Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,178,850 |
G→A |
47.8% |
A100A (GCG→GCA) |
ansA → |
cytoplasmic L‑asparaginase I |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,178,850 | 0 | G | A | 47.8%
| 7.2
/ 82.1
| 94 | A100A (GCG→GCA) | ansA | cytoplasmic L‑asparaginase I |
| Reads supporting (aligned to +/- strand): ref base G (12/37); new base A (19/26); total (31/63) |
| Fisher's exact test for biased strand distribution p-value = 8.15e-02 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
ATGACGACTATGATGGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTG > minE/1178786‑1178917
|
aTGACGACTATGATGGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCt > 1:215505/1‑67 (MQ=255)
aTGACGACTATGATGGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGt > 1:2486516/1‑69 (MQ=255)
atgGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGt < 1:1639198/60‑1 (MQ=255)
atgGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGt < 1:2257998/60‑1 (MQ=255)
atgGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGt < 1:2036724/60‑1 (MQ=255)
atgGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGt < 1:2634533/60‑1 (MQ=255)
atgGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGt < 1:2973523/60‑1 (MQ=255)
atgGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGt < 1:557825/60‑1 (MQ=255)
atgGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGt < 1:1435850/60‑1 (MQ=255)
tttGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGaa < 1:2758563/68‑1 (MQ=255)
tttGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGaa < 1:993890/68‑1 (MQ=255)
tttGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGaa < 1:850055/68‑1 (MQ=255)
tttGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGaa < 1:2028356/68‑1 (MQ=255)
tttGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGaa < 1:230257/68‑1 (MQ=255)
tttGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGaa < 1:1176718/68‑1 (MQ=255)
tttGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGaa < 1:637998/68‑1 (MQ=255)
tttGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGaa < 1:3395173/68‑1 (MQ=255)
ttGTCATTCTGCACGGTACAGACACGATGGCGTATACCGCCTCTGCACTGTCGTTCATGCTCGAGAAtc > 1:495347/1‑69 (MQ=255)
ttGTCATTCTGCACGGTACAGACACGATGGCGTATACCGCCTCTGCACTGTCGTTCATGCTCGAGAAtc > 1:1498042/1‑69 (MQ=255)
ttGTCATTCTGCACGGTACAGACACGATGGCGTATACCGCCTCTGCACTGTCGTTCATGCTCGAGAAtc > 1:804464/1‑69 (MQ=255)
ttGTCATTCTGCACGGTACAGACACGATGGCGTATACCGCCTCTGCACTGTCGTTCATGCTCGAGAAtc > 1:1963260/1‑69 (MQ=255)
ttGTCATTCTGCACGGTACAGACACGATGGCGTATACCGCCTCTGCACTGTCGTTCATGCTCGAGAAtc > 1:1948663/1‑69 (MQ=255)
ttGTCATTCTGCACGGTACAGACACGATGGCGTATACCGCCTCTGCACTGTCGTTCATGCTCGAGAAtc > 1:819074/1‑69 (MQ=255)
ttGTCATTCTGCACGGTACAGACACGATGGCGTATACCGCCTCTGCACTGTCGTTCATGCTCGAGAAtc > 1:243729/1‑69 (MQ=255)
ttGTCATTCTGCACGGTACAGACACGATGGCGTATACCGCCTCTGCACTGTCGTTCATGCTCGAGAAtc > 1:2483896/1‑69 (MQ=255)
ttGTCATTCTGCACGGTACAGACACGATGGCGTATACCGCCTCTGCACTGTCGTTCATGCTCGAGAAtc > 1:2559906/1‑69 (MQ=255)
ttGTCATTCTGCACGGTACAGACACGATGGCGTATACCGCCTCTGCACTGTCGTTCATGCTCGAGAAtc > 1:120273/1‑69 (MQ=255)
ttGTCATTCTGCACGGTACAGACACGATGGCGTATACCGCCTCTGCACTGTCGTTCATGCTCGAGAAtc > 1:1619155/1‑69 (MQ=255)
ttGTCATTCTGCACGGTACAGACACGATGGCGTATACCGCCTCTGCACTGTCGTTCATGCTCGAGAAtc > 1:838764/1‑69 (MQ=255)
ttGTCATTCTGCACGGTACAGACACGATGGCGTATACCGCCTCTGCACTGTCGTTCATGCTCGAGAAtc > 1:3141321/1‑69 (MQ=255)
ttGTCATTCTGCACGGTACAGACACGATGGCGTATACCGCCTCTGCACTGTCGTTCATGCTCGAGAAtc > 1:1353197/1‑69 (MQ=255)
ttGTCATTCTGCACGGTACAGACACGATGGCGTATACCGCCTCTGCACTGTCGTTCATGCTCGAGAAt > 1:2197769/1‑68 (MQ=255)
ttGTCATTCTGCACGGTACAGACACGATGGCGTATACCGCCTCTGAACTGTCGTTCATGCTCGAGAAt > 1:3285111/1‑68 (MQ=255)
ttGTCATTCTGCACGGTACAGACACGATGGCGTATACCGCATCTGCACTGTCGTTCATGCTCGAGAAtc > 1:2070818/1‑69 (MQ=255)
ttGTCATTATGCACGGTACAGACACGATGGCGTATACCGCCTCTGCACTGTCGTTCATGCTCGAGaa > 1:2565139/1‑67 (MQ=255)
atGTCATTCTGCACGGTACAGACACGATGGCGTATACCGCCTCTGCACTGTCGTTCATGc > 1:2305838/2‑60 (MQ=255)
cATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGt < 1:173979/50‑1 (MQ=255)
cGGTACAGACACGATGGCGTATACCGCCTCTGCACTGTCGTTCATGCTCGAGAATCTCGGTAAACCg < 1:514743/67‑1 (MQ=255)
cGGTACAGACACGATGGCGTATACCGCCTCTGCACTGTCGTTCATGCTCGAGAATCTCGGTAAACCg < 1:3050909/67‑1 (MQ=255)
cGGTACAGACACGATGGCGTATACCGCCTCTGCACTGTCGTTCATGCTCGAGAATCTCGGTAAACCg < 1:1727794/67‑1 (MQ=255)
cGGTACAGACACGATGGCGTATACCGCCTCTGCACTGTCGTTCATGCTCGAGAATCTCGGTAAACCg < 1:3362331/67‑1 (MQ=255)
cGGTACAGACACGATGGCGTATACCGCCTCTGCACTGTCGTTCATGCTCGAGAATCTCGGTAAACCg < 1:3379424/67‑1 (MQ=255)
cGGTACAGACACGATGGCGTATACCGCCTCTGCACTGTCGTTCATGCTCGAGAATCTCGGTAAACCg < 1:351666/67‑1 (MQ=255)
cGGTACAGACACGATGGCGTATACCGCCTCTGCACTGTCGTTCATGCTCGAGAATCTCGGTAAACCg < 1:353679/67‑1 (MQ=255)
cGGTACAGACACGATGGCGTATACCGCCTCTGCACTGTCGTTCATGCTCGAGAATCTCGGTAAACCg < 1:395790/67‑1 (MQ=255)
cGGTACAGACACGATGGCGTATACCGCCTCTGCACTGTCGTTCATGCTCGAGAATCTCGGTAAACCg < 1:502669/67‑1 (MQ=255)
cGGTACAGACACGATGGCGTATACCGCCTCTGCACTGTCGTTCATGCTCGAGAATCTCGGTAAACCg < 1:2169870/67‑1 (MQ=255)
cGGTACAGACACGATGGCGTATACCGCCTCTGCACTGTCGTTCATGCTCGAGAATCTCGGTAAACCg < 1:531040/67‑1 (MQ=255)
cGGTACAGACACGATGGCGTATACCGCCTCTGCACTGTCGTTCATGCTCGAGAATCTCGGTAAACCg < 1:541920/67‑1 (MQ=255)
cGGTACAGACACGATGGCGTATACCGCCTCTGCACTGTCGTTCATGCTCGAGAATCTCGGTAAACCg < 1:157458/67‑1 (MQ=255)
cGGTACAGACACGATGGCGTATACCGCCTCTGCACTGTCGTTCATGCTCGAGAATCTCGGTAAACCg < 1:605519/67‑1 (MQ=255)
cGGTACAGACACGATGGCGTATACCGCCTCTGCACTGTCGTTCATGCTCGAGAATCTCGGTAAACCg < 1:1072775/67‑1 (MQ=255)
cGGTACAGACACGATGGCGTATACCGCCTCTGCACTGTCGTTCATGCTCGAGAATCTCGGTAAACCg < 1:899626/67‑1 (MQ=255)
cGGTACAGACACGATGGCGTATACCGCCTCTGCACTGTCGTTCATGCTCGAGAATCTCGGTAAACCg < 1:105127/67‑1 (MQ=255)
cGGTACAGACACGATGGCGTATACCGCCTCTGCACTGTCGTTCATGCTCGAGAATCTCGGTAAACCg < 1:2190353/67‑1 (MQ=255)
cGGTACAGACACGATGGCGTATACCGCCTCTGCACTGTCGTTCATGCTCGAGAATCTCGGTAAACCg < 1:236701/67‑1 (MQ=255)
cGGTACAGACACGATGGCGTATACCGCCTCTGCACTGTCGTTCATGCTCGAGAATCTCGGTAAACCg < 1:1919565/67‑1 (MQ=255)
cGGTACAGACACGATGGCGTATACCGCCTCTGCACTGTCGTTCATGCTCGAGAATCTCGGTAAACCg < 1:248783/67‑1 (MQ=255)
cGGTACAGACACGATGGCGTATACCGCCTCTGCACTGTCGTTCATGCTCGAGAATCTCGGTAAACCg < 1:1883401/67‑1 (MQ=255)
cGGTACAGACACGATGGCGTATACCGCCTCTGCACTGTCGTTCATGCTCGAGAATCTCGGTAAACCg < 1:2629084/67‑1 (MQ=255)
cGGTACAGACACGATGGCGTATACCGCCTCTGCACTGTCGTTCATGCTCGAGAATCTCGGTAAACCg < 1:2645369/67‑1 (MQ=255)
cGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGt > 1:1936082/1‑69 (MQ=255)
cACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCAt < 1:369061/69‑1 (MQ=255)
cACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCAt < 1:1267678/69‑1 (MQ=255)
cACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCAt < 1:1505281/69‑1 (MQ=255)
cACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCAt < 1:1845956/69‑1 (MQ=255)
cACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCAt < 1:3310876/69‑1 (MQ=255)
cACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCAt < 1:2949516/69‑1 (MQ=255)
cACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCAt < 1:2767495/69‑1 (MQ=255)
aCCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCAt < 1:269187/68‑1 (MQ=255)
tataCCGCCTCTGCACTGTCGTTCATGCTCGAGAATCTCGGTAAACCg < 1:1813262/48‑1 (MQ=255)
ccGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATcc > 1:2661291/1‑69 (MQ=255)
ccGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATcc > 1:1498439/1‑69 (MQ=255)
ccGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATcc > 1:43005/1‑69 (MQ=255)
ccGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATcc > 1:1596406/1‑69 (MQ=255)
ccGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATc > 1:2323726/1‑68 (MQ=255)
ctctTCACTGTCGTTCATGCTCGAGAATCTCGGTAAACCg < 1:3385929/40‑1 (MQ=38)
tGCGCTGTCTTTCATGCTCGAGAATCTCGGTACACCGGt < 1:441874/39‑1 (MQ=37)
tGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGt < 1:1387520/39‑1 (MQ=255)
tGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGt < 1:1890368/39‑1 (MQ=255)
tGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGt < 1:1886441/39‑1 (MQ=255)
tGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGt < 1:3026054/39‑1 (MQ=255)
tGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGt < 1:2549456/39‑1 (MQ=255)
tGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGt < 1:391623/39‑1 (MQ=255)
tGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGt < 1:2767888/39‑1 (MQ=255)
tGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGt < 1:1622862/39‑1 (MQ=255)
tGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGt < 1:3118564/39‑1 (MQ=255)
tGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGt < 1:2037762/39‑1 (MQ=255)
tGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTcaca > 1:3152890/1‑57 (MQ=255)
tGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTcaca > 1:2732548/1‑57 (MQ=255)
tGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTcaca > 1:1025974/1‑57 (MQ=255)
tGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTcaca > 1:3209998/1‑57 (MQ=255)
tGCGCTGTAGTTCATGCTCGAGAATCTCGGTAAACCGGt < 1:181879/39‑1 (MQ=255)
tGCGCTGGCGTTCATGCTCGAGAATCTCGGTAAACCGGt < 1:398099/39‑1 (MQ=255)
cgcTGTCGTTCATGCTCGATAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCgctggctg > 1:298175/1‑69 (MQ=255)
cgcTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCgctggctg > 1:60775/1‑69 (MQ=255)
cgcTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCgctggctg > 1:651637/1‑69 (MQ=255)
|
ATGACGACTATGATGGTTTTGTCATTCTGCACGGCACCGACACGATGGCGTATACCGCCTCTGCGCTGTCGTTCATGCTCGAGAATCTCGGTAAACCGGTCATTGTGACAGGGTCACAAATCCCGCTGGCTG > minE/1178786‑1178917
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
