Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,331,307 |
A→G |
68.2% |
I93I (ATT→ATC) |
cpsG ← |
phosphomannomutase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,331,307 | 0 | A | G | 68.2%
| 40.2
/ 20.3
| 47 | I93I (ATT→ATC) | cpsG | phosphomannomutase |
| Reads supporting (aligned to +/- strand): ref base A (5/10); new base G (14/18); total (19/28) |
| Fisher's exact test for biased strand distribution p-value = 5.42e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.36e-01 |
GAGCCCCCTCGCGAACCAGCTTCATGCCGTTATAATCCATCGGATTATGGCTGGCGGTAACTTCAATGCCGCCATCCACGCCGAGATGGAACGTGGCGAAATAGATCTCTTCGGTGCCGGACATACCAATA > minE/1331243‑1331373
|
gAGCCCCCTCGCGAACCAGCTTCATGCCGTTATAATCCATCGGATTATGGCTGGCGGTAACTTCAATgc < 1:1371157/69‑1 (MQ=255)
gAGCCCCCTCGCGAACCAGCTTCATGCCGTTATAATCCATCGGATTATGGCTGGCGGTAACTTCAATgc < 1:1396818/69‑1 (MQ=255)
gAGCCCCCTCGCGAACCAGCTTCATGCCGTTATAATCCATCGGATTATGGCTGGCGGTAACTTCAATgc < 1:499248/69‑1 (MQ=255)
gAGCCCCCTCGCGAACCAGCTTCATGCCGTTATAATCCATCGGATTATGGCTGGCGGTAACTTCAATgc < 1:3334682/69‑1 (MQ=255)
gAGCCCCCTCGCGAACCAGCTTCATGCCGTTATAATCCATCGGATTATGGCTGGCGGTAACTTCAATgc < 1:3005658/69‑1 (MQ=255)
gAGCCCCCTCGCGAACCAGCTTCATGCCGTTATAATCCATCGGATTATGGCTGGCGGTAACTTCAATgc < 1:2549971/69‑1 (MQ=255)
gcgcACCAGTTTCATGCCGTTGTAATCCATCGGGTTATGGCTGGCGGTAACTTCGATGCCGCCATCCAc > 1:982628/1‑69 (MQ=255)
gcgcACCAGTTTCATGCCGTTGTAATCCATCGGGTTATGGCTGGCGGTAACTTCGATGCCGCCATCCAc > 1:1222465/1‑69 (MQ=255)
gcgcACCAGTTTCATGCCGTTGTAATCCATCGGGTTATGGCTGGCGGTAACTTCGATGCCGCCATCCAc > 1:897224/1‑69 (MQ=255)
gcgcACCAGTTTCATGCCGTTGTAATCCATCGGGTTATGGCTGGCGGTAACTTCGATGCCGCCATCCAc > 1:805447/1‑69 (MQ=255)
gcgcACCAGTTTCATGCCGTTGTAATCCATCGGGTTATGGCTGGCGGTAACTTCGATGCCGCCATCCAc > 1:571594/1‑69 (MQ=255)
gcgcACCAGTTTCATGCCGTTGTAATCCATCGGGTTATGGCTGGCGGTAACTTCGATGCCGCCATCCAc > 1:2982465/1‑69 (MQ=255)
gcgcACCAGTTTCATGCCGTTGTAATCCATCGGGTTATGGCTGGCGGTAACTTCGATGCCGCCATCCAc > 1:2854249/1‑69 (MQ=255)
gcgcACCAGTTTCATGCCGTTGTAATCCATCGGGTTATGGCTGGCGGTAACTTCGATGCCGCCATCCAc > 1:2683491/1‑69 (MQ=255)
gcgcACCAGTTTCATGCCGTTGTAATCCATCGGGTTATGGCTGGCGGTAACTTCGATGCCGCCATCCAc > 1:2676858/1‑69 (MQ=255)
gcgcACCAGTTTCATGCCGTTGTAATCCATCGGGTTATGGCTGGCGGTAACTTCGATGCCGCCATCCAc > 1:2355276/1‑69 (MQ=255)
gcgcACCAGTTTCATGCCGTTGTAATCCATCGGGTTATGGCTGGCGGTAACTTCGATGCCGCCATCCAc > 1:1422855/1‑69 (MQ=255)
gcgcACCAGTTTCATGCCGTTGTAATCCATCGGGTTATGGCTGGCGGTAACTTCGATGCCGCCATCCAc > 1:2162857/1‑69 (MQ=255)
gcgcACCAGTTTCATGCCGTTGTAATCCATCGGGTTATGGCTGGCGGTAACTTCGATGCCGCCATCCAc > 1:1599911/1‑69 (MQ=255)
gcgcACCAGTTTCATGCCGTTGTAATCCATCGGGTTATGGCTGGCGGTAACTTCGATGCCGCCATCCAc > 1:1997813/1‑69 (MQ=255)
ccAGCTTCATGCCGTTATAATCCATCGGATTATGGCTGGCGGTAACTTCAATGCCGCCATCCACGCCga < 1:3267885/69‑1 (MQ=255)
ccAGCTTCATGCCGTTATAATCCATCGGATTATGGCTGGCGGTAACTTCAATGCCGCCATCCACGCCga < 1:3208687/69‑1 (MQ=255)
ccAGCTTCATGCCGTTATAATCCATCGGATTATGGCTGGCGGTAACTTCAATGCCGCCATCCACGCCga < 1:2889421/69‑1 (MQ=255)
ccAGCTTCATGCCGTTATAATCCATCGGATTATGGCTGGCGGTAACTTCAATGCCGCCATCCACGCCga < 1:2085940/69‑1 (MQ=255)
tATGGCTGGCGGTAACTTCGATGCCGCCATCCACGCCGAGATGTAACGTGGCGAAATAg < 1:3327543/59‑1 (MQ=255)
tATGGCTGGCGGTAACTTCGATGCCGCCATCCACGCCGAGATGGAACGTGGCGAAATAg < 1:495885/59‑1 (MQ=255)
tATGGCTGGCGGTAACTTCGATGCCGCCATCCACGCCGAGATGGAACGTGGCGAAATAg < 1:2466786/59‑1 (MQ=255)
tATGGCTGGCGGTAACTTCGATGCCGCCATCCACGCCGAGATGGAACGTGGCGAAATAg < 1:910727/59‑1 (MQ=255)
tATGGCTGGCGGTAACTTCGATGCCGCCATCCACGCCGAGATGGAACGTGGCGAAATAg < 1:1532704/59‑1 (MQ=255)
tATGGCTGGCGGTAACTTCGATGCCGCCATCCACGCCGAGATGGAACGTGGCGAAATAg < 1:874026/59‑1 (MQ=255)
tATGGCTGGCGGTAACTTCGATGCCGCCATCCACGCCGAGATGGAACGTGGCGAAATAg < 1:853066/59‑1 (MQ=255)
tATGGCTGGCGGTAACTTCGATGCCGCCATCCACGCCGAGATGGAACGTGGCGAAATAg < 1:1572441/59‑1 (MQ=255)
tATGGCTGGCGGTAACTTCGATGCCGCCATCCACGCCGAGATGGAACGTGGCGAAATAg < 1:733661/59‑1 (MQ=255)
tATGGCTGGCGGTAACTTCGATGCCGCCATCCACGCCGAGATGGAACGTGGCGAAATAg < 1:2675095/59‑1 (MQ=255)
tATGGCTGGCGGTAACTTCGATGCCGCCATCCACGCCGAGATGGAACGTGGCGAAATAg < 1:1613153/59‑1 (MQ=255)
tATGGCTGGCGGTAACTTCGATGCCGCCATCCACGCCGAGATGGAACGTGGCGAAATAg < 1:2834256/59‑1 (MQ=255)
tATGGCTGGCGGTAACTTCGATGCCGCCATCCACGCCGAGATGGAACGTGGCGAAATAg < 1:3420888/59‑1 (MQ=255)
tATGGCTGGCGGTAACTTCGATGCCGCCATCCACGCCGAGATGGAACGTGGCGAAATAg < 1:1631283/59‑1 (MQ=255)
tATGGCTGGCGGTAACTTCGATGCCGCCATCCACGCCGAGATGGAACGTGGCGAAATAg < 1:1685960/59‑1 (MQ=255)
tATGGCTGGCGGTAACTTCGATGCCGCCATCCACGCCGAGATGGAACGTGGCGAAATAg < 1:1692993/59‑1 (MQ=255)
tATGGCTGGCGGTAACTTCGATGCCGCCATCCACGCCGAGATGGAACGTGGCGAAATAg < 1:2700651/59‑1 (MQ=255)
tATGGCTGGCGGTAACTTCGATGCCGCCATCCACGCCGAGATGGAACGTGGCGAAATAg < 1:1884593/59‑1 (MQ=255)
aaCTTCAATGCCGCCATCCACGCCGAGATGGAACGTGGCGAAATAGATCTCTTCGGTGCCGGACATAcc > 1:3056450/1‑69 (MQ=255)
tCAATGCCGCCATCCACGCCGAGATGGAACGTGGCGAAATAGATCTCTTCGGTGCCGGACATACCAata > 1:2164512/1‑69 (MQ=255)
tCAATGCCGCCATCCACGCCGAGATGGAACGTGGCGAAATAGATCTCTTCGGTGCCGGACATACCAata > 1:825232/1‑69 (MQ=255)
tCAATGCCGCCATCCACGCCGAGATGGAACGTGGCGAAATAGATCTCTTCGGTGCCGGACATACCAata > 1:3036113/1‑69 (MQ=255)
tCAATGCCGCCATCCACGCCGAGATGGAACGTGGCGAAATAGATCTCTTCGGTGCCGGACATACCAata > 1:975302/1‑69 (MQ=255)
|
GAGCCCCCTCGCGAACCAGCTTCATGCCGTTATAATCCATCGGATTATGGCTGGCGGTAACTTCAATGCCGCCATCCACGCCGAGATGGAACGTGGCGAAATAGATCTCTTCGGTGCCGGACATACCAATA > minE/1331243‑1331373
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
