Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,359,734 |
G→A |
45.1% |
R41R (CGC→CGT) |
yehC ← |
predicted periplasmic pilin chaperone |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,359,734 | 0 | G | A | 45.1%
| 17.5
/ 64.1
| 82 | R41R (CGC→CGT) | yehC | predicted periplasmic pilin chaperone |
| Reads supporting (aligned to +/- strand): ref base G (18/27); new base A (19/18); total (37/45) |
| Fisher's exact test for biased strand distribution p-value = 3.74e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01 |
AAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAACAATTCCGGCATGTGCAGGAAGGACCATAGAAAAAATGAGTA > minE/1359670‑1359792
|
aagaaCGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTGTGCTCTGCCGGGTAAATAATAcgcg < 1:3288472/69‑1 (MQ=255)
aagaaCGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTGTGCTCTGCCGGGTAAATAATAcgcg < 1:2122658/69‑1 (MQ=255)
aagaaCGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTGTGCTCTGCCGGGTAAATAATAcgcg < 1:2898996/69‑1 (MQ=255)
aagaaCGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTGTGCTCTGCCGGGTAAATAATAcgcg < 1:127003/69‑1 (MQ=255)
aagaaCGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTGTGCTCTGCCGGGTAAATAATAcgcg < 1:131029/69‑1 (MQ=255)
aagaaCGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTGTGCTCTGCCGGGTAAATAATAcgcg < 1:578916/69‑1 (MQ=255)
aagaaCGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTGTGCTCTGCCGGGTAAATAATAcgcg < 1:135001/69‑1 (MQ=255)
aagaaCGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTGTGCTCTGCCGGGTAAATAATAcgcg < 1:3092483/69‑1 (MQ=255)
aagaaCGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTGTGCTCTGCCGGGTAAATAATAcgcg < 1:2655429/69‑1 (MQ=255)
aagaaCGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTGTGCTCTGCCGGGTAAATAATAcgcg < 1:332925/69‑1 (MQ=255)
aagaaCGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTGTGCTCTGCCGGGTAAATAATAcgcg < 1:548162/69‑1 (MQ=255)
aagaaCGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTGTGCTCTGCCGGGTAAATAATAcgcg < 1:1924127/69‑1 (MQ=255)
aagaaCGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTGTGCTCTGCCGGGTAAATAATAcgcg < 1:911756/69‑1 (MQ=255)
agaaCGGTTTGCCTGGTTCATCAACTGCACCATCACTTCTTTGTGCTCTGCCGGGTAAATAATAcgcg < 1:1392413/68‑1 (MQ=255)
agaaCGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTGTGCTCTGCCGGGTAATTAATAcgcg < 1:1989209/68‑1 (MQ=255)
agaaCGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTGTGCTCTGCCGGGTAAATAATAcgcg < 1:775827/68‑1 (MQ=255)
agaaCGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTGTGCTCTGCCGGGTAAATAATAcgcg < 1:536266/68‑1 (MQ=255)
agaaCGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTGTGCTCTGCCGGGTAAATAATAcgcg < 1:889278/68‑1 (MQ=255)
gTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTa < 1:2517420/69‑1 (MQ=255)
gTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTa < 1:1431142/69‑1 (MQ=255)
gTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTa < 1:2096407/69‑1 (MQ=255)
gTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTa < 1:287325/69‑1 (MQ=255)
gTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTa < 1:1099209/69‑1 (MQ=255)
tGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAAc < 1:887343/68‑1 (MQ=255)
tGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAAc < 1:2970110/68‑1 (MQ=255)
tGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAAc < 1:195591/68‑1 (MQ=255)
tGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAAc < 1:1616914/68‑1 (MQ=255)
tGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAAc < 1:595272/68‑1 (MQ=255)
tcatcaACTGCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAAc > 1:452147/1‑64 (MQ=255)
tcatcaACTGCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAAc > 1:741699/1‑64 (MQ=255)
caACTGCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAACAATTCCgg < 1:2416402/68‑1 (MQ=255)
caACTGCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAACAATTCCgg < 1:1375628/68‑1 (MQ=255)
aaCTGCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAACAATTCCGGCa > 1:3127385/1‑69 (MQ=255)
aaCTGCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAACAATTCCGGCa > 1:549915/1‑69 (MQ=255)
aaCTGCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAACAATTCCGGCa > 1:2902060/1‑69 (MQ=255)
aaCTGCACAATCACTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAACAATTCCGGc > 1:2518633/1‑68 (MQ=255)
aaCTGCA‑CATCACTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCTTa < 1:3108028/52‑1 (MQ=255)
gCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAACAATTCCGGCAtg > 1:3250080/1‑67 (MQ=255)
gCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAACAATTCCGGCAtg > 1:678259/1‑67 (MQ=255)
gCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAACAATTCCGGCAtg > 1:3345353/1‑67 (MQ=255)
gCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAACAATTCCGGCAtg > 1:2841305/1‑67 (MQ=255)
gCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAACAATTCCGGCAtg > 1:1451263/1‑67 (MQ=255)
gCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAACAATTCCGGCAtg > 1:2772161/1‑67 (MQ=255)
gCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAACAATTCCGGCAtg > 1:2419513/1‑67 (MQ=255)
cACTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAACAATTCCGGCATGTGCaggaag < 1:3080128/69‑1 (MQ=255)
aCTTCTTTGTGCTCTGCCGGGTAAATAATACGCGTCCCGTAGATAACaa > 1:2665076/1‑49 (MQ=37)
aCTTCTTTGTGCTCTGCCGGGTAAATAATACGCGTCCCGTAGATAACAATAcc > 1:2802822/1‑53 (MQ=255)
aCTTCTTTGTGCTCTGCCGGGTAAATAATACGCGTCCCGTAGATAACAATAcc > 1:986446/1‑53 (MQ=255)
aCTTCTTTGTGCTCTGCCGGGTAAATAATACGCGTCCCGTAGATAACAATAcc > 1:1785451/1‑53 (MQ=255)
aCTTCTTTGTGCTCTGCCGGGTAAATAATACGCGTCCCGTAGATAACAATAcc > 1:1822103/1‑53 (MQ=255)
aCTTCTTTGTGCTCTGCCGGGTAAATAATACGCGTCCCGTAGATAACAATAcc > 1:436999/1‑53 (MQ=255)
aCTTCTTTGTGCTCTGCCGGGTAAATAATACGCGTCCCGTAGATAACAATAcc > 1:35341/1‑53 (MQ=255)
aCTTCTTTGTGCTCTGCCGGGTAAATAATACGCGTCCCGTAGATAACAATAcc > 1:191225/1‑53 (MQ=255)
aCTTCTTTGTGCTCTGCCGGGTAAATAATACGCGTCCCGTAGATAACAATAcc > 1:1948694/1‑53 (MQ=255)
aCTTCTTTGTGCTCTGCCGGGTAAATAATACGCGTCCCGTAGATAACAATAcc > 1:2187782/1‑53 (MQ=255)
aCTTCTTTGTGCTCTGCCGGGTAAATAATACGCGTCCCGTAGATAACAATAcc > 1:2237236/1‑53 (MQ=255)
aCTTCTTTGTGCTCTGCCGGGTAAATAATACGCGTCCCGTAGATAACAATAcc > 1:2242891/1‑53 (MQ=255)
aCTTCTTTGTGCTCTGCCGGGTAAATAATACGCGTCCCGTAGATAACAATAcc > 1:2272617/1‑53 (MQ=255)
aCTTCTTTGTGCTCTGCCGGGTAAATAATACGCGTCCCGTAGATAACAATAcc > 1:2366736/1‑53 (MQ=255)
aCTTCTTTGTGCTCTGCCGGGTAAATAATACGCGTCCCGTAGATAACAATAcc > 1:2383504/1‑53 (MQ=255)
aCTTCTTTGTGCTCTGCCGGGTAAATAATACGCGTCCCGTAGATAACAATAcc > 1:2709908/1‑53 (MQ=255)
aCTTCTTTGTGCTCTGCCGGGTAAATAATACGCGTCCCGTAGATAACAATAcc > 1:2778086/1‑53 (MQ=255)
aCTTCTTTGTGCTCTGCCGGGTAAATAATACGCGTCCCGTAGATAACAATAcc > 1:2865533/1‑53 (MQ=255)
aCTTCTTTGTGCTCTGCCGGGTAAATAATACGCGTCCCGTAGATAACAATAcc > 1:2803284/1‑53 (MQ=255)
aCTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTa < 1:91890/41‑1 (MQ=255)
aCTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAACAATTCCGGCATGTGCaggaagg > 1:2924633/1‑69 (MQ=255)
aCTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAACAATTCCGGCATGTGCaggaagg > 1:285979/1‑69 (MQ=255)
aCTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAACAATTCCGGCATGTGCaggaagg > 1:1342583/1‑69 (MQ=255)
aCTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAACAATTCCGGCATGTGCaggaagg > 1:1235336/1‑69 (MQ=255)
ttcttTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAACAATTCCGGCATGTGCAGGAAGGAc < 1:75621/69‑1 (MQ=255)
ttcttTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAACAATTCCGGCATGTGCAGGAAGGAc < 1:711363/69‑1 (MQ=255)
ttcttTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAACAATTCCGGCATGTGCAGGAAGGAc < 1:1107913/69‑1 (MQ=255)
ttcttTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAACAATTCCGGCATGTGCAGGAAGGAc < 1:3354443/69‑1 (MQ=255)
ttcttTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAACAATTCCGGCATGTGCAGGAAGGAc < 1:3220594/69‑1 (MQ=255)
ttcttTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAACAATTCCGGCATGTGCAGGAAGGAc < 1:1851866/69‑1 (MQ=255)
ttcttTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAACAATTCCGGCATGTGCAGGAAGGAc < 1:1675215/69‑1 (MQ=255)
cttTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAACAATTCCGGCATGTGCAGGAAGGAc > 1:659065/1‑67 (MQ=255)
ttCTGCCGGGTAAATAATGCGCGTCCCGTAGATAACAATTCCGGCATGTGCAGGAAGGACCATAGaaaa < 1:2975909/69‑1 (MQ=255)
ttCTGCCGGGTAAATAATGCGCGTCCCGTAGATAACAATTCCGGCATGTGCAGGAAGGACCATAGaaaa < 1:1213525/69‑1 (MQ=255)
ttCTGCCGGGTAAATAATGCGCGTCCCGTAGATAACAATTCCGGCATGTGCAGGAAGGACCATAGaaaa < 1:996539/69‑1 (MQ=255)
tCTGCCGGGTAAATAATGCGCGTCCCGTAGATAACAATTCCGGCATGTGCACGAAGGACCATAGaaaa < 1:665808/68‑1 (MQ=255)
ggTAAATAATGCGCGTCCCGTAGATAACAATTCCGGCATGTGCAGGAAGGACCATAGAAAAAATGAGTa < 1:2626808/69‑1 (MQ=255)
|
AAGAACGGTTTCCCTGGTTCATCAACTGCACCATCACTTCTTTATTTTCTGCCGGGTAAATAATGCGCGTCCCGTAGATAACAATTCCGGCATGTGCAGGAAGGACCATAGAAAAAATGAGTA > minE/1359670‑1359792
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
