Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,514,486 |
A→G |
31.3% |
F335F (TTT→TTC) |
purF ← |
amidophosphoribosyltransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,514,486 | 0 | A | G | 31.3%
| 55.7
/ 32.0
| 67 | F335F (TTT→TTC) | purF | amidophosphoribosyltransferase |
| Reads supporting (aligned to +/- strand): ref base A (22/24); new base G (13/8); total (35/32) |
| Fisher's exact test for biased strand distribution p-value = 3.07e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.32e-01 |
CTCGGCGCGGTTGGCATTCAGTTTACGGCGCACGGACTTACGACGCAGCTGCTGGCCCGGCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCA > minE/1514420‑1514552
|
cTCGGCGCGGTTGGCATTCAGTTTACGGCGCACGGACTTACGACGCAGCTGCTGGCCCGGCATGATaaa < 1:558108/69‑1 (MQ=255)
cTCGGCGCGGTTGGCATTCAGTTTACGGCGCACGGACTTACGACGCAGCTGCTGGCCCGGCATGATaaa < 1:3122923/69‑1 (MQ=255)
cTCGGCGCGGTTGGCATTCAGTTTACGGCGCACGGACTTACGACGCAGCTGCTGGCCCGGCATGATaaa < 1:2212129/69‑1 (MQ=255)
cTCGGCGCGGTTGGCATTCAGTTTACGGCGCACGGACTTACGACGCAGCTGCTGGCCCGGCATGATaaa < 1:2401107/69‑1 (MQ=255)
cTCGGCGCGGTTGGCATTCAGTTTACGGCGCACGGACTTACGACGCAGCTGCTGGCCCGGCATGATaaa < 1:2694593/69‑1 (MQ=255)
cTCGGCGCGGTTGGCATTCAGTTTACGGCGCACGGACTTACGACGCAGCTGCTGGCCCGGCATGATaaa < 1:2659569/69‑1 (MQ=255)
cTCGGCGCGGTTGGCATTCAGTTTACGGCGCACGGACTTACGACGCAGCTGCTGGCCCGGCATGATaaa < 1:2558090/69‑1 (MQ=255)
cTCGGCGCGGTTGGCATTCAGTTTACGGCGCACGGACTTACGACGCAGCTGCTGGCCAGGCATGATaaa < 1:1929870/69‑1 (MQ=255)
cGGCGCGGTTGGCATTCAGTTTACGGCGCACGGACTTACGACGCAGCTGCTGGCCCGGCATGATAAAgg < 1:2768743/69‑1 (MQ=255)
gcgcGGTTGGCATTCAGTTTACGGCGCAGGGACTTACGACGCAGCTGCTGGCCCGGCATGATaaa < 1:1156471/65‑1 (MQ=255)
tGGCATTCAGTTTACGGCGCACGGACTTACGACGCAGCTGCTGGCCCGGCATGATAAAGGTGCGGCCaa < 1:2597484/69‑1 (MQ=255)
tGGCATTCAGTTTACGGCGCACGGACTTACGACGCAGCTGCTGGCCCGGCATGATAAAGGTGCGGCCaa < 1:50630/69‑1 (MQ=255)
tGGCATTCAGTTTACGGCGCACGGACTTACGACGCAGCTGCTGGCCCGGCATGATAAAGGTGCGGCCaa < 1:1467010/69‑1 (MQ=255)
cAGTTTGCGGCGCACGGACTTACGACGCAGCTGCTGGCCCGGCATGATAAAGGTGCGGCCAACATAGCg > 1:439275/1‑69 (MQ=255)
cAGTTTACGGCGCACGGACTTACGACGCAGCTGCTGGCCCGGCATGATAAGGGTGCGGCCAACATAGCg > 1:795112/1‑69 (MQ=255)
cAGTTTACGGCGCACGGACTTACGACGCAGCTGCTGGCCCGGCATGATAAAGGTGCGGCCAACATAGCg > 1:3405561/1‑69 (MQ=255)
cAGTTTACGGCGCACGGACTTACGACGCAGCTGCTGGCCCGGCATGATAAAGGTGCGGCCAACATAGCg > 1:473939/1‑69 (MQ=255)
cAGTTTACGGCGCACGGACTTACGACGCAGCTGCTGGCCCGGCATGATAAAGGTGCGGCCAACATAGCg > 1:2981604/1‑69 (MQ=255)
cAGTTTACGGCGCACGGACTTACGACGCAGCTGCTGGCCCGGCATGATAAAGGTGCGGCCAACATAGCg > 1:3050994/1‑69 (MQ=255)
cAGTTTACGGCGCACGGACTTACGACGCAGCTGCTGGCCCGGCATGATAAAGGTGCGGCCAACATAGCg > 1:513813/1‑69 (MQ=255)
cAGTTTACGGCGCACGGACTTACGACGCAGCTGCTGGCCCGGCATGATAAAGGTGCGGCCAACATAGCg > 1:2058423/1‑69 (MQ=255)
cAGTTTACGGCGCACGGACTTACGACGCAGCTGCTGGCCCGGCATGATAAAGGTGCGGCCAACATAGCg > 1:37423/1‑69 (MQ=255)
cAGTTTACGGCGCACGGACTTACGACGCAGCTGCTGGCCCGGCATGATAAAGGTGCGGCCAACATAGCg > 1:1005773/1‑69 (MQ=255)
cAGTTTACGGCGCACGGACTTACGACGCAGCTGCTGGCCCGGCATGATAAAGGTGCGGCCAACATAGCg > 1:849744/1‑69 (MQ=255)
cAGTTTACGGCGCACGGACTTACGACGCAGCTGCTGGCCCGGCATGATAAAGGTGCGGCCAACATAGCg > 1:723794/1‑69 (MQ=255)
gTTTACGGCGCACGGACTTACGACGCAGCTGCTGGCCCGGCATGATAAAGGTGCGGCCAACATAGCgg < 1:3289844/68‑1 (MQ=255)
tACGGCGCACGGACTTACGACGCAGCTGCTGGCCCGGCATGATAAAGGTGCGGCCAACATAGCGGttt > 1:1085613/1‑68 (MQ=255)
tACGGCGCACGGACTTACGACGCAGCTGCTGGCCCGGCATGATAAAGGTGCGGCCAACATAGCGGttt > 1:2368440/1‑68 (MQ=255)
tACGGCGCACGGACTTACGACGCAGCTGCTGGCCCGGCATGATAAAGGTGCGGCCAACATAGCGGttt > 1:1153366/1‑68 (MQ=255)
tACGGCGCACGGACTTACGACGCAGCTGCTGGCCCGGCATGATAAAGGTGCGGCCAACATAGCGGttt > 1:2910099/1‑68 (MQ=255)
tACGGCGCACGGACTTACGACGCAGCTGCTGGCCCGGCATGATAAAGGTGCGGCCAACATAGCGGttt > 1:2696448/1‑68 (MQ=255)
tACGGCGCACGGACTTACGACGCAGCTGCTGGCCCGGCATGATAAAGGTGCGGCCAACATAGCGGttt > 1:747487/1‑68 (MQ=255)
cGGACTTACGACGCAGCTGCTGGCCCGGCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGc < 1:215394/69‑1 (MQ=255)
cGGACTTACGACGCAGCTGCTGGCCCGGCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGc < 1:3084279/69‑1 (MQ=255)
cGGACTTACGACGCAGCTGCTGACCCGGCATGATGAAGGTGCGGCCAACATAACGGTTTTTAACGAAg > 1:2363968/1‑68 (MQ=255)
cGGACTTACGACGCAGCTGCTGACCCGGCATGATGAAGGTGCGGCCAACATAACGGTTTTTAACGAAGc > 1:571533/1‑69 (MQ=255)
cGGACTTACGACGCAGCTGCTGACCCGGCATGATGAAGGTGCGGCCAACATAACGGTTTTTAACGAAGc > 1:1122243/1‑69 (MQ=255)
cGGACTTACGACGCAGCTGCTGACCCGGCATGATGAAGGTGCGGCCAACATAACGGTTTTTAACGAAGc > 1:2610367/1‑69 (MQ=255)
cGGACTTACGACGCAGCTGCTGACCCGGCATGATGAAGGTGCGGCCAACATAACGGTTTTTAACGAAGc > 1:258230/1‑69 (MQ=255)
cGGACTTACGACGCAGCTGCTGACCCGGCATGATGAAGGTGCGGCCAACATAACGGTTTTTAACGAAGc > 1:2554709/1‑69 (MQ=255)
cGGACTTACGACGCAGCTGCTGACCCGGCATGATGAAGGTGCGGCCAACATAACGGTTTTTAACGAAGc > 1:2152108/1‑69 (MQ=255)
cGGACTTACGACGCAGCTGCTGACCCGGCATGATGAAGGTGCGGCCAACATAACGGTTTTTAACGAAGc > 1:1873110/1‑69 (MQ=255)
cGGACTTACGACGCAGCTGCTGACCCGGCATGATGAAGGTGCGGCCAACATAACGGTTTTTAACGAAGc > 1:2383278/1‑69 (MQ=255)
cGGACTTACGACGCAGCTGCTGACCCGGCATGATGAAGGTGCGGCCAACATAACGGTTTTTAACGAAGc > 1:2299822/1‑69 (MQ=255)
cGGACTTACGACGCAGCTGCTGACCCGGCATGATGAAGGTGCGGCCAACATAACGGTTTTTAACGAAGc > 1:703878/1‑69 (MQ=255)
cGGACTTACGACGCAGCTGCTGACCCGGCATGATGAAGGTGCGGCCAACATAACGGTTTTTAACGAAGc > 1:1783858/1‑69 (MQ=255)
cGGACTTACGACGCAGCTGCTGACCCGGCATGATGAAGGTGCGGCCAAAATAACGGTTTTTAACGAAGc > 1:1194744/1‑69 (MQ=255)
ggACTTACGACGCAGCTGCTGGCCCGGCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGc < 1:2445857/68‑1 (MQ=255)
gacgCAGCTGCTGACCCGGCATGATGAAGGTGCGGCCAACATAACGGTTTTTAACGAAGCCCTGGCgg < 1:1568739/68‑1 (MQ=255)
gacgCAGCTGCTGACCCGGCATGATGAAGGTGCGGCCAACATAACGGTTTTTAACGAAGCCCTGGCgg < 1:2018453/68‑1 (MQ=255)
gacgCAGCTGCTGACCCGGCATGATGAAGGTGCGGCCAACATAACGGTTTTTAACGAAGCCCTGGCgg < 1:39067/68‑1 (MQ=255)
gacgCAGCTGCTGACCCGGCATGATGAAGGTGCGGCCAACATAACGGTTTTTAACGAAGCCCTGGCgg < 1:3113635/68‑1 (MQ=255)
gacgCAGCTGCTGACCCGGCATGATGAAGGTGCGGCCAACATAACGGTTTTTAACGAAGCCCTGGCgg < 1:1629795/68‑1 (MQ=255)
gacgCAGCTGCTGACCCGGCATGATGAAGGTGCGGCCAACATAACGGTTTTTAACGAAGCCCTGGCgg < 1:2879268/68‑1 (MQ=255)
gacgCAGCTGCTGACCCGGCATGATGAAGGTGCGGCCAACATAACGGTTTTTAACGAAGCCCTGGCgg < 1:1158219/68‑1 (MQ=255)
gacgCAGCTGCTGACCCGGCATGATGAAGGTGCGGCCAACATAACGGTTTTTAACGAAGCCCTGGCgg < 1:758319/68‑1 (MQ=255)
ccGGCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAg < 1:862958/67‑1 (MQ=255)
ccGGCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAg < 1:1122308/67‑1 (MQ=255)
ccGGCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAg < 1:1138310/67‑1 (MQ=255)
ccGGCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAg < 1:1709569/67‑1 (MQ=255)
ccGGCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGaa < 1:3302288/69‑1 (MQ=255)
ccGGCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGaa < 1:1166213/69‑1 (MQ=255)
ccGGCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGaa < 1:2236930/69‑1 (MQ=255)
ccGGCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGaa < 1:2206109/69‑1 (MQ=255)
ccGGCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGaa < 1:1439484/69‑1 (MQ=255)
ccGGCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGaa < 1:607528/69‑1 (MQ=255)
ccGGCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGaa < 1:1736031/69‑1 (MQ=255)
tgCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATa < 1:1076379/68‑1 (MQ=255)
ggCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATa < 1:1949757/69‑1 (MQ=255)
ggCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATa < 1:1958111/69‑1 (MQ=255)
ggCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATa < 1:501968/69‑1 (MQ=255)
ggCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATa < 1:2423907/69‑1 (MQ=255)
aTGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATAc > 1:3243563/1‑67 (MQ=255)
aTAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCt > 1:650472/1‑40 (MQ=255)
aTAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCa > 1:3400126/1‑69 (MQ=255)
aTAAAGGTCCCGCCAACATAGCGGTTTTTAACGAAGCCCTcgcg > 1:3423979/1‑44 (MQ=255)
|
CTCGGCGCGGTTGGCATTCAGTTTACGGCGCACGGACTTACGACGCAGCTGCTGGCCCGGCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCA > minE/1514420‑1514552
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
