Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,053,815 |
A→C |
31.1% |
L6F (TTA→TTC) ‡ |
ydhM → |
predicted DNA‑binding transcriptional regulator |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,053,815 | 0 | A | C | 31.1%
| 56.6
/ 40.1
| 61 | L6F (TTA→TTC) ‡ | ydhM | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base A (20/22); new base C (15/4); total (35/26) |
| Fisher's exact test for biased strand distribution p-value = 2.73e-02 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CTCCTGGCGACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCACTACTTTCGCTCTAAAGAAGCGTTTGGC > minE/1053750‑1053881
|
ctcCTGGCGACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACt > 1:1212965/1‑68 (MQ=255)
gACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCg < 1:1008473/68‑1 (MQ=255)
gACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCg < 1:2462003/68‑1 (MQ=255)
gACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCg < 1:2370844/68‑1 (MQ=255)
gACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCg < 1:3424511/68‑1 (MQ=255)
gACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCg < 1:1845240/68‑1 (MQ=255)
gACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCg < 1:1838884/68‑1 (MQ=255)
gACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCg < 1:163864/68‑1 (MQ=255)
gACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCg < 1:1582858/68‑1 (MQ=255)
gACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCg < 1:89007/68‑1 (MQ=255)
gggCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGa > 1:3402628/1‑69 (MQ=255)
ttGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAgg > 1:627333/1‑69 (MQ=255)
tGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAggg > 1:518159/1‑69 (MQ=255)
tGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAggg > 1:888620/1‑69 (MQ=255)
tGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAggg > 1:469612/1‑69 (MQ=255)
tGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAggg > 1:219108/1‑69 (MQ=255)
tGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAggg > 1:1103375/1‑69 (MQ=255)
ccTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGt < 1:2833049/68‑1 (MQ=255)
ccTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGt < 1:1311416/68‑1 (MQ=255)
ccTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGt < 1:2717614/68‑1 (MQ=255)
ccTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGt < 1:3320436/68‑1 (MQ=255)
ccTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGt < 1:256294/68‑1 (MQ=255)
ccTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGt < 1:2454001/68‑1 (MQ=255)
ccTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGt < 1:1874319/68‑1 (MQ=255)
ccTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGt < 1:1199054/68‑1 (MQ=255)
cTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAATGGt < 1:3407114/67‑1 (MQ=255)
cAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTc > 1:3419975/1‑69 (MQ=255)
cAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTc > 1:906531/1‑69 (MQ=255)
cAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTc > 1:48351/1‑69 (MQ=255)
cAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTc > 1:610911/1‑69 (MQ=255)
cAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTc > 1:580387/1‑69 (MQ=255)
cAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTc > 1:1771595/1‑69 (MQ=255)
cGTGGATTCACCGGGATGGGGCTAAGCGAACTCCTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTAt > 1:1267001/1‑69 (MQ=255)
cGTGGATTCACCGGGATGGGGCTAAGCGAACTCCTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTAt > 1:1296514/1‑69 (MQ=255)
cGTGGATTCACCGGGATGGGGCTAAGCGAACTCCTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTAt > 1:534519/1‑69 (MQ=255)
cGTGGATTCACCGGGATGGGGCTAAGCGAACTCCTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTAt > 1:12696/1‑69 (MQ=255)
cGTGGATTCACCGGGATGGGGCTAAGCGAACTCCTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTAt > 1:1955296/1‑69 (MQ=255)
cGTGGATTCACCGGGATGGGGCTAAGCGAACTCCTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTAt > 1:3431263/1‑69 (MQ=255)
cGTGGATTCACCGGGATGGGGCTAAGCGAACTCCTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTAt > 1:2345846/1‑69 (MQ=255)
cGTGGATTCACCGGGATGGGGCTAAGCGAACTCCTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTAt > 1:2619517/1‑69 (MQ=255)
cGTGGATTCACCGGGATGGGGCTAAGCGAACTCCTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTAt > 1:3238095/1‑69 (MQ=255)
cGTGGATTCACCGGGATGGGGCTAAGCGAACTCCTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTAt > 1:901675/1‑69 (MQ=255)
cGTGGATTCACCGGGATGGGGCTAAGCGAACTCCTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTAt > 1:1011245/1‑69 (MQ=255)
cGTGGATTCACCGGGATGGGGCTAAGCGAACTCCTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTAt > 1:3077225/1‑69 (MQ=255)
cGTGGATTCACCGGGATGGGGCTAAGCGAACTCCTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTAt > 1:3042078/1‑69 (MQ=255)
cGTGGATTCACCGGGATGGGGCTAAGCGAACTCCTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTAt > 1:2900470/1‑69 (MQ=255)
cGTGGATTCACCGGGATGGGGCTAAGCGAACTCCTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTAt > 1:2835458/1‑69 (MQ=255)
tCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCactact > 1:3384086/1‑69 (MQ=255)
tCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCactact > 1:2631105/1‑69 (MQ=255)
tCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCactact > 1:672780/1‑69 (MQ=255)
tCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCactact > 1:3165146/1‑69 (MQ=255)
tCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCactact > 1:3008817/1‑69 (MQ=255)
tCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCactact > 1:2946531/1‑69 (MQ=255)
cGAACTCCTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCACTACTTTCGTTCTAAAGAAGCg < 1:2867968/67‑1 (MQ=255)
cGAACTCCTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCACTACTTTCGTTCTAAAGAAGCg < 1:1391079/67‑1 (MQ=255)
cGAACTCCTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCACTACTTTCGTTCTAAAGAAGCg < 1:319838/67‑1 (MQ=255)
cGAACTCCTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCACTACTTTCGTTCTAAAGAAGCg < 1:3170225/67‑1 (MQ=255)
aTTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCACTACTTTCGGTCTAAAGAAGCGTTTgg < 1:1623495/69‑1 (MQ=255)
ttACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCACTACTTTCGCTCTAAAGAAGCGTTTGGc < 1:2421808/69‑1 (MQ=255)
ttACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCACTACTTTCGCTCTAAAGAAGCGTTTGGc < 1:2715279/69‑1 (MQ=255)
ttACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCACTACTTTCGCTCTAAAGAAGCGTTTGGc < 1:1194615/69‑1 (MQ=255)
|
CTCCTGGCGACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCACTACTTTCGCTCTAAAGAAGCGTTTGGC > minE/1053750‑1053881
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
