Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,565,578 |
G→T |
29.7% |
G156G (GGC→GGA) |
glk ← |
glucokinase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,565,578 | 0 | G | T | 29.7%
| 115.5
/ 46.5
| 85 | G156G (GGC→GGA) | glk | glucokinase |
| Reads supporting (aligned to +/- strand): ref base G (16/43); new base T (5/20); total (21/64) |
| Fisher's exact test for biased strand distribution p-value = 5.89e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.24e-01 |
TTTCGAGGATAATGGCCTCTTCTTCACTATTCGGCGCAAAATCAACGTGACCGCCTTCGCCTGGCAAGCTTACCCAACGCTTATCGACATGGACCAGATGCGCAACCCCAAGCCCCGTTCCGGCAC > minE/1565520‑1565645
|
tttCGAGGATAATGGCCTCTTCTTCCCTATTCGGCGCAAAATCAACGTGACCGCCTTCGCCTGGCAAg > 1:2708333/1‑68 (MQ=255)
tttCGAGGATAATGGCCTCTTCTTCACTATTCGGCGCAAAATCAACGTGACCGCCTTCGCCTGGCAAg > 1:2347506/1‑68 (MQ=255)
tttCGAGGATAATGGCCTCTTCTTCACTATTCGGCGCAAAATCAACGTGACCGCCTTCGCCTGGCAAg > 1:382933/1‑68 (MQ=255)
tttCGAGGATAATGGCCTCTTCTTCACTATTCGGCGCAAAATCAACGTGACCGCCTTCGCCTGGCAAg > 1:2399546/1‑68 (MQ=255)
tttCGAGGATAATGGCCTCTTCTTCACTATTCGGCGCAAAATCAACGTGACCGCCTTCGCCTGGCAAg > 1:1365057/1‑68 (MQ=255)
tttCGAGGATAATGGCCTCTTCTTCACTATTCGGCGCAAAATCAACGTGACCGCCTTCGCCTGGCAAg > 1:1686136/1‑68 (MQ=255)
ggATAATGCCCTCTTCTTCACTATTCGGCGCAAAATCAACGTGACCGCCTTCTCCTGGCAAGCTTAccc < 1:1121353/69‑1 (MQ=255)
ggATAATGCCCTCTTCTTCACTATTCGGCGCAAAATCAACGTGACCGCCTTCTCCTGGCAAGCTTAccc < 1:3417746/69‑1 (MQ=255)
ggATAATGCCCTCTTCTTCACTATTCGGCGCAAAATCAACGTGACCGCCTTCTCCTGGCAAGCTTAccc < 1:1280265/69‑1 (MQ=255)
ggATAATGCCCTCTTCTTCACTATTCGGCGCAAAATCAACGTGACCGCCTTCTCCTGGCAAGCTTAccc < 1:817197/69‑1 (MQ=255)
tAATGGCCTCTTCTTCACTATTCGGCGCAAAATCAACGTGACCGCCTTCGCCTGGCAAGCTTACCCAAc > 1:2145787/1‑69 (MQ=255)
tAATGGCCTCTTCTTCACTATTCGGCGCAAAATCAACGTGACCGCCTTCGCCTGGCAAGCTTACCCAAc > 1:2402732/1‑69 (MQ=255)
aaTGGCCTCTTCTTCACTATTCGGCGCAAAATCAACGTGACCGCCTTCGCCTGGCAAGCTTACCCAACg > 1:1681805/1‑69 (MQ=255)
aaTGGCCTCTTCTTCACTATTCGGCGCAAAATCAACGTGACCGCCTTCGCCTGGCAAGCTTACCCAACg > 1:1488242/1‑69 (MQ=255)
aaTGGCCTCTTCTTCACTATTCGGCGCAAAATCAACGTGACCGCCTTCGCCTGGCAAGCTTACCCAACg > 1:2725399/1‑69 (MQ=255)
aaTGGCCTCTTCTTCACTATTCGGCGCAAAATCAACGTGACCGCCTTCGCCTGGCAAGCTTACCCAACg > 1:351892/1‑69 (MQ=255)
aaTGGCCTCTTCTTCACTATTCGGCGCAAAATCAACGTGACCGCCTTCGCCTGGCAAGCTTACCCAACg > 1:2393294/1‑69 (MQ=255)
tGGCCTCTTCTTCACTATTCGGCGCAAAATCAACGTGACCGCCTTCGCCTGGCAAGCTTACCCAACGCt > 1:3362642/1‑69 (MQ=255)
ctctTCTTCACTATTCGGCGCAAAATCAACGTGACCGCCTTCGCCTGGCAAGCTTACCCAACGCTTATc > 1:1290630/1‑69 (MQ=255)
cttcttCACTATTCGGCGCAAAATCAACGTGACCGCCTTCGCCTGGCAAGCTTACCCAACGCTTATCGa < 1:3001253/69‑1 (MQ=255)
cttcttCACTATTCGGCGCAAAATCAACGTGACCGCCTTCGCCTGGCAAGCTTACCCAACGCTTATCGa < 1:1586009/69‑1 (MQ=255)
cttcttCACTATTCGGCGCAAAATCAACGTGACCGCCTTCGCCTGGCAAGCTTACCCAACGCTTATCGa < 1:1399704/69‑1 (MQ=255)
cttcttCACTATTCGGCGCAAAATCAACGTGACCGCCTTCGCCTGGCAAGCTTACCCAACGCTTATCGa < 1:1713041/69‑1 (MQ=255)
cttcttCACTATTCGGCGCAAAATCAACGTGACCGCCTTCGCCTGGCAAGCTTACCCAACGCTTATCGa < 1:820196/69‑1 (MQ=255)
cttcttCACTATTCGGCGCAAAATCAACGTGACCGCCTTCGCCTGGCAAGCTTACCCAACGCTTATCGa < 1:3056539/69‑1 (MQ=255)
cttcttCACTATTCGGCGCAAAATCAACGTGACCGCCTTCGCCTGGCAAGCTTACCCAACGCTTATCGa < 1:596853/69‑1 (MQ=255)
tcACTATTCGGCGCAAAATCAACGTGACCGCCTTCGCCTGGCAAGCTTACCCAACGCTTATCGACATg < 1:2507476/68‑1 (MQ=255)
tcACTATTCGGCGCAAAATCAACGTGACCGCCTTCGCCTGGCAAGCTTACCCAACGCTTATCGACATg < 1:2883524/68‑1 (MQ=255)
tcACTATTCGGCGCAAAATCAACGTGACCGCCTTCGCCTGGCAAGCTTACCCAACGCTTATCGACATg < 1:2673303/68‑1 (MQ=255)
tcACTATTCGGCGCAAAATCAACGTGACCGCCTTCGCCTGGCAAGCTTACCCAACGCTTATCGACATg < 1:2689727/68‑1 (MQ=255)
tcACTATTCGGCGCAAAATCAACGTGACCGCCTTCGCCTGGCAAGCTTACCCAACGCTTATCGACATg < 1:274636/68‑1 (MQ=255)
tcACTATTCGGCGCAAAATCAACGTGACCGCCTTCGCCTGGCAAGCTTACCCAACGCTTATCGACATg < 1:2151774/68‑1 (MQ=255)
tcACTATTCGGCGCAAAATCAACGTGACCGCCTTCGCCTGGCAAGCTTACCCAACGCTTATCGACATg < 1:3165762/68‑1 (MQ=255)
tcACTATTCGGCGCAAAATCAACGTGACCGCCTTCGCCTGGCAAGCTTACCCAACGCTTATCGACATg < 1:3273396/68‑1 (MQ=255)
tcACTATTCGGCGCAAAATCAACGTGACCGCCTTCGCCTGGCAAGCTTACCCAACGCTTATCGACATg < 1:783320/68‑1 (MQ=255)
tcACTATTCGGCGCAAAATCAACGTGACCGCCTTCGCCTGGCAAGCTTACCCAACGCTTATCGACATg < 1:102287/68‑1 (MQ=255)
tcACTATTCGGCGCAAAATCAACGTGACCGCCTTCGCCTGGCAAGCTTACCCAACGCTTATCGACATg < 1:1153941/68‑1 (MQ=255)
tcACTATTCGGCGCAAAATCAACGTGACCGCCTTCGCCTGGCAAGCTTACCCAACGCTTATCGACATg < 1:1547865/68‑1 (MQ=255)
tcACTATTCGGCGCAAAATCAACGTGACCGCCTTCGCCTGGCAAGCTTACCCAACGCTTATCGACATg < 1:78777/68‑1 (MQ=255)
tcACTATTCGGCGCAAAATCAACGTGACCGCCTTCGCCTGGCAAGCTTACCCAACGCTTATCGACATg < 1:740655/68‑1 (MQ=255)
tcACTATTCGGCGCAAAATCAACGTGACCGCCTTCGCCTGGCAAGCTTACCCAACGCTTATCGACATg < 1:464794/68‑1 (MQ=255)
tcACTATTCGGCGCAAAATCAACGTGACCGCCTTCGCCTGGCAAGCTTACCCAACGCTTATCGACATg < 1:2216052/68‑1 (MQ=255)
tcACTATTCGGCGCAAAATCAACGTGACCGCCTTCGCCTGGCAAGCTTACCCAACGCTTATCGACATg < 1:451561/68‑1 (MQ=255)
cACTATTCGGCGCAAAATCAACGTGACCGCCTTCGCCTGGCAAGCTTACCCAACGCTTATCGACATg < 1:1079670/67‑1 (MQ=255)
cTATTCGGCGCAAAATCAACGTGACCGCCTTCGCCTGGCAAGCTTACCCAACGCTTATCGACATGGAc < 1:1731413/68‑1 (MQ=255)
aTTCGGCGCAAAATCAACGTGACCGCCTTCGCCTGGCAAGCTTACCCAACGCTTATCGACATGGAcct < 1:1791562/68‑2 (MQ=255)
aTTCGGCGCAAAATCAACGTGACCGCCTTCGCCTGGCAAGCTTACCCAACGCTTATCGACATGGACCa < 1:585423/68‑1 (MQ=255)
aTTCGGCGCAAAATCAACGTGACCGCCTTCGCCTGGCAAGCTTACCCAACGCTTATCGACATGGACCa < 1:300854/68‑1 (MQ=255)
aTTCGGCGCAAAATCAACGTGACCGCCTTCGCCTGGCAAGCTTACCCAACGCTTATCGACATGGACCa < 1:2311838/68‑1 (MQ=255)
aTTCGGCGCAAAATCAACGTGACCGCCTTCGCCTGGCAAGCTTACCCAACGCTTATCGACATGGACCa < 1:921503/68‑1 (MQ=255)
aTTCGGCGCAAAATCAACGTGACCGCCTTCACCTGGCAAGCTTACCCAACGCTTATCGACATGGACCa < 1:2902152/68‑1 (MQ=255)
ttCGGCGCAAAATCAACGTGACCGCCTTCGCCTTGCAAGCTTACCCAACGCTTATCGAc < 1:3281707/59‑1 (MQ=255)
ttCGGCGCAAAATCAACGTGACCGCCTTCGCCTGGCAAGCTTACCCAACGCTTATCGAc < 1:547490/59‑1 (MQ=255)
ttCGGCGCAAAATCAACGTGACCGCCTTCGCCTGGCAAGCTTACCCAACGCTTATCGAc < 1:1056818/59‑1 (MQ=255)
ttCGGCGCAAAATCAACGTGACCGCCTTCGCCTGGCAAGCTTACCCAACGCTTATCGAc < 1:395545/59‑1 (MQ=255)
ttCGGCGCAAAATCAACGTGACCGCCTTCGCCTGGCAAGCTTACCCAACGCTTATCGAc < 1:411107/59‑1 (MQ=255)
ttCGGCGCAAAATCAACGTGACCGCCTTCGCCTGGCAAGCTTACCCAACGCTTATCGAc < 1:309047/59‑1 (MQ=255)
ttCGGCGCAAAATCAACGTGACCGCCTTCGCCTGGCAAGCTTACCCAACGCTTATCGAc < 1:2722719/59‑1 (MQ=255)
ttCGGCGCAAAATCAACGTGACCGCCTTCGCCTGGCAAGCTTACCCAACGCTTATCGAc < 1:1691974/59‑1 (MQ=255)
ttCGGCGCAAAATCAACGTGACCGCCTTCGCCTGGCAAGCTTACCCAACGCTTATCGAc < 1:1695964/59‑1 (MQ=255)
ttCGGCGCAAAATCAACGTGACCGCCTTCGCCTGGCAAGCTTACCCAACGCTTATCGAc < 1:1732245/59‑1 (MQ=255)
cGGCGCAAAATCAACGTGACCGCCTTCTCCTGGCAAGCTTACCCaa < 1:991205/46‑1 (MQ=255)
cGGCGCAAAATCAACGTGACCGCCTTCTCCTGGCAAGCTTACCCaa < 1:3092653/46‑1 (MQ=255)
cGGCGCAAAATCAACGTGACCGCCTTCTCCTGGCAAGCTTACCCaa < 1:872286/46‑1 (MQ=255)
cGGCGCAAAATCAACGTGACCGCCTTCTCCTGGCAAGCTTACCCaa < 1:1221936/46‑1 (MQ=255)
cGGCGCAAAATCAACGTGACCGCCTTCTCCTGGCAAGCTTACCCaa < 1:766758/46‑1 (MQ=255)
cGGCGCAAAATCAACGTGACCGCCTTCTCCTGGCAAGCTTACCCaa < 1:1786962/46‑1 (MQ=255)
cGGCGCAAAATCAACGTGACCGCCTTCTCCTGGCAAGCTTACCCaa < 1:676738/46‑1 (MQ=255)
cGGCGCAAAATCAACGTGACCGCCTTCTCCTGGCAAGCTTACCCaa < 1:1885774/46‑1 (MQ=255)
cGGCGCAAAATCAACGTGACCGCCTTCTCCTGGCAAGCTTACCCaa < 1:589903/46‑1 (MQ=255)
cGGCGCAAAATCAACGTGACCGCCTTCTCCTGGCAAGCTTACCCaa < 1:588800/46‑1 (MQ=255)
cGGCGCAAAATCAACGTGACCGCCTTCTCCTGGCAAGCTTACCCaa < 1:1965318/46‑1 (MQ=255)
cGGCGCAAAATCAACGTGACCGCCTTCTCCTGGCAAGCTTACCCaa < 1:230755/46‑1 (MQ=255)
cGGCGCAAAATCAACGTGACCGCCTTCTCCTGGCAAGCTTACCCaa < 1:2316829/46‑1 (MQ=255)
cGGCGCAAAATCAACGTGACCGCCTTCTCCTGGCAAGCTTACCCaa < 1:2841620/46‑1 (MQ=255)
cGGCGCAAAATCAACGTGACCGCCTTCTCCTGGCAAGCTTACCCaa < 1:3404492/46‑1 (MQ=255)
cGGCGCAAAATCAACGTGACCGCCTTCTCCTGGCAAGCTTACCCaa < 1:3403242/46‑1 (MQ=255)
gcAAAATCAACGTGACCGCCTTCTCCTGGCAAGCTTACCCAACGCTTATCGACATGGACCAGATGCGCa > 1:1925955/1‑69 (MQ=255)
gcAAAATCAACGTGACCGCCTTCTCCTGGCAAGCTTACCCAACGCTTATCGACATGGACCAGATGCGCa > 1:632015/1‑69 (MQ=255)
gcAAAATCAACGTGACCGCCTTCTCCTGGCAAGCTTACCCAACGCTTATCGACATGGACCAGATGCGCa > 1:791056/1‑69 (MQ=255)
gcAAAATCAACGTGACCGCCTTCTCCTGGCAAGCTTACCCAACGCTTATCGACATGGACCAGATGCGCa > 1:2662761/1‑69 (MQ=255)
gcAAAATCAACGTGACCGCCTTCTCCTGGCAAGCTTACCCAACGCTTATCGACATGGACCAGATGCGCa > 1:1139821/1‑69 (MQ=255)
cAACGTGACCGCCTTCGCCTGGCAAGCTTACCCAACGCTTATCGACATg < 1:1957723/49‑1 (MQ=255)
ccGCCTTCGCCTGGCAAGCTTACCCAACGCTTATCGAc < 1:88249/38‑1 (MQ=255)
cGCCTGGCAAGCTTACCCAACGCTTATCGACATGGACCAGATGCGCAACCCCAAGCCCCGTTCCGGCAc > 1:2381690/1‑69 (MQ=255)
|
TTTCGAGGATAATGGCCTCTTCTTCACTATTCGGCGCAAAATCAACGTGACCGCCTTCGCCTGGCAAGCTTACCCAACGCTTATCGACATGGACCAGATGCGCAACCCCAAGCCCCGTTCCGGCAC > minE/1565520‑1565645
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
