Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,121,626 |
T→C |
58.0% |
M59V (ATG→GTG) |
btuE ← |
predicted glutathione peroxidase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,121,626 | 0 | T | C | 58.0%
| 21.9
/ 54.2
| 76 | M59V (ATG→GTG) | btuE | predicted glutathione peroxidase |
| Reads supporting (aligned to +/- strand): ref base T (16/16); new base C (23/21); total (39/37) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACA > minE/1121562‑1121692
|
ttAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATaaaa > 1:1028110/1‑69 (MQ=255)
ctctTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATTCCAGCACCATAAAACCtc > 1:2263581/1‑68 (MQ=255)
tCGCTACCCGGTTCTTGCTCCAGAAACTGGTTACACGGGAATCCCAGCACCACAAAACCacgatca < 1:798282/66‑2 (MQ=255)
tCGCTACCCGGTTCTTGCTCCAGAAACTGGTTACACGGGAATCCCAGCACCACAAAACCacgatca < 1:2278240/66‑2 (MQ=255)
tCGCTACCCGGTTCTTGCTCCAGAAACTGGTTACACGGGAATCCCAGCACCACAAAACCacgatca < 1:427827/66‑2 (MQ=255)
tCGCTACCCGGTTCTTGCTCCAGAAACTGGTTACACGGGAATCCCAGCACCACAAAACCacgatca < 1:2216503/66‑2 (MQ=255)
tCGCTACCCGGTTCTTGCTCCAGAAACTGGTTACACGGGAATCCCAGCACCACAAAACCacgatca < 1:2127998/66‑2 (MQ=255)
tCGCTACCCGGTTCTTGCTCCAGAAACTGGTTACACGGGAATCCCAGCACCACAAAACCacgatca < 1:806974/66‑2 (MQ=255)
tCGCTACCCGGTTCTTGCTCCAGAAACTGGTTACACGGGAATCCCAGCACCACAAAACCacgatca < 1:2536025/66‑2 (MQ=255)
tCGCTACCCGGTTCTTGCTCCAGAAACTGGTTACACGGGAATCCCAGCACCACAAAACCacgatca < 1:3174739/66‑2 (MQ=255)
tCGCTACCCGGTTCTTGCTCCAGAAACTGGTTACACGGGAATCCCAGCACCACAAAACCacgatca < 1:2941768/66‑2 (MQ=255)
tCGCTACCCGGTTCTTGCTCCAGAAACTGGTTACACGGGAATCCCAGCACCACAAAACCacgatca < 1:2571501/66‑2 (MQ=255)
tCGCTACCCGGTTCTTGCTCCAGAAACTGGTTACACGGGAATCCCAGCACCACAAAACCacgatca < 1:2855923/66‑2 (MQ=255)
tCGCTACCCGGTTCTTGCTCCAGAAACTGGTTACACGGGAATCCCAGCACCACAAAACCacgatca < 1:1824498/66‑2 (MQ=255)
tCGCTACCCGGTTCTTGCTCCAGAAACTGGTTACACGCGAATCCCAGCACCACAAAACCacgatca < 1:1839766/66‑2 (MQ=25)
tcTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCa > 1:2783808/2‑69 (MQ=255)
gCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCa > 1:2333210/1‑69 (MQ=255)
gCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCa > 1:3428475/1‑69 (MQ=255)
gCTGCCCGGTTCTTGTTCCAGAAACTGGTTCCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCa > 1:631572/1‑69 (MQ=255)
ccAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATtc > 1:2003058/1‑68 (MQ=255)
ccAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATtc > 1:899645/1‑68 (MQ=255)
ccAGAAACTGGTTACACGGGAATCCCAGCACCACAAAACCACGATCAGCCCAGGCTTTCTGAATATtc < 1:929009/68‑1 (MQ=255)
ccAGAAACTGGTTACACGGGAATCCCAGCACCACAAAACCACGATCAGCCCAGGCTTTCTGAATATtc < 1:2272876/68‑1 (MQ=255)
ccAGAAACTGGTTACACGGGAATCCCAGCACCACAAAACCACGATCAGCCCAGGCTTTCTGAATATtc < 1:541073/68‑1 (MQ=255)
ccAGAAACTGGTTACACGGGAATCCCAGCACCACAAAACCACGATCAGCCCAGGCTTTCTGAATATtc < 1:385883/68‑1 (MQ=255)
ccAGAAACTGGTTACACGGGAATCCCAGCACCACAAAACCACGATCAGCCCAGGCTTTCTGAATATtc < 1:2362070/68‑1 (MQ=255)
ccAGAAACTGGTTACACGGGAATCCCAGCACCACAAAACCACGATCAGCCCAGGCTTTCTGAATATtc < 1:206436/68‑1 (MQ=255)
ccAGAAACTGGTTACACGGGAATCCCAGCACCACAAAACCACGATCAGCCCAGGCTTTCTGAATATtc < 1:3347877/68‑1 (MQ=255)
ccAGAAACTGGTTACACGGGAATCCCAGCACCACAAAACCACGATCAGCCCAGGCTTTCTGAATATtc < 1:954514/68‑1 (MQ=255)
ggTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCt < 1:2891951/69‑1 (MQ=255)
ggTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCt < 1:2861827/69‑1 (MQ=255)
ggTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGATTTCTGAATATTCTCCAACTGCt < 1:2799681/69‑1 (MQ=255)
tGCACGGGAATCCCAGCACCATAAAACCTCGATCGCCCCAGGCTTTCTGAATATTCTCCAACTGCTCa < 1:585735/68‑1 (MQ=255)
tGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCat < 1:1020093/69‑1 (MQ=255)
tGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCat < 1:547664/69‑1 (MQ=255)
tGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCa < 1:1902983/68‑1 (MQ=255)
tGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCa < 1:1924596/68‑1 (MQ=255)
gCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCa < 1:1784506/67‑1 (MQ=255)
gCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCGTTCTGAATATTCTCCAACTGCTCa < 1:1014070/67‑1 (MQ=255)
ggAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTgcg > 1:1051120/1‑69 (MQ=255)
gAATCCCAGCACCACAAAACCACGATCAGCCCAGGc > 1:2183274/1‑36 (MQ=19)
gAATCCCAGCACCACAAAACCACGATCAGCCCAGGCt > 1:1130251/1‑37 (MQ=21)
gAATCCCAGCACCACAAAACCACGATCAGCCCAGGCt > 1:469848/1‑37 (MQ=21)
gAATCCCAGCACCACAAAACCACGATCAGCCCAGGCt > 1:1292487/1‑37 (MQ=21)
gAATCCCAGCACCACAAAACCACGATCAGCCCAGGCt > 1:628020/1‑37 (MQ=21)
gAATCCCAGCACCACAAAACCACGATCAGCCCAGGCt > 1:1296920/1‑37 (MQ=21)
gAATCCCAGCACCACAAAACCACGATCAGCCCAGGCt > 1:1321929/1‑37 (MQ=21)
gAATCCCAGCACCACAAAACCACGATCAGCCCAGGCt > 1:630579/1‑37 (MQ=21)
gAATCCCAGCACCACAAAACCACGATCAGCCCAGGCt > 1:3411250/1‑37 (MQ=21)
gAATCCCAGCACCACAAAACCACGATCAGCCCAGGCt > 1:3395973/1‑37 (MQ=21)
gAATCCCAGCACCACAAAACCACGATCAGCCCAGGCt > 1:630646/1‑37 (MQ=21)
gAATCCCAGCACCACAAAACCACGATCAGCCCAGGCt > 1:3274308/1‑37 (MQ=21)
gAATCCCAGCACCACAAAACCACGATCAGCCCAGGCt > 1:3161735/1‑37 (MQ=21)
gAATCCCAGCACCACAAAACCACGATCAGCCCAGGCt > 1:1582942/1‑37 (MQ=21)
gAATCCCAGCACCACAAAACCACGATCAGCCCAGGCt > 1:2980290/1‑37 (MQ=21)
gAATCCCAGCACCACAAAACCACGATCAGCCCAGGCt > 1:2963872/1‑37 (MQ=21)
gAATCCCAGCACCACAAAACCACGATCAGCCCAGGCt > 1:171521/1‑37 (MQ=21)
gAATCCCAGCACCACAAAACCACGATCAGCCCAGGCt > 1:1835752/1‑37 (MQ=21)
gAATCCCAGCACCACAAAACCACGATCAGCCCAGGCt > 1:1859232/1‑37 (MQ=21)
gAATCCCAGCACCACAAAACCACGATCAGCCCAGGCt > 1:2707523/1‑37 (MQ=21)
gAATCCCAGCACCACAAAACCACGATCAGCCCAGGCt > 1:2576707/1‑37 (MQ=21)
gAATCCCAGCACCACAAAACCACGATCAGCCCAGGCt > 1:2022561/1‑37 (MQ=21)
gAATCCCAGCACCACAAAACCACGATCAGCCCAGGCt > 1:2100877/1‑37 (MQ=21)
ccAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGaa < 1:1735397/39‑1 (MQ=255)
ccAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGaa < 1:2551721/39‑1 (MQ=255)
ccAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGaa < 1:2347281/39‑1 (MQ=255)
ccAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTcc > 1:432808/1‑47 (MQ=255)
ccAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCata > 1:2162895/1‑58 (MQ=255)
ccAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTa > 1:979585/1‑69 (MQ=255)
ccAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTa > 1:2370488/1‑69 (MQ=255)
ccAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTa > 1:2322759/1‑69 (MQ=255)
ccAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTa > 1:1759041/1‑69 (MQ=255)
ccAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTa > 1:1370958/1‑69 (MQ=255)
cATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCcaca < 1:2055692/69‑1 (MQ=255)
cATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCcaca < 1:2035831/69‑1 (MQ=255)
cATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCcaca < 1:1562107/69‑1 (MQ=255)
|
TTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAACTGCTCATATTGCGGCGTTAAGCCACA > minE/1121562‑1121692
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
