Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,513,641 |
G→A |
29.0% |
L80L (CTG→TTG) |
ubiX ← |
3‑octaprenyl‑4‑hydroxybenzoate carboxy‑lyase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,513,641 | 0 | G | A | 29.0%
| 113.9
/ 49.7
| 110 | L80L (CTG→TTG) | ubiX | 3‑octaprenyl‑4‑hydroxybenzoate carboxy‑lyase |
| Reads supporting (aligned to +/- strand): ref base G (26/52); new base A (15/17); total (41/69) |
| Fisher's exact test for biased strand distribution p-value = 1.99e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01 |
Only 100 of 110 total aligned reads displayed.
CCATCAGTATAGCTATGGACAATGCCGGAAAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAGCGTCTGGAAAGAACCGGAAGAGATGCTGGCGGCAATATCGCGCGCATCGTGCGTGACATCGGCT > minE/1513574‑1513705
|
ccATCAGTATAGCTATGGACAATGCCGGAAAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAGc < 1:1421418/69‑1 (MQ=255)
ccATCAGTATAGCTATGGACAATGCCGGAAAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAGc < 1:2991838/69‑1 (MQ=255)
aTCAGTATAGCTATGGACAATGCCGGACAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAACGt < 1:1627560/69‑1 (MQ=255)
aTCAGTATAGCTATGGACAATGCCGGACAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAACGt < 1:203301/69‑1 (MQ=255)
aTCAGTATAGCTATGGACAATGCCGGACAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAACGt < 1:391275/69‑1 (MQ=255)
aTCAGTATAGCTATGGACAATGCCGGACAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAACGt < 1:373813/69‑1 (MQ=255)
aTCAGTATAGCTATGGACAATGCCGGACAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAACGt < 1:359905/69‑1 (MQ=255)
aTCAGTATAGCTATGGACAATGCCGGACAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAACGt < 1:2059725/69‑1 (MQ=255)
aTCAGTATAGCTATGGACAATGCCGGACAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAACGt < 1:2272120/69‑1 (MQ=255)
aTCAGTATAGCTATGGACAATGCCGGACAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAACGt < 1:3133391/69‑1 (MQ=255)
tCAGTATAGCTATGGACAATGCCGGACAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAACGt < 1:2131682/68‑1 (MQ=255)
tCAGTATAGCTATGGACAATGCCGGACAGGGTTATGATTGAACAGGGTAAAATCACCATCCCCAACGt < 1:2920938/68‑1 (MQ=255)
aGTATAGCTATGGACAATGCCGGAAAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAGCGTCt > 1:2026013/1‑68 (MQ=255)
aGTATAGCTATGGACAATGCCGGAAAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAGCGTCt > 1:3279795/1‑68 (MQ=255)
aGTATAGCTATGGACAATGCCGGAAAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAGCGTCt > 1:2280/1‑68 (MQ=255)
aGTATAGCTATGGACAATGCCGGAAAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAGCGTCt > 1:172669/1‑68 (MQ=255)
taGCTGTGGACAATGCCGGACAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAACGTCTGGaa > 1:1649911/1‑68 (MQ=255)
taGCTATGGACAATGCCGTACAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAACGTCTGGaa > 1:1090709/1‑68 (MQ=255)
taGCTATGGACAATGCCGGACAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAACGTCTGGaa > 1:231500/1‑68 (MQ=255)
taGCTATGGACAATGCCGGACAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAACGTCTGGaa > 1:2249111/1‑68 (MQ=255)
taGCTATGGACAATGCCGGACAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAACGTCTGGaa > 1:1985818/1‑68 (MQ=255)
taGCTATGGACAATGCCGGACAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAACGTCTGGaa > 1:2634726/1‑68 (MQ=255)
taGCTATGGACAATGCCGGACAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAACGTCTGGaa > 1:1446463/1‑68 (MQ=255)
taGCTATGGACAATGCCGGACAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAACGTCTGGaa > 1:2051487/1‑68 (MQ=255)
taGCTATGGACAATGCCGGACAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAACGTCTGGaa > 1:3254714/1‑68 (MQ=255)
taGCTATGGACAATGCCGGACAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAACGTCTGGaa > 1:221358/1‑68 (MQ=255)
taGCTATGGACAATGCCGGACAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAACGTCTGGaa > 1:1059742/1‑68 (MQ=255)
taGCTATGGACAATGCCGGACAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAACGTCTGGa > 1:1614697/1‑67 (MQ=255)
taGCTATGGACAATGCCGGACAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAACGTCTGGa > 1:1502128/1‑67 (MQ=255)
taGCTATGGACAATGCCGGACAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAACGTCTGGa > 1:1288362/1‑67 (MQ=255)
gatATGGACAATGCCGGAAAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAGCGTCTGGAAAGa > 1:2304641/3‑69 (MQ=255)
gCTATGGACAATGCCGGAAAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAGCGTCTGGAAAg < 1:2191377/68‑1 (MQ=255)
gCTATGGACAATGCCGGAAAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAGCGTCTGGAAAg < 1:1950340/68‑1 (MQ=255)
gCTATGGACAATGCCGGAAAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAGCGTCTGGAAAg < 1:2633444/68‑1 (MQ=255)
gCTATGGACAATGCCGGAAAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAGCGTCTGGAAAg < 1:1726828/68‑1 (MQ=255)
gCTATGGACAATGCCGGAAAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAGCGTCTGGAAAg < 1:3076012/68‑1 (MQ=255)
gCTATGGACAATGCCGGAAAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAGCGTCTGGAAAg < 1:1012754/68‑1 (MQ=255)
gCTATGGACAATGCCGGAAAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAGCGTCTGGAAAGa > 1:2378911/1‑69 (MQ=255)
gCTATGGACAATGCCGGAAAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAGCGTCTGGAAAGa > 1:1658271/1‑69 (MQ=255)
gCTATGGACAATGCCGGAAAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAGCGTCTGGAAAGa > 1:1441631/1‑69 (MQ=255)
gCTATGGACAATGCCGGAAAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAGCGTCTGGAAAGa > 1:3425001/1‑69 (MQ=255)
gCTATGGACAATGCCGGAAAGGGTTTTGATTGAACAGGGTAAAATCACAATCCCCAGCGTCTGGAAAg < 1:1040317/68‑1 (MQ=255)
gACAATGCCGGAAAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAGCGTCTGGAAAg > 1:2088917/1‑62 (MQ=255)
gACAATGCCGGAAAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAGCGTCTGGAAAGAAcc > 1:2164679/1‑66 (MQ=255)
gACAATGCCGGAAAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAGCGTCTGGAAAGAAcc > 1:542232/1‑66 (MQ=255)
gACAATGCCGGAAAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAGCGTCTGGAAAGAAcc > 1:1785766/1‑66 (MQ=255)
gACAATGCCGGAAAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAGCGTCTGGAAAGAAcc > 1:2595631/1‑66 (MQ=255)
gACAATGCCGGAAAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAGCGTCTGGAAAGAAcc > 1:1105780/1‑66 (MQ=255)
gACAATGCCGGAAAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAGCGTCTGGAAAGAAcc > 1:1355533/1‑66 (MQ=255)
gACAATGCCGGAAAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAGCGTCTGGAAAGAAcc > 1:3045857/1‑66 (MQ=255)
aaTGCCGGAAAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAGCGTCTGGAAAGAACCGGAaga < 1:3299660/69‑1 (MQ=255)
aaTGCCGGAAAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAGCGTCTGGAAAGAACCGGAaga < 1:2577630/69‑1 (MQ=255)
aaTGCCGGAAAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAGCGTCTGGAAAGAACCGGAaga < 1:1349378/69‑1 (MQ=255)
aaTGCCGGAAAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAGCGTCTGGAAAGAACCGGAaga < 1:2775353/69‑1 (MQ=255)
aaTGCCGGAAAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAGCGTCTGGAAAGAACCGGAaga < 1:404864/69‑1 (MQ=255)
gCCGGAAAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAGCTTCTGGAAAGAACCGGAAGAGAt < 1:1129244/69‑1 (MQ=255)
gCCGGAAAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAGCGTCTGGAAAGAACCGGAAGAGAt < 1:3038980/69‑1 (MQ=255)
gCCGGAAAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAGCGTCTGGAAAGAACCGGAAGAGAt < 1:3137907/69‑1 (MQ=255)
gCCGGAAAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAGCGTCTGGAAAGAACCGGAAGAGAt < 1:1337440/69‑1 (MQ=255)
gCCGGAAAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAGCGTCTGGAAAGAACCGGAAGAGAt < 1:1993897/69‑1 (MQ=255)
gCCGGAAAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAGCGTCTGGAAAGAACCGGAAGAGAt < 1:1506435/69‑1 (MQ=255)
gCCGGAAAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAGCGTCTGGAAAGAACCGGAAGAGAt < 1:2488692/69‑1 (MQ=255)
ggAAAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAGCGTCTGGAAAGAACCGGAAGAGATGCt < 1:682057/69‑1 (MQ=255)
ggAAAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAGCGTCTGGAAAGAACCGGAAGAGATGCt < 1:1430321/69‑1 (MQ=255)
ggAAAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAGCGTCTGGAAAGAACCGGAAGAGATGCt < 1:423617/69‑1 (MQ=255)
ggTTTTGATTGAACAGGGTAAAATCACCATCCCCAGCGTCTGGAAAGAACCGGAAGAGATGCTggcggc < 1:1353928/69‑1 (MQ=255)
ttttGATTGAACAGGGTAAAATCACCATCCCCAGCGTCTGGAAAGAACCGGAAGAGAt < 1:3334732/58‑1 (MQ=255)
ttgattgaACAGGGTAAAATCACCATCCCCAGCGTCTGGAAAGAACCGGAAGAGATGCTGGCGGCAat > 1:695429/1‑68 (MQ=255)
ttgattgaACAGGGTAAAATCACCATCCCCAACGt < 1:1856922/35‑1 (MQ=255)
tgaACAGGGTAAAATCACCATCCCCAGCGTCTGGAAAGAACCGGAAGAGATGCTGGCGGCAATATcgcg > 1:2498917/1‑69 (MQ=255)
tgaACAGGGTAAAATCACCATCCCCAGCGTCTGGAAAGAACCGGAAGAGATGCTGGCGGCAATATcgcg > 1:819904/1‑69 (MQ=255)
cAGGGTAAAATCACCATCCCCAGCGTCTGGAAAGAACCGGAAGAGATGCTggcggc < 1:342500/56‑1 (MQ=255)
cAGGGTAAAATCACCATCCCCAGCGTCTGGAAAGAACCGGAAGAGATGCTggcggc < 1:283275/56‑1 (MQ=255)
cAGGGTAAAATCACCATCCCCAGCGTCTGGAAAGAACCGGAAGAGATGCTggcggc < 1:893563/56‑1 (MQ=255)
ggTAAAATCACCATCCCCAGCGTCTGGAAAGAACCGGAAGAGATGCTGGCGGCAATATCGCGCGCATcg < 1:746105/69‑1 (MQ=255)
ggTAAAATCACCATCCCCAGCGTCTGGAAAGAACCGGAAGAGATGCTGGCGGCAATATCGCGCGCATcg < 1:907101/69‑1 (MQ=255)
ggTAAAATCACCATCCCCAGCGTCTGGAAAGAACCGGAAGAGATGCTGGCGGCAATATCGCGCGCATcg < 1:186717/69‑1 (MQ=255)
ggTAAAATCACCATCCCCAGCGTCTGGAAAGAACCGGAAGAGATGCTGGCGGCAATATCGCGCGCATcg < 1:2936477/69‑1 (MQ=255)
ggTAAAATCACCATCCCCAGCGTCTGGAAAGAACCGGAAGAGATGCTGGCGGCAATATCGCGCGCATcg < 1:2671915/69‑1 (MQ=255)
gTAAAATCACCATCCCCAGCGTCTTGAAAGAACCGGAAGAGAt < 1:546498/43‑1 (MQ=255)
gTAAAATCACCATCCCCAGCGTCTGGAAAGAACCGGAaga < 1:3278017/40‑1 (MQ=255)
gTAAAATCACCATCCCCAGCGTCTGGAAAGAACCGGAaga < 1:1991408/40‑1 (MQ=255)
gTAAAATCACCATCCCCAGCGTCTGGAAAGAACCGGAaga < 1:475397/40‑1 (MQ=255)
gTAAAATCACCATCCCCAGCGTCTGGAAAGAACCGGAaga < 1:2744915/40‑1 (MQ=255)
gTAAAATCACCATCCCCAGCGTCTGGAAAGAACCGGAaga < 1:2842626/40‑1 (MQ=255)
gTAAAATCACCATCCCCAGCGTCTGGAAAGAACCGGAaga < 1:364786/40‑1 (MQ=255)
gTAAAATCACCATCCCCAGCGTCTGGAAAGAACCGGAaga < 1:3248210/40‑1 (MQ=255)
cccAGCGTCTGGAAAGAACCGGAAGAGATGCTGGCGGCAATATCGCGCGCATCGTGCGTGACATCGGCt < 1:1096944/69‑1 (MQ=255)
cccAGCGTCTGGAAAGAACCGGAAGAGATGCTGGCGGCAATATCGCGCGCATCGTGCGTGACATCGGCt > 1:2879787/1‑69 (MQ=255)
cccAGCGTCTGGAAAGAACCGGAAGAGATGCTGGCGGCAATATCGCGCGCATCGTGCGTGACATCGGCt < 1:1176502/69‑1 (MQ=255)
cccAGCGTCTGGAAAGAACCGGAAGAGATGCTGGCGGCAATATCGCGCGCATCGTGCGTGACATCGGCt < 1:637240/69‑1 (MQ=255)
cccAGCGTCTGGAAAGAACCGGAAGAGATGCTGGCGGCAATATCGCGCGCATCGTGCGTGACATCGGCt > 1:694729/1‑69 (MQ=255)
cccAGCGTCTGGAAAGAACCGGAAGAGATGCTGGCGGCAATATCGCGCGCATCGTGCGTGACATCGGCt < 1:183540/69‑1 (MQ=255)
cccAGCGTCTGGAAAGAACCGGAAGAGATGCTGGCGGCAATATCGCGCGCATCGTGCGTGACATCGGCt < 1:1211195/69‑1 (MQ=255)
cccAGCGTCTGGAAAGAACCGGAAGAGATGCTGGCGGCAATATCGCGCGCATCGTGCGTGACATCGGCt < 1:2048627/69‑1 (MQ=255)
cccAACGTCTGGAAAGAACCGGAAGAAATGCTGGCAGCAATATCGCGCGCATCGTGTGTCACATCAGCt < 1:288011/69‑1 (MQ=255)
cccAACGTCTGGAAAGAACCGGAAGAAATGCTGGCAGCAATATCGCGCGCATCGTGTGTCACATCAGCt < 1:265750/69‑1 (MQ=255)
cccAACGTCTGGAAAGAACCGGAAGAAATGCTGGCAGCAATATCGCGCGCATCGTGTGTCACATCAGCt < 1:2905959/69‑1 (MQ=255)
cccAACGTCTGGAAAGAACCGGAAGAAATGCTGGCAGCAATATCGCGCGCATCGTGTGTCACATCAGCt < 1:2644384/69‑1 (MQ=255)
cccAACGTCTGGAAAGAACCGGAAGAAATGCTGGCAGCAATATCGCGCGCATCGTGTGTCACATCAGCt < 1:2153445/69‑1 (MQ=255)
|
CCATCAGTATAGCTATGGACAATGCCGGAAAGGGTTTTGATTGAACAGGGTAAAATCACCATCCCCAGCGTCTGGAAAGAACCGGAAGAGATGCTGGCGGCAATATCGCGCGCATCGTGCGTGACATCGGCT > minE/1513574‑1513705
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
