Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
649,288 |
A→G |
48.4% |
L656L (CTA→CTG) |
pepN → |
aminopeptidase N |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 649,288 | 0 | A | G | 48.4%
| ‑3.2
/ 54.8
| 62 | L656L (CTA→CTG) | pepN | aminopeptidase N |
| Reads supporting (aligned to +/- strand): ref base A (12/20); new base G (14/16); total (26/36) |
| Fisher's exact test for biased strand distribution p-value = 6.07e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CCGATTGCTATTGCCGAAGTACGCGAAGCACTCACTCGTACTCTGGCGACTGAACTGGCGGATGAGCTACTGGCTATTTACAACGCGAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACG > minE/649220‑649353
|
ccGATTGCTATTGCCGAAGTACGCGAAGCACTCACTCGTACTCTGGCGACTGAACTGGCGGATGAGCTa > 1:3089956/1‑69 (MQ=255)
ccGATTGCTATTGCCGAAGTACGCGAAGCACTCACTCGTACTCTGGCGACTGAACTGGCGGATGAGCTa < 1:1787745/69‑1 (MQ=255)
ccGATTGCTATTGCCGAAGTACGCGAAGCACTCACTCGTACTCTGGCGACTGAACTGGCGGATGAGCTa > 1:1384253/1‑69 (MQ=255)
ccGATTGCTATTGCCGAAGTACGCGAAGCACTCACTCGTACTCTGGCGACTGAACTGGCGGATGAGCTa > 1:1256431/1‑69 (MQ=255)
ccGATTGCTATTGCCGAAGTACGCGAAGCACTCAATCGTACTCTGGCGACTGAACTGGCGGATGAGCTa > 1:1746206/1‑69 (MQ=255)
gCCGAAGTACGCGAAGCACTCCCTCGTACTCTGGCGACTGAACTGGCAGATGAGCTGCTGGCTATTTAc > 1:2375520/1‑69 (MQ=255)
gCCGAAGTACGCGAAGCACTCACTCGTACTCTGTCGACTGAACTGGCAGATGAGCTGCTGGCTATTTAc > 1:777253/1‑69 (MQ=255)
gCCGAAGTACGCGAAGCACTCACTCGTACTCTGGCGACTGAACTGGCAGATGAGCTGCTGGCTATTTAc > 1:1081225/1‑69 (MQ=255)
gCCGAAGTACGCGAAGCACTCACTCGTACTCTGGCGACTGAACTGGCAGATGAGCTGCTGGCTATTTAc > 1:2371486/1‑69 (MQ=255)
gCCGAAGTACGCGAAGCACTCACTCGTACTCTGGCGACTGAACTGGCAGATGAGCTGCTGGCTATTTAc > 1:1781457/1‑69 (MQ=255)
gCCGAAGTACGCGAAGCACTCACTCGTACTCTGGCGACTGAACTGGCAGATGAGCTGCTGGCTATTTAc > 1:511292/1‑69 (MQ=255)
gCCGAAGTACGCGAAGCACTCACTCGTACTCTGGCGACTGAACTGGCAGATGAGCTGCTGGCTATTTAc > 1:1731785/1‑69 (MQ=255)
gCCGAAGTACGCGAAGCACTCACTCGTACTCTGGCGACTGAACTGGCAGATGAGCTGCTGGCTATTTAc > 1:1687972/1‑69 (MQ=255)
gCCGAAGTACGCGAAGCACTCACTCGTACTCTGGCGACTGAACTGGCAGATGAGCTGCTGGCTATTTAc > 1:2697056/1‑69 (MQ=255)
gCCGAAGTACGCGAAGCACTCACTCGTACTCTGGCGACTGAACTGGCAGATGAGCTGCTGGCTATTTAc > 1:2700809/1‑69 (MQ=255)
gCCGAAGTACGCGAAGCACTCACTCGTACTCTGGCGACTGAACTGGCAGATGAGCTGCTGGCTATTTAc > 1:1939681/1‑69 (MQ=255)
gCCGAAGTACGCGAAGCACTCACTCGTACTCTGGCGACTGAACTGGCAGATGAGCTGCTGGCTATTTAc > 1:3314504/1‑69 (MQ=255)
gCCGAAGTACGCGAAGCACTCACTCGTACTCTGGCGACTGAACTGGCAGATGAGCTGCTGGCTATTTAc > 1:2929220/1‑69 (MQ=255)
gCCGAAGTACGCGAAGCACTAACTCGTACTCTGGCGACTGAACTGGCAGATGAGCTGCTGGCTATTTAc > 1:1170248/1‑69 (MQ=255)
gAAGTACGCGAAGCACTCACTCGTACTCTGGCGACTGAACTGGCGGATGAGCTACTGGCTATTTACAAc < 1:1209284/69‑1 (MQ=255)
gTACGCGAAGCACTCACTCGTACTCTGGCGACTGAACTGGCGGATGAGCTACTGGCTATTTACAAcgcg < 1:1241995/69‑1 (MQ=255)
gTACGCGAAGCACTCACTCGTACTCTGGCGACTGAACTGGCGGATGAGCTACTGGCTATTTACAAcgcg < 1:72064/69‑1 (MQ=255)
cgcgAAGCACTCACTCGTACTCTGGCGACTGAACTGGCGGATGAGCTACTGGCTATTTACAACGCGAAt > 1:2768018/1‑69 (MQ=255)
cgcgAAGCACTCACTCGTACTCTGGCGACTGAACTGGCGGATGAGCTACTGGCTATTTACAACGCGAAt > 1:2025204/1‑69 (MQ=255)
gAAGCACTCACTCGTACTCTGGCGACTGAACTGGCGGATGAGCTACTGGCTATTTACAACGCGAATTAc < 1:3148309/69‑1 (MQ=255)
gCACTCACTCGTACTCTGGCGACTGAACTGGCAGATGAGCTGCTGGCTATTTACAACGCGAATTACCag < 1:2122453/69‑1 (MQ=255)
gCACTCACTCGTACTCTGGCGACTGAACTGGCAGATGAGCTGCTGGCTATTTACAACGCGAATTACCag < 1:3156675/69‑1 (MQ=255)
gCACTCACTCGTACTCTGGCGACTGAACTGGCAGATGAGCTGCTGGCTATTTACAACGCGAATTACCag < 1:2879092/69‑1 (MQ=255)
gCACTCACTCGTACTCTGGCGACTGAACTGGCAGATGAGCTGCTGGCTATTTACAACGCGAATTACCag < 1:2822558/69‑1 (MQ=255)
gCACTCACTCGTACTCTGGCGACTGAACTGGCAGATGAGCTGCTGGCTATTTACAACGCGAATTACCag < 1:27993/69‑1 (MQ=255)
gCACTCACTCGTACTCTGGCGACTGAACTGGCAGATGAGCTGCTGGCTATTTACAACGCGAATTACCag < 1:3154873/69‑1 (MQ=255)
gCACTCACTCGTACTCTGGCGACTGAACTGGCAGATGAGCTGCTGGCTATTTACAACGCGAATTACCag < 1:411763/69‑1 (MQ=255)
gCACTCACTCGTACTCTGGCGACTGAACTGGCAGATGAGCTGCTGGCTATTTACAACGCGAATTACCag < 1:2684228/69‑1 (MQ=255)
gCACTCACTCGTACTCTGGCGACTGAACTGGCAGATGAGCTGCTGGCTATTTACAACGCGAATTACCag < 1:2572033/69‑1 (MQ=255)
gCACTCACTCGTACTCTGGCGACTGAACTGGCAGATGAGCTGCTGGCTATTTACAACGCGAATTACCag < 1:742896/69‑1 (MQ=255)
gCACTCACTCGTACTCTGGCGACTGAACTGGCAGATGAGCTGCTGGCTATTTACAACGCGAATTACCag < 1:529008/69‑1 (MQ=255)
gCACTCACTCGTACTCTGGCGACTGAACTGGCAGATGAGCTGCTGGCTATTTACAACGCGAATTACCag < 1:1000810/69‑1 (MQ=255)
gCACTCACTCGTACTCTGGCGACTGAACTGGCAGATGAGCTGCTGGCTATTTACAACGCGAATTACCag < 1:700612/69‑1 (MQ=255)
cactcactCGTACTCTGGCGACTGAACTGGCAGATGAGCTGCTGGCTATTTACAACGCGAATTACCag < 1:2602838/68‑1 (MQ=255)
cactcactCGTACTCTGGCGACTGAACTGGCAGATGAGCTGCTGGCTATTTACAACGCGAATTACCag < 1:1350446/68‑1 (MQ=255)
cactcactCGTACTCTGGCGACTGAACTGGCAGATGAGCTGCTGGCTATTTACAACGCGAATTACCag < 1:2983182/68‑1 (MQ=255)
tcGTACTCTGGCGACTGAACTGGCGGATGAGCTACTGGTTATTTACAACGCGAATTACCAGAGCGAGTa < 1:1477375/69‑1 (MQ=255)
tcGTACTCTGGCGACTGAACTGGCGGATGAGCTACTGGCTATTTACAACGCGAATTACCAGAGCGAGTa < 1:2162719/69‑1 (MQ=255)
tcGTACTCTGGCGACTGAACTGGCGGATGAGCTACTGGCTATTTACAACGCGAATTACCAGAGCGAGTa < 1:1304859/69‑1 (MQ=255)
tcGTACTCTGGCGACTGAACTGGCGGATGAGCTACTGGCTATTTACAACGCGAATTACCAGAGCGAGTa < 1:1753305/69‑1 (MQ=255)
cGTACTCTGGCGACTGAACTGGCGGATGAGCTACTGGCTATTTACAACGCGAATTACCAGAGCGAGTa > 1:1364016/1‑68 (MQ=255)
cGTACTCTGGCGACTGAACTGGCGGATGAGCTACTGGCTATTTACAACGCGAATTACCAGAGCGAGTAc > 1:890897/1‑69 (MQ=255)
cGTACTCTGGCGACTGAACTGGCGGATGAGCTACTGGCTATTTACAACGCGAATTACCAGAGCGAGTAc > 1:3324481/1‑69 (MQ=255)
cGTACTCTGGCGACTGAACTGGCGGATGAGCTACTGGCTATTTACAACGCGAATTACCAGAGCGAGTAc > 1:954750/1‑69 (MQ=255)
cGTACTCTGGCGACTGAACTGGCGGATGAGCTACTGGCTATTTACAACGCGAATTACCAGAGCGAGTAc > 1:2700229/1‑69 (MQ=255)
cGTACTCTGGCGACTGAACTGGCGGATGAGCTACTGGCTATTTACAACGCGAATTACCAGAGCGAGTAc > 1:2444096/1‑69 (MQ=255)
cGTACTCTGGCGACTGAACTGGCGGATGAGCTACTGGCTATTTACAACGCGAATTACCAGAGCGAGTAc > 1:1752492/1‑69 (MQ=255)
cTGGCGGATGAGCTACTGGCTATTTACAACGCGAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGat > 1:2892202/1‑69 (MQ=255)
cTGGCGGATGAGCTACTGGCTATTTACAACGCGAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGat > 1:2880047/1‑69 (MQ=255)
cTGGCGGATGAGCTACTGGCTATTTACAACGCGAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGat > 1:2843020/1‑69 (MQ=255)
cTGGCGGATGAGCTACTGGCTATTTACAACGCGAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGa < 1:1300499/68‑1 (MQ=255)
cTACTGGCTATTTACAACGCGAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACg < 1:1073718/68‑1 (MQ=255)
cTACTGGCTATTTACAACGCGAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACg < 1:3002906/68‑1 (MQ=255)
cTACTGGCTATTTACAACGCGAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACg < 1:917501/68‑1 (MQ=255)
cTACTGGCTATTTACAACGCGAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACg < 1:1269571/68‑1 (MQ=255)
cTACTGGCTATTTACAACGCGAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACg < 1:1878557/68‑1 (MQ=255)
cTACTGGCTATTTACAACGCGAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACg < 1:1902361/68‑1 (MQ=255)
cTACTGGCTATTTACAACGCGAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACg < 1:630019/68‑1 (MQ=255)
cTACTGGCTATTTACAACGCGAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACg < 1:2415569/68‑1 (MQ=255)
cTACTGGCTATTTACAACGCGAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACg < 1:520776/68‑1 (MQ=255)
cTACTGGCTATTTACAACGCGAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACg < 1:3290174/68‑1 (MQ=255)
cTACTGGCTATTTACAACGCGAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACg < 1:3219924/68‑1 (MQ=255)
|
CCGATTGCTATTGCCGAAGTACGCGAAGCACTCACTCGTACTCTGGCGACTGAACTGGCGGATGAGCTACTGGCTATTTACAACGCGAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACG > minE/649220‑649353
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
