Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
647,941 |
C→T |
37.0% |
R207R (CGC→CGT) |
pepN → |
aminopeptidase N |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 647,941 | 0 | C | T | 37.0%
| 42.3
/ 41.2
| 70 | R207R (CGC→CGT) | pepN | aminopeptidase N |
| Reads supporting (aligned to +/- strand): ref base C (17/27); new base T (13/13); total (30/40) |
| Fisher's exact test for biased strand distribution p-value = 4.55e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.35e-01 |
TGTTTGCGCTGGTGGCAGGCGACTTTGATGTACTGCGCGATACCTTTACCACGCGTTCTGGTCGCGAAGTAGCACTGGAGCTGTACGTCGATCGCGGCAACCTTGATCGCGCGCCGTGGGCGATGA > minE/647877‑648002
|
tGTTTGCGCTGGTGGCAGGCGACTTTGATGTACTGCGCGATACCTTTACCACGCGTTCTGGTCGCGa > 1:289107/1‑67 (MQ=255)
tGTTTGCGCTGGTGGCAGGCGACTTTGATGTACTGCGCGATACCTTTACCACGCGTTCTGGTCGCGa > 1:1225786/1‑67 (MQ=255)
tGTTTGCGCTGGTGGCAGGCGACTTTGATGTACTGCGCGATACCTTTACCACGCGTTCTGGTCGCGa > 1:1310765/1‑67 (MQ=255)
tGCGCTGGTGGCAGGCGACTTTGATGTACTGCGCGATACCTTTACCACGCGTTCTGGTCGCGAAGTAg > 1:2184568/1‑68 (MQ=255)
tGCGCTGGTGGCAGGCGACTTTGATGTACTGCGCGATACCTTTACCACGCGTTCTGGTCGCGAAGTAGc < 1:2392034/69‑1 (MQ=255)
tGCGCTGGTGGCAGGCGACTTTGATGTACTGCGCGATACCTTTACCACGCGTTCTGGTCGCGAAGTAGc < 1:1737607/69‑1 (MQ=255)
tGCGCTGGTGGCAGGCGACTTTGATGTACTGCGCGATACCTTTACCACGCGTTCTGGTCGCGAAGTAGc < 1:2850298/69‑1 (MQ=255)
tGCGCTGGTGGCAGGCGACTTTGATGTACTGCGCGATACCTTTACCACGCGTTCTGGTCGCGAAGTAGc < 1:1555614/69‑1 (MQ=255)
tGCGCTGGTGGCAGGCGACTTTGATGTACTGCGCGATACCTTTACCACGCGTTCTGGTCGCGAAGTAGc < 1:2900456/69‑1 (MQ=255)
tGCGCTGGTGGCAGGCGACTTTGATGTACTGCGCGATACCTTTACCACGCGTTCTGGTCGCGAAGTAGc < 1:2151529/69‑1 (MQ=255)
tGCGCTGGTGGCAGGCGACTTTGATGTACTGCGCGATACCTTTACCACGCGTTCTGGTCGCGAAGTAGc < 1:1522304/69‑1 (MQ=255)
tGCGCTGGTGGCAGGCGACTTTGATGTACTGCGCGATACCTTTACCACGCGTTCTGGTCGCGAAGTAGc > 1:606134/1‑69 (MQ=255)
tGCGCTGGTGGCAGGCGACTTTGATGTACTGCGCGATACCTTTACCACGCGTTCTGGTCGCGAAGTAGc < 1:3264087/69‑1 (MQ=255)
tGCGCTGGTGGCAGGCGACTTTGATGTACTGCGCGATACCTTTACCACGCGTTCTGGTCGCGAAGTAGc < 1:3322482/69‑1 (MQ=255)
tGCGCTGGTGGCAGGCGACTTTGATGTACTGCGCGATACCTTTACCACGCGTTCTGGTCGCGAAGTAGc < 1:1149406/69‑1 (MQ=255)
tGCGCTGGTGGCAGGCGACTTTGATGTACTGCGCGATACCTTTACCACGCGTTCTGGTCGCGAAGTAGc < 1:1145068/69‑1 (MQ=255)
tGCGCTGGTGGCAGGCGACTTTGATGTACTGCGCGATACCTTTACCACGCGTTCTGGTCGCGAAGTAGc < 1:922613/69‑1 (MQ=255)
ttggtggCTGGTGATTTTGATGTACTGCGCGATACCTTCACTACCCGCTCTGGCCGTGAAGTGGCGCt < 1:597660/67‑1 (MQ=255)
ttggtggCTGGTGATTTTGATGTACTGCGCGATACCTTCACTACCCGCTCTGGCCGTGAAGTGGCGCt < 1:701460/67‑1 (MQ=255)
ttggtggCTGGTGATTTTGATGTACTGCGCGATACCTTCACTACCCGCTCTGGCCGTGAAGTGGCGCt < 1:2864050/67‑1 (MQ=255)
ttggtggCTGGTGATTTTGATGTACTGCGCGATACCTTCACTACCCGCTCTGGCCGTGAAGTGGCGCt < 1:1861711/67‑1 (MQ=255)
ttggtggCTGGTGATTTTGATGTACTGCGCGATACCTTCACTACCCGCTCTGGCCGTGAAGTGGCGCt < 1:1761963/67‑1 (MQ=255)
ttggtggCTGGTGATTTTGATGTACTGCGCGATACCTTCACTACCCGCTCTGGCCGTGAAGTGGCGCt < 1:1537979/67‑1 (MQ=255)
ttggtggCTGGTGATTTTGATGTACTGCGCGATACCTTCACTACCCGCTCTGGCCGTGAAGTGGCGCt < 1:742985/67‑1 (MQ=255)
ttggtggCTGGTGATTTTGATGTACTGCGCGATACCTTCACTACCCGCTCTGGCCGTGAAGTGGCGCt < 1:2906822/67‑1 (MQ=255)
ttggtggCTGGTGATTTTGATGTACTGCGCGATACCTTCACTACCCGCTCTGGCCGTGAAGTGGCGCt < 1:1297621/67‑1 (MQ=255)
ttggtggCTGGTGATTTTGATGTACTGCGCGATACCTTCACTACCCGCTCTGGCCGTGAAGTGGCGCt < 1:3386729/67‑1 (MQ=255)
ttggtggCTGGTGATTTTGATGTACTGCGCGATACCTTCACTAACCGCTCTGGCCGTGAAGTGGCGCt < 1:275477/67‑1 (MQ=255)
gtggtggCTGGTGATTTTGATGTACTGCGCGATACCTTCACTACCCGCTCTGGCCGTGAAGTGGCGCt < 1:342843/67‑1 (MQ=255)
ggtggCAGGCGACTTTGATGTACTGCGCGATACCTTTACCACGCGTTCTGGTCGCGAAGTAGCACTgg > 1:2622762/1‑68 (MQ=255)
ggtggCAGGCGACTTTGATGTACTGCGCGATACCTTTACCACGCGTTCTGGTCGCGAAGTAGCACTGGa > 1:2030282/1‑69 (MQ=255)
ggtggCAGGCGACTTTGATGTACTGCGCGATACCTTTACCACGCGTTCTGGTCGCGAAGTAGCACTGGa > 1:258749/1‑69 (MQ=255)
ggtggCAGGCGACTTTGATGTACTGCGCGATACCTTTACCACGCGTTCTGGTCGCGAAGTAGCACTGGa > 1:343062/1‑69 (MQ=255)
ggtggCAGGCGACTTTGATGTACTGCGCGATACCTTTACCACGCGTTCTGGTCGCGAAGTAGCACTGGa > 1:2484557/1‑69 (MQ=255)
ggtggCAGGCGACTTTGATGTACTGCGCGATACCTTTACCACGCGTTCTGGTCGCGAAGTAGCACTGGa > 1:1102925/1‑69 (MQ=255)
ggtggCAGGCGACTTTGATGTACTGCGCGATACCTTTACCACGCGTTCTGGTCGCGAAGTAGCACTGGa > 1:451210/1‑69 (MQ=255)
ggtggCAGGCGACTTTGATGTACTGCGCGATACCTTTACCACGCGTTCTGGTCGCGAAGTAGCACTGGa > 1:1248359/1‑69 (MQ=255)
ggtggCAGGCGACTTTGATGTACTGCGCGATACCTTTACCACGCGTTCTGGTCGCGAAGTAGCACTGGa > 1:2010221/1‑69 (MQ=255)
ggtggCAGGCGACTTTGATGTACTGCGCGATACCTTTACCACGCGTTCTGGTCGCGAAGTAGCACTGGa > 1:1467933/1‑69 (MQ=255)
ggtggCAGGCGACTTTGATGTACTGCGCGATACCTTTACCACGCGTTCTGGTCGCGAAGTAGCACTGGa > 1:1650299/1‑69 (MQ=255)
attttGATGTACTGCGCGATACCTTCACTACCCGCTCTGGCCGTGAAGTGGCGCt < 1:756278/53‑1 (MQ=255)
gcgcGATACCTTCACTACCCGCTCTGGCCGTGAAGTGGCGCTGGAGTTGTATGTCGATCGTGGCAAcc > 1:707418/1‑68 (MQ=255)
gcgcGATACCTTCACTACCCGCTCTGGCCGTGAAGTGGCGCTGGAGTTGTATGTCGATCGTGGCAAcc > 1:3330291/1‑68 (MQ=255)
gcgcGATACCTTCACTACCCGCTCTGGCCGTGAAGTGGCGCTGGAGTTGTATGTCGATCGTGGCAAcc > 1:2915786/1‑68 (MQ=255)
gcgcGATACCTTCACTACCCGCTCTGGCCGTGAAGTGGCGCTGGAGTTGTATGTCGATCGTGGCAAcc > 1:1549525/1‑68 (MQ=255)
gcgcGATACCTTCACTACCCGCTCTGGCCGTGAAGTGGCGCTGGAGTTGTATGTCGATCGTGGCAAcc > 1:1668907/1‑68 (MQ=255)
gcgcGATACCTTCACTACCCGCTCTGGCCGTGAAGTGGCGCTGGAGTTGTATGTCGATCGTGGCAAcc > 1:1712456/1‑68 (MQ=255)
gcgcGATACCTTCACTACCCGCTCTGGCCGTGAAGTGGCGCTGGAGTTGTATGTCGATCGTGGCAAcc > 1:2323823/1‑68 (MQ=255)
gcgcGATACCTTCACTACCCGCTCTGGCCGTGAAGTGGCGCTGGAGTTGTATGTCGATCGTGGCAAcc > 1:1869964/1‑68 (MQ=255)
gcgcGATACCTTCACTACCCGCTCTGGCCGTGAAGTGGCGCTGGAGTTGTATGTCGATCGTGGCAAcc > 1:1944714/1‑68 (MQ=255)
gcgcGATACCTTCACTACCCGCTCTGGCCGTGAAGTGGCGCTGGAGTTGTATGTCGATCGTGGCAAcc > 1:243221/1‑68 (MQ=255)
gcgcGATACCTTCACTACCCGCTCTGGCCGTGAAGTGGCGCTGGAGTTGTATGTCGATCGTGGCAAcc > 1:448900/1‑68 (MQ=255)
gcgcGATACCTTCACTACCCGCTCTGGCCGTGAACTGGCGCTGGAGTTGTATGTCGATCGTGGCaa > 1:2596225/1‑66 (MQ=255)
gcgcGATACCTTCACTACCCGCTATGGCCGTGAAGTGGCGCTGGAGTTGTATGTCGATCGTGGCAAcc > 1:2069169/1‑68 (MQ=255)
aCCTTTACCACGCGTTCTGGTCGCGAAGTAGCACTGGAGCTGTACGTCGATCTCGGCAACCtt < 1:236491/63‑1 (MQ=255)
aCCTTTACCACGCGTTCTGGTCGCGAAGTAGCACTGGAGCTGTACGTCGATCGCGGCAACCtt < 1:3387716/63‑1 (MQ=255)
aCCTTTACCACGCGTTCTGGTCGCGAAGTAGCACTGGAGCTGTACGTCGATCGCGGCAACCtt < 1:2703997/63‑1 (MQ=255)
aCCTTTACCACGCGTTCTGGTCGCGAAGTAGCACTGGAGCTGTACGTCGATCGCGGCAACCtt < 1:391927/63‑1 (MQ=255)
aCCTTTACCACGCGTTCTGGTCGCGAAGTAGCACTGGAGCTGTACGTCGATCGCGGCAACCtt < 1:1083147/63‑1 (MQ=255)
aCCTTTACCACGCGTTCTGGTCGCGAAGTAGCACTGGAGCTGTACGTCGATCGCGGCAACCtt < 1:2219895/63‑1 (MQ=255)
aCCTTTACCACGCGTTCTGGTCGCGAAGTAGCACTGGAGCTGTACGTCGATCGCGGCAACCtt < 1:2214016/63‑1 (MQ=255)
aCCTTTACCACGCGTTCTGGTCGCGAAGTAGCACTGGAGCTGTACGTCGATCGCGGCAACCtt < 1:612270/63‑1 (MQ=255)
aCCTTTACCACGCGTTCTGGTCGCGAAGTAGCACTGGAGCTGTACGTCGATCGCGGCAACCtt < 1:1898983/63‑1 (MQ=255)
aCCTTTACCACGCGTTCTGGTCGCGAAGTAGCACTGGAGCTGTACGTCGATCGCGGCAACCtt < 1:1867879/63‑1 (MQ=255)
aCCTTTACCACGCGTTCTGGTCGCGAAGTAGCACTGGAGCTGTACGTCGATCGCGGCAACCtt < 1:1470369/63‑1 (MQ=255)
aCCTTTACCACGCGTTCTGGTCGCGAAGTAGCACTGGAGCTGTACGTCGATCGCGGCAACCtt < 1:1383609/63‑1 (MQ=255)
aCCTTTACCACGCGTTCTGGTCGCGAAGTAGCACTGGAGCTGTACGTCGATCGCGGCAACCtt < 1:1132963/63‑1 (MQ=255)
aCCTTTACCACGCGTTCTGGTCGCGAAGTAGCACTGGAGCTGTACGTCGATCGCGGCAACCtt < 1:986495/63‑1 (MQ=255)
aCCTTTACCACGCGTTCTGGTCGCGAAGTAGCACTGGAGCTGTACGGCGATCGCGGCAACCtt < 1:2221256/63‑1 (MQ=255)
cTGGTCGCGAAGTAGCACTGGAGCTGTACGTCGATCGCGGCAACCTTGATCGCGCGCCGTGGGCGATGa > 1:2510132/1‑69 (MQ=255)
|
TGTTTGCGCTGGTGGCAGGCGACTTTGATGTACTGCGCGATACCTTTACCACGCGTTCTGGTCGCGAAGTAGCACTGGAGCTGTACGTCGATCGCGGCAACCTTGATCGCGCGCCGTGGGCGATGA > minE/647877‑648002
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
