Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
634,576 |
C→T |
46.5% |
L518L (CTG→TTG) |
mukB → |
fused chromosome partitioning proteins |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 634,576 | 0 | C | T | 46.5%
| 5.8
/ 46.5
| 58 | L518L (CTG→TTG) | mukB | fused chromosome partitioning proteins |
| Reads supporting (aligned to +/- strand): ref base C (22/9); new base T (4/23); total (26/32) |
| Fisher's exact test for biased strand distribution p-value = 1.94e-05 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01 |
TCGCGAACTATTGCGCGAAGGGGTCGATCAGCGTCACCTGGCAGAGCAGGTTCAGCCGCTGCGGATGCGATTAAGCGAACTGGAACAGCGTCTACGCGAGCAGCAAGAAGCTGAGCGTCTGCTGGCA > minE/634518‑634644
|
tcgcGAACTATTGCGCGAAGGGGTCGATCAGCGTCACCTGGCAGAGCAGGTTCAGCCGCTGCGGATGc < 1:1997735/68‑1 (MQ=255)
cgcgAACTATTGCGCGAAGGGGTCGATCAGCGTCACCTGGCAGAGCAGGTTCAGCCGCTGCGGATGCg > 1:2471483/1‑68 (MQ=255)
cgcgAACTATTGCGCGAAGGGGTCGATCAGCGTCACCTGGCAGAGCAGGTTCAGCCGCTGCGGATGCg > 1:2855887/1‑68 (MQ=255)
ttGCGCGAAGGGGTCGATCAGCGTCACCTGGCAGAGCAGGTTCAGCCGCTGCGGATGCGATTAAGCGaa > 1:2501500/1‑69 (MQ=255)
ttGCGCGAAGGGGTCGATCAGCGTCACCTGGCAGAGCAGGTTCAGCCGCTGCGGATGCGATTAAGCGaa > 1:2299641/1‑69 (MQ=255)
ttGCGCGAAGGGGTCGATCAGCGTCACCTGGCAGAGCAGGTTCAGCCGCTGCGGATGCGATTAAGCGaa > 1:1119803/1‑69 (MQ=255)
ttGCGCGAAGGGGTCGATCAGCGTCACCTGGCAGAGCAGGTTCAGCCGCTGCGGATGCGATTAAGCGaa > 1:3146750/1‑69 (MQ=255)
ttGCGCGAAGGGGTCGATCAGCGTCACCTGGCAGAGCAGGTTCAGCCGCTGCGGATGCGATTAAGCGaa > 1:1747045/1‑69 (MQ=255)
ttGCGCGAAGGGGTCGATCAGCGTCACCTGGCAGAGCAGGTTCAGCCGCTGCGGATGCGATTAAGCGaa > 1:1479720/1‑69 (MQ=255)
ttGCGCGAAGGGGTCGATCAGCGTCACCTGGCAGAGCAGGTTCAGCCGCTGCGGATGCGATTAAGCGaa > 1:45615/1‑69 (MQ=255)
ttGCGCGAAGGGGTCGATCAGCGTCACCTGGCAGAGCAGGTTCAGCCGCTGCGGATGCGATTAAGCGa > 1:3230675/1‑68 (MQ=255)
ttGCGAGAAGGGGTCGATCAGCGTCACCTGGCAGAGCAGGTTCAGCCGCTGCGGATGCGATTAAGCGaa > 1:3039307/1‑69 (MQ=255)
tGCGCGAAGGGGTCGATCAGCGTCACCTGGCAGAGCAGGTTCAGCCGCTGCGGATGCGATTAAGCg > 1:789670/1‑66 (MQ=255)
cgAAGGGGTCGATCAGCGTCACCTGGCATAGCAGGTTCAGCCGCTGCGGATGCGATTAAGCGAACTgg < 1:1279710/68‑1 (MQ=255)
cgAAGGGGTCGATCAGCGTCACCTGGCAGAGCAGGTTCAGCCGCTGCGGATGCGATTAAGCGAACTgg < 1:1894456/68‑1 (MQ=255)
cgAAGGGGTCGATCAGCGTCACCTGGCAGAGCAGGTTCAGCCGCTGCGGATGCGATTAAGCGAACTgg < 1:111115/68‑1 (MQ=255)
cgAAGGGGTCGATCAGCGTCACCTGGCAGAGCAGGTTCAGCCGCTGCGGATGCGATTAAGCGAACTgg < 1:2266450/68‑1 (MQ=255)
cgAAGGGGTCGATCAGCGTCACCTGGCAGAGCAGGTTCAGCCGCTGCGGATGCCATTAAGCGAACTgg < 1:3376759/68‑1 (MQ=255)
gggTCGATCAGCGTCACCTGGCAGAGCAGGTTCAGCCGCTGCGGATGCGATTAAGCGAACTGGAACAGc > 1:2816144/1‑69 (MQ=255)
gTCGATCAGCGTCACCTGGCAGAGCAGGTTCAGCCGTTGCGGATGCGCTTAAGCGAACTGGAACAGCGt < 1:2976428/69‑1 (MQ=255)
gTCGATCAGCGTCACCTGGCAGAGCAGGTTCAGCCGTTGCGGATGCGCTTAAGCGAACTGGAACAGCGt < 1:981558/69‑1 (MQ=255)
gTCGATCAGCGTCACCTGGCAGAGCAGGTTCAGCCGTTGCGGATGCGCTTAAGCGAACTGGAACAGCGt < 1:2735088/69‑1 (MQ=255)
gTCGATCAGCGTCACCTGGCAGAGCAGGTTCAGCCGTTGCGGATGCGCTTAAGCGAACTGGAACAGCGt < 1:633206/69‑1 (MQ=255)
gTCGATCAGCGTCACCTGGCAGAGCAGGTTCAGCCGTTGCGGATGCGCTTAAGCGAACTGGAACAGCGt < 1:2918566/69‑1 (MQ=255)
gTCGATCAGCGTCACCTGGCAGAGCAGGTTCAGCCGTTGCGGATGCGCTTAAGCGAACTGGAACAGCGt < 1:621023/69‑1 (MQ=255)
gTCGATCAGCGTCACCTGGCAGAGCAGGTTCAGCCGTTGCGGATGCGCTTAAGCGAACTGGAACAGCGt < 1:623343/69‑1 (MQ=255)
gTCGATCAGCGTCACCTGGCAGAGCAGGTTCAGCCGTTGCGGATGCGCTTAAGCGAACTGGAACAGCGt < 1:1015414/69‑1 (MQ=255)
gTCGATCAGCGTCACCTGGCAGAGCAGGTTCAGCCGTTGCGGATGCGCTTAAGCGAACTGGAACAGCGt < 1:3403309/69‑1 (MQ=255)
gTCGATCAGCGTCACCTGGCAGAGCAGGTTCAGCCGTTGCGGATGCGCTTAAGCGAACTGGAACAGCGt < 1:1867107/69‑1 (MQ=255)
gTCGATCAGCGTCACCTGGCAGAGCAGGTTCAGCCGTTGCGGATGCGCTTAAGCGAACTGGAACAGCGt < 1:1003077/69‑1 (MQ=255)
gTCGATCAGCGTCACCTGGCAGAGCAGGTTCAGCCGTTGCGGATGCGCTTAAGCGAACTGGAACAGCGt < 1:1362912/69‑1 (MQ=255)
gTCGATCAGCGTCACCTGGCAGAGCAGGTTCAGCCGTTGCGGATGCGCTTAAGCGAACTGGAACAGCGt < 1:1716397/69‑1 (MQ=255)
gTCGATCAGCGTCACCTGGCAGAGCAGGTTCAGCCGTTGCGGATGCGCTTAAGCGAACTGGAACAGCGt < 1:1794336/69‑1 (MQ=255)
gTCGATCAGCGTCACCTGGCAGAGCAGGTTCAGCCGTTGCGGATGCGCTTAAGCGAACTGGAACAGCGt < 1:1982910/69‑1 (MQ=255)
gTCGATCAGCGTCACCTGGCAGAGCAGGTTCAGCCGTTGCGGATGCGCTTAAGCGAACTGGAACAGCGt < 1:2105047/69‑1 (MQ=255)
gTCGATCAGCGTCACCTGGCAGAGCAGGTTCAGCCGTTGCGGATGCGCTTAAGCGAACTGGAACAGCGt < 1:2230329/69‑1 (MQ=255)
gTCGATCAGCGTCACCTGGCAGAGCAGGTTCAGCCGTTGCGGATGCGCTTAAGCGAACTGGAACAGCGt < 1:235974/69‑1 (MQ=255)
tCGATCAGCGTCACCTGGCAGAGCAGGTTCAGCCGTTGCGGATGCGCTTAAGCGAACTGGAACAGCGt < 1:461175/68‑1 (MQ=255)
tCGATCAGCGTCACCTGGCAGAGCAGGTTCAGCCGTTGCGGATGCGCTTAAGCGAACTGGAACAGCGt < 1:2434888/68‑1 (MQ=255)
cGTCACCTGGCAGAGCAGGTTCAGCCGTTGCGGATGCGCTTAAGCGAACTGGAACAGCGt < 1:264855/60‑1 (MQ=255)
tCACCTGGCAGAGCAGGTTCAGCCGCTGCGGATGCGATTAAGCGAACTGGAACAGCGTCTACGCGagc > 1:1034511/1‑68 (MQ=255)
tCACCTGGCAGAGCAGGTTCAGCCGCTGCGGATGCGATTAAGCGAACTGGAACAGCGTCTACGCGagc > 1:742866/1‑68 (MQ=255)
tCACCTGGCAGAGCAGGTTCAGCCGCTGCGGATGCGATTAAGCGAACTGGAACAGCGTCTACGCGagc > 1:1900350/1‑68 (MQ=255)
aCCTGGCAGAGCAGGTTCAGCCGTTGCGGATGCGCTTAAGCGTACTGGAACAGCGTCTGCGTGagca > 1:2218468/1‑67 (MQ=255)
aCCTGGCAGAGCAGGTTCAGCCGTTGCGGATGCGCTTAAGCGAACTGGAACAGCGTCTGCGTGagca > 1:961619/1‑67 (MQ=255)
aCCTGGCAGAGCAGGTTCAGCCGTTGCGGATGCGCTTAAGCGAACTGGAACAGCGTCTGCGTGagca > 1:3400591/1‑67 (MQ=255)
aCCTGGCAGAGCAGGTTCAGCCGTTGCGGATGCGCTTAAGCGAACTGGAACAGCGTCTGCGTGagc > 1:881425/1‑66 (MQ=255)
ccTGGCAGAGCAGGTTCAGCCGTTGCGGATGCGCTTAAGCGAACTGGAACAGCGt < 1:2502463/55‑1 (MQ=255)
cAGGTTCAGCCGCTGCGGATGCGATTAAGCGAACTGGAACAGCGTCTACGCGAGCAGCAAGAAGGTGAg > 1:2987077/1‑69 (MQ=255)
cAGGTTCAGCCGCTGAGGATGCGATTAAGCGAACTGGAACAGCGTCTACGCGAGCAGCAAGAAGCTGAg > 1:3397770/1‑69 (MQ=255)
ggTTCAGCCGCTGCGGATGCGATTAAGCGAACTGGAACAGCGTCTACGCGAGCAGCAAGAAGCTGAGCg > 1:2933701/1‑69 (MQ=255)
ttCAGCCGCTGCGGATGCGATTAAGCGAACTGGAACAGCGTCTACGCGAGCAGCAAGAAGCTGAGCGTc < 1:2991857/69‑1 (MQ=255)
ttCAGCCGCTGCGGATGCGATTAAGCGAACTGGAACAGCGTCTACGCGAGCAGCAAGAAGCTGAGCGTc < 1:2519368/69‑1 (MQ=255)
ttCAGCCGCTGCGGATGCGATTAAGCGAACTGGAACAGCGTCTACGCGAGCAGCAAGAAGCTGAGCGTc < 1:1059388/69‑1 (MQ=255)
tCAGCCGCTGCGGATGCGATTAAGCGAACTGGAACAGCGTCTACGCGAGCAGCAAGAAGCTGAGCGTct > 1:1750044/1‑69 (MQ=255)
tCAGCCGCTGCGGATGCGATTAAGCGAACTGGAACAGCGTCTACGCGAGCAGCAAGAAGCTGAGCGTct > 1:3418083/1‑69 (MQ=255)
tCAGCCGCTGCGGATGCGATTAAGCGAACTGGAACAGCGTCTACGCGAGCAGCAAGAAGCTGAGCGTct > 1:914845/1‑69 (MQ=255)
cAGCCGTTGCGGATGCGCTTAAGCGAACTGGAACAGCGt < 1:2746563/39‑1 (MQ=37)
cTGCGGATGCGATTAAGCGAACTGGAACAGCGTCTACGCGAGCAGCAAGAAGCTGAGCGTCTGCTGGCa < 1:2357181/69‑1 (MQ=255)
cTGCGGATGCGATTAAGCGAACTGGAACAGCGTCTACGCGAGCAGCAAGAAGCTGAGCGTCTGCTGGCa < 1:875047/69‑1 (MQ=255)
cTGCGGATGCGATTAAGCGAACTGGAACAGCGTCTACGCGAGCAGCAAGAAGCTGAGCGTCTGCTGGCa < 1:2982265/69‑1 (MQ=255)
cTGCGGATGCGATTAAGCGAACTGGAACAGCGTCTACGCGAGCAGCAAGAAGCTGAGCGTCTGCTGGCa < 1:753567/69‑1 (MQ=255)
cTGCGGATGCGATTAAGCGAACTGGAACAGCGTCTACGCGAGCAGCAAGAAGCTGAGCGTCTGCTGGCa < 1:1386724/69‑1 (MQ=255)
cTGCGGATGCGATTAAGCGAACTGGAACAGCGTCTACGCGAGCAGCAAGAAGCTGAGCGTCTGCTGGCa < 1:2592138/69‑1 (MQ=255)
cTGCGGATGCGATTAAGCGAACTGGAACAGCGTCTACGCGAGCAGCAAGAAGCTGAGCGTCTGCTGGCa < 1:3224818/69‑1 (MQ=255)
|
TCGCGAACTATTGCGCGAAGGGGTCGATCAGCGTCACCTGGCAGAGCAGGTTCAGCCGCTGCGGATGCGATTAAGCGAACTGGAACAGCGTCTACGCGAGCAGCAAGAAGCTGAGCGTCTGCTGGCA > minE/634518‑634644
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
