Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,474,270 |
T→C |
28.2% |
A618A (GCT→GCC) |
nrdA → |
ribonucleoside diphosphate reductase 1, alpha subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,474,270 | 0 | T | C | 28.2%
| 91.6
/ 40.0
| 92 | A618A (GCT→GCC) | nrdA | ribonucleoside diphosphate reductase 1, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base T (14/52); new base C (19/7); total (33/59) |
| Fisher's exact test for biased strand distribution p-value = 6.12e-06 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.72e-01 |
ACGACTGGGAAGCTCTGCGTGAGTCAATCAAAACGCACGGTCTGCGTAACTCCACGCTTTCTGCTCTGATGCCGTCCGAGACTTCTTCGCAGATCTCTAACGCCACTAACGGTATTGAACCGCC > minE/1474206‑1474329
|
aCGACTGGGAAGCTCTGCGTGAGTCAATCAAAACGCACGGTCTGCGTAACTCCACGCTTTCTGCTCTGa > 1:2088796/1‑69 (MQ=255)
tGGGAAGCTCTGCGTGAGTCAATCAAAACGCACGGTCTGCGTAACTCCACGCTTTCTGctct > 1:2716776/1‑62 (MQ=255)
tGGGAAGCTCTGCGTGAGTCAATCAAAACGCACGGTCTGCGTAACTCCACGCTTTCTGctct > 1:1491506/1‑62 (MQ=255)
aGCTCTGCGTGAGTCAATCAAAACGCACGGTCTGCGTAACTCCACGCTTTCTGCTCTGATGCCGTCCga < 1:890270/69‑1 (MQ=255)
aGCTCTGCGTGAGTCAATCAAAACGCACGGTCTGCGTAACTCCACGCTTTCTGCTCTGATGCCGTCCga < 1:1834360/69‑1 (MQ=255)
aGCTCTGCGTGAGTCAATCAAAACGCACGGTCTGCGTAACTCCACGCTTTCTGCTCTGATGCCGTCCga < 1:2735451/69‑1 (MQ=255)
aGCTCTGCGTGAGTCAATCAAAACGCACGGTCTGCGTAACTCCACGCTTTCTGCTCTGATGCCGTCCga < 1:1760106/69‑1 (MQ=255)
aGCTCTGCGTGAGTCAATCAAAACGCACGGTCTGCGTAACTCCACGCTTTCTGCTCTGATGCCGTCCga < 1:1957585/69‑1 (MQ=255)
aGCTCTGCGTGAGTCAATCAAAACGCACGGTCTGCGTAACTCCACGCTTTCTGCTCTGATGCCGTCCga < 1:3077543/69‑1 (MQ=255)
aGCTCTGCGTGAGTCAATCAAAACGCACGGTCTGCGTAACTCCACGCTTTCTGCTCTGATGCCGTCCga < 1:1740838/69‑1 (MQ=255)
aGCTCTGCGTGAGTCAATCAAAACGCACGGTCTGCGTAACTCCACGCTTTCTGCTCTGATGCCGTCCga < 1:335529/69‑1 (MQ=255)
aGCTCTGCGTGAGTCAATCAAAACGCACGGTCTGCGTAACTCCACGCTTTCTGCTCTGATGCCGTCCga < 1:1611049/69‑1 (MQ=255)
aGCTCTGCGTGAGTCAATCAAAACGCACGGTCTGCGTAACTCCACGCTTTCTGCTCTGATGCCGTCCga < 1:1582970/69‑1 (MQ=255)
aGCTCTGCGTGAGTCAATCAAAACGCACGGTCTGCGTAACTCCACGCTTTCTGCTCTGATGCCGTCCga < 1:636405/69‑1 (MQ=255)
aGCTCTGCGTGAGTCAATCAAAACGCACGGTCTGCGTAACTCCACGCTTTCTGCTCTGATGCCGTCCga < 1:1060106/69‑1 (MQ=255)
aGCTCTGCGTGAGTCAATCAAAACGCACGGTCTGCGTAACTCCACGCTTTCTGCTCTGATGCCGTCCga < 1:1141173/69‑1 (MQ=255)
aGCTCTGCGTGAGTCAATCAAAACGCACGGTCTGCGTAACTCCACGCTTTCTGCTCTGATGCCGTCCga < 1:1366440/69‑1 (MQ=255)
aGCTCTGCGTGAGTCAATCAAAACGCACGGTCTGCGTAACTCCACGCTTTCTGCTCTGATGCCGTCCga < 1:1394872/69‑1 (MQ=255)
aGCTCTGCGTGAGTCAATCAAAACGCACGGTCTGCGTAACTCCACGCTTTCTGCTCTGATGCCGTCCga < 1:79213/69‑1 (MQ=255)
tctGCGTGAGTCAATCAAAACGCACGGTCTGCGTAACTCCACGCTTTCTGCTCTGATGCCGTCCGAGAc < 1:2122557/69‑1 (MQ=255)
tctGCGTGAGTCAATCAAAACGCACGGTCTGCGTAACTCCACGCTTTCTGCTCTGATGCCGTCCGAGAc < 1:2812995/69‑1 (MQ=255)
tctGCGTGAGTCAATCAAAACGCACGGTCTGCGTAACTCCACGCTTTCTGCTCTGATGCCGTCCGAGAc < 1:1879203/69‑1 (MQ=255)
tctGCGTGAGTCAATCAAAACGCACGGTCTGCGTAACTCCACGCTTTCTGCTCTGATGCCGTCCGAGAc < 1:1126778/69‑1 (MQ=255)
tctGCGTGAGTCAATCAAAACGCACGGTCAGCGTAACTCCACGCTTTCTGCTCTGATGCCGTCCGAGAc < 1:1373330/69‑1 (MQ=255)
gTCAATCAAAACGCACGGCCTGCGTAACTCCACGCTTTCTGCCCTGa > 1:2491554/1‑47 (MQ=255)
gTCAATCAAAACGCACGGCCTGCGTAACTCCACGCTTTCTGCCCTGa > 1:251219/1‑47 (MQ=255)
gTCAATCAAAACGCACGGCCTGCGTAACTCCACGCTTTCTGCCCTGa > 1:2434097/1‑47 (MQ=255)
gTCAATCAAAACGCACGGCCTGCGTAACTCCACGCTTTCTGCCCTGa > 1:1963191/1‑47 (MQ=255)
gTCAATCAAAACGCACGGCCTGCGTAACTCCACGCTTTCTGCCCTGa > 1:2330984/1‑47 (MQ=255)
gTCAATCAAAACGCACGGCCTGCGTAACTCCACGCTTTCTGCCCTGa > 1:2640794/1‑47 (MQ=255)
gTCAATCAAAACGCACGGCCTGCGTAACTCCACGCTTTCTGCCCTGa > 1:2431377/1‑47 (MQ=255)
gTCAATCAAAACGCACGGCCTGCGTAACTCCACGCTTTCTGCCCTGa > 1:2116876/1‑47 (MQ=255)
gTCAATCAAAACGCACGGCCTGCGTAACTCCACGCTTTCTGCCCTGa > 1:2386763/1‑47 (MQ=255)
gTCAATCAAAACGCACGGCCTGCGTAACTCCACGCTTTCTGCCCTGa > 1:2839530/1‑47 (MQ=255)
gTCAATCAAAACGCACGGCCTGCGTAACTCCACGCTTTCTGCCCTGa > 1:1753649/1‑47 (MQ=255)
gTCAATCAAAACGCACGGCCTGCGTAACTCCACGCTTTCTGCCCTGa > 1:2854378/1‑47 (MQ=255)
gTCAATCAAAACGCACGGCCTGCGTAACTCCACGCTTTCTGCCCTGa > 1:1669939/1‑47 (MQ=255)
gTCAATCAAAACGCACGGCCTGCGTAACTCCACGCTTTCTGCCCTGa > 1:1628732/1‑47 (MQ=255)
gTCAATCAAAACGCACGGCCTGCGTAACTCCACGCTTTCTGCCCTGa > 1:404165/1‑47 (MQ=255)
gTCAATCAAAACGCACGGCCTGCGTAACTCCACGCTTTCTGCCCTGa > 1:462645/1‑47 (MQ=255)
gTCAATCAAAACGCACGGCCTGCGTAACTCCACGCTTTCTGCCCTGa > 1:491430/1‑47 (MQ=255)
gTCAATCAAAACGCACGGCCTGCGTAACTCCACGCTTTCTGCCCTGa > 1:758667/1‑47 (MQ=255)
gTCAATCAAAACGCACGGCCTGCGTAACTCCACGCTTTCTGCCCTGa > 1:1304599/1‑47 (MQ=255)
tcaatcaaAACGCACGGTCTGCGTATCTCCACGCTTTCTGCTCTGATGCCGTCCGAGACTTCTTCGCAg < 1:2741199/69‑1 (MQ=255)
tcaatcaaAACGCACGGTCTGCGTAACTCCACGCTTTCTGCTCTGATGCCGTCCGAGActtctt > 1:3298467/1‑64 (MQ=255)
tcaatcaaAACGCACGGTCTGCGTAACTCCACGCTTTCTGCTCTGATGCCGTCCGAGActtctt > 1:2413198/1‑64 (MQ=255)
tcaatcaaAACGCACGGTCTGCGTAACTCCACGCTTTCTGCTCTGATGCCGTCCGAGACTTCTTCGCAg < 1:2625544/69‑1 (MQ=255)
tcaatcaaAACGCACGGTCTGCGTAACTCCACGCTTTCTGCTCTGATGCCGTCCGAGACTTCTTCGCAg < 1:1037210/69‑1 (MQ=255)
tcaatcaaAACGCACGGTCTGCGTAACTCCACGCTTTCTGCTCTGATGCCGTCCGAGACTTCTTCGCAg < 1:1981933/69‑1 (MQ=255)
tcaatcaaAACGCACGGTCTGCGTAACTCCACGCTTTCTGCTCTGATGCCGTCCGAGACTTCTTCGCAg < 1:1586136/69‑1 (MQ=255)
tcaatcaaAACGCACGGTCTGCGTAACTCCACGCTTTCTGCTCTGATGCCGTCCGAGACTTCTTCGCAg < 1:1444281/69‑1 (MQ=255)
caaAACGCACGGTCTGCGTAACTCCACGCTTTCTGCTCTGATGCCGTCCGAGAct > 1:2113669/1‑55 (MQ=255)
caaAACGCACGGTCTGCGTAACTCCACGCTTTCTGCTCTGATGCCGTCCGAGACTTCTTCGCAGAtct > 1:2558557/1‑68 (MQ=255)
caaAACGCACGGTCTGCGTAACTCCACGCTTTCTGCTCTGATGCCGTCCGAGACTTCTTCGCAGAtct > 1:78160/1‑68 (MQ=255)
caaAACGCACGGTCTGCGTAACTCCACGCTTTCTGCTCTGATGCCGTCCGAGACTTCTTCGCAGAtct > 1:1850090/1‑68 (MQ=255)
caaAACGCACGGTCTGCGTAACTCCACGCTTTCTGCTCTGATGCCGTCCGAGACTTCTTCGCAGAtct > 1:2934951/1‑68 (MQ=255)
caaAAAGCACGGTCTGCGTAACTCCACGCTTTCTGCTCTGATGCCGTCCGAGACTTCTTCGCAGAtct > 1:1010217/1‑68 (MQ=255)
aaaaCGCACGGTCTGCGTAACTCCACGCTTTCTGCTCTGATGCCGTCCGAGACTTCTTCGCAGATctct < 1:2389219/69‑1 (MQ=255)
aaaaCGCACGGTCTGCGTAACTCCACGCTTTCTGCTCTGATGCCGTCCGAGACTTCTTCGCAGATctct < 1:410571/69‑1 (MQ=255)
aaaaCGCACGGTCTGCGTAACTCCACGCTTTCTGCTCTGATGCCGTCCGAGACTTCTTCGCAGATctct < 1:663368/69‑1 (MQ=255)
aaCGCACGGTCTGCGTAACTCCACGCTTTCTGCTCTGATGCCGTCCGAGACTTCTTCGCAg < 1:3067344/61‑1 (MQ=255)
cGGTCTGCGTAACTCCACGCTTTCTGCTCTGATGCCGTCCGAGACTTCTTCGCAGATCTCTAACGCCa > 1:3154077/1‑68 (MQ=255)
ggTCTGCGTAACTCCACGCTTTCTGCTCTGATGCCGTCCGAGACTTCTTCGCAGATCTCTAACGCCACt < 1:629922/69‑1 (MQ=255)
ggTCTGCGTAACTCCACGCTTTCTGCTCTGATGCCGTCCGAGACTTCTTCGCAGATCTCTAACGCCACt < 1:545371/69‑1 (MQ=255)
ggTCTGCGTAACTCCACGCTTTCTGCTCTGATGCCGTCCGAGACTTCTTCGCAGATCTCTAACGCCACt < 1:521443/69‑1 (MQ=255)
ggTCTGCGTAACTCCACGCTTTCTGCTCTGATGCCGTCCGAGACTTCTTCGCAGATCTCTAACGCCACt < 1:711531/69‑1 (MQ=255)
ggTCTGCGTAACTCCACGCTTTCTGCTCTGATGCCGTCCGAGACTTCTTCGCAGATCTCTAACGCCACt < 1:1431867/69‑1 (MQ=255)
ggTCTGCGTAACTCCACGCTTTCTGCTCTGATGCCGTCCGAGACTTCTTCGCAGATCTCTAACGCCACt < 1:777958/69‑1 (MQ=255)
ggTCTGCGTAACTCCACGCTTTCTGCTCTGATGCCGTCCGAGACTTCTTCGCAGATCTCTAACGCCACt < 1:787585/69‑1 (MQ=255)
ggTCTGCGTAACTCCACGCTTTCTGCTCTGATGCCGTCCGAGACTTCTTCGCAGATCTCTAACGCCACt < 1:81101/69‑1 (MQ=255)
ggTCTGCGTAACTCCACGCTTTCTGCTCTGATGCCGTCCGAGACTTCTTCGCAGATCTCTAACGCCACt < 1:960723/69‑1 (MQ=255)
ggTCTGCGTAACTCCACGCTTTCTGCTCTGATGCCGTCCGAGACTTCTTCGCAGATCTCTAACGCCACt < 1:3133039/69‑1 (MQ=255)
ggTCTGCGTAACTCCACGCTTTCTGCTCTGATGCCGTCCGAGACTTCTTCGCAGATCTCTAACGCCACt < 1:1978789/69‑1 (MQ=255)
ggTCTGCGTAACTCCACGCTTTCTGCTCTGATGCCGTCCGAGACTTCTTCGCAGATCTCTAACGCCACt < 1:1934157/69‑1 (MQ=255)
ggTCTGCGTAACTCCACGCTTTCTGCTCTGATGCCGTCCGAGACTTCTTCGCAGATCTCTAACGCCACt < 1:1757648/69‑1 (MQ=255)
gTCTGCGTAACTCCACGCTTTCTGCTCTGATGCCGTCCGAGACTTCTTCGCAGATCTCTAACGCCACt < 1:2882806/68‑1 (MQ=255)
tCTGCGTAACTCCACGCTTTCTGCTCTGATGCCGTCCGAGACTTCTTCGCAGATCTCTAACGCCACt < 1:2960102/67‑1 (MQ=255)
tCTGCGTAACTCCACGCTTTCTGCTCTGATGCCGTCCGAGACTTCTTCGCAGATCTCTAACGCCACt < 1:1773463/67‑1 (MQ=255)
cTGCGTAACTCCACGCTTTCTGCTCTGATGCCGTCCga < 1:1752936/38‑1 (MQ=255)
aCTCCACGCTTTCTGCTCTGATGCCGTCCGAGACTTCTTCGCAGATCTCTAACGCCACTAACGGTATTg > 1:2250796/1‑69 (MQ=255)
aCTCCACGCTTTCTGCTCTGATGCCGTCCGAGACTTCTTCGCAGATCTCTAACGCCACTAACGGTATTg > 1:1412057/1‑69 (MQ=255)
aCTCCACGCTTTCTGCTCTGATGCCGTCCGAGACTTCTTCGCAGATCTCTAACGCCACTAACGGTATTg < 1:2041705/69‑1 (MQ=255)
aCTCCACGCTTTCTGCTCTGATGCCGTCCGAGACTTCTTCGCAGATCTCTAACGCCACTAACGGTATTg > 1:1756553/1‑69 (MQ=255)
aCTCCACGCTTTCTGCTCTGATGCCGTCCGAGACTTCTTCGCAGATCTCTAACGCCACTAACGGTATTg > 1:2933416/1‑69 (MQ=255)
aCTCCACGCTTTCTGCTCTGATGCCGTCCGAGACTTCTTCGCAGATCTCTAACGCCACTAACGGTATTg < 1:1608228/69‑1 (MQ=255)
gCTTTCTGCCCTGATGCCGTCCGAGACTTCTTCGCAGATCTCTAACGCCACTAACGGTATTGAAccgcc < 1:1443568/69‑1 (MQ=255)
gCTTTCTGCCCTGATGCCGTCCGAGACTTCTTCGCAGATCTCTAACGCCACTAACGGTATTGAAccgcc < 1:1831953/69‑1 (MQ=255)
gCTTTCTGCCCTGATGCCGTCCGAGACTTCTTCGCAGATCTCTAACGCCACTAACGGTATTGAAccgcc < 1:1752953/69‑1 (MQ=255)
gCTTTCTGCCCTGATGCCGTCCGAGACTTCTTCGCAGATCTCTAACGCCACTAACGGTATTGAAccgcc < 1:1670267/69‑1 (MQ=255)
gCTTTCTGCCCTGATGCCGTCCGAGACTTCTTCGCAGATCTCTAACGCCACTAACGGTATTGAAccgcc < 1:833180/69‑1 (MQ=255)
gCTTTCTGCCCTGATGCCGTCCGAGACTTCTTCGCAGATCTCTAACGCCACTAACGGTATTGAAccgcc < 1:2107158/69‑1 (MQ=255)
gCTTTCTGCCCTGATGCCGTCCGAGACTTCTTCGCAGATCTCTAACGCCACTAACGGTATTGAAccgcc < 1:1681329/69‑1 (MQ=255)
tttCTGCTCTGAAGCCGTCCGAGACTTCTTCGCAg < 1:2460176/35‑1 (MQ=255)
ttCTGCTCTGATGCCGTCCGAGACTTCTTCGCAGATctct < 1:78474/40‑1 (MQ=255)
|
ACGACTGGGAAGCTCTGCGTGAGTCAATCAAAACGCACGGTCTGCGTAACTCCACGCTTTCTGCTCTGATGCCGTCCGAGACTTCTTCGCAGATCTCTAACGCCACTAACGGTATTGAACCGCC > minE/1474206‑1474329
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
