Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
647,950 |
A→G |
45.6% |
A210A (GCA→GCG) |
pepN → |
aminopeptidase N |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 647,950 | 0 | A | G | 45.6%
| 5.2
/ 45.9
| 57 | A210A (GCA→GCG) | pepN | aminopeptidase N |
| Reads supporting (aligned to +/- strand): ref base A (13/18); new base G (13/13); total (26/31) |
| Fisher's exact test for biased strand distribution p-value = 6.00e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.18e-01 |
CTGGTGGCAGGCGACTTTGATGTACTGCGCGATACCTTTACCACGCGTTCTGGTCGCGAAGTAGCACTGGAGCTGTACGTCGATCGCGGCAACCTTGATCGCGCGCCGTGGGCGATGACCTCGCTGAAAAAC > minE/647885‑648016
|
ttggtggCTGGTGATTTTGATGTACTGCGCGATACCTTCACTACCCGCTCTGGCCGTGAAGTGGCGCt < 1:2864050/67‑1 (MQ=255)
ttggtggCTGGTGATTTTGATGTACTGCGCGATACCTTCACTACCCGCTCTGGCCGTGAAGTGGCGCt < 1:597660/67‑1 (MQ=255)
ttggtggCTGGTGATTTTGATGTACTGCGCGATACCTTCACTACCCGCTCTGGCCGTGAAGTGGCGCt < 1:1861711/67‑1 (MQ=255)
ttggtggCTGGTGATTTTGATGTACTGCGCGATACCTTCACTACCCGCTCTGGCCGTGAAGTGGCGCt < 1:1761963/67‑1 (MQ=255)
ttggtggCTGGTGATTTTGATGTACTGCGCGATACCTTCACTACCCGCTCTGGCCGTGAAGTGGCGCt < 1:3386729/67‑1 (MQ=255)
ttggtggCTGGTGATTTTGATGTACTGCGCGATACCTTCACTACCCGCTCTGGCCGTGAAGTGGCGCt < 1:1537979/67‑1 (MQ=255)
ttggtggCTGGTGATTTTGATGTACTGCGCGATACCTTCACTACCCGCTCTGGCCGTGAAGTGGCGCt < 1:701460/67‑1 (MQ=255)
ttggtggCTGGTGATTTTGATGTACTGCGCGATACCTTCACTACCCGCTCTGGCCGTGAAGTGGCGCt < 1:1297621/67‑1 (MQ=255)
ttggtggCTGGTGATTTTGATGTACTGCGCGATACCTTCACTACCCGCTCTGGCCGTGAAGTGGCGCt < 1:2906822/67‑1 (MQ=255)
ttggtggCTGGTGATTTTGATGTACTGCGCGATACCTTCACTACCCGCTCTGGCCGTGAAGTGGCGCt < 1:742985/67‑1 (MQ=255)
ttggtggCTGGTGATTTTGATGTACTGCGCGATACCTTCACTAACCGCTCTGGCCGTGAAGTGGCGCt < 1:275477/67‑1 (MQ=255)
gtggtggCTGGTGATTTTGATGTACTGCGCGATACCTTCACTACCCGCTCTGGCCGTGAAGTGGCGCt < 1:342843/67‑1 (MQ=255)
ggtggCAGGCGACTTTGATGTACTGCGCGATACCTTTACCACGCGTTCTGGTCGCGAAGTAGCACTgg > 1:2622762/1‑68 (MQ=255)
ggtggCAGGCGACTTTGATGTACTGCGCGATACCTTTACCACGCGTTCTGGTCGCGAAGTAGCACTGGa > 1:258749/1‑69 (MQ=255)
ggtggCAGGCGACTTTGATGTACTGCGCGATACCTTTACCACGCGTTCTGGTCGCGAAGTAGCACTGGa > 1:2484557/1‑69 (MQ=255)
ggtggCAGGCGACTTTGATGTACTGCGCGATACCTTTACCACGCGTTCTGGTCGCGAAGTAGCACTGGa > 1:343062/1‑69 (MQ=255)
ggtggCAGGCGACTTTGATGTACTGCGCGATACCTTTACCACGCGTTCTGGTCGCGAAGTAGCACTGGa > 1:1102925/1‑69 (MQ=255)
ggtggCAGGCGACTTTGATGTACTGCGCGATACCTTTACCACGCGTTCTGGTCGCGAAGTAGCACTGGa > 1:2030282/1‑69 (MQ=255)
ggtggCAGGCGACTTTGATGTACTGCGCGATACCTTTACCACGCGTTCTGGTCGCGAAGTAGCACTGGa > 1:2010221/1‑69 (MQ=255)
ggtggCAGGCGACTTTGATGTACTGCGCGATACCTTTACCACGCGTTCTGGTCGCGAAGTAGCACTGGa > 1:451210/1‑69 (MQ=255)
ggtggCAGGCGACTTTGATGTACTGCGCGATACCTTTACCACGCGTTCTGGTCGCGAAGTAGCACTGGa > 1:1248359/1‑69 (MQ=255)
ggtggCAGGCGACTTTGATGTACTGCGCGATACCTTTACCACGCGTTCTGGTCGCGAAGTAGCACTGGa > 1:1467933/1‑69 (MQ=255)
ggtggCAGGCGACTTTGATGTACTGCGCGATACCTTTACCACGCGTTCTGGTCGCGAAGTAGCACTGGa > 1:1650299/1‑69 (MQ=255)
attttGATGTACTGCGCGATACCTTCACTACCCGCTCTGGCCGTGAAGTGGCGCt < 1:756278/53‑1 (MQ=255)
gcgcGATACCTTCACTACCCGCTCTGGCCGTGAAGTGGCGCTGGAGTTGTATGTCGATCGTGGCAAcc > 1:448900/1‑68 (MQ=255)
gcgcGATACCTTCACTACCCGCTCTGGCCGTGAAGTGGCGCTGGAGTTGTATGTCGATCGTGGCAAcc > 1:707418/1‑68 (MQ=255)
gcgcGATACCTTCACTACCCGCTCTGGCCGTGAAGTGGCGCTGGAGTTGTATGTCGATCGTGGCAAcc > 1:2915786/1‑68 (MQ=255)
gcgcGATACCTTCACTACCCGCTCTGGCCGTGAAGTGGCGCTGGAGTTGTATGTCGATCGTGGCAAcc > 1:1549525/1‑68 (MQ=255)
gcgcGATACCTTCACTACCCGCTCTGGCCGTGAAGTGGCGCTGGAGTTGTATGTCGATCGTGGCAAcc > 1:243221/1‑68 (MQ=255)
gcgcGATACCTTCACTACCCGCTCTGGCCGTGAAGTGGCGCTGGAGTTGTATGTCGATCGTGGCAAcc > 1:3330291/1‑68 (MQ=255)
gcgcGATACCTTCACTACCCGCTCTGGCCGTGAAGTGGCGCTGGAGTTGTATGTCGATCGTGGCAAcc > 1:2323823/1‑68 (MQ=255)
gcgcGATACCTTCACTACCCGCTCTGGCCGTGAAGTGGCGCTGGAGTTGTATGTCGATCGTGGCAAcc > 1:1668907/1‑68 (MQ=255)
gcgcGATACCTTCACTACCCGCTCTGGCCGTGAAGTGGCGCTGGAGTTGTATGTCGATCGTGGCAAcc > 1:1712456/1‑68 (MQ=255)
gcgcGATACCTTCACTACCCGCTCTGGCCGTGAAGTGGCGCTGGAGTTGTATGTCGATCGTGGCAAcc > 1:1869964/1‑68 (MQ=255)
gcgcGATACCTTCACTACCCGCTCTGGCCGTGAAGTGGCGCTGGAGTTGTATGTCGATCGTGGCAAcc > 1:1944714/1‑68 (MQ=255)
gcgcGATACCTTCACTACCCGCTCTGGCCGTGAACTGGCGCTGGAGTTGTATGTCGATCGTGGCaa > 1:2596225/1‑66 (MQ=255)
gcgcGATACCTTCACTACCCGCTATGGCCGTGAAGTGGCGCTGGAGTTGTATGTCGATCGTGGCAAcc > 1:2069169/1‑68 (MQ=255)
aCCTTTACCACGCGTTCTGGTCGCGAAGTAGCACTGGAGCTGTACGTCGATCTCGGCAACCtt < 1:236491/63‑1 (MQ=255)
aCCTTTACCACGCGTTCTGGTCGCGAAGTAGCACTGGAGCTGTACGTCGATCGCGGCAACCtt < 1:1898983/63‑1 (MQ=255)
aCCTTTACCACGCGTTCTGGTCGCGAAGTAGCACTGGAGCTGTACGTCGATCGCGGCAACCtt < 1:986495/63‑1 (MQ=255)
aCCTTTACCACGCGTTCTGGTCGCGAAGTAGCACTGGAGCTGTACGTCGATCGCGGCAACCtt < 1:1132963/63‑1 (MQ=255)
aCCTTTACCACGCGTTCTGGTCGCGAAGTAGCACTGGAGCTGTACGTCGATCGCGGCAACCtt < 1:1383609/63‑1 (MQ=255)
aCCTTTACCACGCGTTCTGGTCGCGAAGTAGCACTGGAGCTGTACGTCGATCGCGGCAACCtt < 1:1470369/63‑1 (MQ=255)
aCCTTTACCACGCGTTCTGGTCGCGAAGTAGCACTGGAGCTGTACGTCGATCGCGGCAACCtt < 1:612270/63‑1 (MQ=255)
aCCTTTACCACGCGTTCTGGTCGCGAAGTAGCACTGGAGCTGTACGTCGATCGCGGCAACCtt < 1:1867879/63‑1 (MQ=255)
aCCTTTACCACGCGTTCTGGTCGCGAAGTAGCACTGGAGCTGTACGTCGATCGCGGCAACCtt < 1:2703997/63‑1 (MQ=255)
aCCTTTACCACGCGTTCTGGTCGCGAAGTAGCACTGGAGCTGTACGTCGATCGCGGCAACCtt < 1:391927/63‑1 (MQ=255)
aCCTTTACCACGCGTTCTGGTCGCGAAGTAGCACTGGAGCTGTACGTCGATCGCGGCAACCtt < 1:2214016/63‑1 (MQ=255)
aCCTTTACCACGCGTTCTGGTCGCGAAGTAGCACTGGAGCTGTACGTCGATCGCGGCAACCtt < 1:2219895/63‑1 (MQ=255)
aCCTTTACCACGCGTTCTGGTCGCGAAGTAGCACTGGAGCTGTACGTCGATCGCGGCAACCtt < 1:3387716/63‑1 (MQ=255)
aCCTTTACCACGCGTTCTGGTCGCGAAGTAGCACTGGAGCTGTACGTCGATCGCGGCAACCtt < 1:1083147/63‑1 (MQ=255)
aCCTTTACCACGCGTTCTGGTCGCGAAGTAGCACTGGAGCTGTACGGCGATCGCGGCAACCtt < 1:2221256/63‑1 (MQ=255)
cTGGTCGCGAAGTAGCACTGGAGCTGTACGTCGATCGCGGCAACCTTGATCGCGCGCCGTGGGCGATGa > 1:2510132/1‑69 (MQ=255)
aGTAGCACTGGAGCTGTACGTCGATCGCGGCAACCTTGATCGCGCGCCGTGGGCGATGACCTCGCTGaa < 1:197465/69‑1 (MQ=255)
gTAGCACTGGAGCTGTACGTCGATCGCGGCAACCTTGATCGCGCGCCGTGGGCGATGACCTCGCTGaaa < 1:1191898/69‑1 (MQ=255)
tAGCACTGGAGCTGTACGTCGATCGCGGCAACCTTGATCGCGCGCCGTGGGCGATGACCTCGCTGaaa < 1:721454/68‑1 (MQ=255)
gCACTGGAGCTGTACGTCGATCGCGGCAACCTTGATCGCGCGCCGTGGGCGATGACCTCGCTGAAAAAc > 1:858410/1‑69 (MQ=255)
|
CTGGTGGCAGGCGACTTTGATGTACTGCGCGATACCTTTACCACGCGTTCTGGTCGCGAAGTAGCACTGGAGCTGTACGTCGATCGCGGCAACCTTGATCGCGCGCCGTGGGCGATGACCTCGCTGAAAAAC > minE/647885‑648016
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
