Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
642,198 |
T→G |
84.4% |
I71L (ATC→CTC) |
aspC ← |
aspartate aminotransferase, PLP‑dependent |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 642,198 | 0 | T | G | 84.4%
| 180.2
/ 18.0
| 83 | I71L (ATC→CTC) | aspC | aspartate aminotransferase, PLP‑dependent |
| Reads supporting (aligned to +/- strand): ref base T (5/8); new base G (56/14); total (61/22) |
| Fisher's exact test for biased strand distribution p-value = 4.13e-03 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
ATTGATCAGGGCGCTACCTTTACCAAACAGCAGTTCCTGAGTGCAGCGACCAAATTCAGGGATGCCGTCAATGCCGAGGTAATTTTTGGTGGTTTCATTTTCGAGCAGATACTGTTCAGCCTTT > minE/642136‑642259
|
aTTGATCAGGGCGCTACCTTTACCAAACAGCAGTTCCTGAGTGCAGCGACCAAATTCAGGGATGCCGTc < 1:1131192/69‑1 (MQ=255)
aTTGATCAGGGCGCTACCTTTACCAAACAGCAGTTCCTGAGTGCAGCGACCAAATTCAGGGATGCCGTc < 1:2079596/69‑1 (MQ=255)
aTTGATCAGGGCGCTACCTTTACCAAACAGCAGTTCCTGAGTGCAGCGACCAAATTCAGGGATGCCGTc < 1:687594/69‑1 (MQ=255)
aTTGATCAGGGCGCTACCTTTACCAAACAGCAGTTCCTGAGTGCAGCGACCAAATTCAGGGATGCCGTc < 1:1648340/69‑1 (MQ=255)
aTTGATCAGGGCGCTACCTTTACCAAACAGCAGTTCCTGAGTGCAGCGACCAAATTCAGGGATGCCGTc < 1:1538608/69‑1 (MQ=255)
aTTGATCAGGGCGCTACCTTTACCAAACAGCAGTTCCTGAGTGCAGCGACCAAATTCAGGGATGCCGTc < 1:212379/69‑1 (MQ=255)
aTTGATCAGGGCGCTACCTTTACCAAACAGCAGTTCCTGAGTGCAGCGACCAAATTCAGGGATGCCGTc < 1:2152400/69‑1 (MQ=255)
ttGATCAGGGCGCTACCTTTACCAAACAGCAGTTCCTGAGTGCAGCGACCAAATTCAGGGAGGcc > 1:596964/1‑65 (MQ=255)
ttGATCAGGGCGCTACCTTTACCAAACAGCAGTTCCTGAGTGCAGCGACCAAATTCAGGGAGGCCg > 1:1667668/1‑66 (MQ=255)
ttGATCAGGGCGCTACCTTTACCAAACAGCAGTTCCTGAGTGCAGCGACCAAATTCAGGGAGGCCg > 1:2065710/1‑66 (MQ=255)
ttGATCAGGGCGCTACCTTTACCAAACAGCAGTTCCTGAGTGCAGCGACCAAATTCAGGGAGGCCg > 1:1989020/1‑66 (MQ=255)
ttGATCAGGGCGCTACCTTTACCAAACAGCAGTTCCTGAGTGCAGCGACCAAATTCAGGGAGGCCg > 1:3043022/1‑66 (MQ=255)
ttGATCAGGGCGCTACCTTTACCAAACAGCAGTTCCTGAGTGCAGCGACCAAATTCAGGGAGGCCg > 1:241033/1‑66 (MQ=255)
ttGATCAGGGCGCTACCTTTACCAAACAGCAGTTCCTGAGTGCAGCGACCAAATTCAGGGAGGCCg > 1:2432897/1‑66 (MQ=255)
ttGATCAGGGCGCTACCTTTACCAAACAGCAGTTCCTGAGTGCAGCGACCAAATTCAGGGAGGCCg > 1:1849933/1‑66 (MQ=255)
ttGATCAGGGCGCTACCTTTACCAAACAGCAGTTCCTGAGTGCAGCGACCAAATTCAGGGAGGCCg > 1:3353708/1‑66 (MQ=255)
ttGATCAGGGCGCTACCTTTACCAAACAGCAGTTCCTGAGTGCAGCGACCAAATTCAGGGAGGCCg > 1:3420288/1‑66 (MQ=255)
ttGATCAGGGCGCTACCTTTACCAAACAGCAGTTCCTGAGTGCAGCGACCAAATTCAGGGAGGCCg > 1:534814/1‑66 (MQ=255)
ttGATCAGGGCGCTACCTTTACCAAACAGCAGTTCCTGAGTGCAGCGACCAAATTCAGGGAGGCCg > 1:1615415/1‑66 (MQ=255)
ttGATCAGGGCGCTACCTTTACCAAACAGCAGTTCCTGAGTGCAGCGACCAAATTCAGGGAGGCCg > 1:2467988/1‑66 (MQ=255)
ttGATCAGGGCGCTACCTTTACCAAACAGCAGTTCCTGAGTGCAGCGACCAAATTCAGGGAGGCCg > 1:1565861/1‑66 (MQ=255)
ttGATCAGGGCGCTACCTTTACCAAACAGCAGTTCCTGAGTGCAGCGACCAAATTCAGGGAGGCCg > 1:2270396/1‑66 (MQ=255)
ttGATCAGGGCGCTACCTTTACCAAACAGCAGTTCCTGAGTGCAGCGACCAAATTCAGGGAGGCCg > 1:614015/1‑66 (MQ=255)
ttGATCAGGGCGCTACCTTTACCAAACAGCAGTTCCTGAGTGCAGCGACCAAATTCAGGGAGGCCg > 1:1452937/1‑66 (MQ=255)
ttGATCAGGGCGCTACCTTTACCAAACAGCAGTTCCTGAGTGCAGCGACCAAATTCAGGGAGGCCg > 1:2535981/1‑66 (MQ=255)
cAGGGCGCTACCTTTACCAAACAGCAGTTCCTGAGTGCAGCGACCAAATTCAGGGATg > 1:489499/1‑58 (MQ=255)
cAGGGCGCTACCTTTACCAAACAGCAGTTCCTGAGTGCAGCGACCAAATTCAGGGATGCCGTCAAt > 1:2597295/1‑66 (MQ=255)
cAGGGCGCTACCTTTACCAAACAGCAGTTCCTGAGTGCAGCGACCAAATTCAGGGATGCCGTCAATg > 1:3130669/1‑67 (MQ=255)
ttACCAAACAGCAGTTCCTGAGTGCAGCGCCCAAATTCAGGGAGGCCGTCAATGCCGAGGTAATTTTtg > 1:2822549/1‑69 (MQ=255)
ttACCAAACAGCAGTTCCTGAGTGCAGCGACCAAATTCAGGGAGGCCGTCAATGCCGAGGTAATTTTtg > 1:2457184/1‑69 (MQ=255)
ttACCAAACAGCAGTTCCTGAGTGCAGCGACCAAATTCAGGGAGGCCGTCAATGCCGAGGTAATTTTtg > 1:3299535/1‑69 (MQ=255)
ttACCAAACAGCAGTTCCTGAGTGCAGCGACCAAATTCAGGGAGGCCGTCAATGCCGAGGTAATTTTtg > 1:3271732/1‑69 (MQ=255)
ttACCAAACAGCAGTTCCTGAGTGCAGCGACCAAATTCAGGGAGGCCGTCAATGCCGAGGTAATTTTtg > 1:3225696/1‑69 (MQ=255)
ttACCAAACAGCAGTTCCTGAGTGCAGCGACCAAATTCAGGGAGGCCGTCAATGCCGAGGTAATTTTtg > 1:402424/1‑69 (MQ=255)
ttACCAAACAGCAGTTCCTGAGTGCAGCGACCAAATTCAGGGAGGCCGTCAATGCCGAGGTAATTTTtg > 1:3043826/1‑69 (MQ=255)
ttACCAAACAGCAGTTCCTGAGTGCAGCGACCAAATTCAGGGAGGCCGTCAATGCCGAGGTAATTTTtg > 1:540782/1‑69 (MQ=255)
ttACCAAACAGCAGTTCCTGAGTGCAGCGACCAAATTCAGGGAGGCCGTCAATGCCGAGGTAATTTTtg > 1:1205680/1‑69 (MQ=255)
ttACCAAACAGCAGTTCCTGAGTGCAGCGACCAAATTCAGGGAGGCCGTCAATGCCGAGGTAATTTTtg > 1:1273538/1‑69 (MQ=255)
ttACCAAACAGCAGTTCCTGAGTGCAGCGACCAAATTCAGGGAGGCCGTCAATGCCGAGGTAATTTTtg > 1:1313204/1‑69 (MQ=255)
ttACCAAACAGCAGTTCCTGAGTGCAGCGACCAAATTCAGGGAGGCCGTCAATGCCGAGGTAATTTTtg > 1:1318626/1‑69 (MQ=255)
ttACCAAACAGCAGTTCCTGAGTGCAGCGACCAAATTCAGGGAGGCCGTCAATGCCGAGGTAATTTTtg > 1:1327459/1‑69 (MQ=255)
ttACCAAACAGCAGTTCCTGAGTGCAGCGACCAAATTCAGGGAGGCCGTCAATGCCGAGGTAATTTTtg > 1:1348801/1‑69 (MQ=255)
ttACCAAACAGCAGTTCCTGAGTGCAGCGACCAAATTCAGGGAGGCCGTCAATGCCGAGGTAATTTTtg > 1:760987/1‑69 (MQ=255)
ttACCAAACAGCAGTTCCTGAGTGCAGCGACCAAATTCAGGGAGGCCGTCAATGCCGAGGTAATTTTtg > 1:69605/1‑69 (MQ=255)
ttACCAAACAGCAGTTCCTGAGTGCAGCGACCAAATTCAGGGAGGCCGTCAATGCCGAGGTAATTTTtg > 1:1795399/1‑69 (MQ=255)
ttACCAAACAGCAGTTCCTGAGTGCAGCGACCAAATTCAGGGAGGCCGTCAATGCCGAGGTAATTTTtg > 1:1898798/1‑69 (MQ=255)
ttACCAAACAGCAGTTCCTGAGTGCAGCGACCAAATTCAGGGAGGCCGTCAATGCCGAGGTAATTTTtg > 1:1928500/1‑69 (MQ=255)
ttACCAAACAGCAGTTCCTGAGTGCAGCGACCAAATTCAGGGAGGCCGTCAATGCCGAGGTAATTTTtg > 1:643867/1‑69 (MQ=255)
aaCAGCAGTTCCTGAGTGCAGCGACCAAATTCAGGGAGGCCGTCAATg < 1:694718/48‑1 (MQ=255)
aaCAGCAGTTCCTGAGTGCAGCGACCAAATTCAGGGAGGCCGTCAATg < 1:691399/48‑1 (MQ=255)
aaCAGCAGTTCCTGAGTGCAGCGACCAAATTCAGGGAGGCCGTCAATg < 1:48484/48‑1 (MQ=255)
aaCAGCAGTTCCTGAGTGCAGCGACCAAATTCAGGGAGGCCGTCAATg < 1:87604/48‑1 (MQ=255)
aaCAGCAGTTCCTGAGTGCAGCGACCAAATTCAGGGAGGCCGTCAATg < 1:2934682/48‑1 (MQ=255)
aaCAGCAGTTCCTGAGTGCAGCGACCAAATTCAGGGAGGCCGTCAATg < 1:1157993/48‑1 (MQ=255)
aaCAGCAGTTCCTGAGTGCAGCGACCAAATTCAGGGAGGCCGTCAATg < 1:1536974/48‑1 (MQ=255)
aaCAGCAGTTCCTGAGTGCAGCGACCAAATTCAGGGAGGCCGTCAATg < 1:1751041/48‑1 (MQ=255)
aaCAGCAGTTCCTGAGTGCAGCGACCAAATTCAGGGAGGCCGTCAATg < 1:2104154/48‑1 (MQ=255)
aaCAGCAGTTCCTGAGTGCAGCGACCAAATTCAGGGAGGCCGTCAATg < 1:2364927/48‑1 (MQ=255)
aaCAGCAGTTCCTGAGTGCAGCGACCAAATTCAGGGAGGCCGTCAATg < 1:2369000/48‑1 (MQ=255)
aaCAGCAGTTCCTGAGTGCAGCGACCAAATTCAGGGAGGCCGTCAATg < 1:237635/48‑1 (MQ=255)
aaCAGCAGTTCCTGAGTGCAGCGACCAAATTCAGGGAGGCCGTCAATg < 1:2486533/48‑1 (MQ=255)
aaCAGCAGTTCCTGAGTGCAGCGACCAAATTCAGGGAGGCCGTCAATg < 1:2795700/48‑1 (MQ=255)
gAGTGCAGCGACCAAATTCAGGGATGCCGTCAATGCCGAGGTAATTTTTGGTGGTTTCATTTTCGAGCa < 1:866182/69‑1 (MQ=255)
cAGCGACCAAATTCAGGGATGCCGTCAATGCCGAGGTAATTTTTGGTGGTTTCATTTTCGAGCAGATAc > 1:83229/1‑69 (MQ=255)
cAGCGAACAAATTCAGGGATGCCGTCAATGCCGAGGTAATTTTTGGTGGTTTCATTTTCGAGCAGATAc > 1:6764/1‑69 (MQ=255)
tCAGGGAGGCCGTCAATGCCGAGGTAATTTTTGGTGGTTTCATTTTCGAGCAGATa > 1:526816/1‑56 (MQ=255)
tCAGGGAGGCCGTCAATGCCGAGGTAATTTTTGGTGGTTTCATTTTCGAGCAGATACTGTTCAGCCttt > 1:3391714/1‑69 (MQ=255)
tCAGGGAGGCCGTCAATGCCGAGGTAATTTTTGGTGGTTTCATTTTCGAGCAGATACTGTTCAGCCttt > 1:829414/1‑69 (MQ=255)
tCAGGGAGGCCGTCAATGCCGAGGTAATTTTTGGTGGTTTCATTTTCGAGCAGATACTGTTCAGCCttt > 1:1448689/1‑69 (MQ=255)
tCAGGGAGGCCGTCAATGCCGAGGTAATTTTTGGTGGTTTCATTTTCGAGCAGATACTGTTCAGCCttt > 1:318195/1‑69 (MQ=255)
tCAGGGAGGCCGTCAATGCCGAGGTAATTTTTGGTGGTTTCATTTTCGAGCAGATACTGTTCAGCCttt > 1:1602905/1‑69 (MQ=255)
tCAGGGAGGCCGTCAATGCCGAGGTAATTTTTGGTGGTTTCATTTTCGAGCAGATACTGTTCAGCCttt > 1:3220534/1‑69 (MQ=255)
tCAGGGAGGCCGTCAATGCCGAGGTAATTTTTGGTGGTTTCATTTTCGAGCAGATACTGTTCAGCCttt > 1:1973732/1‑69 (MQ=255)
tCAGGGAGGCCGTCAATGCCGAGGTAATTTTTGGTGGTTTCATTTTCGAGCAGATACTGTTCAGCCttt > 1:2084519/1‑69 (MQ=255)
tCAGGGAGGCCGTCAATGCCGAGGTAATTTTTGGTGGTTTCATTTTCGAGCAGATACTGTTCAGCCttt > 1:603053/1‑69 (MQ=255)
tCAGGGAGGCCGTCAATGCCGAGGTAATTTTTGGTGGTTTCATTTTCGAGCAGATACTGTTCAGCCttt > 1:2235705/1‑69 (MQ=255)
tCAGGGAGGCCGTCAATGCCGAGGTAATTTTTGGTGGTTTCATTTTCGAGCAGATACTGTTCAGCCttt > 1:2271693/1‑69 (MQ=255)
tCAGGGAGGCCGTCAATGCCGAGGTAATTTTTGGTGGTTTCATTTTCGAGCAGATACTGTTCAGCCttt > 1:2305381/1‑69 (MQ=255)
tCAGGGAGGCCGTCAATGCCGAGGTAATTTTTGGTGGTTTCATTTTCGAGCAGATACTGTTCAGCCttt > 1:500316/1‑69 (MQ=255)
tCAGGGAGGCCGTCAATGCCGAGGTAATTTTTGGTGGTTTCATTTTCGAGCAGATACTGTTCAGCCttt > 1:2372077/1‑69 (MQ=255)
tCAGGGAGGCCGTCAATGCCGAGGTAATTTTTGGTGGTTTCATTTTCGAGCAGATACTGTTCAGCCttt > 1:2451312/1‑69 (MQ=255)
tCAGGGAGGCCGTCAATGCCGAGGTAATTTTTGGTGGTTTCATTTTCGAGCAGATACTGTTCAGCCtt > 1:3044027/1‑68 (MQ=255)
tCAGGGAGGCCGTCAATGCCGAGGTAATTTTTGGTGGTTTCATTTTCGAGCAGATACTGTTCAGCCtt > 1:2796290/1‑68 (MQ=255)
|
ATTGATCAGGGCGCTACCTTTACCAAACAGCAGTTCCTGAGTGCAGCGACCAAATTCAGGGATGCCGTCAATGCCGAGGTAATTTTTGGTGGTTTCATTTTCGAGCAGATACTGTTCAGCCTTT > minE/642136‑642259
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
