Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
893,980 |
G→A |
56.2% |
L149L (CTG→TTG) |
sapB ← |
predicted antimicrobial peptide transporter subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 893,980 | 0 | G | A | 56.2%
| 15.0
/ 56.4
| 73 | L149L (CTG→TTG) | sapB | predicted antimicrobial peptide transporter subunit |
| Reads supporting (aligned to +/- strand): ref base G (28/4); new base A (34/7); total (62/11) |
| Fisher's exact test for biased strand distribution p-value = 7.46e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01 |
CCATGGTGAGTCCGAAAGCCAGGCATCAATCAACGCAAAACCGGTAATCGGTTTCACTTCGTAGAGCAGATCGAAACGCCCTGAAACGGGCAACCAACCTAGCGTGAGTGAACAAAACAGCGTCAAC > minE/893912‑894038
|
ccATGGTGAGTCCGAAAGCCAGGCATCAATCAACGCAAAACCGGTAATCGGTTTCACTTCGTAGAGCAg < 1:911870/69‑1 (MQ=255)
ccATGGTGAGTCCGAAAGCCAGGCATCAATCAACGCAAAACCGGTAATCGGTTTCACTTCGTAGAGCAg < 1:3091752/69‑1 (MQ=255)
ccATGGTGAGTCCGAAAGCCAGGCATCAATCAACGCAAAACCGGTAATCGGTTTCACTTCGTAGAGCAg < 1:3105682/69‑1 (MQ=255)
ccATGGTGAGTCCGAAAGCCAGGCATCAATCAACGCAAAACCGGTAATCGGTTTCACTTCGTAGAGCAg < 1:2932527/69‑1 (MQ=255)
ccATGGTGAGTCCGAAAGCCAGGCATCAATCAACGCAAAACCGGTAATCGGTTTCACTTCGTAGAGCAg < 1:2897472/69‑1 (MQ=255)
ccATGGTGAGTCCGAAAGCCAGGCATCAATCAACGCAAAACCGGTAATCGGTTTCACTTCGTAGAGCAg < 1:283688/69‑1 (MQ=255)
ccATGGTGAGTCCGAAAGCCAGGCATCAATCAACGCAAAACCGGTAATCGGTTTCACTTCGTAGAGCAg < 1:2720287/69‑1 (MQ=255)
ccATGGTGAGTCCGAAAGCCAGGCATCAATCAACGCAAAACCGGTAATCGGTTTCACTTCGTAGAGCAg < 1:3289028/69‑1 (MQ=255)
ccATGGTGAGTCCGAAAGCCAGGCATCAATCAACGCAAAACCGGTAATCGGTTTCACTTCGTAGAGCAg < 1:2254880/69‑1 (MQ=255)
ccATGGTGAGTCCGAAAGCCAGGCATCAATCAACGCAAAACCGGTAATCGGTTTCACTTCGTAGAGCAg < 1:3433091/69‑1 (MQ=255)
ccATGGTGAGTCCGAAAGCCAGGCATCAATCAACGCAAAACCGGTAATCGGTTTCACTTCGTAGAGCAg < 1:402429/69‑1 (MQ=255)
ccATGGTGAGTCCGAAAGCCAGGCATCAATCAACGCAAAACCGGTAATCGGTTTCACTTCGTAGAGCAg < 1:404972/69‑1 (MQ=255)
ccATGGTGAGTCCGAAAGCCAGGCATCAATCAACGCAAAACCGGTAATCGGTTTCACTTCGTAGAGCAg < 1:1479098/69‑1 (MQ=255)
ccATGGTGAGTCCGAAAGCCAGGCATCAATCAACGCAAAACCGGTAATCGGTTTCACTTCGTAGAGCAg < 1:141325/69‑1 (MQ=255)
gTCCGAAAGCCAGGCATCAATCAACGCAAAACCGGTAATCGGTTTCACTTCGTAGAGCAg < 1:818282/60‑1 (MQ=255)
tCCGAAAGCCAGGCATCAATCAACGCAAAACCGGTAATCGGTTTCACTTCGTAGAGCAGATCGAAACGc > 1:1319028/1‑69 (MQ=255)
aaaGCCAGGCATCAATCAACGCAAAACCGGTAATCGGTTTCACTTCGTAGAGCAGATCGAAACGc > 1:2198649/1‑65 (MQ=255)
aaaGCCAGGCATCAATCAACGCAAAACCGGTAATCGGTTTCACTTCGTAGAGCAGATCGAAACGc > 1:746709/1‑65 (MQ=255)
gCCAGGCATCAATCAACGCAAAACCGGTAATCGGTTTCACTTCGTAGAGCAGATCGAAACGCCCTGaaa > 1:1056882/1‑69 (MQ=255)
gCCAGGCATCAATCAACGCAAAACCGGTAATCGGTTTCACTTCGTAGAGCAGATCGAAACGCCCTGaaa > 1:2639708/1‑69 (MQ=255)
gCCAGGCATCAATCAACGCAAAACCGGTAATCGGTTTCACTTCGTAGAGCAGATCGAAACGCCCTGaaa > 1:1765922/1‑69 (MQ=255)
gCCAGGCATCAATCAACGCAAAACCGGTAATCGGTTTCACTTCGTAGAGCAAATCGAAACGCCCAGaaa < 1:140004/69‑1 (MQ=255)
gCCAGGCATCAATCAACGCAAAACCGGTAATCGGTTTCACTTCGTAGAGCAAATCGAAACGCCCAGaaa < 1:3373560/69‑1 (MQ=255)
gCCAGGCATCAATCAACGCAAAACCGGTAATCGGTTTCACTTCGTAGAGCAAATCGAAACGCCCAGaaa < 1:426177/69‑1 (MQ=255)
gCCAGGCATCAATCAACGCAAAACCGGTAATCGGTTTCACTTCGTAGAGCAAATCGAAACGCCCAGaaa < 1:1212219/69‑1 (MQ=255)
gCCAGGCATCAATCAACGCAAAACCGGTAATCGGTTTCACTTCGTAGAGCAAATCGAAACGCCCAGaaa < 1:3141029/69‑1 (MQ=255)
gCCAGGCATCAATCAACGCAAAACCGGTAATCGGTTTCACTTCGTAGAGCAAATCGAAACGCCCAGaaa < 1:2009602/69‑1 (MQ=255)
cAGGCATCAATCAACGCAAAACCGGTAATCGGTTTCACTTCGTAGAGCAGATCGAAACGCCCTGAAACg > 1:2435216/1‑69 (MQ=255)
aGGCATCAATCAACGCAAAACCGGTAATCGGTTTCACTTCTTAGAGCAAATCGAAACGCCCAGaaa < 1:1512895/66‑1 (MQ=255)
tcaatcaaCGGAAAACCGGTAATCGGTTTCACTTCGTAGAGCAGATCGAAACGCCCTGAAACGGGcaa > 1:2001492/1‑68 (MQ=255)
tcaatcaaCGCAAAACCGGTAATCGGTTTCACTTCGTAGAGCAGATCGGAACGCCCTGAAACGGGcaa > 1:2536368/1‑68 (MQ=255)
tcaatcaaCGCAAAACCGGTAATCGGTTTCACTTCGTAGAGCAGATCGAAACGCCCTGAAACGGGcaa > 1:1169620/1‑68 (MQ=255)
tcaatcaaCGCAAAACCGGTAATCGGTTTCACTTCGTAGAGCAGATCGAAACGCCCTGAAACGGGcaa > 1:1156423/1‑68 (MQ=255)
tcaatcaaCGCAAAACCGGTAATCGGTTTCACTTCGTAGAGCAGATCGAAACGCCCTGAAACGGGcaa > 1:3139734/1‑68 (MQ=255)
tcaatcaaCGCAAAACCGGTAATCGGTTTCACTTCGTAGAGCAGATCGAAACGCCCTGAAACGGGcaa > 1:2682367/1‑68 (MQ=255)
tcaatcaaCGCAAAACCGGTAATCGGTTTCACTTCGTAGAGCAGATCGAAACGCCCTGAAACGGGcaa > 1:1085407/1‑68 (MQ=255)
tcaatcaaCGCAAAACCGGTAATCGGTTTCACTTCGTAGAGCAGATCGAAACGCCCTGAAACGGGcaa > 1:2944552/1‑68 (MQ=255)
tcaatcaaCGCAAAACCGGTAATCGGTTTCACTTCGTAGAGCAGATCGAAACGCCCTGAAACGGGcaa > 1:2990936/1‑68 (MQ=255)
tcaatcaaCGCAAAACCGGTAATCGGTTTCACTTCGTAGAGCAGATCGAAACGCCCTGAAACGGGcaa > 1:147319/1‑68 (MQ=255)
tcaatcaaCGCAAAACCGGTAATCGGTTTCACTTCGTAGAGCAGATCGAAACGCCCTGAAACGGGcaa > 1:2599396/1‑68 (MQ=255)
tcaatcaaCGCAAAACCGGTAATCGGTTTCACTTCGTAGAGCAGATCGAAACGCCCTGAAACGGGcaa > 1:1263510/1‑68 (MQ=255)
tcaatcaaCGCAAAACCGGTAATCGGTTTCACTTCGTAGAGCAGATCGAAACGCCCTGAAACGGGcaa > 1:397689/1‑68 (MQ=255)
tcaatcaaCGCAAAACCGGTAATCGGTTTCACTTCGTAGAGCAGATCGAAACGCCCTGAAACGGGcaa > 1:593209/1‑68 (MQ=255)
tcaatcaaCGCAAAACCGGTAATCGGTTTCACTTCGTAGAGCAGATCGAAACGCCCTGAAACGGGcaa > 1:1546313/1‑68 (MQ=255)
tcaatcaaCGCAAAACCGGTAATCGGTTTCACTTCGTAGAGAAGATCGAAACGccc > 1:3246481/1‑56 (MQ=255)
aatcaaCGCAAAACCGGTAATCGGTTTCACTTCGTAGAGCAAATCg > 1:2402836/1‑46 (MQ=255)
aatcaaCGCAAAACCGGTAATCGGTTTCACTTCGTAGAGCAAATCg > 1:620898/1‑46 (MQ=255)
aatcaaCGCAAAACCGGTAATCGGTTTCACTTCGTAGAGCAAATCGa > 1:3340581/1‑47 (MQ=255)
aatcaaCGCAAAACCGGTAATCGGTTTCACTTCGTAGAGCAAATCGa > 1:368749/1‑47 (MQ=255)
aatcaaCGCAAAACCGGTAATCGGTTTCACTTCGTAGAGCAAATCGa > 1:390034/1‑47 (MQ=255)
aatcaaCGCAAAACCGGTAATCGGTTTCACTTCGTAGAGCAAATCGa > 1:723497/1‑47 (MQ=255)
aatcaaCGCAAAACCGGTAATCGGTTTCACTTCGTAGAGCAAATCGa > 1:568268/1‑47 (MQ=255)
aatcaaCGCAAAACCGGTAATCGGTTTCACTTCGTAGAGCAAATCGa > 1:60618/1‑47 (MQ=255)
aatcaaCGCAAAACCGGTAATCGGTTTCACTTCGTAGAGCAAATCGa > 1:3111664/1‑47 (MQ=255)
aatcaaCGCAAAACCGGTAATCGGTTTCACTTCGTAGAGCAAATCGa > 1:2730567/1‑47 (MQ=255)
aatcaaCGCAAAACCGGTAATCGGTTTCACTTCGTAGAGCAAATCGa > 1:3102611/1‑47 (MQ=255)
aatcaaCGCAAAACCGGTAATCGGTTTCACTTCGTAGAGCAAATCGa > 1:1952717/1‑47 (MQ=255)
aatcaaCGCAAAACCGGTAATCGGTTTCACTTCGTAGAGCAAATCGa > 1:2018467/1‑47 (MQ=255)
aatcaaCGCAAAACCGGTAATCGGTTTCACTTCGTAGAGCAAATCGa > 1:158146/1‑47 (MQ=255)
aatcaaCGCAAAACCGGTAATCGGTTTCACTTCGTAGAGCAAATCGa > 1:234049/1‑47 (MQ=255)
aatcaaCGCAAAACCGGTAATCGGTTTCACTTCGTAGAGCAAATCGa > 1:2617349/1‑47 (MQ=255)
aatcaaCGCAAAACCGGTAATCGGTTTCACTTCGTAGAGCAAATCGa > 1:1351899/1‑47 (MQ=255)
aatcaaCGCAAAACCGGTAATCGGTTTCACTTCGTAGAGCAAATCGa > 1:2810396/1‑47 (MQ=255)
aatcaaCGCAAAACCGGTAATCGGTTTCACTTCGTAGAGCAAATCGa > 1:2919360/1‑47 (MQ=255)
aatcaaCGCAAAACCGGTAATCGGTTTCACTTCGTAGAGCAAATCGa > 1:1542813/1‑47 (MQ=255)
aatcaaCGCAAAACCGCTAATCGGTTTCACTTCGTAGAGCAAATCGa > 1:336841/1‑47 (MQ=255)
tcaaCGCAACACCGGTAATCGGTTTCACTTCGTAGAGCAGATCGAAACGc < 1:126961/50‑1 (MQ=255)
aaCGCAAAACCGGTAATCGGTTTCACTTCGTAGAGCAGATCGAAACGCCCTGAAACGGGCAACCAACCt > 1:1704022/1‑69 (MQ=255)
aaCGCAAAACCGGTAATCGGTTTCACTTCGTAGAGCAAATc > 1:1941974/1‑41 (MQ=255)
aaCGCAAAACCGGTAATCGGTTTCACTTCGTAGAGCAAATCGAAACGCCCAGAAACg > 1:1563862/1‑57 (MQ=255)
aaCGCAAAACCGGTAATCGGTTTCACTTCGTAGAGCAAATCGAAACGCCCAGAAACg > 1:1566140/1‑57 (MQ=255)
aaCGCAAAACCGGTAATCGGTTTCACTTCGTAGAGCAAATCGAAACGCCCAGAAACg > 1:430284/1‑57 (MQ=255)
aaCGCAAAACCGGTAATCGGTTTCACTTCGTAGAGCAAATCGAAACGCCCAGAAACg > 1:640432/1‑57 (MQ=255)
aaCGCAAAACCGGTAATCGGTTTCACTTCGTAGAGCAAATCGAAACGCCCAGAAACg > 1:1998914/1‑57 (MQ=255)
aaCGCAAAACCGGTAATCGGTTTCACTTCGTAGAGCAAATCGAAACGCCCAGAAACg > 1:2048613/1‑57 (MQ=255)
aaCGCAAAACCGGTAATCGGTTTCACTTCGTAGAGCAAATCGAAACGCCCAGAAACg > 1:2569411/1‑57 (MQ=255)
aaCGCAAAACCGGTAATCGGTTTCACTTCGTAGAGCAAATCGAAACGCCCAGAAACg > 1:3337582/1‑57 (MQ=255)
aaCGCAAAACCGGTAATCGGTTTCACTTCGTAGAGCAAATCGAAACGCCCAGAAACg > 1:751245/1‑57 (MQ=255)
aaCGCAAAACCGGTAATCGGTTTCACTTCGTAGAGCAAATCGAAACGCCCAGAAACg > 1:3218815/1‑57 (MQ=255)
aaCGCAAAACCGGTAATCGGTTTCACTTCGTAGAGCAAATCGAAACGCCCAGAAACg > 1:2949504/1‑57 (MQ=255)
aaCGCAAAACCGGTAATCGGTTTCACTTCGTAGAGCAAATCGAAACGCACAGAAACg > 1:3377857/1‑57 (MQ=255)
cGCAAAACCGGTAATCGGTTTCACTTCGTAGAGCAGATCGAAACGCCCTGAAACGGGCAACCAACCTAg > 1:123739/1‑69 (MQ=255)
cGCAAAACCGGTAATCGGTTTCACTTCGTAGAGCAGATCGAAACGCCCTGAAACGGGCAACCAACCTAg > 1:294989/1‑69 (MQ=255)
cGCAAAACCGGTAATCGGTTTCACTTCGTAGAGCAGATCGAAACGCCCTGAAACGGGCAACCAACCTAg > 1:3292564/1‑69 (MQ=255)
aaTCGGTTTCACTTCGTAGAGCAGATCGAAACGCCCTGAAAC‑GGCAACCAACCTAGCGTGAGTGAACaa < 1:1548042/69‑1 (MQ=255)
aaTCGGTTTCACTTCGTAGAGCAGATCGAAACGCCCTGAAAC‑GGCAACCAACCTAGCGTGAGTGAACaa < 1:519095/69‑1 (MQ=255)
aaTCGGTTTCACTTCGTAGAGCAGATCGAAACGCCCTGAAAC‑GGCAACCAACCTAGCGTGAGTGAACaa < 1:1019194/69‑1 (MQ=255)
cGTAGAGCAGATCGAAACGCCCTGAAACGGGCAACCAACCTAGCGTGAGTGAACAAAACAGCGTCAAc > 1:1000152/1‑68 (MQ=255)
|
CCATGGTGAGTCCGAAAGCCAGGCATCAATCAACGCAAAACCGGTAATCGGTTTCACTTCGTAGAGCAGATCGAAACGCCCTGAAACGGGCAACCAACCTAGCGTGAGTGAACAAAACAGCGTCAAC > minE/893912‑894038
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
