Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,182,072 |
C→T |
53.6% |
V231V (GTG→GTA) |
yeaE ← |
predicted oxidoreductase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,182,072 | 0 | C | T | 53.6%
| 8.8
/ 81.3
| 96 | V231V (GTG→GTA) | yeaE | predicted oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base C (26/18); new base T (46/5); total (73/23) |
| Fisher's exact test for biased strand distribution p-value = 6.06e-04 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGC > minE/1182004‑1182137
|
gCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATc < 1:2064171/69‑1 (MQ=255)
cATTTTGTTGGACATGGGCAATCGTTGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCAcc < 1:912661/68‑1 (MQ=255)
ggACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCcaac < 1:971767/68‑1 (MQ=255)
ggACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCcaac < 1:1909594/68‑1 (MQ=255)
ggACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCcaac < 1:911187/68‑1 (MQ=255)
ggACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCcaac < 1:90339/68‑1 (MQ=255)
gACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCcaacaa > 1:1637854/1‑69 (MQ=255)
gACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCcaacaa > 1:175690/1‑69 (MQ=255)
gACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCcaacaa > 1:1500604/1‑69 (MQ=255)
gACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCcaacaa > 1:2879435/1‑69 (MQ=255)
gACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCcaacaa > 1:1101961/1‑69 (MQ=255)
gACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCcaaca > 1:3415566/1‑68 (MQ=255)
gTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCcaacaa < 1:334881/56‑1 (MQ=255)
gTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCcaacaa < 1:1442589/56‑1 (MQ=255)
gTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCcaacaa < 1:776633/56‑1 (MQ=255)
gTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCcaacaa < 1:2869238/56‑1 (MQ=255)
gTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCcaacaa < 1:1785841/56‑1 (MQ=255)
gTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCcgcg > 1:3379011/1‑69 (MQ=255)
gTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCAAGCcaacaa < 1:2305334/56‑1 (MQ=255)
tgCCGCTTTTGGGATCGCCATCACACCCTGATGGCTGATTACCCACGCCAACAATACTTGTGc < 1:1032153/62‑1 (MQ=255)
cGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACTCCAACAATACTTGTGCCGCGCTGa < 1:1219626/68‑1 (MQ=255)
cGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGa < 1:3305028/68‑1 (MQ=255)
cGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGa < 1:3301035/68‑1 (MQ=255)
cGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGa < 1:97159/68‑1 (MQ=255)
cGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGa < 1:1681913/68‑1 (MQ=255)
cGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGa < 1:1015311/68‑1 (MQ=255)
cGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAACACTTGTGCCGCGCTGa < 1:3018766/68‑1 (MQ=255)
tCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCAt > 1:740929/1‑69 (MQ=255)
tCACACCCTGATGGCTGATTACCCACGCCAACAATACTTGTGCCGCACTGATATTGTGAGCATGTGCaa > 1:1458614/1‑69 (MQ=255)
tCACACCCTGATGGCTGATTACCCACGCCAACAATACTTGTGCCGCACTGATATTGTGAGCATGTGCaa > 1:2707130/1‑69 (MQ=255)
tCACACCCTGATGGCTGATTACCCACGCCAACAATACTTGTGCCGCACTGATATTGTGAGCATGTGCaa > 1:2777391/1‑69 (MQ=255)
tCACACCCTGATGGCTGATTACCCACGCCAACAATACTTGTGCCGCACTGATATTGTGAGCATGTGCaa > 1:2822273/1‑69 (MQ=255)
tCACACCCTGATGGCTGATTACCCACGCCAACAATACTTGTGCCGCACTGATATTGTGAGCATGTGCaa > 1:2825852/1‑69 (MQ=255)
tCACACCCTGATGGCTGATTACCCACGCCAACAATACTTGTGCCGCACTGATATTGTGAGCATGTGCaa > 1:2931262/1‑69 (MQ=255)
tCACACCCTGATGGCTGATTACCCACGCCAACAATACTTGTGCCGCACTGATATTGTGAGCATGTGCaa > 1:2962113/1‑69 (MQ=255)
tCACACCCTGATGGCTGATTACCCACGCCAACAATACTTGTGCCGCACTGATATTGTGAGCATGTGCaa > 1:3098987/1‑69 (MQ=255)
tCACACCCTGATGGCTGATTACCCACGCCAACAATACTTGTGCCGCACTGATATTGTGAGCATGTGCaa > 1:878694/1‑69 (MQ=255)
tCACACCCTGATGGCTGATTACCCACGCCAACAATACTTGTGCCGCACTGATATTGTGAGCATGTGCaa > 1:2880842/1‑69 (MQ=255)
tCACACCCTGATGGCTGATTACCCACGCCAACAATACTTGTGCCGCACTGATATTGTGAGCATGTGCaa > 1:1416263/1‑69 (MQ=255)
tCACACCCTGATGGCTGATTACCCACGCCAACAATACTTGTGCCGCACTGATATTGTGAGCATGTGCaa > 1:3336257/1‑69 (MQ=255)
tCACACCCTGATGGCTGATTACCCACGCCAACAATACTTGTGCCGCACTGATATTGTGAGCATGTGCaa > 1:3336969/1‑69 (MQ=255)
tCACACCCTGATGGCTGATTACCCACGCCAACAATACTTGTGCCGCACTGATATTGTGAGCATGTGCaa > 1:1375250/1‑69 (MQ=255)
tCACACCCTGATGGCTGATTACCCACGCCAACAATACTTGTGCCGCACTGATATTGTGAGCATGTGCaa > 1:822879/1‑69 (MQ=255)
tCACACCCTGATGGCTGATTACCCACGCCAACAATACTTGTGCCGCACTGATATTGTGAGCATGTGCaa > 1:3410226/1‑69 (MQ=255)
tCACACCCTGATGGCTGATTACCCACGCCAACAATACTTGTGCCGCACTGATATTGTGAGCATGTGCaa > 1:76004/1‑69 (MQ=255)
tCACACCCTGATGGCTGATTACCCACGCCAACAATACTTGTGCCGCACTGATATTGTGAGCATGTGCaa > 1:655507/1‑69 (MQ=255)
tCACACCCTGATGGCTGATTACCCACGCCAACAATACTTGTGCCGCACTGATATTGTGAGCATGTGCaa > 1:104812/1‑69 (MQ=255)
tCACACCCTGATGGCTGATTACCCACGCCAACAATACTTGTGCCGCACTGATATTGTGAGCATGTGCaa > 1:944733/1‑69 (MQ=255)
tCACACCCTGATGGCTGATTACCCACGCCAACAATACTTGTGCCGCACTGATATTGTGAGCATGTGCaa > 1:2011405/1‑69 (MQ=255)
tCACACCCTGATGGCTGATTACCCACGCCAACAATACTTGTGCCGCACTGATATTGTGAGCATGTGCaa > 1:1498737/1‑69 (MQ=255)
tCACACCCTGATGGCTGATTACCCACGCCAACAATACTTGTGCCGCACTGATATTGTGAGCATGTGCaa > 1:2182983/1‑69 (MQ=255)
tCACACCCTGATGGCTGATTACCCACGCCAACAATACTTGTGCCGCACTGATATTGTGAGCATGTGCaa > 1:1568722/1‑69 (MQ=255)
tCACACCCTGATGGCTGATTACCCACGCCAACAATACTTGTGCCGCACTGATATTGTGAGCATGTGCaa > 1:2469043/1‑69 (MQ=255)
tCACACCCTGATGGCTGATTACCCACGCCAACAATACTTGTGCCGCACTGATATTGTGAGCATGTGCaa > 1:1216550/1‑69 (MQ=255)
tCACACCCTGATGGCTGATTACCCACGCCAACAATACTTGTGCCGCACTGATATTGTGAGCATGTGCa > 1:41834/1‑68 (MQ=255)
acacCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAAtt > 1:565678/1‑69 (MQ=255)
acacCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAAtt > 1:686866/1‑69 (MQ=255)
acacCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAAtt > 1:1684448/1‑69 (MQ=255)
acacCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAAtt > 1:2618102/1‑69 (MQ=255)
acacCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAAtt > 1:132916/1‑69 (MQ=255)
acacCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAAtt > 1:583037/1‑69 (MQ=255)
acacCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAAtt > 1:618090/1‑69 (MQ=255)
acacCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAAtt > 1:1827685/1‑69 (MQ=255)
acacCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAAtt > 1:662101/1‑69 (MQ=255)
acacCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAAtt > 1:918920/1‑69 (MQ=255)
acacCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAAtt > 1:2965320/1‑69 (MQ=255)
acacCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAAtt > 1:2519285/1‑69 (MQ=255)
acacCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAAtt > 1:2460942/1‑69 (MQ=255)
acacCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAAtt > 1:1492857/1‑69 (MQ=255)
acacCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAAtt > 1:2214367/1‑69 (MQ=255)
acacCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAAtt > 1:2937524/1‑69 (MQ=255)
ggcTGATTACCCACGCCAACAATACTTGTGCCGCACTGATATTGTGAGCATGTGCAATTTCGTTGAc < 1:2074142/65‑1 (MQ=255)
ggcTGATTACCCACGCCAACAATACTTGTGCCGCACTGATATTGTGAGCATGTGCAATTTCGTTGAc < 1:778585/65‑1 (MQ=255)
ggcTGATTACCCACGCCAACAATACTTGTGCCGCACTGATATTGTGAGCATGTGCAATTTCGTTGAc < 1:782368/65‑1 (MQ=255)
ggcTGATTACCCACGCCAACAATACTTGTGCCGCACTGATATTGTGAGCATGTGCAATTTCGTTGAc < 1:1576147/65‑1 (MQ=255)
gaCTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTa > 1:1219693/1‑69 (MQ=255)
gaCTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTa > 1:3360401/1‑69 (MQ=255)
gATTACCCACGCCAACAATACTTGTGCCGCACTGATATTGTGAGCATGTGCAATTTCGTTGACTACcgc > 1:960354/1‑69 (MQ=255)
gATTACCCACGCCAACAATACTTGTGCCGCACTGATATTGTGAGCATGTGCAATTTCGTTGACTACcgc > 1:1015166/1‑69 (MQ=255)
gATTACCCACGCCAACAATACTTGTGCCGCACTGATATTGTGAGCATGTGCAATTTCGTTGACTACcgc > 1:909505/1‑69 (MQ=255)
gATTACCCACGCCAACAATACTTGTGCCGCACTGATATTGTGAGCATGTGCAATTTCGTTGACTACcgc > 1:1094001/1‑69 (MQ=255)
gATTACCCACGCCAACAATACTTGTGCCGCACTGATATTGTGAGCATGTGCAATTTCGTTGACTACcgc > 1:1613059/1‑69 (MQ=255)
gATTACCCACGCCAACAATACTTGTGCCGCACTGATATTGTGAGCATGTGCAATTTCGTTGACTACcgc > 1:1472788/1‑69 (MQ=255)
gATTACCCACGCCAACAATACTTGTGCCGCACTGATATTGTGAGCATGTGCAATTTCGTTGACTACcgc > 1:1598507/1‑69 (MQ=255)
gATTACCCACGCCAACAATACTTGTGCCGCACTGATATTGTGAGCATGTGCAATTTCGTTGACTACcgc > 1:1740064/1‑69 (MQ=255)
gATTACCCACGCCAACAATACTTGTGCCGCACTGATATTGTGAGCATGTGCAATTTCGTTGACTACcgc > 1:1750411/1‑69 (MQ=255)
gATTACCCACGCCAACAATACTTGTGCCGCACTGATATTGTGAGCATGTGCAATTTCGTTGACTACcgc > 1:1534049/1‑69 (MQ=255)
gATTACCCACGCCAACAATACTTGTGCCGCACTGATATTGTGAGCATGTGCAATTTCGTTGACTACcgc > 1:2473201/1‑69 (MQ=255)
gATTACCCACGCCAACAATACTTGTGCCGCACTGATATTGTGAGCATGTGCAATTTCGTTGACTACcgc > 1:1476648/1‑69 (MQ=255)
gATTACCCACGCCAACAATACTTGTGCCGCACTGATATTGTGAGCATGTGCAATTTCGTTGACTACcgc > 1:3264033/1‑69 (MQ=255)
gATTACCCACGCCAACAATACTTGTGCCGCACTGATATTGTGAGCATGTGCAATTTCGTTGACTACcgc > 1:1358801/1‑69 (MQ=255)
gATTACCCACGCCAACAATACTTGTGCCGCACTGATATTGTGAGCATGTGCAATTTCGTTGACTACcgc > 1:3420392/1‑69 (MQ=255)
gATTACCCACGCCAACAATACTTGTGCCGCACTGATATTGTGAGCATGTGCAATTTCGTTGACTACcgc > 1:422723/1‑69 (MQ=255)
gATTACCCACGCCAACAATACTTGTGCCGCACTGATATTGTGAGCATGTGCAATTTCGTTGACTACcgc > 1:511373/1‑69 (MQ=255)
gATTACCCACGCCAACAATACTTGTGCCGCACTGATATTGTGAGCATGTGCAATTTCGTTGACTACcgc > 1:548558/1‑69 (MQ=255)
gATTACCCACGACAACAATACTTGTGCCGCACTGATATTGTGAGCATGTGCAATTTCGTTGACTACcgc > 1:382444/1‑69 (MQ=255)
gATAACCCACGCCAACAATACTTGTGCCGCACTGatat > 1:64642/1‑38 (MQ=38)
cACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGc > 1:2379668/1‑48 (MQ=255)
cACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGc > 1:1242873/1‑48 (MQ=255)
cACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGc > 1:1904559/1‑48 (MQ=255)
|
GCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGC > minE/1182004‑1182137
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
