Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
694,020 |
G→T |
24.0% |
T191T (ACG→ACT) |
hyaF → |
protein involved in nickel incorporation into hydrogenase‑1 proteins |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 694,020 | 0 | G | T | 24.0%
| 137.0
/ 32.2
| 79 | T191T (ACG→ACT) | hyaF | protein involved in nickel incorporation into hydrogenase‑1 proteins |
| Reads supporting (aligned to +/- strand): ref base G (33/27); new base T (5/14); total (38/41) |
| Fisher's exact test for biased strand distribution p-value = 3.67e-02 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.45e-02 |
GTTGGCGCATGAGTTACTGGCACATGTACGTAACCCCGACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCACGTCTCTGTGGGCCG > minE/693954‑694074
|
gTTGGCGCATGAGTTACTGGCACATGTACGTAACCCCGACGCGCAGCCGCACAGCATTAATCTTACGc < 1:47364/68‑1 (MQ=255)
gTTGGCGCATGAGTTACTGGCACATGTACGTAACCCCGACGCGCAGCCGCACAGCATTAATCTGACGc < 1:1152764/68‑1 (MQ=255)
gTTGGCGCATGAGTTACTGGCACATGTACGTAACCCCGACGCGCAGCCGCACAGCATTAATCTGACGc < 1:664998/68‑1 (MQ=255)
gTTGGCGCATGAGTTACTGGCACATGTACGTAACCCCGACGCGCAGCCGCACAGCATTAATCTGACGc < 1:2350345/68‑1 (MQ=255)
gTTGGCGCATGAGTTACTGGCACATGTACGTAACCCCGACGCGCAGCCGCACAGCATTAATCTGACGc < 1:1505152/68‑1 (MQ=255)
gTTGGCGCATGAGTTACTGGCACATGTACGTAACCCCGACGCGCAGCCGCACAGCATTAATCTGACGc < 1:2949983/68‑1 (MQ=255)
ggagcATGAGTTACTGGCACATGTACGTAACCCCGACGCGCAGCCGCACAGCATTAATCTGACGc < 1:2983252/62‑1 (MQ=255)
ggCGCATGAGTTACTGGCACATGTACGTAACCCCGACGCGCAGCCGCACAGCATTAATCTGACGc < 1:886221/65‑1 (MQ=255)
ggCGCATGAGTTACTGGCACATGTACGTAACCCCGACGCGCAGCCGCACAGCATTAATCTGACGc < 1:2015469/65‑1 (MQ=255)
ggCGCATGAGTTACTGGCACATGTACGTAACCCCGACGCGCAGCCGCACAGCATTAATCTGACGc < 1:1764768/65‑1 (MQ=255)
ggCGCATGAGTTACTGGCACATGTACGTAACCCCGACGCGCAGCCGCACAGCATTAATCTGACGc < 1:2191243/65‑1 (MQ=255)
ggCGCATGAGTTACTGGCACATGTACGTAACCCCGACGCGCAGCCGCACAGCATTAATCTGACGc < 1:2855356/65‑1 (MQ=255)
ggCGCATGAGTTACTGGCACATGTACGTAACCCCGACGCGCAGCCGCACAGCATTAATCTGACGc < 1:2939093/65‑1 (MQ=255)
ggCGCATGAGTTACTGGCACATGTACGTAACCCCGACGCGCAGCCGCACAGCATTAATCTGACGc < 1:1346896/65‑1 (MQ=255)
ggCGCATGAGTTACTGGCACATGTACGTAACCCCGACGCGCAGCCGCACAGCATTAATCTGACGCAAtt > 1:464029/1‑69 (MQ=255)
gTACGTAACCCCGACGCGCAGCCGCACAGCATTAATCTGACTCAATTACCCATCAGCGAAGCTGACCgg < 1:3324717/69‑1 (MQ=255)
gTACGTAACCCCGACGCGCAGCCGCACAGCATTAATCTGACTCAATTACCCATCAGCGAAGCTGACCgg < 1:250439/69‑1 (MQ=255)
gTACGTAACCCCGACGCGCAGCCGCACAGCATTAATCTGACTCAATTACCCATCAGCGAAGCTGACCgg < 1:2362975/69‑1 (MQ=255)
gTACGTAACCCCGACGCGCAGCCGCACAGCATTAATCTGACTCAATTACCCATCAGCGAAGCTGACCgg < 1:586782/69‑1 (MQ=255)
gTACGTAACCCCGACGCGCAGCCGCACAGCATTAATCTGACTCAATTACCCATCAGCGAAGCTGACCgg < 1:719847/69‑1 (MQ=255)
gTACGTAACCCCGACGCGCAGCCGCACAGCATTAATCTGACTCAATTACCCATCAGCGAAGCTGACCgg < 1:868518/69‑1 (MQ=255)
gTACGTAACCCCGACGCGCAGCCGCACAGCATTAATCTGACTCAATTACCCATCAGCGAAGCTGACCgg < 1:1139349/69‑1 (MQ=255)
gTACGTAACCCCGACGCGCAGCCGCACAGCATTAATCTGACTCAATTACCCATCAGCGAAGCTGACCgg < 1:1910631/69‑1 (MQ=255)
gTACGTAACCCCGACGCGCAGCCGCACAGCATTAATCTGACTCAATTACCCATCAGCGAAGCTGACCgg < 1:1861363/69‑1 (MQ=255)
gTACGTAACCCCGACGCGCAGCCGCACAGCATTAATCTGACTCAATTACCCATCAGCGAAGCTGACCgg < 1:1860381/69‑1 (MQ=255)
gTACGTAACCCCGACGCGCAGCCGCACAGCATTAATCTGACTCAATTACCCATCAGCGAAGCTGACCgg < 1:1334089/69‑1 (MQ=255)
gTACGTAACCCCGACGCGCAGCCGCACAGCATTAATCTGACTCAATTACCCATCAGCGAAGCTGACCgg < 1:1211086/69‑1 (MQ=255)
gTACGTAACCCCGACGCGCAGCCGCACAGCATTAATCTGACTCAATTACCCATCAGCGAAGCTGACCgg < 1:1785991/69‑1 (MQ=255)
tACGTAACCCCGACGCGCAGCCGCACAGCATTAATCTGACTCAATTACCCATCAGCGAAGCTGACCgg < 1:2811832/68‑1 (MQ=255)
aaCCCCGACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCttttt > 1:1970939/1‑69 (MQ=255)
aaCCCCGACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCttttt > 1:3364214/1‑69 (MQ=255)
aaCCCCGACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCttttt > 1:3314201/1‑69 (MQ=255)
aaCCCCGACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCttttt > 1:3092119/1‑69 (MQ=255)
aaCCCCGACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCttttt > 1:2993736/1‑69 (MQ=255)
aaCCCCGACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCttttt > 1:1330084/1‑69 (MQ=255)
aaCCCCGACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCttttt > 1:2877106/1‑69 (MQ=255)
ccGACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTtctct > 1:3274832/1‑69 (MQ=255)
ccGACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTtctct > 1:3427225/1‑69 (MQ=255)
ccGACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTtctct > 1:3004169/1‑69 (MQ=255)
ccGACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTtctct > 1:1388050/1‑69 (MQ=255)
ccGACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTtctct > 1:2826724/1‑69 (MQ=255)
ccGACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTtctct > 1:130072/1‑69 (MQ=255)
ccGACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTtctct > 1:890571/1‑69 (MQ=255)
ccGACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTtctct > 1:1902930/1‑69 (MQ=255)
ccGACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTtctct > 1:2376249/1‑69 (MQ=255)
ccGACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTtctct > 1:1088841/1‑69 (MQ=255)
ccGACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTtctct > 1:2328235/1‑69 (MQ=255)
ccGACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTtctct > 1:2151829/1‑69 (MQ=255)
ccGACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTtctct > 1:2155610/1‑69 (MQ=255)
gACGCGCAGCCGCACATCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCa < 1:360955/69‑1 (MQ=255)
gACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCa < 1:848753/69‑1 (MQ=255)
gACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCa < 1:400596/69‑1 (MQ=255)
gACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCa < 1:3331295/69‑1 (MQ=255)
gACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCa < 1:1144259/69‑1 (MQ=255)
gACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCa < 1:3007748/69‑1 (MQ=255)
gACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCa < 1:1760756/69‑1 (MQ=255)
gACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCa < 1:1373623/69‑1 (MQ=255)
gACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCa < 1:2326417/69‑1 (MQ=255)
gACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCa < 1:1726148/69‑1 (MQ=255)
gACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCa < 1:2674086/69‑1 (MQ=255)
gACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCa < 1:2598218/69‑1 (MQ=255)
gACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCa < 1:2417633/69‑1 (MQ=255)
gCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCACGTctct > 1:162879/1‑68 (MQ=255)
gCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCACGTCTCtg > 1:747118/1‑69 (MQ=255)
gCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCACGTCTCtg > 1:2140024/1‑69 (MQ=255)
gCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCACGTCTCtg > 1:2056288/1‑69 (MQ=255)
gCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCACGTCTCtg > 1:1714688/1‑69 (MQ=255)
gCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCACGTCTCtg > 1:2642852/1‑69 (MQ=255)
gCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCACGTCTCtg > 1:1885879/1‑69 (MQ=255)
gCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCACGTCTCTGTGGGCCg > 1:1293087/1‑68 (MQ=255)
gCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCACGTCTCTGTGGGCCg > 1:15786/1‑68 (MQ=255)
gCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCACGTCTCTGTGGGCCg > 1:3199430/1‑68 (MQ=255)
gCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCACGTCTCTGTGGGCCg > 1:3121724/1‑68 (MQ=255)
gCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCACGTCTCTGTGGGCCg > 1:1635813/1‑68 (MQ=255)
cATTAATCTGACTCAATTACCCATCAGCGAAGCTGACCGGCTTTTtctc > 1:1364616/1‑49 (MQ=37)
cATTAATCTGACTCAATTACCCATCAGCGAAGCTGACCGGCTTTTtctc > 1:83642/1‑49 (MQ=37)
cATTAATCTGACTCAATTACCCATCAGCGAAGCTGACCGGCTTTTtctc > 1:2076962/1‑49 (MQ=37)
cATTAATCTGACTCAATTACCCATCAGCGAAGCTGACCGGCTTTTtctc > 1:1859793/1‑49 (MQ=37)
cATTAATCTGACTCAATTACCCATCAGCGAAGCTGACCGGCTTTTtctc > 1:1875369/1‑49 (MQ=37)
|
GTTGGCGCATGAGTTACTGGCACATGTACGTAACCCCGACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCACGTCTCTGTGGGCCG > minE/693954‑694074
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
