Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,419,735 |
T→C |
21.9% |
K76K (AAA→AAG) |
ccmH ← |
heme lyase, CcmH subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,419,735 | 0 | T | C | 21.9%
| 134.4
/ 29.7
| 100 | K76K (AAA→AAG) | ccmH | heme lyase, CcmH subunit |
| Reads supporting (aligned to +/- strand): ref base T (16/62); new base C (11/11); total (27/73) |
| Fisher's exact test for biased strand distribution p-value = 1.25e-02 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
Only 100 of 129 total aligned reads displayed.
AACGGCGGATCGTAAGTGACGAAGTTGCCGTAACGCGCCACCATATAATCGACAATCTCTTTTTTACTTTTACCTTCCTGCATCAGTTCATACACTTTCTGACGCAGGTCGGTGGCAATCATCG > minE/1419673‑1419796
|
aaCGGCGGATCGTAAGTGACGAAGTTGCCGTAACGCGCCACCATATAATCGACAATCTCTTttttact > 1:2627199/1‑68 (MQ=255)
aaCGGCGGATCGTAAGTGACGAAGTTGCCGTAACGCGCCACCATATAATCGACAATCTCTTttttact > 1:3296521/1‑68 (MQ=255)
aaCGGCGGATCGTAAGTGACGAAGTTGCCGTAACGCGCCACCATATAATCGACAATCTCTTttttact > 1:2163936/1‑68 (MQ=255)
aaCGGCGGATCGTAAGTGACGAAGTTGCCGTAACGCGCCACCATATAATCGACAATCTCTTttttact > 1:2964377/1‑68 (MQ=255)
aaCGGCGGATCGTAAGTGACGAAGTTGCCGTAACGCGCCACCATATAATCGACAATCTCTTttttact > 1:760950/1‑68 (MQ=255)
aaCGGCGGATCGTAAGTGACGAAGTTGCCGTAACGCGCCACCATATAATCGACAATCTCTTttttact > 1:1590455/1‑68 (MQ=255)
ggcggATCGTAAGTGACGAAGTTGCCGTAACGCGCCACCATATAATCGACAATCTCTTttttactttta < 1:402540/69‑1 (MQ=255)
ggcggATCGTAAGTGACGAAGTTGCCGTAACGCGCCACCATATAATCGACAATCTCTTttttactttta < 1:596101/69‑1 (MQ=255)
ggcggATCGTAAGTGACGAAGTTGCCGTAACGCGCCACCATATAATCGACAATCTCTTttttactttta < 1:110667/69‑1 (MQ=255)
ggcggATCGTAAGTGACGAAGTTGCCGTAACGCGCCACCATATAATCGACAATCTCTTttttactttta < 1:1249776/69‑1 (MQ=255)
ggcggATCGTAAGTGACGAAGTTGCCGTAACGCGCCACCATATAATCGACAATCTCTTttttactttta < 1:1513488/69‑1 (MQ=255)
ggcggATCGTAAGTGACGAAGTTGCCGTAACGCGCCACCATATAATCGACAATCTCTTttttactttt < 1:3284002/68‑1 (MQ=255)
ggcggATCGTAAGTGACGAAGTTGCCGTAACGCGCCACCATATAATCGACAATCTCTTttttactttt < 1:2375206/68‑1 (MQ=255)
ggcggATCGTAAGTGACGAAGTTGCCGTAACGCGCCACCATATAATCGACAATCTCTTttttactttt < 1:1824228/68‑1 (MQ=255)
ggcggATCGTAAGTGACGAAGTTGCCGTAACGCGCCACCATATAATCGACAATCTCTTttttactttt < 1:701521/68‑1 (MQ=255)
ggcggATCGTAAGTGACGAAGTTGCCGTAACGCGCCACCATATAATCGACAATCTCTTttttactttt < 1:1665122/68‑1 (MQ=255)
ggcggATCGTAAGTGACGAAGTTGCCGTAACGCGCCACCATATAATCGACAATCTCTTttttactttt < 1:3118359/68‑1 (MQ=255)
ggcggATCGTAAGTGACGAAGTTGCCGTAACGCGCCACCATATAATCGACAATCTCTTttttactttt < 1:3066105/68‑1 (MQ=255)
gATCGTAAGTGACGAAGTTGCCGTAACGCGCCACCATATAATCGACAATCTCTTTTTTACTTTTACCtt < 1:2039723/69‑1 (MQ=255)
gATCGTAAGTGACGAAGTTGCCGTAACGCGCCACCATATAATCGACAATCTCTTTTTTACTTTTACCtt < 1:2345075/69‑1 (MQ=255)
gATCGTAAGTGACGAAGTTGCCGTAACGCGCCACCATATAATCGACAATCTCTTTTTTACTTTTACCtt < 1:2025110/69‑1 (MQ=255)
gATCGTAAGTGACGAAGTTGCCGTAACGCGCCACCATATAATCGACAATCTCTTTTTTACTTTTACCtt < 1:3042074/69‑1 (MQ=255)
gATCGTAAGTGACGAAGTTGCCGTAACGCGCCACCATATAATCGACAATCTCTTTTTTACTTTTACCtt < 1:2942534/69‑1 (MQ=255)
gATCGTAAGTGACGAAGTTGCCGTAACGCGCCACCATATAATCGACAATCTCTTTTTTACTTTTACCtt < 1:1288622/69‑1 (MQ=255)
tCGTAAGTGACGAAGTTGCCGTAACGCGCCACCATATAATCGACAATCTCTTTTTTACTTTTACCTTcc < 1:885360/69‑1 (MQ=255)
tCGTAAGTGACGAAGTTGCCGTAACGCGCCACCATATAATCGACAATCTCTTTTTTACTTTTACCTTcc < 1:2069626/69‑1 (MQ=255)
tCGTAAGTGACGAAGTTGCCGTAACGCGCCACCATATAATCGACAATCTCTTTTTTACTTTTACCTTcc < 1:295077/69‑1 (MQ=255)
tCGTAAGTGACGAAGTTGCCGTAACGCGCCACCATATAATCGACAATCTCTTTTTTACTTTTACCTTcc < 1:1916014/69‑1 (MQ=255)
tCGTAAGTGACGAAGTTGCCGTAACGCGCCACCATATAATCGACAATCTCTTTTTTACTTTTACCTTcc < 1:1184271/69‑1 (MQ=255)
tCGTAAGTGACGAAGTTGCCGTAACGCGCCACCATATAATCGACAATCTCTTTTTTACTTTTACCTTcc < 1:1590404/69‑1 (MQ=255)
gAAGTTGCCGTAACGCGCCACCATATAATCGACAATCTCTTTTTTACTTTTACCTTCCTGCATCAGtt > 1:177699/1‑68 (MQ=255)
gAAGTTGCCGTAACGCGCCACCATATAATCGACAATCTCTTTTTTACTTTTACCTTCCTGCATCAGtt > 1:1962300/1‑68 (MQ=255)
gAAGTTGCCGTAACGCGCCACCATATAATCGACAATCTCTTTCTTACTTTTGCCTTCCTGCATCAGtt > 1:2570464/1‑68 (MQ=255)
gAAGTTGCCGTAACGCGCCACCATATAATCGACAATCTCTTTCTTACTTTTGCCTTCCTGCATCAGtt > 1:603443/1‑68 (MQ=255)
gAAGTTGCCGTAACGCGCCACCATATAATCGACAATCTCTTTCTTACTTTTGCCTTCCTGCATCAGtt > 1:2335753/1‑68 (MQ=255)
gAAGTTGCCGTAACGCGCCACCATATAATCGACAATCTCTTTCTTACTTTTGCCTTCCTGCATCAGtt > 1:1092605/1‑68 (MQ=255)
gAAGTTGCCGTAACGCGCCACCATATAATCGACAATCTCTTTCTTACTTTTGCCTTCCTGCATCAGtt > 1:31468/1‑68 (MQ=255)
gAAGTTGCCGTAACGCGCCACCATATAATCGACAATCTCTTTCTTACTTTTGCCTTCCTGCATCAGtt > 1:1547760/1‑68 (MQ=255)
gAAGTTGCCGTAACGCGCCACCATATAATCGACAATCTCTTTCTTACTTTTGCCTTCCTGCATCAGtt > 1:718581/1‑68 (MQ=255)
gAAGTTGCCGTAACGCGCCACCATATAATCGACAATCTCTTTCTTACTTTTGCCTTCCTGCATCAGtt > 1:744771/1‑68 (MQ=255)
gAAGTTGCCGTAACGCGCCACCATATAATCGACAATCTCTTTCTTACTTTTGCCTTCCTGCATCAGtt > 1:159982/1‑68 (MQ=255)
aaGTTGCCGTAACGCGCCACCATATAATCGACAATCTCTTTTTTACTTTTACCTTCCTGCATCAGTTCa < 1:3035406/69‑1 (MQ=255)
aaGTTGCCGTAACGCGCCACCATATAATCGACAATCTCTTTTTTACTTTTACCTTCCTGCATCAGTTCa < 1:1670858/69‑1 (MQ=255)
aaGTTGCCGTAACGCGCCACCATATAATCGACAATCTCTTTTTTACTTTTACCTTCCTGCATCAGTTCa < 1:33829/69‑1 (MQ=255)
aaGTTGCCGTAACGCGCCACCATATAATCGACAATCTCTTTTTTACTTTTACCTTCCTGCATCAGTTCa < 1:2303139/69‑1 (MQ=255)
aaGTTGCCGTAACGCGCCACCATATAATCGACAATCTCTTTTTTACTTTTACCTTCCTGCATCAGTTCa < 1:543335/69‑1 (MQ=255)
aaGTTGCCGTAACGCGCCACCATATAATCGACAATCTCTTTTTTACTTTTACCTTCCTGCATCAGTTCa < 1:2383421/69‑1 (MQ=255)
aaGTTGCCGTAACGCGCCACCATATAATCGACAATCTCTTTTTTACTTTTACCTTCCTGCATCAGTTCa < 1:2059535/69‑1 (MQ=255)
aGTTGCCGTAACGCGCCACCATATAATCGACAATCTCTTTTTTACTTTTACCTTCCTGCATCAGTTCa < 1:1514580/68‑1 (MQ=255)
gTTGCCGTAACGCGCCACCATATAATCGACAATCTCTTTTTTACTTTTACCTTCCTGCATCAGTTCAt > 1:1045828/1‑68 (MQ=255)
gTTGCCGTAACGCGCCACCATATAATCGACAATCTCTTTTTTACTTTTACCTTCCTGCATCAGTTCATa < 1:1001195/69‑1 (MQ=255)
gTTGCCGTAACGCGCCACCATATAATCGACAATCTCTTTTTTACTTTTACCTTCCTGCATCAGTTCATa < 1:2888361/69‑1 (MQ=255)
gTTGCCGTAACGCGCCACCATATAATCGACAATCTCTTTTTTACTTTTACCTTCCTGCATCAGTTCATa < 1:612217/69‑1 (MQ=255)
ttGCCGTAACGCGCCACCATATAATCGACAATCTCTTTTTTACTTTTACCTTCCTGCATCAGTTCATac > 1:2757449/1‑69 (MQ=255)
ttGCCGTAACGCGCCACCATATAATCGACAATCTCTTTTTTACTTTTACCTTCCTGCATCAGTTCATac > 1:2326432/1‑69 (MQ=255)
tGCCGTAACGCGCCACCATATAATCGACAATCTCTTTTTTACTTTTACCTTcc < 1:2417185/53‑1 (MQ=255)
tGCCGTAACGCGCCACCATATAATCGACAATCTCTTTTTTACTTTTACCTTCCTGCATCAGTTCATaca < 1:3027505/69‑1 (MQ=255)
tGCCGTAACGCGCCACCATATAATCGACAATCTCTTTTTTACTTTTACCTTCCTGCATCAGTTCATaca < 1:2953822/69‑1 (MQ=255)
tGCCGTAACGCGCCACCATATAATCGACAATCTCTTTTTTACTTTTACCTTCCTGCATCAGTTCATaca < 1:93517/69‑1 (MQ=255)
tGCCGTAACGCGCCACCATATAATCGACAATCTCTTTTTTACTTTTACCTTCCTGCATCAGTTCATaca < 1:2550407/69‑1 (MQ=255)
tGCCGTAACGCGCCACCATATAATCGACAATCTCTTTTTTACTTTTACCTTCCTGCATCAGTTCATaca < 1:592354/69‑1 (MQ=255)
tGCCGTAACGCGCCACCATATAATCGACAATCTCTTTTTTACTTTTACCTTCCTGCATCAGTTCATaca < 1:2364670/69‑1 (MQ=255)
tGCCGTAACGCGCCACCATATAATCGACAATCTCTTTTTTACTTTTACCTTCCTGCATCAGTTCATaca < 1:2291835/69‑1 (MQ=255)
tGCCGTAACGCGCCACCATATAATCGACAATCTCTTTTTTACTTTTACCTTCCTGCATCAGTTCATaca < 1:2187697/69‑1 (MQ=255)
tGCCGTAACGCGCCACCATATAATCGACAATCTCTTTTTTACTTTTACCTTCCTGCATCAGTTCATaca < 1:635797/69‑1 (MQ=255)
tGCCGTAACGCGCCACCATATAATCGACAATCTCTTTTTTACTTTTACCTTCCTGCATCAGTTCATaca < 1:2017462/69‑1 (MQ=255)
tGCCGTAACGCGCCACCATATAATCGACAATCTCTTTTTTACTTTTACCTTCCTGCATCAGTTCATaca < 1:714494/69‑1 (MQ=255)
tGCCGTAACGCGCCACCATATAATCGACAATCTCTTTTTTACTTTTACCTTCCTGCATCAGTTCATaca < 1:1625201/69‑1 (MQ=255)
tGCCGTAACGCGCCACCATATAATCGACAATCTCTTTTTTACTTTTACCTTCCTGCATCAGTTCATaca < 1:1010229/69‑1 (MQ=255)
tGCCGTAACGCGCCACCATATAATCGACAATCTCTTTTTTACTTTTACCTTCCTGCATCAGTTCATaca < 1:109562/69‑1 (MQ=255)
gCCGTAACGCGCCACCATATAATCGACAATCTCTTTTTTACTTTTACCt < 1:328509/49‑1 (MQ=255)
gCCGTAACGCGCCACCATATAATCGACAATCTCTTTTTTACTTTTACCt < 1:2617668/49‑1 (MQ=255)
gCCGTAACGCGCCACCATATAATCGACAATCTCTTTTTTACTTTTACCt < 1:3153804/49‑1 (MQ=255)
gCCGTAACGCGCCACCATATAATCGACAATCTCTTTTTTACTTTTACCt < 1:890625/49‑1 (MQ=255)
gCCGTAACGCGCCACCATATAATCGACAATCTCTTTTTTACTTTTACCt < 1:1410134/49‑1 (MQ=255)
gCCGTAACGCGCCACCATATAATCGACAATCTCTTTTTTACTTTTACCt < 1:1516007/49‑1 (MQ=255)
gCCGTAACGCGCCACCATATAATCGACAATCTCTTTTTTACTTTTACCt < 1:352796/49‑1 (MQ=255)
cGTAACGCGCCACCATATAATCGACAATCTCTTttttactttta < 1:2376443/44‑1 (MQ=255)
aaCGCGCCACCATATAATCGACAATCTCTTTTTTACTTTTACCTTCCTGCATCAGTTCATACACTTTCt < 1:1633622/69‑1 (MQ=255)
aaCGCGCCACCATATAATCGACAATCTCTTTTTTACTTTTACCTTCCTGCATCAGTTCATACACTTTCt < 1:248604/69‑1 (MQ=255)
caTATAATCGACAATCTCTTTTTTACTTTTACCTTCCTGCATCAGTTCATACACTTTCTGACGCAGGTc < 1:1289300/69‑1 (MQ=255)
taATCGACAATCTCTTTTTTACTTTTACCTTCCTGCATCAGTTCATACACTTTCTGACGCa > 1:2284661/1‑61 (MQ=255)
taATCGACAATCTCTTTTTTACTTTTACCTTCCTGCATCAGTTCATACACTTTCTGACGCAGGTCGGt > 1:2052239/1‑68 (MQ=255)
taATCGACAATCTCTTTTTTACTTTTACCTTCCTGCATCAGTTCATACACTTTCTGACGCAGGTCGGt > 1:1009535/1‑68 (MQ=255)
taATCGACAATCTCTTTTTTACTTTTACCTTCCTGCATCAGTTCATACACTTTCTGACGCAGGTCGGt > 1:88230/1‑68 (MQ=255)
taATCGACAATCTCTTTTTTACTTTTACCTTCCTGCATCAGTTCATACACTTTCTGACGCAGGTCGGt > 1:797635/1‑68 (MQ=255)
taATCGACAATCTCTTTTTTACTTTTACCTTCCTGCATCAGTTCATACACTTTCTGACGCAGGTCGGt > 1:3287238/1‑68 (MQ=255)
taATCGACAATCTCTTTTTTACTTTTACCTTCCTGCATCAGTTCATACACTTTCTGACGCAGGTCGGt > 1:615546/1‑68 (MQ=255)
taATCGACAATCTCTTTTTTACTTTTACCTTCCTGCATCAGTTCATACACTTTCTGACGCAGGTCGGt > 1:2154848/1‑68 (MQ=255)
tctcTTTCTTACTTTTGCCTTCCTGCATCAGTTCATACACTTTCTGGCGCAGGTCGGTGGCAATCATCg < 1:1798670/69‑1 (MQ=255)
tctcTTTCTTACTTTTGCCTTCCTGCATCAGTTCATACACTTTCTGGCGCAGGTCGGTGGCAATCATCg < 1:171895/69‑1 (MQ=255)
tctcTTTCTTACTTTTGCCTTCCTGCATCAGTTCATACACTTTCTGGCGCAGGTCGGTGGCAATCATCg < 1:1702642/69‑1 (MQ=255)
tctcTTTCTTACTTTTGCCTTCCTGCATCAGTTCATACACTTTCTGGCGCAGGTCGGTGGCAATCATCg < 1:1475009/69‑1 (MQ=255)
tctcTTTCTTACTTTTGCCTTCCTGCATCAGTTCATACACTTTCTGGCGCAGGTCGGTGGCAATCATCg < 1:2556423/69‑1 (MQ=255)
tctcTTTCTTACTTTTGCCTTCCTGCATCAGTTCATACACTTTCTGGCGCAGGTCGGTGGCAATCATCg < 1:2566374/69‑1 (MQ=255)
tctcTTTCTTACTTTTGCCTTCCTGCATCAGTTCATACACTTTCTGGCGCAGGTCGGTGGCAATCATCg < 1:1331726/69‑1 (MQ=255)
tctcTTTCTTACTTTTGCCTTCCTGCATCAGTTCATACACTTTCTGGCGCAGGTCGGTGGCAATCATCg < 1:2875215/69‑1 (MQ=255)
ctctTTCTTACTTTTGCCTTCCTGCATCAGTTCATACACTTTCTGGCGCAGGTCGGTGGCAATCATCg < 1:2092013/68‑1 (MQ=255)
ctctTTCTTACTTTTGCCTTCCTGCATCAGTTCATACACTTTCTGGCGCAGGTCGGTGGCAATCATCg < 1:2550091/68‑1 (MQ=255)
ctctTTCTTACTTTTGCCTTCCTGCATCAGTTCATACACTTTCTGGCGCAGGTCGGTGGCAATCATCg < 1:2716300/68‑1 (MQ=255)
|
AACGGCGGATCGTAAGTGACGAAGTTGCCGTAACGCGCCACCATATAATCGACAATCTCTTTTTTACTTTTACCTTCCTGCATCAGTTCATACACTTTCTGACGCAGGTCGGTGGCAATCATCG > minE/1419673‑1419796
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
