Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,609,750 |
A→T |
28.5% |
V202V (GTT→GTA) |
eutC ← |
ethanolamine ammonia‑lyase, small subunit (light chain) |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,609,750 | 0 | A | T | 28.5%
| 79.5
/ 36.1
| 63 | V202V (GTT→GTA) | eutC | ethanolamine ammonia‑lyase, small subunit (light chain) |
| Reads supporting (aligned to +/- strand): ref base A (18/27); new base T (3/15); total (21/42) |
| Fisher's exact test for biased strand distribution p-value = 1.37e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01 |
ATCACTACTTTCGCGCCGAGGATCTCACCGATCTGATCTTCAATCTTCACGCGACCATAACGAACAAAGAATGGTGTACCGACTTTCAGCCCGGCCTGTTTCAGGCCCGCCATCAGCGGT > minE/1609688‑1609807
|
aTCACTACTTTCGCGCCGAGGATCTCACCGATCTGATCTTCAATCTTCACGCGACCATAACGAACAAAg < 1:884112/69‑1 (MQ=255)
aTCACTACTTTCGCGCCGAGGATCTCACCGATCTGATCTTCAATCTTCACGCGACCATAACGAACAAAg < 1:1039312/69‑1 (MQ=255)
aTCACTACTTTCGCGCCGAGGATCTCACCGATCTGATCTTCAATCTTCACGCGACCATAACGAACAAAg < 1:1061427/69‑1 (MQ=255)
aTCACTACTTTCGCGCCGAGGATCTCACCGATCTGATCTTCAATCTTCACGCGACCATAACGAACAAAg < 1:2936908/69‑1 (MQ=255)
aTCACTACTTTCGCGCCGAGGATCTCACCGATCTGATCTTCAATCTTCACGCGACCATAACGAACAAAg < 1:1360173/69‑1 (MQ=255)
actTTCGCGCCGAGGATCTCACCGATCTGATCTTCAATCTTCACGCGACCATAACGAACAAAg < 1:2216927/63‑1 (MQ=255)
actTTCGCGCCGAGGATCTCACCGATCTGATCTTCAATCTTCACGCGACCATAACGAACAAAg < 1:572778/63‑1 (MQ=255)
actTTCGCGCCGAGGATCTCACCGATCTGATCTTCAATCTTCACGCGACCATAACGAACAAAg < 1:2136849/63‑1 (MQ=255)
actTTCGCGCCGAGGATCTCACCGATCTGATCTTCAATCTTCACGCGACCATAACGAACAAAg > 1:3294398/1‑63 (MQ=255)
ctcACCGATCTGATCTTCAATCTTCACGCGACCATAACGAACAAAGAATGGTGTACCGACTTTCAGccc > 1:2634039/1‑69 (MQ=255)
ctcACCGATCTGATCTTCAATCTTCACGCGACCATAACGAACAAAGAATGGTGTACCGACTTTCAGccc > 1:2700588/1‑69 (MQ=255)
ctcACCGATCTGATCTTCAATCTTCACGCGACCATAACGAACAAAGAATGGTGTACCGACTTTCAGccc > 1:2027748/1‑69 (MQ=255)
ctcACCGATCTGATCTTCAATCTTCACGCGACCATAACGAACAAAGAATGGTGTACCGACTTTCAGccc > 1:1809663/1‑69 (MQ=255)
aCCGATCTGATCTTCAATCTTCACGCGACCATAACGAACAAAGAATGGTGTACCGACTTTCAGCCCGGc > 1:2479963/1‑69 (MQ=255)
aCCGATCTGATCTTCAATCTTCACGCGACCATAACGAACAAAGAATGGTGTACCGACTTTCAGCCCGGc > 1:2281939/1‑69 (MQ=255)
cGATCTGATCTTCAATCTTCACGCGACCATAACGAACAAAGAATGGTGTACCGACTTTCAGCCCGGCCt < 1:1544919/69‑1 (MQ=255)
cGATCTGATCTTCAATCTTCACGCGACCATAACGAACAAAGAATGGTGTACCGACTTTCAGCCCGGCCt < 1:1718466/69‑1 (MQ=255)
cGATCTGATCTTCAATCTTCACGCGACCATAACGAACAAAGAATGGTGTACCGACTTTCAGCCCGGCCt < 1:1492850/69‑1 (MQ=255)
cGATCTGATCTTCAATCTTCACGCGACCATAACGAACAAAGAATGGTGTACCGACTTTCAGCCCGGCCt < 1:1463393/69‑1 (MQ=255)
cGATCTGATCTTCAATCTTCACGCGACCATAACGAACAAAGAATGGTGTACCGACTTTCAGCCCGGCCt < 1:239113/69‑1 (MQ=255)
cGATCTGATCTTCAATCTTCACGCGACCATAACGAACAAAGAATGGTGTACCGACTTTCAGCCCGGCCt < 1:882118/69‑1 (MQ=255)
cGATCTGATCTTCAATCTTCACGCGACCATAACGAACAAAGAATGGTGTACCGACTTTCAGCCCGGCCt < 1:1362597/69‑1 (MQ=255)
cGATCTGATCTTCAATCTTCACGCGACCATAACGAACAAAGAATGGTGTACCGACTTTCAGCCCGGCCt < 1:2964260/69‑1 (MQ=255)
cGATCTGATCTTCAATCTTCACGCGACCATAACGAACAAAGAATGGTGTACCGACTTTCAGCCCGGCCt < 1:1273264/69‑1 (MQ=255)
cGATCTGATCTTCAATCTTCACGCGACCATAACGAACAAAGAATGGTGTACCGACTTTCAGCCCGGCCt < 1:820452/69‑1 (MQ=255)
gatctgatctTCAATCTTCACGCGACCATAACGAACAAAGAATGGTGTACCGACTTTCAGCCCGGCCt < 1:396515/68‑1 (MQ=255)
atcttcaatcttcaCGCGACCATAACGTACAAAGAATGGCGTACCGACTTTCAGCCCGGCTTGCTTCAg > 1:40782/1‑69 (MQ=255)
atcttcaatcttcaCGCGACCATAACGTACAAAGAATGGCGTACCGACTTTCAGCCCGGCTTGCTTCAg > 1:2408053/1‑69 (MQ=255)
atcttcaatcttcaCGCGACCATAACGTACAAAGAATGGCGTACCGACTTTCAGCCCGGCTTGCTTCAg > 1:510371/1‑69 (MQ=255)
cttcaatcttcaCGCGACCATAACGAACAAAGAATGGTGTACCGACTTTCAGCCCGGCCTGt < 1:3065587/62‑1 (MQ=255)
cttcaatcttcaCGCGACCATAACGAACAAAGAATGGTGTACCGACTTTCAGCCCGGCCTGt < 1:374880/62‑1 (MQ=255)
cttcaatcttcaCGCGACCATAACGAACAAAGAATGGTGTACCGACTTTCAGCCCGGCCTGt < 1:1705113/62‑1 (MQ=255)
cttcaatcttcaCGCGACCATAACGAACAAAGAATGGTGTACCGACTTTCAGCCCGGCCTGt < 1:2532493/62‑1 (MQ=255)
cttcaatcttcaCGCGACCATAACGAACAAAGAATGGTGTACCGACTTTCAGCCCGGCCTGt < 1:2610546/62‑1 (MQ=255)
cttcaatcttcaCGCGACCATAACGAACAAAGAATGGTGTACCGACTTTCAGCCCGGCCTGTTTCAgg > 1:919867/1‑68 (MQ=255)
cttcaatcttcaCGCGACCATAACGAACAAAGAATGGTGTACCGACTTTCAGCCCGGCCTGTTTCAGGc > 1:3042205/1‑69 (MQ=255)
ttcaatcttcaCGCGACCATAACGAACAAAGAATGGTGTACCGACTTTCAGCCCGGCCTGt < 1:2937952/61‑1 (MQ=255)
caatcttcaCGCGACCATAACGAACAAAGAATGGTGTACCGACTTTCAGCCCGGCCTGTTTc > 1:2121026/1‑62 (MQ=255)
caatcttcaCGCGACCATAACGAACAAAGAATGGTGTACCGACTTTCAGCCCGGCCTGTTTCAGGccc > 1:628993/1‑68 (MQ=255)
caatcttcaCGCGACCATAACGAACAAAGAATGGTGTACCGACTTTCAGCCCGGCCTGTTTCAGGccc > 1:2182979/1‑68 (MQ=255)
caatcttcaCGCGACCATAACGAACAAAGAATGGTGTACCGACTTTCAGCCCGGCCTGTTTCAGGccc > 1:1259770/1‑68 (MQ=255)
cttcaCGCGACCATAACGTACAAAGAATGGCGTACCGACTTTCAGCCCGGCTTGCTTCAGGCCCGCCAt < 1:3244234/69‑1 (MQ=255)
cttcaCGCGACCATAACGTACAAAGAATGGCGTACCGACTTTCAGCCCGGCTTGCTTCAGGCCCGCCAt < 1:681650/69‑1 (MQ=255)
cttcaCGCGACCATAACGTACAAAGAATGGCGTACCGACTTTCAGCCCGGCTTGCTTCAGGCCCGCCAt < 1:657807/69‑1 (MQ=255)
cttcaCGCGACCATAACGTACAAAGAATGGCGTACCGACTTTCAGCCCGGCTTGCTTCAGGCCCGCCAt < 1:1297474/69‑1 (MQ=255)
cttcaCGCGACCATAACGTACAAAGAATGGCGTACCGACTTTCAGCCCGGCTTGCTTCAGGCCCGCCAt < 1:1559236/69‑1 (MQ=255)
cttcaCGCGACCATAACGTACAAAGAATGGCGTACCGACTTTCAGCCCGGCTTGCTTCAGGCCCGCCAt < 1:1175814/69‑1 (MQ=255)
cttcaCGCGACCATAACGTACAAAGAATGGCGTACCGACTTTCAGCCCGGCTTGCTTCAGGCCCGCCAt < 1:366460/69‑1 (MQ=255)
cttcaCGCGACCATAACGTACAAAGAATGGCGTACCGACTTTCAGCCCGGCTTGCTTCAGGCCCGCCAt < 1:2094780/69‑1 (MQ=255)
cttcaCGCGACCATAACGTACAAAGAATGGCGTACCGACTTTCAGCCCGGCTTGCTTCAGGCCCGCCAt < 1:3122675/69‑1 (MQ=255)
cttcaCGCGACCATAACGTACAAAGAATGGCGTACCGACTTTCAGCCCGGCTTGCTTCAGGCCCGCCAt < 1:3029553/69‑1 (MQ=255)
cttcaCGCGACCATAACGTACAAAGAATGGCGTACCGACTTTCAGCCCGGCTTGCTTCAGGCCCGCCAt < 1:2274686/69‑1 (MQ=255)
cttcaCGCGACCATAACGTACAAAGAATGGCGTACCGACTTTCAGCCCGGCTTGCTTCAGGCCCGCCAt < 1:2785520/69‑1 (MQ=255)
cttcaCGCGACCATAACGTACAAAGAATGGCGTACCGACTTTCAGCCCGGCTTGCTTCAGGCCCGCCAt < 1:1001378/69‑1 (MQ=255)
cttcaCGCGACCATAACATACAAAGAATGGCGTACCGACTTTCAGCCCGGCTTGCTTCAGGCCCGCCAt < 1:2685841/69‑1 (MQ=255)
ttcaCGCGACCATAACGAACAAAGAATGGTGTACCGACTTTCAGCCCGGCCTGTTTCAGGCCCGCCATc > 1:1926970/1‑69 (MQ=255)
ttcaCGCGACCATAACGAACAAAGAATGGTGTACCGACTTTCAGCCCGGCCTGTTTCAGGCCCGCCATc > 1:1030650/1‑69 (MQ=255)
ttcaCGCGACCATAACGAACAAAGAATGGTGTACCGACTTTCAGCCCGGCCTGTTTCAGGCCCGCCATc > 1:680004/1‑69 (MQ=255)
ttcaCGCGACCATAACGAACAAAGAATGGTGTACCGACTTTCAGCCCGGCCTGTTTCAGGCCCGCCATc > 1:2290453/1‑69 (MQ=255)
tcaCGCGACCATAACGTACAAAGAATGGCGTACCGACTTTCAGCCCGGCTTGCTTCAGGCCCGCCAt < 1:3042524/67‑1 (MQ=255)
cATAACGAACAAAGAATGGTGTACC‑ACTTTCAGCCCGGCCTGt < 1:1458480/43‑1 (MQ=38)
tAACGAACAAAGAATGGTGTACCGACTTTCAGCCCGGCCt < 1:1638221/40‑1 (MQ=255)
aCGAACAAAGAATGGTGTACCGACTTTCAGCCCGGCCTGTTTCAGGCCCGCCATCAGCGGt > 1:834728/1‑61 (MQ=255)
|
ATCACTACTTTCGCGCCGAGGATCTCACCGATCTGATCTTCAATCTTCACGCGACCATAACGAACAAAGAATGGTGTACCGACTTTCAGCCCGGCCTGTTTCAGGCCCGCCATCAGCGGT > minE/1609688‑1609807
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
