Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,076,180 |
C→T |
29.0% |
I453I (ATC→ATT) |
ydhS → |
conserved hypothetical protein with FAD/NAD(P)‑binding domain |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,076,180 | 0 | C | T | 29.0%
| 81.9
/ 32.8
| 79 | I453I (ATC→ATT) | ydhS | conserved hypothetical protein with FAD/NAD(P)‑binding domain |
| Reads supporting (aligned to +/- strand): ref base C (23/33); new base T (5/18); total (28/51) |
| Fisher's exact test for biased strand distribution p-value = 1.26e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.39e-01 |
GACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGA > minE/1076112‑1076245
|
gACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATc < 1:206815/69‑1 (MQ=255)
gACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATc < 1:878741/69‑1 (MQ=255)
gACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATc < 1:2513551/69‑1 (MQ=255)
gACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATc < 1:740025/69‑1 (MQ=255)
gACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATc < 1:161529/69‑1 (MQ=255)
gACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATc < 1:2688827/69‑1 (MQ=255)
gACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATc < 1:15467/69‑1 (MQ=255)
tGACGTTTTTATTGATGCCCGTGGGCAGCGTCCGCTTAAAGTGAAAGATATTCCTTTCCCTGGACTAc > 1:2224657/1‑68 (MQ=255)
tGACGTTTTTATTGATGCCCGTGGGCAGCGTCCGCTTAAAGTGAAAGATATTCCTTTCCCTGGACTAc > 1:1641089/1‑68 (MQ=255)
tGACGTTTTTATTGATGCCCGTGGGCAGCGTCCGCTTAAAGTGAAAGATATTCCTTTCCCTGGACTAc > 1:2598357/1‑68 (MQ=255)
tGACGTTTTTATTGATGCCCGTGGGCAGCGTCCGCTTAAAGTGAAAGATATTCCTTTCCCTGGACTAc > 1:1224745/1‑68 (MQ=255)
tGACGTTTTTATTGATGCCCGTGGGCAGCGTCCGCTTAAAGTGAAAGATATTCCTTTCCCTGGACTAc > 1:1561542/1‑68 (MQ=255)
tGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATc < 1:1001054/52‑1 (MQ=255)
tGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATc < 1:1197061/52‑1 (MQ=255)
tGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTAc > 1:2737625/1‑68 (MQ=255)
tGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTAc > 1:2779412/1‑68 (MQ=255)
tGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTAc > 1:2933413/1‑68 (MQ=255)
tGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTAc > 1:858842/1‑68 (MQ=255)
tGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTAc > 1:911511/1‑68 (MQ=255)
tttttATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGc < 1:2736850/69‑1 (MQ=255)
tttttATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGc < 1:968432/69‑1 (MQ=255)
tttttATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGc < 1:1640326/69‑1 (MQ=255)
ttttATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCa < 1:472269/69‑1 (MQ=255)
ttttATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCa < 1:1061679/69‑1 (MQ=255)
ttttATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCa < 1:2154327/69‑1 (MQ=255)
tttATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGc < 1:1891152/67‑1 (MQ=255)
tttATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGc < 1:1203737/67‑1 (MQ=255)
tttATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCa > 1:1235240/1‑68 (MQ=255)
tttATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCa > 1:2784898/1‑68 (MQ=255)
tttATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCa > 1:1060416/1‑68 (MQ=255)
gATGCCGGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACa > 1:3266705/1‑68 (MQ=255)
gATGCCCTCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACa > 1:787229/1‑68 (MQ=255)
gATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACa > 1:1078686/1‑68 (MQ=255)
gATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACa > 1:3039718/1‑68 (MQ=255)
gATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACa > 1:2170766/1‑68 (MQ=255)
gATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACa > 1:33372/1‑68 (MQ=255)
gATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACa > 1:3350062/1‑68 (MQ=255)
gATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACa > 1:2437516/1‑68 (MQ=255)
gATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCCAGCAATTACa > 1:1600531/1‑68 (MQ=255)
cgcgGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAAc < 1:704549/68‑1 (MQ=255)
cgcgGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAAc < 1:3102356/68‑1 (MQ=255)
aCGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGa < 1:1963861/69‑1 (MQ=255)
aCGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGa < 1:2285654/69‑1 (MQ=255)
aCGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGa < 1:1813946/69‑1 (MQ=255)
tCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAAt < 1:30388/69‑1 (MQ=255)
ccGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATc < 1:3248732/69‑1 (MQ=255)
ccGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATc < 1:2557028/69‑1 (MQ=255)
ccGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATc < 1:2797619/69‑1 (MQ=255)
ccGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATc < 1:296943/69‑1 (MQ=255)
ccGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATc < 1:1737017/69‑1 (MQ=255)
ccGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATc < 1:2182327/69‑1 (MQ=255)
ccGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATc < 1:2098149/69‑1 (MQ=255)
ccGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATc < 1:444412/69‑1 (MQ=255)
cTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTAAAGAAAACAGGGGATGAAATc < 1:2947486/66‑1 (MQ=255)
ttaaagttaaagATATCCCTTTCCCTGGGCTACGCGAGc < 1:2146518/39‑1 (MQ=255)
ttAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGc < 1:1256279/39‑1 (MQ=255)
ttAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGc < 1:2209602/39‑1 (MQ=255)
ttAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGc < 1:3396276/39‑1 (MQ=255)
ttAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGc < 1:645017/39‑1 (MQ=255)
ttAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGc < 1:1777116/39‑1 (MQ=255)
ttAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGc < 1:556286/39‑1 (MQ=255)
tAAAGTGAAAGATATCCCTTTCCCTGGGGTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGa > 1:3371641/1‑69 (MQ=255)
tAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGc < 1:3305092/38‑1 (MQ=255)
tAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGa > 1:2720623/1‑69 (MQ=255)
tAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGa > 1:1434324/1‑69 (MQ=255)
tAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGa > 1:2578371/1‑69 (MQ=255)
tAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGa > 1:904231/1‑69 (MQ=255)
tAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGa > 1:1048341/1‑69 (MQ=255)
aGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATc < 1:2102108/61‑1 (MQ=255)
tatTCCTTTCCCTGGACTACGCGAACAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGa < 1:2837528/69‑1 (MQ=255)
tatTCCTTTCCCTGGACTACGCGAACAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGa < 1:850307/69‑1 (MQ=255)
tatTCCTTTCCCTGGACTACGCGAACAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGa < 1:891151/69‑1 (MQ=255)
tatTCCTTTCCCTGGACTACGCGAACAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGa < 1:603438/69‑1 (MQ=255)
tatTCCTTTCCCTGGACTACGCGAACAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGa < 1:1006432/69‑1 (MQ=255)
tatTCCTTTCCCTGGACTACGCGAACAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGa < 1:434242/69‑1 (MQ=255)
tatTCCTTTCCCTGGACTACGCGAACAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGa < 1:410742/69‑1 (MQ=255)
tatTCCTTTCCCTGGACTACGCGAACAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGa < 1:3435040/69‑1 (MQ=255)
tatTCCTTTCCCTGGACTACGCGAACAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGa < 1:3232017/69‑1 (MQ=255)
tatTCCTTTCCCTGGACTACGCGAACAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGa < 1:27734/69‑1 (MQ=255)
tatTCCTTTCCCTGGACTACGCGAACAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGa < 1:2705195/69‑1 (MQ=255)
tatTCCTTTCCCTGGACTACGCGAACAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGa < 1:2531267/69‑1 (MQ=255)
tatTCCTTTCCCTGGACTACGCGAACAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGa < 1:1677124/69‑1 (MQ=255)
tatTCCTTTCCCTGGACTACGCGAACAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGa < 1:2132796/69‑1 (MQ=255)
tatTCCTTTCCCTGGACTACGCGAACAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGa < 1:2120397/69‑1 (MQ=255)
tatTCCTTTCCCTGGACTACGCGAACAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGa < 1:1943505/69‑1 (MQ=255)
tatTCCTTTCCCTGGACTACGCGAACAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGa < 1:1936989/69‑1 (MQ=255)
tatTCCTTTCCCTGGACTACGCGAACAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGa < 1:1827687/69‑1 (MQ=255)
tatCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGggagga < 1:423809/48‑1 (MQ=255)
|
GACAACAGCTACTCGTTTGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGA > minE/1076112‑1076245
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
