Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,398,612 |
C→G |
42.4% |
A312A (GCC→GCG) |
yeiQ → |
predicted dehydrogenase, NAD‑dependent |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,398,612 | 0 | C | G | 42.4%
| 36.5
/ 89.1
| 90 | A312A (GCC→GCG) | yeiQ | predicted dehydrogenase, NAD‑dependent |
| Reads supporting (aligned to +/- strand): ref base C (31/20); new base G (19/19); total (51/39) |
| Fisher's exact test for biased strand distribution p-value = 3.89e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
ATGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATTGCATGCAGGATCGCGCATTTCGCCATGCCGCCAGAACATTAATGCTGGATGAGCA > minE/1398544‑1398680
|
aTGAAACTGCGGATGCTTAATGGCAGCCTCTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGcc < 1:135879/69‑1 (MQ=255)
aTGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGcc < 1:1238072/69‑1 (MQ=255)
aTGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGcc < 1:834640/69‑1 (MQ=255)
aTGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGcc < 1:1398201/69‑1 (MQ=255)
aTGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGcc < 1:629268/69‑1 (MQ=255)
tGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGcc < 1:1118788/68‑1 (MQ=255)
tGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGcc > 1:3249637/1‑68 (MQ=255)
tGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGcc > 1:3121170/1‑68 (MQ=255)
gCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCat < 1:1784798/64‑1 (MQ=255)
gCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCat < 1:1997843/64‑1 (MQ=255)
gCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCat < 1:2019758/64‑1 (MQ=255)
gCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCat < 1:2218798/64‑1 (MQ=255)
gCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCat < 1:2505649/64‑1 (MQ=255)
gCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCat < 1:333630/64‑1 (MQ=255)
gCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCat < 1:113331/64‑1 (MQ=255)
cGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAg > 1:1832779/1‑68 (MQ=255)
cGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGt > 1:2100629/1‑69 (MQ=255)
cGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGt > 1:998017/1‑69 (MQ=255)
gCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATtg > 1:2759817/1‑69 (MQ=255)
gCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATtg > 1:1875120/1‑69 (MQ=255)
cTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATtgc > 1:1180177/1‑69 (MQ=255)
cTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATtgc > 1:1809544/1‑69 (MQ=255)
ggCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATTGCATGCAg > 1:1491423/1‑69 (MQ=255)
gCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATTGCATGCAgg > 1:2344654/1‑69 (MQ=255)
aCTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCGCACATCAGTGATTGCATGCAGGa < 1:958098/64‑1 (MQ=255)
aCTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCGCACATCAGTGATTGCATGCAGGa < 1:2832070/64‑1 (MQ=255)
aCTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCGCACATCAGTGATTGCATGCAGGa < 1:2402129/64‑1 (MQ=255)
aCTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCGCACATCAGTGATTGCATGCAGGa < 1:2236414/64‑1 (MQ=255)
aCTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCGCACATCAGTGATTGCATGCAGGa < 1:2151073/64‑1 (MQ=255)
aCTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCGCACATCAGTGATTGCATGCAGGa < 1:1867407/64‑1 (MQ=255)
aCTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATTGCATGCAGGATcgcg > 1:1946379/1‑69 (MQ=255)
aCTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATTGCATGCAGGATcgcg > 1:3125898/1‑69 (MQ=255)
aCTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATTGCATGCAGGATcgcg > 1:1442945/1‑69 (MQ=255)
aCTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATTGCATGCAGGATcgcg > 1:370002/1‑69 (MQ=255)
aCTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATTGCATGCAGGATcgcg > 1:2098273/1‑69 (MQ=255)
tctTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATTGCATGCAGGATCGCGCa < 1:594646/69‑1 (MQ=255)
tctTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATTGCATGCAGGATCGCGCa < 1:1455869/69‑1 (MQ=255)
tctTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATTGCATGCAGGATCGCGCa < 1:2186087/69‑1 (MQ=255)
tctTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATTGCATGCAGGATCGCGCa < 1:1729467/69‑1 (MQ=255)
tctTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATTGCATGCAGGATCGCGCa < 1:489452/69‑1 (MQ=255)
ctTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATTGCATGCAgg > 1:2084599/1‑60 (MQ=255)
ctTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATTGCATGCAgg > 1:544732/1‑60 (MQ=255)
ctTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATTGCATGCAGGATCGCGCa > 1:2085428/1‑68 (MQ=255)
ctTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATTGCATGCAGGATCGCGCa > 1:1523435/1‑68 (MQ=255)
ctTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATTGCATGCAGGATCGCGCa > 1:420853/1‑68 (MQ=255)
ctTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATTGCATGCAGGATCGCGCAt > 1:3080506/1‑69 (MQ=255)
ctTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATTGCATGCAGGATCGCGCAt > 1:3125950/1‑69 (MQ=255)
ctTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATTGCATGCAGGATCGCGCAt > 1:2378833/1‑69 (MQ=255)
ctTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATTGCATGCAGGATCGCGCAt > 1:3431721/1‑69 (MQ=255)
ctTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCAAATATAAGTGATTTCATGCAGGAt > 1:2519385/1‑62 (MQ=255)
ttttCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATtg < 1:853879/51‑1 (MQ=255)
ttttCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATtg < 1:2416575/51‑1 (MQ=255)
ttttCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATtg < 1:455417/51‑1 (MQ=255)
ctcGCTTATCTGGATTACCTCTCAGGATTCGCCCATATCAGTGATTGCATGCAGGATCGCGCATTTCGc > 1:1644078/1‑69 (MQ=255)
gCTTATCTGGGTTACCTCTCAGGATTCGCCCGTATCAGTGATTGCATGCAGGATCGCGCATTTCGCCAt > 1:2576403/1‑69 (MQ=255)
gCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATtgcatgc > 1:3131470/1‑49 (MQ=255)
gCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATTGCATGCAGGATCGCGCATTTCGCCAt > 1:1509649/1‑69 (MQ=255)
gCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATTGCATGCAGGATCGCGCATTTCGCCAt > 1:250641/1‑69 (MQ=255)
gCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATTGCATGCAGGATCGCGCATTTCGCCAt > 1:3043623/1‑69 (MQ=255)
tATCTGGGTTACCTCTCAGGATTCGCGCACATCAGTGATTGCATGCAGGATCGCGCATTTc > 1:1384293/1‑61 (MQ=255)
tATCTGGGTTACCTCTCAGGATTCGCGCACATCAGTGATTGCATGCAGGATCGCGCATTTc > 1:717871/1‑61 (MQ=255)
tATCTGGGTTACCTCTCAGGATTCGCGCACATCAGTGATTGCATGCAGGATCGCGCATTTc > 1:455282/1‑61 (MQ=255)
tATCTGGGTTACCTCTCAGGATTCGCGCACATCAGTGATTGCATGCAGGATCGCGCATTTc > 1:473066/1‑61 (MQ=255)
tATCTGGGTTACCTCTCAGGATTCGCGCACATCAGTGATTGCATGCAGGATCGCGCATTTc > 1:1759068/1‑61 (MQ=255)
tATCTGGGTTACCTCTCAGGATTCGCGCACATCAGTGATTGCATGCAGGATCGCGCATTTc > 1:1428243/1‑61 (MQ=255)
tATCTGGGTTACCTCTCAGGATTCGCGCACATCAGTGATTGCATGCAGGATCGCGCATTTc > 1:3124963/1‑61 (MQ=255)
tATCTGGGTTACCTCTCAGGATTCGCGCACATCAGTGATTGCATGCAGGATCGCGCATTTc > 1:543894/1‑61 (MQ=255)
tATCTGGGTTACCTCTCAGGATTCGCGCACATCAGTGATTGCATGCAGGATCGCGCATTTc > 1:157781/1‑61 (MQ=255)
tATCTGGGTTACCTCTCAGGATTCGCGCACATCAGTGATTGCATGCAGGATCGCGCATTTc > 1:2036263/1‑61 (MQ=255)
tATCTGGGTTACCTCTCAGGATTCGCGCACATCAGTGATTGCATGCAGGATCGCGCATTTc > 1:1832000/1‑61 (MQ=255)
tATCTGGGTTACCTCTCAGGATTCGCGCACATCAGTGATTGCATGCAGGATCGCGCATTTc > 1:2795564/1‑61 (MQ=255)
tATCTGGGTTACCTCTCAGGATTCGCGCACATCAGTGATTGCATGCAGGATCGCGCATTTc > 1:2793598/1‑61 (MQ=255)
tATCTGGGTTACCTCTCAGGATTCGCGCACATCAGTGATTGCATGCAGGATCGCGCATTTc > 1:2776687/1‑61 (MQ=255)
tATCTGGGTTACCTCTCAGGATTCGCGCACATCAGTGATTGCATGCAGGATCGCGCATTTc > 1:1506074/1‑61 (MQ=255)
tATCTGGGTTACCTCTCAGGATTCGCGCACATCAGTGATTGCATGCAGGATCGCGCATTTc > 1:2202564/1‑61 (MQ=255)
tATCTGGGTTACCTCTCAGGATTCGCGCACATCAGTGATTGCATGCAGGATCGCGCATTTc > 1:1504768/1‑61 (MQ=255)
tATCTGGGTTACCTCTCAGGATTCGCGCACATCAGTGATTGCATGCAGGATCGCGCATTTc > 1:2362178/1‑61 (MQ=255)
tATCTGGGTTACCTCTCAGGATTCGAGCACATCAGTGATTGCATGCAGGATCGCGCATTTc > 1:422101/1‑61 (MQ=255)
ctctCAGGATTCGCCCATATCGGTGATTGCATGCAGGATCGCGCATTTCGCCATGCCGCCATAACAtt < 1:564195/68‑1 (MQ=255)
ctctCAGGATTCGCCCATATCAGTGATTGCATGCAGGATCGCGCATTTCGCCATGCCGCCAGAACAtt < 1:779559/68‑1 (MQ=255)
ctctCAGGATTCGCCCATATCAGTGATTGCATGCAGGATCGCGCATTTCGCCATGCCGCCAGAACAtt < 1:1572922/68‑1 (MQ=255)
ctctCAGGATTCGCCCATATCAGTGATTGCATGCAGGATCGCGCATTTCGCCATGCCGCCAGAACAtt < 1:2961133/68‑1 (MQ=255)
ctctCAGGATTCGCCCATATCAGTGATTGCATGCAGGATCGCGCATTTCGCCATGCCGCCAGAACAtt < 1:3024160/68‑1 (MQ=255)
ctcAGGATTCGCGCACATCAGTGATTGCATGCAGGATCGCGCATTTCGCCATGCCGCCAGa < 1:1859470/61‑1 (MQ=255)
ctcAGGATTCGCGCACATCAGTGATTGCATGCAGGATCGCGCATTTCGCCATGCCGCCAGa < 1:688266/61‑1 (MQ=255)
ctcAGGATTCGCGCACATCAGTGATTGCATGCAGGATCGCGCATTTCGCCATGCCGCCAGa < 1:1643792/61‑1 (MQ=255)
ctcAGGATTCGCGCACATCAGTGATTGCATGCAGGATCGCGCATTTCGCCATGCCGCCAGa < 1:82093/61‑1 (MQ=255)
ctcAGGATTCGCGCACATCAGTGATTGCATGCAGGATCGCGCATTTCGCCATGCCGCCAGa < 1:604331/61‑1 (MQ=255)
ctcAGGATTCGCGCACATCAGTGATTGCATGCAGGATCGCGCATTTCGCCATGCCGCCAGa < 1:2021892/61‑1 (MQ=255)
ctcAGGATTCGCGCACATCAGTGATTGCATGCAGGATCGCGCATTTCGCCATGCCGCCAGa < 1:2164674/61‑1 (MQ=255)
ctcAGGATTCGCGCACATCAGTGATTGCATGCAGGATCGCGCATTTCGCCATGCCGCCAGa < 1:2335380/61‑1 (MQ=255)
ctcAGGATTCGCGCACATCAGTGATTGCATGCAGGATCGCGCATTTCGCCATGCCGCCAGa < 1:2366060/61‑1 (MQ=255)
ctcAGGATTCGCGCACATCAGTGATTGCATGCAGGATCGCGCATTTCGCCATGCCGCCAGa < 1:3408188/61‑1 (MQ=255)
ctcAGGATTCGCGCACATCAGTGATTGCATGCAGGATCGCGCATTTCGCCATGCCGCCAGa < 1:2745621/61‑1 (MQ=255)
ctcAGGATTCGCGCACATCAGTGATTGCATGCAGGATCGCGCATTTCGCCATGCCGCCAGa < 1:2863524/61‑1 (MQ=255)
ctcAGGATTCGCGCACATCAGTGATTGCATGCAGGATCGCGCATTTCGCCATGCCGCCAGa < 1:1461994/61‑1 (MQ=255)
aGGATTCGCCCATATCAGTGATTGCATGCAGGATCGCGCATTTCGCCATGCCGCCAGAACATTAATGc > 1:2454382/1‑68 (MQ=255)
tCGCCCATATCAGTGATTGCATGCAGGATCGCGCATTTCGCCATGCCGCCAGAACATTAATGCTGGATg > 1:1911713/1‑69 (MQ=255)
ccATATCAGTGATTGCATGCAGGATCGCGCATTTCGCCATGCCGCCAGAACATTAATGCTGGATGAGCa < 1:3005471/69‑1 (MQ=255)
|
ATGAAACTGCGGATGCTTAATGGCAGCCACTCTTTTCTCGCTTATCTGGGTTACCTCTCAGGATTCGCCCATATCAGTGATTGCATGCAGGATCGCGCATTTCGCCATGCCGCCAGAACATTAATGCTGGATGAGCA > minE/1398544‑1398680
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
