aaTCAAGTCTATCACTGACTTCGGTATCTTCATCGGCCTGGACGGCGGCATCGACGGCCTGGTTc > 1:1941866/1‑65 (MQ=255)
aaTCAAGTCTATCACTGACTTCGGTATCTTCATCGGCCTGGACGGCGGCATCGACGGCCTGGTTc > 1:2072717/1‑65 (MQ=255)
aaTCAAGTCTATCACTGACTTCGGTATCTTCATCGGCCTGGACGGCGGCATCGACGGCCTGGTTc > 1:2488167/1‑65 (MQ=255)
aaTCAAGTCTATCACTGACTTCGGTATCTTCATCGGCCTGGACGGCGGCATCGACGGCCTGGTTc > 1:2522291/1‑65 (MQ=255)
aaTCAAGTCTATCACTGACTTCGGTATCTTCATCGGCCTGGACGGCGGCATCGACGGCCTGGTTc > 1:2610896/1‑65 (MQ=255)
aaTCAAGTCTATCACTGACTTCGGTATCTTCATCGGCCT‑GACGGCGGCATCGACGGCCTGGTTc > 1:2522317/1‑64 (MQ=255)
cAAGTCTATCACTGACTTCGGTATCTTCATCGGCTTGGACGGCGGCATCGACGGCCTGGTTCACCTGt > 1:2958611/1‑68 (MQ=255)
tCTATCACTGACTTCGGTATCTTCATCGGCTTGGACGGCGGCATCGACGGCCTGGTTCACCTGTCTGAc < 1:894805/69‑1 (MQ=255)
tCTTCATCGGCCTGGACGGCGGCATCGACGGCCTGGTTCACCTGTCTGACATCTCCTGGAACGTTGCAg < 1:2177191/69‑1 (MQ=255)
tCTTCATCGGCCTGGACGGCGGCATCGACGGCCTGGTTCACCTGTCTGACATCTCCTGGAACGTTGCAg < 1:1958406/69‑1 (MQ=255)
tCTTCATCGGCCTGGACGGCGGCATCGACGGCCTGGTTCACCTGTCTGACATCTCCTGGAACGTTGCAg < 1:2247552/69‑1 (MQ=255)
tCTTCATCGGCCTGGACGGCGGCATCGACGGCCTGGTTCACCTGTCTGACATCTCCTGGAACGTTGCAg < 1:2854269/69‑1 (MQ=255)
tCTTCATCGGCCTGGACGGCGGCATCGACGGCCTGGTTCACCTGTCTGACATCTCCTGGAACGTTGCAg < 1:271132/69‑1 (MQ=255)
tCTTCATCGGCCTGGACGGCGGCATCGACGGCCTGGTTCACCTGTCTGACATCTCCTGGAACGTTGCAg < 1:1138748/69‑1 (MQ=255)
cTTCATCGGCCTGGACGGCGGCATCGACGGCCTGGTTCACCTGTCTGACATCTCCTGGAACGTTGCAg < 1:849121/68‑1 (MQ=255)
|
CACAACAAGGGCGACCGTGTTGAAGGTAAAATCAAGTCTATCACTGACTTCGGTATCTTCATCGGCTTGGACGGCGGCATCGACGGCCTGGTTCACCTGTCTGACATCTCCTGGAACGTTGCAG > minE/619774‑619897
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
