Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,248,433 |
A→C |
67.3% |
P280P (CCT→CCG) |
ruvB ← |
ATP‑dependent DNA helicase, component of RuvABC resolvasome |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,248,433 | 0 | A | C | 67.3%
| 56.4
/ 31.8
| 52 | P280P (CCT→CCG) | ruvB | ATP‑dependent DNA helicase, component of RuvABC resolvasome |
| Reads supporting (aligned to +/- strand): ref base A (6/11); new base C (30/5); total (36/16) |
| Fisher's exact test for biased strand distribution p-value = 4.20e-04 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.51e-01 |
GTTTCACGTTCTTCGCCAATGGCTGCCGCCAGGTTATCCAGACCTACAGGTCCACCAAAGAACTTATCGATTACCGCCAGCAACAATTTGCGGTCCATATAATCGAAA > minE/1248386‑1248493
|
gTTTCACGTTCTTCGCCAATGGCTGCCGCCAGGTTATCCAGACCTACAGGTCCACCAAAGAACTTATCg > 1:103916/1‑69 (MQ=255)
cttcGCCAATGGCTGCCGCCAGGTTATCCAGACCTACAGGTCCACCAAAGAACTTATCGATTACCGcc > 1:118898/1‑68 (MQ=255)
cttcGCCAATGGCTGCCGCCAGGTTATCCAGACCTACAGGTCCACCAAAGAACTTATCGATTACCGcc > 1:552473/1‑68 (MQ=255)
aTGGCTGCCGCCAGGTTATCCAGACCTACAGGTCCACCAAAGAACTTATCGAt > 1:2784318/1‑53 (MQ=255)
aTGGCTGCCGCCAGGTTATCCAGACCTACAGGTCCACCAAAGAACTTATCGAt > 1:1626930/1‑53 (MQ=255)
aTGGCTGCCGCCAGCTTATCCAGACCTACAGGTCCACCAAAGAACTTATCGAt > 1:81738/1‑53 (MQ=255)
gCTGCCGCCAGGTTATCCAGACCTACAGGTCCACCAAAGAACTTATc < 1:2872487/47‑1 (MQ=255)
cTGCCGCCAGGTTATCCAGACCTACAGGTCCACCAAAGAACTTATc < 1:1717581/46‑1 (MQ=255)
ccgccAGGTTATCCAGACCTACCGGCCCACCAAAGAACTTATCGATTACCGCCAGCAACAATTTGCgg > 1:2804603/1‑68 (MQ=255)
ccgccAGGTTATCCAGACCTACCGGCCCACCAAAGAACTTATCGATTACCGCCAGCAACAATTTGCgg > 1:3257700/1‑68 (MQ=255)
ccgccAGGTTATCCAGACCTACCGGCCCACCAAAGAACTTATCGATTACCGCCAGCAACAATTTGCgg > 1:3308060/1‑68 (MQ=255)
ccgccAGGTTATCCAGACCTACCGGCCCACCAAAGAACTTATCGATTACCGCCAGCAACAATTTGCgg > 1:2059076/1‑68 (MQ=255)
ccgccAGGTTATCCAGACCTACCGGCCCACCAAAGAACTTATCGATTACCGCCAGCAACAATTTGCgg > 1:362832/1‑68 (MQ=255)
ccgccAGGTTATCCAGACCTACCGGCCCACCAAAGAACTTATCGATTACCGCCAGCAACAATTTGCgg > 1:1930380/1‑68 (MQ=255)
ccgccAGGTTATCCAGACCTACCGGCCCACCAAAGAACTTATCGATTACCGCCAGCAACAATTTGCgg > 1:1633426/1‑68 (MQ=255)
ccgccAGGTTATCCAGACCTACCGGCCCACCAAAGAACTTATCGATTACCGCCAGCAACAATTTGCgg > 1:425629/1‑68 (MQ=255)
ccgccAGGTTATCCAGACCTACCGGCCCACCAAAGAACTTATCGATTACCGCCAGCAACAATTTGCgg > 1:485973/1‑68 (MQ=255)
ccgccAGGTTATCCAGACCTACCGGCCCACCAAAGAACTTATCGATTACCGCCAGCAACAATTTGCgg > 1:516421/1‑68 (MQ=255)
ccgccAGGTTATCCAGACCTACCGGCCCACCAAAGAACTTATCGATTACCGCCAGCAACAATTTGCgg > 1:662687/1‑68 (MQ=255)
ccgccAGGTTATCCAGACCTACCGGCCCACCAAAGAACTTATCGATTACCGCCAGCAACAATTTGCgg > 1:784987/1‑68 (MQ=255)
ccgccAGGTTATCCAGACCTACCGGCCCACCAAAGAACTTATCGATTACCGCCAGCAACAATTTGCg > 1:2265954/1‑67 (MQ=255)
ccgccAGGTTATCCAGACCTACCGGCCCACCAAAGAACTAATCGATTACCGCCAGCAACAATTTGCgg > 1:2556752/1‑68 (MQ=255)
ccAGACCTACCGGCCCACCAAAGAACTTATCGATTACCGCCAGCAACAAttt > 1:36410/1‑52 (MQ=255)
ccAGACCTACCGGCCCACCAAAGAACTTATCGATTACCGCCAGCAACAATTTGCGGTCCATATAATCGa > 1:388313/1‑69 (MQ=255)
ccAGACCTACCGGCCCACCAAAGAACTTATCGATTACCGCCAGCAACAATTTGCGGTCCATATAATCGa > 1:3391706/1‑69 (MQ=255)
ccAGACCTACCGGCCCACCAAAGAACTTATCGATTACCGCCAGCAACAATTTGCGGTCCATATAATCGa > 1:3390850/1‑69 (MQ=255)
ccAGACCTACCGGCCCACCAAAGAACTTATCGATTACCGCCAGCAACAATTTGCGGTCCATATAATCGa > 1:3360846/1‑69 (MQ=255)
ccAGACCTACCGGCCCACCAAAGAACTTATCGATTACCGCCAGCAACAATTTGCGGTCCATATAATCGa > 1:2983956/1‑69 (MQ=255)
ccAGACCTACCGGCCCACCAAAGAACTTATCGATTACCGCCAGCAACAATTTGCGGTCCATATAATCGa > 1:1043090/1‑69 (MQ=255)
ccAGACCTACCGGCCCACCAAAGAACTTATCGATTACCGCCAGCAACAATTTGCGGTCCATATAATCGa > 1:2767501/1‑69 (MQ=255)
ccAGACCTACCGGCCCACCAAAGAACTTATCGATTACCGCCAGCAACAATTTGCGGTCCATATAATCGa > 1:1083724/1‑69 (MQ=255)
ccAGACCTACCGGCCCACCAAAGAACTTATCGATTACCGCCAGCAACAATTTGCGGTCCATATAATCGa > 1:1302741/1‑69 (MQ=255)
ccAGACCTACCGGCCCACCAAAGAACTTATCGATTACCGCCAGCAACAATTTGCGGTCCATATAATCGa > 1:1417199/1‑69 (MQ=255)
ccAGACCTACCGGCCCACCAAAGAACTTATCGATTACCGCCAGCAACAATTTGCGGTCCATATAATCGa > 1:2701282/1‑69 (MQ=255)
ccAGACCTACCGGCCCACCAAAGAACTTATCGATTACCGCCAGCAACAATTTGCGGTCCATATAATCGa > 1:1535007/1‑69 (MQ=255)
ccAGACCTACCGGCCCACCAAAGAACTTATCGATTACCGCCAGCAACAATTTGCGGTCCATATAATCGa > 1:1723396/1‑69 (MQ=255)
ccAGACCTACCGGCCCACCAAAGAACTTATCGATTACCGCCAGCAACAATTTGCGGTCCATATAATCGa > 1:2064971/1‑69 (MQ=255)
ccAGACCTACCGGCCCACCAAAGAACTTATAGATAACCGCCAGcaac > 1:1412103/1‑47 (MQ=37)
cAGACCTACCGGCCCACCAAAGAACTTATCGATTACCGCCAGCAACAATTTGCGGTCCATATAATCGaa < 1:1957110/69‑1 (MQ=255)
cAGACCTACCGGCCCACCAAAGAACTTATCGATTACCGCCAGCAACAATTTGCGGTCCATATAATCGaa < 1:2158973/69‑1 (MQ=255)
cAGACCTACCGGCCCACCAAAGAACTTATCGATTACCGCCAGCAACAATTTGCGGTCCATATAATCGaa < 1:2242019/69‑1 (MQ=255)
cAGACCTACCGGCCCACCAAAGAACTTATCGATTACCGCCAGCAACAATTTGCGGTCCATATAATCGaa < 1:2614013/69‑1 (MQ=255)
cAGACCTACCGGCCCACCAAAGAACTTATCGATTACCGCCAGCAACAATTTGCGGTCCATATAATCGaa < 1:2965184/69‑1 (MQ=255)
cAGACCTACAGGTCCACCAAAGAACTTATCGATTACCGCCAGCAACAATTTGCGGTCCATATAATCGa < 1:2823590/68‑1 (MQ=255)
cAGACCTACAGGTCCACCAAAGAACTTATCGATTACCGCCAGCAACAATTTGCGGTCCATATAATCGa < 1:1633789/68‑1 (MQ=255)
cAGACCTACAGGTCCACCAAAGAACTTATCGATTACCGCCAGCAACAATTTGCGGTCCATATAATCGa < 1:3037999/68‑1 (MQ=255)
cAGACCTACAGGTCCACCAAAGAACTTATCGATTACCGCCAGCAACAATTTGCGGTCCATATAATCGa < 1:1415028/68‑1 (MQ=255)
gACCTACAGGTCCACCAAAGAACTTATCGATTACCGCCAGCAACAATTTGCGGTCCATATAATCGaaa < 1:503262/68‑1 (MQ=255)
gACCTACAGGTCCACCAAAGAACTTATCGATTACCGCCAGCAACAATTTGCGGTCCATATAATCGaaa < 1:2729972/68‑1 (MQ=255)
gACCTACAGGTCCACCAAAGAACTTATCGATTACCGCCAGCAACAATTTGCGGTCCATATAATCGaaa < 1:1186453/68‑1 (MQ=255)
gACCTACAGGTCCACCAAAGAACTTATCGATTACCGCCAGCAACAATTTGCGGTCCATATAATCGaaa < 1:2912393/68‑1 (MQ=255)
gACCTACAGGTCCACCAAAGAACTTATCGATTACCGCCAGCAACAATTTGCGGTCCATATAATCGaaa < 1:973018/68‑1 (MQ=255)
|
GTTTCACGTTCTTCGCCAATGGCTGCCGCCAGGTTATCCAGACCTACAGGTCCACCAAAGAACTTATCGATTACCGCCAGCAACAATTTGCGGTCCATATAATCGAAA > minE/1248386‑1248493
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
