Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F1 I0 R1 4577 57.4 3436498 77.7% 2670158 63.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,037,042 C→T 39.5% A70A (GCC→GCT) ‡ rsxD → predicted inner membrane oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,037,0420CT39.5% 49.7 / 67.6 101A70A (GCC→GCT) ‡rsxDpredicted inner membrane oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (17/44);  new base T (11/29);  total (28/73)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.00e-01

Only 100 of 101 total aligned reads displayed.

GGCATCGGTTAGTGCTCTGTTAGCCGAAGCTCTCGTACTCAAACTACGCAAGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGCTGGCGGTAAG  >  minE/1036979‑1037095
                                                               |                                                     
ggCATCGGTTAGTGCTCTGTTAGCCGAAGCTCTCGTACTCAAACTACGCAAGCAGTCGGTAGCCGCAAc                                                  >  1:65755/1‑69 (MQ=255)
     cGGTCAGTGCTCTTCTGGCCGAAGCTCTCGTCCTCAAACTACGCAAGCAGTCGGTAGCTGCAACGTTGa                                             <  1:3010080/69‑1 (MQ=255)
     cGGTCAGTGCTCTTCTGGCCGAAGCTCTCGTCCTCAAACTACGCAAGCAGTCGGTAGCTGCAACGTTGa                                             <  1:1350729/69‑1 (MQ=255)
     cGGTCAGTGCTCTTCTGGCCGAAGCTCTCGTCCTCAAACTACGCAAGCAGTCGGTAGCTGCAACGTTGa                                             <  1:571809/69‑1 (MQ=255)
     cGGTCAGTGCTCTTCTGGCCGAAGCTCTCGTCCTCAAACTACGCAAGCAGTCGGTAGCTGCAACGTTGa                                             <  1:2772357/69‑1 (MQ=255)
      ggTCAGTGCTCTTCTGGCCGAAGCTCTCGTCCTCAAACTACGCAAGCAGTCGGTAGCTGCAACGTTGa                                             <  1:3377708/68‑1 (MQ=255)
        ttAGTGCTCTGTTAGCCGAAGCTCTCGTACTCAAACTACGCAAGCAGTCGGTAGCCGCAACGTTGaaa                                           <  1:659864/68‑1 (MQ=255)
        ttAGTGCTATGTTAGCCGAAGCTCTCGTACTCAAACTACGCAAGCAGTCGGTAGCCGCAACGTTGaaa                                           <  1:2297133/68‑1 (MQ=255)
                     aGCCGAAGCTCTCGTACTCAAACTACGCAAGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGc                                <  1:1808305/66‑1 (MQ=255)
                     aGCCGAAGCTCTCGTACTCAAACTACGCAAGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCAtt                             <  1:2877315/69‑1 (MQ=255)
                     aGCCGAAGCTCTCGTACTCAAACTACGCAAGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCAtt                             <  1:3004360/69‑1 (MQ=255)
                        cGAAGCTCTCGTACTCAAACTACGCAAGCAGTCTGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCt                          <  1:221015/69‑1 (MQ=255)
                        cGAAGCTCTCGTACTCAAACTACGCAAGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCt                          <  1:15712/69‑1 (MQ=255)
                        cGAAGCTCTCGTACTCAAACTACGCAAGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCt                          <  1:359722/69‑1 (MQ=255)
                        cGAAGCTCTCGTACTCAAACTACGCAAGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCt                          <  1:3027985/69‑1 (MQ=255)
                        cGAAGCTCTCGTACTCAAACTACGCAAGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCt                          <  1:1709890/69‑1 (MQ=255)
                        cGAAGCTCTCGTACTCAAACTACGCAAGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCt                          <  1:728430/69‑1 (MQ=255)
                         gAAGCTCTCGTCCTCAAACTACGCAAGCAGTCGGTAGCTGCaa                                                   >  1:1840134/1‑43 (MQ=38)
                         gAAGCTCTCGTCCTCAAACTACGCAAGCAGTCGGTAGCTGCAACGTTGAAAGATAACTCAGCATTGCt                          >  1:1397988/1‑68 (MQ=255)
                         gAAGCTCTCGTCCTCAAACTACGCAAGCAGTCGGTAGCTGCAACGTTGAAAGATAACTCAGCATTGCt                          >  1:3409183/1‑68 (MQ=255)
                         gAAGCTCTCGTCCTCAAACTACGCAAGCAGTCGGTAGCTGCAACGTTGAAAGATAACTCAGCATTGCt                          >  1:147662/1‑68 (MQ=255)
                         gAAGCTCTCGTCCTCAAACTACGCAAGCAGTCGGTAGCTGCAACGTTGAAAGATAACTCAGCATTGCt                          >  1:3139442/1‑68 (MQ=255)
                         gAAGCTCTCGTCCTCAAACTACGCAAGCAGTCGGTAGCTGCAACGTTGAAAGATAACTCAGCATTGCt                          >  1:813178/1‑68 (MQ=255)
                         gAAGCTCTCGTCCTCAAACTACGCAAGCAGTCGGTAGCTGCAACGTTGAAAGATAACTCAGCATTGCt                          >  1:2006612/1‑68 (MQ=255)
                         gAAGCTCTCGTCCTCAAACTACGCAAGCAGTCGGTAGCTGCAACGTTGAAAGATAACTCAGCATTGCt                          >  1:2331652/1‑68 (MQ=255)
                         gAAGCTCTCGTCCTCAAACTACGCAAGCAGTCGGTAGCTGCAACGTTGAAAGATAACTCAGCATTGCt                          >  1:1251899/1‑68 (MQ=255)
                         gAAGCTCTCGTCCTCAAACTACGCAAGCAGTCGGTAGCTGCAACGTTGAAAGATAACTCAGCATTGCt                          >  1:1651220/1‑68 (MQ=255)
                         gAAGCTCTCGTCCTCAAACTACGCAAGCAGTCGGTAGCTGCAACGTTGAAAGATAACTCAGCATTGCt                          >  1:1469886/1‑68 (MQ=255)
                         gAAGCTCTCGTACTCAAACTACGCAAGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCt                          <  1:1886223/68‑1 (MQ=255)
                         gAAGCTCTCGTACTCAAACTACGCAAGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCt                          >  1:1600081/1‑68 (MQ=255)
                         gAAGCTCTCGTACTCAAACTACGCAAGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCt                          >  1:2083345/1‑68 (MQ=255)
                         gAAGCTCTCGTACTCAAACTACGCAAGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCt                          >  1:3343237/1‑68 (MQ=255)
                         gAAGCTCTCGTACTCAAACTACGCAAGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCt                          >  1:1533595/1‑68 (MQ=255)
                                  gTACTCAAACTACGCAAGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCt                  <  1:1547181/67‑1 (MQ=255)
                                  gTACTCAAACTACGCAAGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCt                  <  1:1985353/67‑1 (MQ=255)
                                  gTACTCAAACTACGCAAGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCt                  <  1:2294686/67‑1 (MQ=255)
                                  gTACTCAAACTACGCAAGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCt                  <  1:2972689/67‑1 (MQ=255)
                                     cTCAAACTACGCAATCAGTCGGTAGTCGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCtt                 <  1:434884/65‑1 (MQ=255)
                                     cTCAAACTACGCAAGCAGTCGGTAGTCGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCtt                 <  1:3159904/65‑1 (MQ=255)
                                     cTCAAACTACGCAAGCAGTCGGTAGTCGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCtt                 >  1:2826912/1‑65 (MQ=255)
                                     cTCAAACTACGCAAGCAGTCGGTAGTCGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCtt                 <  1:1590212/65‑1 (MQ=255)
                                     cTCAAACTACGCAAGCAGTCGGTAGTCGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCtt                 >  1:2933331/1‑65 (MQ=255)
                                     cTCAAACTACGCAAGCAGTCGGTAGTCGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCtt                 >  1:2704759/1‑65 (MQ=255)
                                     cTCAAACTACGCAAGCAGTCGGTAGTCGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCtt                 <  1:2638276/65‑1 (MQ=255)
                                     cTCAAACTACGCAAGCAGTCGGTAGTCGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCtt                 >  1:2576733/1‑65 (MQ=255)
                                     cTCAAACTACGCAAGCAGTCGGTAGTCGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCtt                 >  1:2440288/1‑65 (MQ=255)
                                     cTCAAACTACGCAAGCAGTCGGTAGTCGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCtt                 >  1:364916/1‑65 (MQ=255)
                                      tCAAACTACGCAAGCAGTCGGTAGTCGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCtt                 <  1:622340/64‑1 (MQ=255)
                                       cAAACTACGCAAGCAGTCGGTAGCTGCAACGTTGAAATATAACTCAGCATTGCTGACAGGCTTATTGCt           <  1:1602471/69‑1 (MQ=255)
                                       cAAACTACGCAAGCAGTCGGTAGCTGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGCt           <  1:3017599/69‑1 (MQ=255)
                                       cAAACTACGCAAGCAGTCGGTAGCTGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGCt           <  1:540832/69‑1 (MQ=255)
                                       cAAACTACGCAAGCAGTCGGTAGCTGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGCt           <  1:48683/69‑1 (MQ=255)
                                       cAAACTACGCAAGCAGTCGGTAGCTGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGCt           <  1:563403/69‑1 (MQ=255)
                                       cAAACTACGCAAGCAGTCGGTAGCTGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGCt           <  1:2661576/69‑1 (MQ=255)
                                       cAAACTACGCAAGCAGTCGGTAGCTGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGCt           <  1:415798/69‑1 (MQ=255)
                                       cAAACTACGCAAGCAGTCGGTAGCTGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGCt           <  1:2878883/69‑1 (MQ=255)
                                       cAAACTACGCAAGCAGTCGGTAGCTGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGCt           <  1:3163606/69‑1 (MQ=255)
                                       cAAACTACGCAAGCAGTCGGTAGCTGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGCt           <  1:932738/69‑1 (MQ=255)
                                       cAAACTACGCAAGCAGTCGGTAGCTGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGCt           <  1:1184421/69‑1 (MQ=255)
                                       cAAACTACGCAAGCAGTCGGTAGCTGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGCt           <  1:2070379/69‑1 (MQ=255)
                                       cAAACTACGCAAGCAGTCGGTAGCTGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGCt           <  1:2726499/69‑1 (MQ=255)
                                       cAAACTACGCAAGCAGTCGGTAGCTGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGCt           <  1:119464/69‑1 (MQ=255)
                                       cAAACTACGCAAGCAGTCGGTAGCTGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGCt           <  1:1913132/69‑1 (MQ=255)
                                       cAAACTACGCAAGCAGTCGGTAGCTGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGCt           <  1:1465039/69‑1 (MQ=255)
                                       cAAACTACGCAAGCAGTCGGTAGCTGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGCt           <  1:2823814/69‑1 (MQ=255)
                                       cAAACTACGCAAGCAGTCGGTAGCTGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGCt           <  1:2500688/69‑1 (MQ=255)
                                       cAAACTACGCAAGCAGTCGGTAGCTGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGCt           <  1:2148084/69‑1 (MQ=255)
                                       cAAACTACGCAAGCAGTCGGTAGCTGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGCt           <  1:2286065/69‑1 (MQ=255)
                                       cAAACTACGCAAGCAGTCGGCAGCTGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGCt           <  1:2141211/69‑1 (MQ=255)
                                            tACGCAAGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGc            <  1:2032675/63‑1 (MQ=255)
                                            tACGCAAGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGc            <  1:3252139/63‑1 (MQ=255)
                                            tACGCAAGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGc            <  1:2246758/63‑1 (MQ=255)
                                            tACGCAAGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGc            <  1:638888/63‑1 (MQ=255)
                                            tACGCAAGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGc            <  1:1271786/63‑1 (MQ=255)
                                             aCGCAAGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGc            <  1:2157635/62‑1 (MQ=255)
                                              cGCAAGCAGTCGGGAGCTGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGCt           <  1:1702929/62‑1 (MQ=255)
                                               gCAAGCAGTCGGTAGCTGCAACGTTGAAAGATAACt                                    <  1:402539/36‑1 (MQ=255)
                                                cAAGCAGTCGTTAGCCGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGCTGGCGGTAAg  <  1:3047546/69‑1 (MQ=255)
                                                cAAGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGCTGGCGGTAAg  <  1:1668582/69‑1 (MQ=255)
                                                cAAGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGCTGGCGGTAAg  <  1:1676279/69‑1 (MQ=255)
                                                cAAGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGCTGGCGGTAAg  <  1:1734249/69‑1 (MQ=255)
                                                cAAGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGCTGGCGGTAAg  <  1:1554610/69‑1 (MQ=255)
                                                cAAGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGCTGGCGGTAAg  <  1:85543/69‑1 (MQ=255)
                                                cAAGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGCTGGCGGTAAg  <  1:2085565/69‑1 (MQ=255)
                                                cAAGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGCTGGCGGTAAg  <  1:2075246/69‑1 (MQ=255)
                                                cAAGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGCTGGCGGTAAg  <  1:3216739/69‑1 (MQ=255)
                                                cAAGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGCTGGCGGTAAg  <  1:2418930/69‑1 (MQ=255)
                                                cAAGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGCTGGCGGTAAg  <  1:2640606/69‑1 (MQ=255)
                                                cAAGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGCTGGCGGTAAg  <  1:1144082/69‑1 (MQ=255)
                                                cAAGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGCTGGCGGTAAg  <  1:2995014/69‑1 (MQ=255)
                                                cAAGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGCTGGCGGTAAg  <  1:2724518/69‑1 (MQ=255)
                                                cAAGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGCTGGCGGTAAg  <  1:2932133/69‑1 (MQ=255)
                                                 aaGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGCTGGCGGTAAg  <  1:3220427/68‑1 (MQ=255)
                                                  aGCAGTCGGTAGCCGTAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGCTGGCGGTaa   >  1:3189989/1‑66 (MQ=255)
                                                  aGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGCTGGCGGTaa   >  1:2057668/1‑66 (MQ=255)
                                                  aGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGCTGGCGGTaa   >  1:457108/1‑66 (MQ=255)
                                                  aGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGCTGGCGGTaa   >  1:63505/1‑66 (MQ=255)
                                                  aGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGCTGGCGGTaa   >  1:2916252/1‑66 (MQ=255)
                                                  aGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGCTGGAGGTaa   >  1:724407/1‑66 (MQ=255)
                                                        cGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGc            <  1:3333831/51‑1 (MQ=255)
                                                               |                                                     
GGCATCGGTTAGTGCTCTGTTAGCCGAAGCTCTCGTACTCAAACTACGCAAGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGCTGGCGGTAAG  >  minE/1036979‑1037095

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A