Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,293,854 |
G→C |
34.6% |
L124L (CTC→CTG) |
cbl ← |
DNA‑binding transcriptional activator |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,293,854 | 0 | G | C | 34.6%
| 71.5
/ 58.3
| 75 | L124L (CTC→CTG) | cbl | DNA‑binding transcriptional activator |
| Reads supporting (aligned to +/- strand): ref base G (15/34); new base C (16/10); total (31/44) |
| Fisher's exact test for biased strand distribution p-value = 1.38e-02 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
ACCAATATCAGCTTCGCCATTTTGCAACAATGTCGCAATTTCCTGTGGCGTCCCCTGGATTAGCTCGAGCCGAACCTCCGGGAAAAGTTCGCGAAAAGCTTTAATGACCTCTGGCAAGCTATAACGTGCCTGAG > minE/1293788‑1293921
|
acgaaTATCAGCTTCGCCATTTTGCAACAATGTCGCAATTTCCTGTGGCGTCCCCTGGATTAGCTCGAg > 1:720523/4‑69 (MQ=255)
aCCAATATCAGCTTCGCCATTTTGCAACAATGTCGCAATTTCCTTTGGCGTCCCCTGGATTAGCTCGAg > 1:2608954/1‑69 (MQ=255)
aCCAATATCAGCTTCGCCATTTTGCAACAATGTCGCAATTTCCTGTGGCGTCCCCTGGATTAGCTCgg > 1:3281865/1‑67 (MQ=255)
aCCAATATCAGCTTCGCCATTTTGCAACAATGTCGCAATTTCCTGTGGCGTCCCCTGGATTAGCTCGAg > 1:3037754/1‑69 (MQ=255)
aCCAATATCAGCTTCGCCATTTTGCAACAATGTCGCAATTTCCTGTGGCGTCCCCTGGATTAGCTCGAg > 1:3108470/1‑69 (MQ=255)
aCCAATATCAGCTTCGCCATTTTGCAACAATGTCGCAATTTCCTGTGGCGTCCCCTGGATTAGCTCGAg > 1:3434523/1‑69 (MQ=255)
aCCAATATCAGCTTCGCCATTTTGCAACAATGTCGCAATTTCCTGTGGCGTCCCCTGGATTAGCTCGAg > 1:2258749/1‑69 (MQ=255)
atatCAGCTTCGCCATTTTGCAACAATGTCGCAATTTCCTGTGGCGTCCCCTGGATTAGCTCGAGCCgt < 1:3346705/69‑2 (MQ=255)
atatCAGCTTCGCCATTTTGCAACAATGTCGCAATTTCCTGTGGCGTCCCCTGGATTAGCTCGAGCCGa < 1:3382019/69‑1 (MQ=255)
atatCAGCTTCGCCATTTTGCAACAATGTCGCAATTTCCTGTGGCGTCCCCTGGATTAGCTCGAGCCGa < 1:2216290/69‑1 (MQ=255)
atatCAGCTTCGCCATTTTGCAACAATGTCGCAATTTCCTGTGGCGTCCCCTGGATTAGCTCGAGCCGa < 1:1956732/69‑1 (MQ=255)
atatCAGCTTCGCCATTTTGCAACAATGTCGCAATTTCCTGTGGCGTCCCCTGGATTAGCTCGAGCCGa < 1:1881122/69‑1 (MQ=255)
atatCAGCTTCGCCATTTTGCAACAATGTCGCAATTTCCTGTGGCGTCCCCTGGATTAGCTCGAGCCGa < 1:1417802/69‑1 (MQ=255)
atatCAGCTTCGCCATTTTGCAACAATGTCGCAATTTCCTGTGGCGTCCCCTGGATTAGCTCGAGCCGa < 1:2461205/69‑1 (MQ=255)
cATTTTGCAACAATGTCGCAATTTCCTGTGGCGTCCCCTGGATTAGCTCGAGCCGAACCTCCGGGaaa < 1:577810/68‑1 (MQ=255)
aTTTTGCAACAATGTCGCAATTTCCTGTGGCGTCCCCTGGATTAGCTCGAGCCGAACCTCCGGGAAAAg < 1:2475816/69‑1 (MQ=255)
aTTTTGCAACAATGTCGCAATTTCCTGTGGCGTCCCCTGGATTAGCTCGAGCCGAACCTCCGGGAAAAg < 1:435476/69‑1 (MQ=255)
tttGCAACAACGTCGCAATTTCCTGTGGTGTACCCTGGATTAGCTCCAGCCGAACCTCCGGGa > 1:331489/1‑63 (MQ=255)
tttGCAACAACGTCGCAATTTCCTGTGGTGTACCCTGGATTAGCTCCAGCCGAACCTCCGGGa > 1:2411853/1‑63 (MQ=255)
tttGCAACAACGTCGCAATTTCCTGTGGTGTACCCTGGATTAGCTCCAGCCGAACCTCCGGGa > 1:868437/1‑63 (MQ=255)
tttGCAACAACGTCGCAATTTCCTGTGGTGTACCCTGGATTAGCTCCAGCCGAACCTCCGGGa > 1:3299173/1‑63 (MQ=255)
tttGCAACAACGTCGCAATTTCCTGTGGTGTACCCTGGATTAGCTCCAGCCGAACCTCCGGGa > 1:2226343/1‑63 (MQ=255)
tttGCAACAACGTCGCAATTTCCTGTGGTGTACCCTGGATTAGCTCCAGCCGAACCTCCGGGa > 1:2716171/1‑63 (MQ=255)
tttGCAACAACGTCGCAATTTCCTGTGGTGTACCCTGGATTAGCTCCAGCCGAACCTCCGGGa > 1:3243634/1‑63 (MQ=255)
tttGCAACAACGTCGCAATTTCCTGTGGTGTACCCTGGATTAGCTCCAGCCGAACCTCCGGGa > 1:320809/1‑63 (MQ=255)
tttGCAACAACGTCGCAATTTCCTGTGGTGTACCCTGGATTAGCTCCAGCCGAACCTCCGGGa > 1:3034404/1‑63 (MQ=255)
tttGCAACAACGTCGCAATTTCCTGTGGTGTACCCTGGATTAGCTCCAGCCGAACCTCCGGGa > 1:2190662/1‑63 (MQ=255)
tttGCAACAACGTCGCAATTTCCTGTGGTGTACCCTGGATTAGCTCCAGCCGAACCTCCGGGa > 1:1823508/1‑63 (MQ=255)
tttGCAACAACGTCGCAATTTCCTGTGGTGTACCCTGGATTAGCTCCAGCCGAACCTCCGGGa > 1:1762984/1‑63 (MQ=255)
tttGCAACAACGTCGCAATTTCCTGTGGTGTACCCTGGATTAGCTCCAGCCGAACCTCCGGGa > 1:738839/1‑63 (MQ=255)
tttGCAACAACGTCGCAATTTCCTGTGGTGTACCCTGGATTAGCTCCAGCCGAACCTCCGGGa > 1:1675227/1‑63 (MQ=255)
tttGCAACAACGTCGCAATTTCCTGTGGTGTACCCTGGATTAGCTCCAGCCGAACCTCCGGGa > 1:712178/1‑63 (MQ=255)
tttGCAACAACGTCGCAATTTCCTGTGGTGTACCCTGGATTAGCTCCAGCCGAACCTCCGGGa > 1:467437/1‑63 (MQ=255)
tGCAACAATGTCGCAATTTCCTGTGGCGTCCCCTGGATTAGCTCGAGCCGAACCTCCGGGAAAAg < 1:124799/65‑1 (MQ=255)
gCAATTTCCTGTGGCGTCCCCTGGATTAGCTCGAGCCGAACCTCCGGGAAAAGTTCGCGAAAAGCttt < 1:537777/68‑1 (MQ=255)
aaTTTCCTGTGGCGTCCCCTGGATTAGCTCGAGCCGAACCTCCGGGAAAAGTTCGCGAAAAGCTTTAAt < 1:2463215/69‑1 (MQ=255)
aaTTTCCTGTGGCGTCCCCTGGATTAGCTCGAGCCGAACCTCCGGGAAAAGTTCGCGAAAAGCTTTAAt < 1:620338/69‑1 (MQ=255)
tCCTGTGGCGTCCCCTGGATTAGCTCGAGCCGAACCTCCGGGAAAAGTTCGCGAAAAGCTTTAATGAcc > 1:1977045/1‑69 (MQ=255)
tCCTGTGGCGTCCCCTGGATTAGCTCGAGCCGAACCTCCGGGAAAAGTTCGCGAAAAGCTTTAATGAcc < 1:1042751/69‑1 (MQ=255)
tCCTGTGGCGTCCCCTGGATTAGCTCGAGCCGAACCTCCGGGAAAAGTTCGCGAAAAGCTTTAATGAcc > 1:1261594/1‑69 (MQ=255)
tCCTGTGGCGTCCCCTGGATTAGCTCGAGCCGAACCTCCGGGAAAAGTTCGCGAAAAGCTTTAATGAcc < 1:2677240/69‑1 (MQ=255)
tCCTGTGGCGTCCCCTGGATTAGCTCGAGCCGAACCTCCGGGAAAAGTTCGCGAAAAGCTTTAATGAcc > 1:14402/1‑69 (MQ=255)
tCCTGTGGCGTCCCCTGGATTAGCTCGAGCCGAACCTCCGGGAAAAGTTCGCGAAAAGCTTTAATGAcc > 1:1493766/1‑69 (MQ=255)
tCCTGTGGCGTCCCCTGGATTAGCTCGAGCCGAACCTCCGGGAAAAGTTCGCGAAAAGCTTTAATGAcc > 1:2432338/1‑69 (MQ=255)
tCCTGTGGCGTCCCCTGGATTAGCTCGAGCCGAACCTCCGGGAAAAGTTCGCGAAAAGCTTTAATGAcc > 1:1734634/1‑69 (MQ=255)
tCCTGTGGCGTCCCCTGGATTAGCTCGAGCCGAACCTCCGGGAAAAGTTCGCGAAAAGCTTTAATGAcc < 1:2302878/69‑1 (MQ=255)
tCCTGTGGCGTCCCCTGGATTAGCTCGAGCCGAACCTCCGGGAAAAGTTCGCGAAAAGCTTTAATGAcc < 1:353439/69‑1 (MQ=255)
tgtgGTGTACCCTTGATTAGCTCCAGCCGAACCTCCGGGAAAAGTTCGCGAAAAGCTTTATTGACctc < 1:1035155/68‑1 (MQ=255)
tgtgGTGTACCCTGGATTAGCTCCAGCCGAACCTCCGGGAAAAGTTCGCGAAAAGCTTTAATGACctc < 1:785902/68‑1 (MQ=255)
tgtgGTGTACCCTGGATTAGCTCCAGCCGAACCTCCGGGAAAAGTTCGCGAAAAGCTTTAATGACctc < 1:3270729/68‑1 (MQ=255)
tgtgGTGTACCCTGGATTAGCTCCAGCCGAACCTCCGGGAAAAGTTCGCGAAAAGCTTTAATGACctc < 1:1328644/68‑1 (MQ=255)
tgtgGTGTACCCTGGATTAGCTCCAGCCGAACCTCCGGGAAAAGTTCGCGAAAAGCTTTAATGACctc < 1:2436497/68‑1 (MQ=255)
tgtgGTGTACCCTGGATTAGCTCCAGCCGAACCTCCGGGAAAAGTTCGCGAAAAGCTTTAATGACctc < 1:1336575/68‑1 (MQ=255)
tgtgGTGTACCCTGGATTAGCTCCAGCCGAACCTCCGGGAAAAGTTCGCGAAAAGCTTTAATGACctc < 1:1387458/68‑1 (MQ=255)
tgtgGTGTACCCTGGATTAGCTCCAGCCGAACCTCCGGGAAAAGTTCGCGAAAAGCTTTAATGACctc < 1:478415/68‑1 (MQ=255)
tgtgGTGTACCCTGGATTAGCTCCAGCCGAAACTCCGGGAAAAGTTCGCTAAAAGCTTTAATGACctc < 1:1795364/68‑1 (MQ=255)
tgtgGTGTACCCTGGATTAGCTCCACCCGAACCTCCGGGAAAAGTTCGCGAAAAGCTTTAATGACctc < 1:2842121/68‑1 (MQ=255)
gATTAGCTCGAGCCGAACCTCCGGGAAAAGTTCGCGAAAAGCTTTAATGACCTCTGGCAAGCTATAACg > 1:2888766/1‑69 (MQ=255)
gATTAGCTCGAGCCGAACCTCCGGGAAAAGTTCGCGAAAAGCTTTAATGACCTCTGGCAAGCTATAACg > 1:663151/1‑69 (MQ=255)
gATTAGCTCGAGCCGAACCTCCGGGAAAAGTTCGCGAAAAGCTTTAATGACCTCTGGCAAGCTATAACg > 1:1657988/1‑69 (MQ=255)
cTCGAGCCGAACCTCCGGGAAAAGTTCGCGAAAAGCTTTAATGACCTCTGGCAAGCTATAACGTGCCt < 1:1031476/68‑1 (MQ=255)
cTCGAGCCGAACCTCCGGGAAAAGTTCGCGAAAAGCTTTAATGACCTCTGGCAAGCTATAACGTGCCt < 1:3361445/68‑1 (MQ=255)
cTCGAGCCGAACCTCCGGGAAAAGTTCGCGAAAAGCTTTAATGACCTCTGGCAAGCTATAACGTGCCt < 1:2659370/68‑1 (MQ=255)
cGAGCCGAACCTCCGGGAAAAGTTCGCGAAAAGCTTTAATGACCTCTGGCAAGCTATAACGTGCCTGAg < 1:1818005/69‑1 (MQ=255)
cGAGCCGAACCTCCGGGAAAAGTTCGCGAAAAGCTTTAATGACCTCTGGCAAGCTATAACGTGCCTGAg < 1:14584/69‑1 (MQ=255)
cGAGCCGAACCTCCGGGAAAAGTTCGCGAAAAGCTTTAATGACCTCTGGCAAGCTATAACGTGCCTGAg < 1:2006351/69‑1 (MQ=255)
cGAGCCGAACCTCCGGGAAAAGTTCGCGAAAAGCTTTAATGACCTCTGGCAAGCTATAACGTGCCTGAg < 1:2268265/69‑1 (MQ=255)
cGAGCCGAACCTCCGGGAAAAGTTCGCGAAAAGCTTTAATGACCTCTGGCAAGCTATAACGTGCCTGAg < 1:2519329/69‑1 (MQ=255)
cGAGCCGAACCTCCGGGAAAAGTTCGCGAAAAGCTTTAATGACCTCTGGCAAGCTATAACGTGCCTGAg < 1:629334/69‑1 (MQ=255)
cGAGCCGAACCTCCGGGAAAAGTTCGCGAAAAGCTTTAATGACCTCTGGCAAGCTATAACGTGCCTGAg < 1:638216/69‑1 (MQ=255)
cGAGCCGAACCTCCGGGAAAAGTTCGCGAAAAGCTTTAATGACCTCTGGCAAGCTATAACGTGCCTGAg < 1:1277168/69‑1 (MQ=255)
cGAGCCGAACCTCCGGGAAAAGTTCGCGAAAAGCTTTAATGACCTCTGGCAAGCTATAACGTGCCTGAg < 1:1272992/69‑1 (MQ=255)
cGAGCCGAACCTCCGGGAAAAGTTCGCGAAAAGCTTTAATGACCTCTGGCAAGCTATAACGTGCCTGAg < 1:735574/69‑1 (MQ=255)
cGAGCCGAACCTCCGGGAAAAGTTCGCGAAAAGCTTTAATGACCTCTGGCAAGCTATAACGTGCCTGAg < 1:1133479/69‑1 (MQ=255)
cGAGCCGAACCTCCGGGAAAAGTTCGCGAAAAGCTTTAATGACCTCTGGCAAGCTATAACGTGCCTGAg < 1:2841694/69‑1 (MQ=255)
cGAGCCGAACCTCCGGGAAAAGTTCGCGAAAAGCTTTAATGACCTCTGGCAAGCTATAACGTGCCTGAg < 1:857490/69‑1 (MQ=255)
gAGCCGAACCTCCGGGAAAAGTTCGCGAAAAGCTTTAATGACCTCTGGCAAGCTATAACGTGCCTGAg < 1:3024130/68‑1 (MQ=255)
|
ACCAATATCAGCTTCGCCATTTTGCAACAATGTCGCAATTTCCTGTGGCGTCCCCTGGATTAGCTCGAGCCGAACCTCCGGGAAAAGTTCGCGAAAAGCTTTAATGACCTCTGGCAAGCTATAACGTGCCTGAG > minE/1293788‑1293921
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
