Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,408,517 |
A→T |
51.7% |
S262S (TCA→TCT) |
yejF → |
fused predicted oligopeptide transporter subunits and ATP‑binding components of ABC superfamily |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,408,517 | 0 | A | T | 51.7%
| ‑0.2
/ 69.2
| 58 | S262S (TCA→TCT) | yejF | fused predicted oligopeptide transporter subunits and ATP‑binding components of ABC superfamily |
| Reads supporting (aligned to +/- strand): ref base A (9/19); new base T (14/16); total (23/35) |
| Fisher's exact test for biased strand distribution p-value = 2.94e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TACGCTATTTGCATCACCCACTCATCCTTACACACAAAAGCTACTCAACAGTGAACCGTCAGGCGATCCAGTGCCGTTGCCAGAACCTGCCTCAACGTTGCTGGATGTTGAACAGCTTCAG > minE/1408457‑1408577
|
tacgctaTTTGCATCACCCACTCATCCTTACACACAAAATCTACTCAACAGTGAACCGTCAGGCGATcc < 1:3109436/69‑1 (MQ=255)
tacgctaTTTGCATCACCCACTCATCCTTACACACAAAAGCTACTCAACAGTGAACCGTCAGGCGATcc < 1:805437/69‑1 (MQ=255)
acgctaTTTGCATCACCCACTCATCCTTACACACAAAAGCTACTCAACAGTGAACCTTCAGGCGATcc < 1:297250/68‑1 (MQ=255)
acgctaTTTGCATCACCCACTCATCCTTACACACAAAAGCTACTCAACAGTGAACCGTCAGGCGATCCa > 1:2437753/1‑69 (MQ=255)
cACCCACTCATCCTTACACACAAAAGCTACTCAACAGTGAACCGTCAGGCGATCCAGTGCCGTTGCCAg < 1:3286970/69‑1 (MQ=255)
ccACTCATCCTTACACACAAAAGCTACTCAACAGTGAACCGTCAGGCGATCCAGTGCCGTTGCCAGaa < 1:805914/68‑1 (MQ=255)
ccACTCATCCTTACACACAAAAGCTACTCAACAGTGAACCGTCAGGCGATCCAGTGCCGTTGCCAGaa < 1:749067/68‑1 (MQ=255)
cTTACACACAAAAGCTACTCAACAGTGAACCGTCAGGCGATCCAGTGCCGTTGCCAGAACCTGCCTCaa > 1:170363/1‑69 (MQ=255)
cTTACACACAAAAGCTACTCAACAGTGAACCGTCAGGCGATCCAGTGCCGTTGCCAGAACCTGCCTCaa > 1:1769735/1‑69 (MQ=255)
cTTACACACAAAAGCTACTCAACAGTGAACCGTCAGGCGATCCAGTGCCGTTGCCAGAACCTGCCTCaa > 1:686253/1‑69 (MQ=255)
cTTACACACAAAAGCTACTCAACAGTGAACCGTCAGGCGATCCAGTGCCGTTGCCAGAACCTGCCTCaa > 1:3338646/1‑69 (MQ=255)
cTTACACACAAAAGCTACTCAACAGTGAACCGTCAGGCGATCCAGTGCCGTTGCCAGAACCTGCCTCaa > 1:682365/1‑69 (MQ=255)
cTTACACACAAAAGCTACTCAACAGTGAACCGTCAGGCGATCCAGTGCCGTTGCCAGAACCTGCCTCaa > 1:2867504/1‑69 (MQ=255)
tACTCAACAGTGAACCGTCAGGCGATCCAGTGCCGTTGCCAGAACCTGCCTTAACGTTGCTGGATGTTg < 1:3023576/69‑1 (MQ=255)
tACTCAACAGTGAACCGTCAGGCGATCCAGTGCCGTTGCCAGAACCTGCCTCAACGTTGCTGGATGTTg < 1:3207870/69‑1 (MQ=255)
tACTCAACAGTGAACCGTCAGGCGATCCAGTGCCGTTGCCAGAACCTGCCTCAACGTTGCTGGATGTTg < 1:2933077/69‑1 (MQ=255)
tACTCAACAGTGAACCGTCAGGCGATCCAGTGCCGTTGCCAGAACCTGCCTCAACGTTGCTGGATGTTg < 1:3130351/69‑1 (MQ=255)
tACTCAACAGTGAACCGTCAGGCGATCCAGTGCCGTTGCCAGAACCTGCCTCAACGTTGCTGGATGTTg < 1:2791023/69‑1 (MQ=255)
tACTCAACAGTGAACCGTCAGGCGATCCAGTGCCGTTGCCAGAACCTGCCTCAACGTTGCTGGATGTTg < 1:519928/69‑1 (MQ=255)
tACTCAACAGTGAACCGTCAGGCGATCCAGTGCCGTTGCCAGAACCTGCCTCAACGTTGCTGGATGTTg < 1:2110158/69‑1 (MQ=255)
tACTCAACAGTGAACCGTCAGGCGATCCAGTGCCGTTGCCAGAACCTGCCTCAACGTTGCTGGATGTTg < 1:1893609/69‑1 (MQ=255)
tACTCAACAGTGAACCGTCAGGCGATCCAGTGCCGTTGCCAGAACCTGCCTCAACGTTGCTGGATGTTg < 1:1739016/69‑1 (MQ=255)
tACTCAACAGTGAACCGTCAGGCGATCCAGTGCCGTTGCCAGAACCTGCCTCAACGTTGCTGGATGTTg < 1:1720089/69‑1 (MQ=255)
tACTCAACAGTGAACCGTCAGGCGATCCAGTGCCGTTGCCAGAACCTGCCTCAACGTTGCTGGATGTTg < 1:152035/69‑1 (MQ=255)
tACTCAACAGTGAACCGTCAGGCGATCCAGTGCCGTTGCCAGAACCTGCCTCAACGTTGCTGGATGTTg < 1:1308846/69‑1 (MQ=255)
tACTCAACAGTGAACCGTCAGGCGATCCAGTGCCGTTGCCAGAACCTGCCTCAACGTTGCTGGATGTTg < 1:1249627/69‑1 (MQ=255)
aCTCAACAGTGAACCATCTGGCGACCCGGTGCCATTGCCAGAACCCGCCTCAACGTTGCTAGATGTTGa < 1:395955/69‑1 (MQ=255)
aCTCAACAGTGAACCATCTGGCGACCCGGTGCCATTGCCAGAACCCGCCTCAACGTTGCTAGATGTTGa < 1:3405314/69‑1 (MQ=255)
aCTCAACAGTGAACCATCTGGCGACCCGGTGCCATTGCCAGAACCCGCCTCAACGTTGCTAGATGTTGa < 1:335550/69‑1 (MQ=255)
aCTCAACAGTGAACCATCTGGCGACCCGGTGCCATTGCCAGAACCCGCCTCAACGTTGCTAGATGTTGa < 1:3350410/69‑1 (MQ=255)
aCTCAACAGTGAACCATCTGGCGACCCGGTGCCATTGCCAGAACCCGCCTCAACGTTGCTAGATGTTGa < 1:460882/69‑1 (MQ=255)
aCTCAACAGTGAACCATCTGGCGACCCGGTGCCATTGCCAGAACCCGCCTCAACGTTGCTAGATGTTGa < 1:3348959/69‑1 (MQ=255)
aCTCAACAGTGAACCATCTGGCGACCCGGTGCCATTGCCAGAACCCGCCTCAACGTTGCTAGATGTTGa < 1:3293252/69‑1 (MQ=255)
aCTCAACAGTGAACCATCTGGCGACCCGGTGCCATTGCCAGAACCCGCCTCAACGTTGCTAGATGTTGa < 1:918743/69‑1 (MQ=255)
aCTCAACAGTGAACCATCTGGCGACCCGGTGCCATTGCCAGAACCCGCCTCAACGTTGCTAGATGTTGa < 1:1378990/69‑1 (MQ=255)
aCTCAACAGTGAACCATCTGGCGACCCGGTGCCATTGCCAGAACCCGCCTCAACGTTGCTAGATGTTGa < 1:2731508/69‑1 (MQ=255)
aCTCAACAGTGAACCATCTGGCGACCCGGTGCCATTGCCAGAACCCGCCTCAACGTTGCTAGATGTTGa < 1:3140664/69‑1 (MQ=255)
aCTCAACAGTGAACCATCTGGCGACCCGGTGCCATTGCCAGAACCCGCCTCAACGTTGCTAGATGTTGa < 1:2491926/69‑1 (MQ=255)
cTCAACAGTGAACCATCTGGCGACCCGGTGCCATTGCCAGAACCCGCCTCAACGTTGCTAGATGTTGa < 1:1872699/68‑1 (MQ=255)
cTCAACAGTGAACCATCTGGCGACCCGGTGCCATTGCCAGAACCCGCCTCAACGTTGCTAGATGTTGa < 1:2955465/68‑1 (MQ=255)
cTCAACAGTGAACCATCTGGCGACCCGGTGCCATTGCCAGAACCCGCCTCAACGTTGCTAGATGTTGa < 1:2800901/68‑1 (MQ=255)
cTCAACAGTGAACCATCTGGCGACCCGGTGCCATTGCCAGAACCCGCCTCAACGTTGCTAGATGTTGa < 1:494897/68‑1 (MQ=255)
cAGTGAACCATCTGGCGACCCGGTGCCATTGCCAGAACCCGCCTCAACGTTGCTAGATTTTGAACAGc > 1:2548203/1‑68 (MQ=255)
cAGTGAACCATCTGGCGACCCGGTGCCATTGCCAGAACCCGCCTCAACGTTGCTAGATGTTGAACAGc > 1:446865/1‑68 (MQ=255)
cAGTGAACCATCTGGCGACCCGGTGCCATTGCCAGAACCCGCCTCAACGTTGCTAGATGTTGAACAGc > 1:1088672/1‑68 (MQ=255)
cAGTGAACCATCTGGCGACCCGGTGCCATTGCCAGAACCCGCCTCAACGTTGCTAGATGTTGAACAGc > 1:833307/1‑68 (MQ=255)
cAGTGAACCATCTGGCGACCCGGTGCCATTGCCAGAACCCGCCTCAACGTTGCTAGATGTTGAACAGc > 1:2226866/1‑68 (MQ=255)
cAGTGAACCATCTGGCGACCCGGTGCCATTGCCAGAACCCGCCTCAACGTTGCTAGATGTTGAACAGc > 1:535454/1‑68 (MQ=255)
cAGTGAACCATCTGGCGACCCGGTGCCATTGCCAGAACCCGCCTCAACGTTGCTAGATGTTGAACAGc > 1:2305741/1‑68 (MQ=255)
cAGTGAACCATCTGGCGACCCGGTGCCATTGCCAGAACCCGCCTCAACGTTGCTAGATGTTGAACAGc > 1:2577156/1‑68 (MQ=255)
cAGTGAACCATCTGGCGACCCGGTGCCATTGCCAGAACCCGCCTCAACGTTGCTAGATGTTGAACAGc > 1:2759635/1‑68 (MQ=255)
cAGTGAACCATCTGGCGACCCGGTGCCATTGCCAGAACCCGCCTCAACGTTGCTAGATGTTGAACAGc > 1:2906270/1‑68 (MQ=255)
cAGTGAACCATCTGGCGACCCGGTGCCATTGCCAGAACCCGCCTCAACGTTGCTAGATGTTGAACAGc > 1:3034219/1‑68 (MQ=255)
cAGTGAACCATCTGGCGACCCGGTGCCATTGCCAGAACCCGCCTCAACGTTGCTAGATGTTGAACAGc > 1:3126153/1‑68 (MQ=255)
cAGTGAACCATCTGGCGACCCGGTGCCATTGCCAGAACCCGCCTCAACGTTGCTAGATGTTGAACAGc > 1:3148283/1‑68 (MQ=255)
cAGTGAACCATCTGGCGACCCGGTGCCATTGCCAGAACCCGCCTCAACGTTGCCAGATGTTGAACAGc > 1:1125806/1‑68 (MQ=255)
aGTGAACCGTCAGGCGATCCAGTGCCGTTGCCAGAACCTGCCTCAACGTTGCTGGATGTTGAACAGc > 1:1671635/1‑67 (MQ=255)
gAACCGTCAGGCGATCCAGTGCCGTTGCCAGAACCTGCCTCAACGTTGCTGGATGTTGAACAGCTTCAg > 1:3171000/1‑69 (MQ=255)
|
TACGCTATTTGCATCACCCACTCATCCTTACACACAAAAGCTACTCAACAGTGAACCGTCAGGCGATCCAGTGCCGTTGCCAGAACCTGCCTCAACGTTGCTGGATGTTGAACAGCTTCAG > minE/1408457‑1408577
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
