Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
920,323 |
A→G |
38.9% |
R92R (CGT→CGC) |
mpaA ← |
murein peptide amidase A |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 920,323 | 0 | A | G | 38.9%
| 34.3
/ 51.7
| 77 | R92R (CGT→CGC) | mpaA | murein peptide amidase A |
| Reads supporting (aligned to +/- strand): ref base A (21/26); new base G (13/17); total (34/43) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.61e-01 |
CCATTAGCATTGGCCCGTAACCCCAACTGGCAGCCGTCAGGATTCACACACAGCACCACATGATGACGGCGCAAAGAAGGTGTCAACGTCCGCAGAGCACAGGAGAGCGTGACGACCGAAGAGTTTTCATC > minE/920258‑920388
|
ccATTAGCATTGGCCCGTAACCCTAACTGGCAGCCGTCAGGATTCACACACAGCACCACATGATGGCgg < 1:2164305/69‑1 (MQ=255)
ccATTAGCATTGGCCCGTAACCCTAACTGGCAGCCGTCAGGATTCACACACAGCACCACATGATGGCgg < 1:2194438/69‑1 (MQ=255)
ccATTAGCATTGGCCCGTAACCCTAACTGGCAGCCGTCAGGATTCACACACAGCACCACATGATGGCgg < 1:476617/69‑1 (MQ=255)
ccATTAGCATTGGCCCGTAACCCTAACTGGCAGCCGTCAGGATTCACACACAGCACCACATGATGGCgg < 1:3432658/69‑1 (MQ=255)
ccATTAGCATTGGCCCGTAACCCTAACTGGCAGCCGTCAGGATTCACACACAGCACCACATGATGGCgg < 1:324947/69‑1 (MQ=255)
ccATTAGCATTGGCCCGTAACCCTAACTGGCAGCCGTCAGGATTCACACACAGCACCACATGATGGCgg < 1:2782723/69‑1 (MQ=255)
cATTAGCATTGGCCCGTAACCCTAACTGGCAGCCGTCAGGATTCACACACAGCACCACATGATGGCgg < 1:3189570/68‑1 (MQ=255)
cATTAGCATTGGCCCGTAACCCCAACTGGCAGCCGTCAGGATTCACACACAGCACCACATGATGGCgg < 1:413081/68‑1 (MQ=255)
ttGGCCCGTAACCCCAACTGGCAGCCGTCAGGATTCACACACAGCACCACATGATGACGGCGCAaagaa < 1:3395955/69‑1 (MQ=255)
ttGGCCCGTAACCCCAACTGGCAGCCGTCAGGATTCACACACAGCACCACATGATGACGGCGCAaagaa > 1:208983/1‑69 (MQ=255)
ttGGCCCGTAACCCCAACTGGCAGCCGTCAGGATTCACACACAGCACCACATGATGACGGCGCAaagaa < 1:3108140/69‑1 (MQ=255)
ttGGCCCGTAACCCCAACTGGCAGCCGTCAGGATTCACACACAGCACCACATGATGACGGCGCAaagaa > 1:3231695/1‑69 (MQ=255)
ttGGCCCGTAACCCCAACTGGCAGCCGTCAGGATTCACACACAGCACCACATGATGACGGCGCAaagaa < 1:189931/69‑1 (MQ=255)
ttGGCCCGTAACCCCAACTGGCAGCCGTCAGGATTCACACACAGCACCACATGATGACGGCGCAaagaa > 1:2207564/1‑69 (MQ=255)
ttGGCCCGTAACCCCAACTGGCAGCCGTCAGGATTCACACACAGCACCACATGATGACGGCGCAaagaa > 1:1788082/1‑69 (MQ=255)
ttGGCCCGTAACCCCAACTGGCAGCCGTCAGGATTCACACACAGCACCACATGATGACGGCGCAaagaa > 1:3403504/1‑69 (MQ=255)
ttGGCCCGTAACCCCAACTGGCAGCCGTCAGGATTCACACACAGCACCACATGATGACGGCGCAaagaa > 1:1340749/1‑69 (MQ=255)
ttGGCCCGTAACCCCAACTGGCAGCCGTCAGGATTCACACACAGCACCACATGATGACGGCGCAaagaa > 1:2326075/1‑69 (MQ=255)
ttGGCCCGTAACCCCAACTGGCAGCCGTCAGGATTCACACACAGCACCACATGATGACGGCGCAaagaa > 1:637091/1‑69 (MQ=255)
ttGGCCCGTAACCCCAACTGGCAGCCGTCAGGATTCACACACAGCACCACATGATGACGGCGCAaagaa < 1:2331819/69‑1 (MQ=255)
cGTAACCCCAACTGTCAGCCGTCAGGATTCACACACAGCACCACATGATGACGGCGCAAAGAAGGTGTc > 1:559290/1‑69 (MQ=255)
cGTAACCCCAACTGGCAGCCGTCAGGATTCACACACAGCACCACATGATGACGGCGCAAAGAAGGTGTc > 1:2676900/1‑69 (MQ=255)
cGTAACCCCAACTGGCAGCCGTCAGGATTCACACACAGCACCACATGATGACGGCGCAAAGAAGGTGTc > 1:2686259/1‑69 (MQ=255)
cGTAACCCCAACTGGCAGCCGTCAGGATTCACACACAGCACCACATGATGACGGCGCAAAGAAGGTGTc > 1:299942/1‑69 (MQ=255)
cGTAACCCCAACTGGCAGCCGTCAGGATTCACACACAGCACCACATGATGACGGCGCAAAGAAGGTGTc > 1:1827228/1‑69 (MQ=255)
cGTAACCCCAACTGGCAGCCGTCAGGATTCACACACAGCACCACATGATGACGGCGCAAAGAAGGTGTc > 1:1825369/1‑69 (MQ=255)
cGTAACCCCAACTGGCAGCCGTCAGGATTCACACACAGCACCACATGATGACGGCGCAAAGAAGGTGTc > 1:1179222/1‑69 (MQ=255)
gTAACCCTAACTGGCAGCCGTGAGGATTCACACACAGCACCACATGATGGCGGCGCAAAGACGgtgt > 1:2081149/1‑67 (MQ=255)
gTAACCCTAACTGGCAGCCGTCAGGATTCACACACAGCACCACATGATGGCGGCGCAAAGACGgtgt > 1:2891522/1‑67 (MQ=255)
gTAACCCTAACTGGCAGCCGTCAGGATTCACACACAGCACCACATGATGGCGGCGCAAAGACGgtgt > 1:2873168/1‑67 (MQ=255)
gTAACCCTAACTGGCAGCCGTCAGGATTCACACACAGCACCACATGATGGCGGCGCAAAGACGgtgt > 1:2787902/1‑67 (MQ=255)
gTAACCCTAACTGGCAGCCGTCAGGATTCACACACAGCACCACATGATGGCGGCGCAAAGACGgtgt > 1:2761532/1‑67 (MQ=255)
gTAACCCTAACTGGCAGCCGTCAGGATTCACACACAGCACCACATGATGGCGGCGCAAAGACGgtgt > 1:2726594/1‑67 (MQ=255)
gTAACCCTAACTGGCAGCCGTCAGGATTCACACACAGCACCACATGATGGCGGCGCAAAGACGgtgt > 1:409116/1‑67 (MQ=255)
gTAACCCTAACTGGCAGCCGTCAGGATTCACACACAGCACCACATGATGGCGGCGCAAAGACGgtgt > 1:941457/1‑67 (MQ=255)
gTAACCCTAACTGGCAGCCGTCAGGATTCACACACAGCACCACATGATGGCGGCGCAAAGACGgtgt > 1:1084515/1‑67 (MQ=255)
gTAACCCTAACTGGCAGCCGTCAGGATTCACACACAGCACCACATGATGGCGGCGCAAAGACGgtgt > 1:1039746/1‑67 (MQ=255)
gTAACCCTAACTGGCAGCCGTCAGGATTCACACACAGCACCACATGATGGCGGCGCAAAGACGgtgt > 1:1463846/1‑67 (MQ=255)
gTAACCCTAACTGGCAGCCGTCAGGATTCACACACAGCACCACATGATGGCGGCGCAAAGACGgtgt > 1:1131508/1‑67 (MQ=255)
gTAACCCTAACTGGCAGCCGTCAGGATTCACACACAGCACCACATGATGGCGGCGCAAAGACGgtgt > 1:2322887/1‑67 (MQ=255)
gTAACCCTAACTGGCAGCCGTCAGGATTCACACACAGCACCACATGATGGCGGCGCAAAGACGGTGTCa < 1:8260/69‑1 (MQ=255)
gTAACCCTAACTGGCAGCCGTCAGGATTCACACACAGCACCACATGATGGCGGCGCAAAGACGGTGTCa < 1:1090285/69‑1 (MQ=255)
gTAACCCTAACTGGCAGCCGTCAGGATTCACACACAGCACCACATGATGGCGGCGCAAAGACGGTGTCa < 1:2311809/69‑1 (MQ=255)
gTAACCCTAACTGGCAGCCGTCAGGATTCACACACAGCACCACATGATGGCGGCGCAAAGACGGTGTCa < 1:907838/69‑1 (MQ=255)
gTAACCCTAACTGGCAGCCGTCAGGATTCACACACAGCACCACATGATGGCGGCGCAAAGACGGTGTCa < 1:1193843/69‑1 (MQ=255)
gTAACCCTAACTGGCAGCCGTCAGGATTCACACACAGCACCACATGATGGCGGCGCAAAGACGGTGTCa < 1:2223533/69‑1 (MQ=255)
gTAACCCTAACTGGCAGCCGTCAGGATTCACACACAGCACCACATGATGGCGGCGCAAAGACGGTGTCa < 1:2198039/69‑1 (MQ=255)
gTAACCCTAACTGGCAGCCGTCAGGATTCACACACAGCACCACATGATGGCGGCGCAAAGACGGTGTCa < 1:3247688/69‑1 (MQ=255)
gTAACCCTAACTGGCAGCCGTCAGGATTCACACACAGCACCAAATGATGgcggcg > 1:377522/1‑55 (MQ=255)
aCCCCAACTGGCAGCCGTCAGGATTCACACACAGCACCACATGATGACGGCGCAAAGAAGGTGTCAACg > 1:66984/1‑69 (MQ=255)
aCCCCAACTGGCAGCCGTCAGGATTCACACACAGCACCACATGATGACGGCGCAAAGAAGGTGTCAACg > 1:3312060/1‑69 (MQ=255)
ggCAGCCGTCAGGATTCACACACAGCACCACATGATGGCGGCGCAAAGACGGTGTCa < 1:2128245/57‑1 (MQ=255)
aTTCACACACAGCACCACATGATGACGGCGCAAAGAAGGTGTCAACGTCCGCAGAGCACAGGAGAGCGt > 1:2889312/1‑69 (MQ=255)
cacacacaGCACCACATGATGACGGCGCAAAGAAGGTGTCAACGTCCGCAGAGCACAGGAGAGCGTgac < 1:1092537/69‑1 (MQ=255)
cacacacaGCACCACATGATGACGGCGCAAAGAAGGTGTCAACGTCCGCAGAGCACAGGAGAGCGTgac < 1:984432/69‑1 (MQ=255)
cacacacaGCACCACATGATGACGGCGCAAAGAAGGTGTCAACGTCCGCAGAGCACAGGAGAGCGTgac < 1:2270645/69‑1 (MQ=255)
cacacacaGCACCACATGATGACGGCGCAAAGAAGGTGTCAACGTCCGCAGAGCACAGGAGAGCGTgac < 1:2182803/69‑1 (MQ=255)
cacaGCACCACATGATGACGGCGCAAAGAAGGTGTCAACGTc > 1:1381504/1‑42 (MQ=255)
cacaGCACCACATGATGACGGCGCAAAGAAGGTGTCAACGTCCGCAGAGCACAGgag > 1:957548/1‑57 (MQ=255)
cacaGCACCACATGATGACGGCGCAAAGAAGGTGTCAACGTCCGCAGAGCACAGgag > 1:19283/1‑57 (MQ=255)
caGCACCACATGATGACGGCGCAAAGAAGGTGTCAACGTCCGCAGAGCACAGgag < 1:2416600/55‑1 (MQ=255)
caGCACCACATGATGACGGCGCAAAGAAGGTGTCAACGTCCGCAGAGCACAGgag < 1:2026814/55‑1 (MQ=255)
caGCACCACATGATGACGGCGCAAAGAAGGTGTCAACGTCCGCAGAGCACAGgag < 1:947623/55‑1 (MQ=255)
caGCACCACATGATGACGGCGCAAAGAAGGTGTCAACGTCCGCAGAGCACAGgag < 1:897877/55‑1 (MQ=255)
caGCACCACATGATGACGGCGCAAAGAAGGTGTCAACGTCCGCAGAGCACAGgag < 1:873882/55‑1 (MQ=255)
caGCACCACATGATGACGGCGCAAAGAAGGTGTCAACGTCCGCAGAGCACAGgag < 1:2656712/55‑1 (MQ=255)
caGCACCACATGATGACGGCGCAAAGAAGGTGTCAACGTCCGCAGAGCACAGgag < 1:647559/55‑1 (MQ=255)
caGCACCACATGATGACGGCGCAAAGAAGGTGTCAACGTCCGCAGAGCACAGgag < 1:2322666/55‑1 (MQ=255)
caGCACCACATGATGACGGCGCAAAGAAGGTGTCAACGTCCGCAGAGCACAGgag < 1:488169/55‑1 (MQ=255)
caGCACCACATGATGACGGCGCAAAGAAGGTGTCAACGTCCGCAGAGCACAGgag < 1:2764197/55‑1 (MQ=255)
caGCACCACATGATGACGGCGCAAAGAAGGTGTCAACGTCCGCAGAGCACAGgag < 1:3000402/55‑1 (MQ=255)
caGCACCACATGATGACGGCGCAAAGAAGGTGTCAACGTCCGCAGAGCACAGgag < 1:1545411/55‑1 (MQ=255)
caGCACCACATGATGACGGCGCAAAGAAGGTGTCAACGTCCGCAGAGCACAGgag < 1:3336908/55‑1 (MQ=255)
caGCACCACATGATGACGGCGCAAAGAAGGTGTCAACGTCCGCAGAGCACAGgag < 1:3073732/55‑1 (MQ=255)
caGCACCACATGATGACGGCGCAAAGAAGGTGTCAACGTCCGCAGAGCACAGgag < 1:3234850/55‑1 (MQ=255)
caGCACCACATGATGACGGCGCAAAGAAGGTGTCAACGTCCGCAGAGCACAGgag < 1:1949809/55‑1 (MQ=255)
caGCACCACATGATGACGGCGCAAAGAAGGTGTCAACGTCCGCAGAGCACAGgag < 1:2005543/55‑1 (MQ=255)
aGCACCACATGATGACGGCGCAAAGACGGTGTCAACGTCCGCAGAGCACAGgag < 1:3205112/54‑1 (MQ=255)
atgaCGGCGCAAAGAAGGTGTCAACGTCCGTAGAGCACAGGAGAGCGTGACGACCGAAGAGTTTtcatc > 1:1442475/1‑69 (MQ=255)
atgaCGGCGCAAAGAAGGTGTCAACGTCCGTAGAGCACAGGAGAGCGTGACGACCGAAGAGTTTtcatc > 1:3316518/1‑69 (MQ=255)
atgaCGGCGCAAAGAAGGTGTCAACGTCCGTAGAGCACAGGAGAGCGTGACGACCGAAGAGTTTtcatc > 1:2377338/1‑69 (MQ=255)
atgaCGGCGCAAAGAAGGTGTCAACGTCCGCAGAGca < 1:2740209/37‑1 (MQ=255)
atgaCGGCGCAAAGAAGGTGTCAACGTCCGCAGAGca < 1:307818/37‑1 (MQ=255)
atgaCGGCGCAAAGAAGGTGTCAACGTCCGCAGAGca < 1:2644549/37‑1 (MQ=255)
atgaCGGCGCAAAGAAGGTGTCAACGTCCGCAGAGca < 1:2568126/37‑1 (MQ=255)
|
CCATTAGCATTGGCCCGTAACCCCAACTGGCAGCCGTCAGGATTCACACACAGCACCACATGATGACGGCGCAAAGAAGGTGTCAACGTCCGCAGAGCACAGGAGAGCGTGACGACCGAAGAGTTTTCATC > minE/920258‑920388
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
