Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,493,288 |
G→A |
63.1% |
Y47Y (TAC→TAT) |
nuoH ← |
NADH:ubiquinone oxidoreductase, membrane subunit H |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,493,288 | 0 | G | A | 63.1%
| 22.9
/ 27.1
| 46 | Y47Y (TAC→TAT) | nuoH | NADH:ubiquinone oxidoreductase, membrane subunit H |
| Reads supporting (aligned to +/- strand): ref base G (6/11); new base A (9/20); total (15/31) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01 |
AACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACA > minE/1493226‑1493354
|
aaCATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACgg < 1:1664660/69‑1 (MQ=255)
aCATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACgg < 1:3341502/68‑1 (MQ=255)
aCATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACgg < 1:2734104/68‑1 (MQ=255)
cATTTTGATCATGTCCTCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCATAACGGtt > 1:2754968/1‑69 (MQ=255)
cATTTTGATCATGTCCTCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCATAACGGtt > 1:962431/1‑69 (MQ=255)
cATTTTGATCATGTCCTCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCATAACGGtt > 1:954771/1‑69 (MQ=255)
cATTTTGATCATGTCCTCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCATAACGGtt > 1:2767180/1‑69 (MQ=255)
cATTTTGATCATGTCCTCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCATAACGGtt > 1:2716391/1‑69 (MQ=255)
cATTTTGATCATGTCCTCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCATAACGGtt > 1:2266703/1‑69 (MQ=255)
cATTTTGATCATGTCCTCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCATAACGGtt > 1:1917553/1‑69 (MQ=255)
cATTTTGATCATGTCCTCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCATAACGGtt > 1:191301/1‑69 (MQ=255)
cATTTTGATCATGTCCTCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCATAACGGtt > 1:155987/1‑69 (MQ=255)
tCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGAccc < 1:825354/69‑1 (MQ=255)
tCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGAccc > 1:2273047/1‑69 (MQ=255)
tCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGAccc < 1:366604/69‑1 (MQ=255)
tCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGAccc < 1:127405/69‑1 (MQ=255)
tCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGAccc < 1:2827237/69‑1 (MQ=255)
tCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGAccc > 1:3073139/1‑69 (MQ=255)
tCCGCAACCAGCTGGAGAGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCt > 1:2122215/1‑58 (MQ=255)
ccGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGAccc < 1:1523239/68‑1 (MQ=255)
cAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATg < 1:1184871/69‑1 (MQ=255)
cAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATg < 1:1512946/69‑1 (MQ=255)
cAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATg < 1:593048/69‑1 (MQ=255)
tAGGTCCATAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCACAgg < 1:1260344/69‑1 (MQ=255)
tAGGTCCATAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCACAgg < 1:1850053/69‑1 (MQ=255)
tAGGTCCATAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCACAgg < 1:3404406/69‑1 (MQ=255)
tAGGTCCATAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCACAgg < 1:371625/69‑1 (MQ=255)
tAGGTCCATAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCACAgg < 1:405278/69‑1 (MQ=255)
tAGGTCCATAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCACAgg < 1:439410/69‑1 (MQ=255)
tAGGTCCATAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCACAgg < 1:622235/69‑1 (MQ=255)
tAGGTCCATAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCACAgg < 1:623959/69‑1 (MQ=255)
tAGGTCCATAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCACAgg < 1:1188458/69‑1 (MQ=255)
tAGGTCCATAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCACAgg < 1:3332184/69‑1 (MQ=255)
tAGGTCCATAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCACAgg < 1:3250976/69‑1 (MQ=255)
tAGGTCCATAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCACAgg < 1:3209761/69‑1 (MQ=255)
tAGGTCCATAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCACAgg < 1:3207521/69‑1 (MQ=255)
tAGGTCCATAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCACAgg < 1:1345456/69‑1 (MQ=255)
tAGGTCCATAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCACAgg < 1:1356532/69‑1 (MQ=255)
tAGGTCCATAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCACAgg < 1:2415172/69‑1 (MQ=255)
tAGGTCCATAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCACAgg < 1:2180312/69‑1 (MQ=255)
tAGGTCCATAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCACAgg < 1:2159548/69‑1 (MQ=255)
tAGGTCCATAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCACAgg < 1:1557010/69‑1 (MQ=255)
ggTCCATAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCACAgg < 1:1034254/67‑1 (MQ=255)
cGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACa > 1:3088021/1‑68 (MQ=255)
cGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACa > 1:3021693/1‑68 (MQ=255)
cGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACa > 1:585965/1‑68 (MQ=255)
|
AACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACA > minE/1493226‑1493354
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
