Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,026,083 |
G→C |
46.2% |
L263L (CTC→CTG) |
malI ← |
DNA‑binding transcriptional repressor |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,026,083 | 0 | G | C | 46.2%
| 14.6
/ 92.8
| 80 | L263L (CTC→CTG) | malI | DNA‑binding transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base G (4/39); new base C (15/22); total (19/61) |
| Fisher's exact test for biased strand distribution p-value = 1.41e-03 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01 |
GCCGCCAGCGAAACCTGTTGCTCAAAGTAACGATCGACTCCGCTTTCCCCGCTTTGCCGCCCTGCTTTGAGCAAACCAAACCATGCCCCCATCGCAATAGTTTCGTTATAGCAAACCACGGCACTGATG > minE/1026015‑1026143
|
gccgccAGCGAAACCTGTTGCTCAAAGTAACGATCGACTCCGCTTTCCCCGCTTTGCCTCCCTGCTTTg > 1:3303347/1‑69 (MQ=255)
gccgccAGCGAAACCTGTTGCTCAAAGTAACGATCGACTCCGCTTTCCCCGCTTTGCCGCCCTGCTTTg > 1:75721/1‑69 (MQ=255)
gccgccAGCGAAACCTGTTGCTCAAAGTAACGATCGACTCCGCTTTCCCCGCTTTGCCGCCCTGCTTTg < 1:1600038/69‑1 (MQ=255)
aaaCCTGTTGCTCAAAGTAACGATCGACTCCGCTTTCCCCGCTTTGCCGCCCTGCTTTGAGcaaaccaa < 1:755598/69‑1 (MQ=255)
aaaCCTGTTGCTCAAAGTAACGATCGACTCCGCTTTCCCCGCTTTGCCGCCCTGCTTTGAGcaaaccaa < 1:3213800/69‑1 (MQ=255)
aaaCCTGTTGCTCAAAGTAACGATCGACTCCGCTTTCCCCGCTTTGCCGCCCTGCTTTGAGcaaaccaa < 1:198734/69‑1 (MQ=255)
aaaCCTGTTGCTCAAAGTAACGATCGACTCCGCTTTCCCCGCTTTGCCGCCCTGCTTTGAGcaaaccaa < 1:44896/69‑1 (MQ=255)
aaaCCTGTTGCTCAAAGTAACGATCGACTCCGCTTTCCCCGCTTTGCCGCCCTGCTTTGAGcaaaccaa < 1:3107372/69‑1 (MQ=255)
aaaCCTGTTGCTCAAAGTAACGATCGACTCCGCTTTCCCCGCTTTGCCGCCCTGCTTTGAGcaaaccaa < 1:789576/69‑1 (MQ=255)
aaaCCTGTTGCTCAAAGTAACGATCGACTCCGCTTTCCCCGCTTTGCCGCCCTGCTTTGAGcaaaccaa < 1:970617/69‑1 (MQ=255)
aaaCCTGTTGCTCAAAGTAACGATCGACTCCGCTTTCCCCGCTTTGCCGCCCTGCTTTGAGcaaaccaa < 1:940835/69‑1 (MQ=255)
aaCCTGTTGCTCAAAGTAACGATCGACTCCGCTTTCCCCGCTTTGCCGCCCTGCTTTGAGcaaaccaa < 1:1598474/68‑1 (MQ=255)
aaCCTGTTGCTCAAAGTAACGATCGACTCCGCTTTCCCCGCTTTGCC‑CCCTGCTTTGAGcaaaccaaac < 1:1375301/69‑1 (MQ=255)
aaCCTGTTGCGCAAAGTAACGATCGACTCCGCTTTCCCCGCTTTGCCGCCCTGCTTTGAGcaaaccaa < 1:96283/68‑1 (MQ=255)
aCCTGTTGCTCAAAGTAACGATCGACTCCGCTTTCCCCGCTTTGCCGCCCTGCTTTGAGcaaaccaaac < 1:1699981/69‑1 (MQ=255)
aCCTGTTGCTCAAAGTAACGATCGACTCCGCTTTCCCCGCTTTGCCGCCCTGCTTTGAGcaaaccaaac < 1:1762204/69‑1 (MQ=255)
aCCTGTTGCTCAAAGTAACGATCGACTCCGCTTTCCCCGCTTTGCCGCCCTGCTTTGAGcaaaccaaac < 1:2909621/69‑1 (MQ=255)
gTAACGATCGACTCCGCTTTCCCCGCTTTGCCGCCCTGCTTTGAGCAAACCAAACCATGcccc > 1:1309378/1‑63 (MQ=255)
gTAACGATCGACTCCGCTTTCCCCGCTTTGCCGCCCTGCTTTGAGCAAACCAAACCATGCCCCCATca > 1:1527524/1‑67 (MQ=255)
gTAACGATCGACTCCGCTTTCCCCGCTTTGCCGCCCTGCTTTGAGCAAACCAAACCATGCCCCCATCGc > 1:3159497/1‑69 (MQ=255)
gTAACGATCGACTCCGCTTTCCCCGCTTTGCCGCCCTGCTTTGAGCAAACCAAACCATGCCCCCATCGc < 1:2381270/69‑1 (MQ=255)
gTAACGATCGACTCCGCTTTCCCCGCTTTGCCGCCCTGCTTTGAGCAAACCAAACCATGCCCCCATCGc > 1:2893652/1‑69 (MQ=255)
gATCGACTCCGCTTTCCCCGCTTTGCCTTCCTGCTTTCAGTAAACCAAACCATGCCCCCATCGCAATa < 1:392223/68‑1 (MQ=255)
gATCGACTCCGCTTTCCCCGCTTTGCCTTCCTGCTTTCAGCAAACCAAACCATGCCCCCATCGCAATa < 1:1073414/68‑1 (MQ=255)
gATCGACTCCGCTTTCCCCGCTTTGCCTTCCTGCTTTCAGCAAACCAAACCATGCCCCCATCGCAATa < 1:2805462/68‑1 (MQ=255)
gATCGACTCCGCTTTCCCCGCTTTGCCTTCCTGCTTTCAGCAAACCAAACCATGCCCCCATCGCAATa < 1:2907401/68‑1 (MQ=255)
gATCGACTCCGCTTTCCCCGCTTTGCCTTCCTGCTTTCAGCAAACCAAACCATGCCCCCATCGCAATa < 1:3157857/68‑1 (MQ=255)
gATCGACTCCGCTTTCCCCGCTTTGCCTTCCTGCTTTCAGCAAACCAAACCATGCCCCCATCGCAATa < 1:3161975/68‑1 (MQ=255)
gATCGACTCCGCTTTCCCCGCTTTGCCTTCCTGCTTTCAGCAAACCAAACCATGCCCCCATCGCAATa < 1:3221807/68‑1 (MQ=255)
gATCGACTCCGCTTTCCCCGCTTTGCCTTCCTGCTTTCAGCAAACCAAACCATGCCCCCATCGCAATa < 1:1993022/68‑1 (MQ=255)
gATCGACTCCGCTTTCCCCGCTTTGCCTTCCTGCTTTCAGCAAACCAAACCATGCCCCCATCGCAATa < 1:1823538/68‑1 (MQ=255)
gATCGACTCCGCTTTCCCCGCTTTGCCTTCCTGCTTTCAGCAAACCAAACCATGCCCCCATCGCAATa < 1:548890/68‑1 (MQ=255)
gATCGACTCCGCTTTCCCCGCTTTGCCTTCCTGCTTTCAGCAAACCAAACCATGCCCCCATCGCAATa < 1:562424/68‑1 (MQ=255)
gATCGACTCCGCTTTCCCCGCTTTGCCTTCCTGCTTTCAGCAAACCAAACCATGCCCCCATCGCAATa < 1:78993/68‑1 (MQ=255)
gATCGACTCCGCTTTCCCCGCTTTGCCTTCCTGCTTTCAGCAAACCAAACCATGCCCCCATCGCAATa < 1:1255186/68‑1 (MQ=255)
gATCGACTCCGCTTTCCCCGCTTTGCCTTCCTGCTTTCACCAAACCAAACCATGCCCCCATCGCAATa < 1:2108215/68‑1 (MQ=255)
gATCGACTCCGCTTTCCCCGCTTTCCCTTCCTGCTTTCAGCAAACCAAACCATGCCCCCATCGCAATa < 1:3375850/68‑1 (MQ=255)
aTCGACTCCGCTTTCCCCGCTTTGCCTTCCTGCTTTCAGCAAACCAAACCATGCCCCCATCGCAATa < 1:226383/67‑1 (MQ=255)
aTCGACTCCGCTTTCCCCGCTTTGCCTTCCTGCGTTCAGCAAACCAAACCATGCCCCCATCGCAATa < 1:2254521/67‑1 (MQ=255)
cTCCGCTTTCCCCGCTTTGCCGCCCTGCTTTGAGCAAACCAAACCATGCCCCCATCGCAATAGTTTCGt < 1:385688/69‑1 (MQ=255)
cTCCGCTTTCCCCGCTTTGCCGCCCTGCTTTGAGCAAACCAAACCATGCCCCCATCGCAATAGTTTCGt < 1:3186431/69‑1 (MQ=255)
cTCCGCTTTCCCCGCTTTGCCGCCCTGCTTTGAGCAAACCAAACCATGCCCCCATCGCAATAGTTTCGt < 1:400363/69‑1 (MQ=255)
cTCCGCTTTCCCCGCTTTGCCGCCCTGCTTTGAGCAAACCAAACCATGCCCCCATCGCAATAGTTTCGt < 1:996935/69‑1 (MQ=255)
cTCCGCTTTCCCCGCTTTGCCGCCCTGCTTTGAGCAAACCAAACCATGCCCCCATCGCAATAGTTTCGt < 1:2836950/69‑1 (MQ=255)
cTCCGCTTTCCCCGCTTTGCCGCCCTGCTTTGAGCAAACCAAACCATGCCCCCATCGCAATAGTTTCGt < 1:1161603/69‑1 (MQ=255)
cTCCGCTTTCCCCGCTTTGCCGCCCTGCTTTGAGCAAACCAAACCATGCCCCCATCGCAATAGTTTCGt < 1:2639506/69‑1 (MQ=255)
cTCCGCTTTCCCCGCTTTGCCGCCCTGCTTTGAGCAAACCAAACCATGCCCCCATCGCAATAGTTTCGt < 1:2268787/69‑1 (MQ=255)
cTCCGCTTTACCCGCTTTGCCGCCCTGCTTTGAGCAAACCAAACCATGCCCCCATCGCAATAGTTTCGt < 1:963835/69‑1 (MQ=255)
cTCCGCTTACCCCGCTTTGCCGCCCTGCTTTGAGCAAACCAAACCATGCCCCCATCGCAATAGTTTCGt < 1:2183461/69‑1 (MQ=255)
ttCCCCGCTTTGCCTTCCTGCTTTCAGCAAACCAAACCATGCCCCCATCGCAATAGTTTCGTTATAGCa < 1:1068112/69‑1 (MQ=255)
ttCCCCGCTTTGCCTTCCTGCTTTCAGCAAACCAAACCATGCCCCCATCGCAATAGTTTCGTTATAGCa < 1:979939/69‑1 (MQ=255)
ttCCCCGCTTTGCCTTCCTGCTTTCAGCAAACCAAACCATGCCCCCATCGCAATAGTTTCGTTATAGCa < 1:908169/69‑1 (MQ=255)
ttCCCCGCTTTGCCTTCCTGCTTTCAGCAAACCAAACCATGCCCCCATCGCAATAGTTTCGTTATAGCa < 1:1981830/69‑1 (MQ=255)
ttCCCCGCTTTGCCTTCCTGCTTTCAGCAAACCAAACCATGCCCCCATCGCAATAGTTTCGTTATAGCa < 1:2300000/69‑1 (MQ=255)
ccGCTTTGCCGCCCTGCTTTGAGCAAACCAAACCATGCCCCCATCGCAATAGTTTCGTTATAGCAAAc < 1:1786162/68‑1 (MQ=255)
ccGCTTTGCCGCCCTGCTTTGAGCAAACCAAACCATGCCCCCATCGCAATAGTTTCGTTATAGCAAAc < 1:3385371/68‑1 (MQ=255)
ttGCCGCCCTGCTTTGAGCAAACCAAACCATGCCCCCATCGCAATAGTTTCGTTATAGCAAACCACGGc < 1:1588512/69‑1 (MQ=255)
ttGCCGCCCTGCTTTGAGCAAACCAAACCATGCCCCCATCGCAATAGTTTCGTTATAGCAAACCACGGc < 1:463722/69‑1 (MQ=255)
gccCTGCTTTGAGCAAACCAAACCATGCCCCCATCGCAATAGTTTCGTTATAGCAAACCACGGCACTg < 1:3414051/68‑1 (MQ=255)
ccTGCTTTCAGCAAACCACACCATGCCCCCATCGCAATAGTTTCGTTATAGCAAACCACGGCGCTGAt > 1:1893846/1‑68 (MQ=255)
ccTGCTTTCAGCAAACCAAACCATGCCCCCATCGCAATAGTTTCGTTATAGCAAAcc > 1:1242187/1‑57 (MQ=255)
ccTGCTTTCAGCAAACCAAACCATGCCCCCATCGCAATAGTTTCGTTATAGCAAACCACGGCGCTGAt > 1:3362006/1‑68 (MQ=255)
ccTGCTTTCAGCAAACCAAACCATGCCCCCATCGCAATAGTTTCGTTATAGCAAACCACGGCGCTGATg > 1:2611376/1‑69 (MQ=255)
ccTGCTTTCAGCAAACCAAACCATGCCCCCATCGCAATAGTTTCGTTATAGCAAACCACGGCGCTGATg > 1:351723/1‑69 (MQ=255)
ccTGCTTTCAGCAAACCAAACCATGCCCCCATCGCAATAGTTTCGTTATAGCAAACCACGGCGCTGATg > 1:1807203/1‑69 (MQ=255)
ccTGCTTTCAGCAAACCAAACCATGCCCCCATCGCAATAGTTTCGTTATAGCAAACCACGGCGCTGATg > 1:2423142/1‑69 (MQ=255)
ccTGCTTTCAGCAAACCAAACCATGCCCCCATCGCAATAGTTTCGTTATAGCAAACCACGGCGCTGATg > 1:930032/1‑69 (MQ=255)
ccTGCTTTCAGCAAACCAAACCATGCCCCCATCGCAATAGTTTCGTTATAGCAAACCACGGCGCTGATg > 1:2658988/1‑69 (MQ=255)
ccTGCTTTCAGCAAACCAAACCATGCCCCCATCGCAATAGTTTCGTTATAGCAAACCACGGCGCTGATg > 1:729160/1‑69 (MQ=255)
ccTGCTTTCAGCAAACCAAACCATGCCCCCATCGCAATAGTTTCGTTATAGCAAACCACGGCGCTGATg > 1:2609861/1‑69 (MQ=255)
ccTGCTTTCAGCAAACCAAACCATGCCCCCATCGCAATAGTTTCGTTATAGCAAACCACGGCGCTGATg > 1:1644499/1‑69 (MQ=255)
ccTGCTTTCAGCAAACCAAACCATGCCCCCATCGCAATAGTTTCGTTATAGCAAACCACGGCGCTGATg > 1:1365884/1‑69 (MQ=255)
ccTGCTTTCAGCAAACCAAACCATGCCCCCATCGCAATAGTTTCGTTATAGCAAACCACGGCGCTGATg > 1:2871137/1‑69 (MQ=255)
ccTGCTTTCAGCAAACCAAACCATGCCCCCATCGCAATAGTTTAGTTATAGCAAACCACGGCGCTGATg > 1:3093334/1‑69 (MQ=255)
acTGCTTTGAGCAAACCAAACCATGCCCCCATCGCAAt < 1:1632187/37‑1 (MQ=255)
tGCTTTGAGCAAACCAAACCATGCCCCCATCGCAATAGTTTCGTTATAGCAAAc < 1:1246849/54‑1 (MQ=255)
gCTTTGAGCAAACCAAACCATGCCCCCATCGCAAt < 1:3085584/35‑1 (MQ=255)
gCTTTGAGCAAACCAAACCATGCCCCCATCGCAAt < 1:2811007/35‑1 (MQ=255)
gCTTTGAGCAAACCAAACCATGCCCCCATCGCAAt < 1:724183/35‑1 (MQ=255)
gCTTTGAGCAAACCAAACCATGCCCCCATCGCAAt < 1:744525/35‑1 (MQ=255)
gCTTTGAGCAAACCAAACCATGCCCCCATCGCAAt < 1:2407023/35‑1 (MQ=255)
gCTTTGAGCAAACCAAACCATGCCCCCATCGCAAt < 1:1665346/35‑1 (MQ=255)
gCTTTGAGCAAACCAAACCATGCCCCCATCGCAAt < 1:2419979/35‑1 (MQ=255)
|
GCCGCCAGCGAAACCTGTTGCTCAAAGTAACGATCGACTCCGCTTTCCCCGCTTTGCCGCCCTGCTTTGAGCAAACCAAACCATGCCCCCATCGCAATAGTTTCGTTATAGCAAACCACGGCACTGATG > minE/1026015‑1026143
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
