Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,609,783 |
C→T |
34.9% |
Q191Q (CAG→CAA) |
eutC ← |
ethanolamine ammonia‑lyase, small subunit (light chain) |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,609,783 | 0 | C | T | 34.9%
| 62.5
/ 42.8
| 80 | Q191Q (CAG→CAA) | eutC | ethanolamine ammonia‑lyase, small subunit (light chain) |
| Reads supporting (aligned to +/- strand): ref base C (28/24); new base T (3/25); total (31/49) |
| Fisher's exact test for biased strand distribution p-value = 1.22e-04 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.22e-01 |
CGATCTGATCTTCAATCTTCACGCGACCATAACGAACAAAGAATGGTGTACCGACTTTCAGCCCGGCCTGTTTCAGGCCCGCCATCAGCGGTGGCAGGATCTCTTCGTAGTTCACGGTGATCG > minE/1609716‑1609838
|
cGATCTGATCTTCAATCTTCACGCGACCATAACGAACAAAGAATGGTGTACCGACTTTCAGCCCGGCCt < 1:239113/69‑1 (MQ=255)
cGATCTGATCTTCAATCTTCACGCGACCATAACGAACAAAGAATGGTGTACCGACTTTCAGCCCGGCCt < 1:820452/69‑1 (MQ=255)
cGATCTGATCTTCAATCTTCACGCGACCATAACGAACAAAGAATGGTGTACCGACTTTCAGCCCGGCCt < 1:1544919/69‑1 (MQ=255)
cGATCTGATCTTCAATCTTCACGCGACCATAACGAACAAAGAATGGTGTACCGACTTTCAGCCCGGCCt < 1:1273264/69‑1 (MQ=255)
cGATCTGATCTTCAATCTTCACGCGACCATAACGAACAAAGAATGGTGTACCGACTTTCAGCCCGGCCt < 1:1718466/69‑1 (MQ=255)
cGATCTGATCTTCAATCTTCACGCGACCATAACGAACAAAGAATGGTGTACCGACTTTCAGCCCGGCCt < 1:2964260/69‑1 (MQ=255)
cGATCTGATCTTCAATCTTCACGCGACCATAACGAACAAAGAATGGTGTACCGACTTTCAGCCCGGCCt < 1:882118/69‑1 (MQ=255)
cGATCTGATCTTCAATCTTCACGCGACCATAACGAACAAAGAATGGTGTACCGACTTTCAGCCCGGCCt < 1:1362597/69‑1 (MQ=255)
cGATCTGATCTTCAATCTTCACGCGACCATAACGAACAAAGAATGGTGTACCGACTTTCAGCCCGGCCt < 1:1492850/69‑1 (MQ=255)
cGATCTGATCTTCAATCTTCACGCGACCATAACGAACAAAGAATGGTGTACCGACTTTCAGCCCGGCCt < 1:1463393/69‑1 (MQ=255)
gatctgatctTCAATCTTCACGCGACCATAACGAACAAAGAATGGTGTACCGACTTTCAGCCCGGCCt < 1:396515/68‑1 (MQ=255)
atcttcaatcttcaCGCGACCATAACGTACAAAGAATGGCGTACCGACTTTCAGCCCGGCTTGCTTCAg > 1:2408053/1‑69 (MQ=255)
atcttcaatcttcaCGCGACCATAACGTACAAAGAATGGCGTACCGACTTTCAGCCCGGCTTGCTTCAg > 1:510371/1‑69 (MQ=255)
atcttcaatcttcaCGCGACCATAACGTACAAAGAATGGCGTACCGACTTTCAGCCCGGCTTGCTTCAg > 1:40782/1‑69 (MQ=255)
cttcaatcttcaCGCGACCATAACGAACAAAGAATGGTGTACCGACTTTCAGCCCGGCCTGt < 1:1705113/62‑1 (MQ=255)
cttcaatcttcaCGCGACCATAACGAACAAAGAATGGTGTACCGACTTTCAGCCCGGCCTGt < 1:2610546/62‑1 (MQ=255)
cttcaatcttcaCGCGACCATAACGAACAAAGAATGGTGTACCGACTTTCAGCCCGGCCTGt < 1:3065587/62‑1 (MQ=255)
cttcaatcttcaCGCGACCATAACGAACAAAGAATGGTGTACCGACTTTCAGCCCGGCCTGt < 1:374880/62‑1 (MQ=255)
cttcaatcttcaCGCGACCATAACGAACAAAGAATGGTGTACCGACTTTCAGCCCGGCCTGt < 1:2532493/62‑1 (MQ=255)
cttcaatcttcaCGCGACCATAACGAACAAAGAATGGTGTACCGACTTTCAGCCCGGCCTGTTTCAgg > 1:919867/1‑68 (MQ=255)
cttcaatcttcaCGCGACCATAACGAACAAAGAATGGTGTACCGACTTTCAGCCCGGCCTGTTTCAGGc > 1:3042205/1‑69 (MQ=255)
ttcaatcttcaCGCGACCATAACGAACAAAGAATGGTGTACCGACTTTCAGCCCGGCCTGt < 1:2937952/61‑1 (MQ=255)
caatcttcaCGCGACCATAACGAACAAAGAATGGTGTACCGACTTTCAGCCCGGCCTGTTTc > 1:2121026/1‑62 (MQ=255)
caatcttcaCGCGACCATAACGAACAAAGAATGGTGTACCGACTTTCAGCCCGGCCTGTTTCAGGccc > 1:1259770/1‑68 (MQ=255)
caatcttcaCGCGACCATAACGAACAAAGAATGGTGTACCGACTTTCAGCCCGGCCTGTTTCAGGccc > 1:628993/1‑68 (MQ=255)
caatcttcaCGCGACCATAACGAACAAAGAATGGTGTACCGACTTTCAGCCCGGCCTGTTTCAGGccc > 1:2182979/1‑68 (MQ=255)
cttcaCGCGACCATAACGTACAAAGAATGGCGTACCGACTTTCAGCCCGGCTTGCTTCAGGCCCGCCAt < 1:2785520/69‑1 (MQ=255)
cttcaCGCGACCATAACGTACAAAGAATGGCGTACCGACTTTCAGCCCGGCTTGCTTCAGGCCCGCCAt < 1:3029553/69‑1 (MQ=255)
cttcaCGCGACCATAACGTACAAAGAATGGCGTACCGACTTTCAGCCCGGCTTGCTTCAGGCCCGCCAt < 1:366460/69‑1 (MQ=255)
cttcaCGCGACCATAACGTACAAAGAATGGCGTACCGACTTTCAGCCCGGCTTGCTTCAGGCCCGCCAt < 1:3122675/69‑1 (MQ=255)
cttcaCGCGACCATAACGTACAAAGAATGGCGTACCGACTTTCAGCCCGGCTTGCTTCAGGCCCGCCAt < 1:3244234/69‑1 (MQ=255)
cttcaCGCGACCATAACGTACAAAGAATGGCGTACCGACTTTCAGCCCGGCTTGCTTCAGGCCCGCCAt < 1:2274686/69‑1 (MQ=255)
cttcaCGCGACCATAACGTACAAAGAATGGCGTACCGACTTTCAGCCCGGCTTGCTTCAGGCCCGCCAt < 1:1001378/69‑1 (MQ=255)
cttcaCGCGACCATAACGTACAAAGAATGGCGTACCGACTTTCAGCCCGGCTTGCTTCAGGCCCGCCAt < 1:2094780/69‑1 (MQ=255)
cttcaCGCGACCATAACGTACAAAGAATGGCGTACCGACTTTCAGCCCGGCTTGCTTCAGGCCCGCCAt < 1:1297474/69‑1 (MQ=255)
cttcaCGCGACCATAACGTACAAAGAATGGCGTACCGACTTTCAGCCCGGCTTGCTTCAGGCCCGCCAt < 1:1559236/69‑1 (MQ=255)
cttcaCGCGACCATAACGTACAAAGAATGGCGTACCGACTTTCAGCCCGGCTTGCTTCAGGCCCGCCAt < 1:1175814/69‑1 (MQ=255)
cttcaCGCGACCATAACGTACAAAGAATGGCGTACCGACTTTCAGCCCGGCTTGCTTCAGGCCCGCCAt < 1:681650/69‑1 (MQ=255)
cttcaCGCGACCATAACGTACAAAGAATGGCGTACCGACTTTCAGCCCGGCTTGCTTCAGGCCCGCCAt < 1:657807/69‑1 (MQ=255)
cttcaCGCGACCATAACATACAAAGAATGGCGTACCGACTTTCAGCCCGGCTTGCTTCAGGCCCGCCAt < 1:2685841/69‑1 (MQ=255)
ttcaCGCGACCATAACGAACAAAGAATGGTGTACCGACTTTCAGCCCGGCCTGTTTCAGGCCCGCCATc > 1:680004/1‑69 (MQ=255)
ttcaCGCGACCATAACGAACAAAGAATGGTGTACCGACTTTCAGCCCGGCCTGTTTCAGGCCCGCCATc > 1:1030650/1‑69 (MQ=255)
ttcaCGCGACCATAACGAACAAAGAATGGTGTACCGACTTTCAGCCCGGCCTGTTTCAGGCCCGCCATc > 1:1926970/1‑69 (MQ=255)
ttcaCGCGACCATAACGAACAAAGAATGGTGTACCGACTTTCAGCCCGGCCTGTTTCAGGCCCGCCATc > 1:2290453/1‑69 (MQ=255)
tcaCGCGACCATAACGTACAAAGAATGGCGTACCGACTTTCAGCCCGGCTTGCTTCAGGCCCGCCAt < 1:3042524/67‑1 (MQ=255)
cATAACGAACAAAGAATGGTGTACC‑ACTTTCAGCCCGGCCTGt < 1:1458480/43‑1 (MQ=38)
tAACGAACAAAGAATGGTGTACCGACTTTCAGCCCGGCCt < 1:1638221/40‑1 (MQ=255)
aCGAACAAAGAATGGTGTACCGACTTTCAGCCCGGCCTGTTTCAGGCCCGCCATCAGCGGt > 1:834728/1‑61 (MQ=255)
cgtaCAAAGAATGGCGTACCGACTTTCAGCCCGGCTTGCTTCAGGCCCGCCATCAGCggcggc < 1:3287852/60‑1 (MQ=255)
cgtaCAAAGAATGGCGTACCGACTTTCAGCCCGGCTTGCTTCAGGCCCGCCATCAGCggcggc < 1:3238224/60‑1 (MQ=255)
cgtaCAAAGAATGGCGTACCGACTTTCAGCCCGGCTTGCTTCAGGCCCGCCATCAGCggcggc < 1:1238814/60‑1 (MQ=255)
cgtaCAAAGAATGGCGTACCGACTTTCAGCCCGGCTTGCTTCAGGCCCGCCATCAGCggcggc < 1:3408091/60‑1 (MQ=255)
cgtaCAAAGAATGGCGTACCGACTTTCAGCCCGGCTTGCTTCAGGCCCGCCATCAGCggcggc < 1:1253697/60‑1 (MQ=255)
cgtaCAAAGAATGGCGTACCGACTTTCAGCCCGGCTTGCTTCAGGCCCGCCATCAGCggcggc < 1:927461/60‑1 (MQ=255)
cgtaCAAAGAATGGCGTACCGACTTTCAGCCCGGCTTGCTTCAGGCCCGCCATCAGCggcggc < 1:2856868/60‑1 (MQ=255)
cgtaCAAAGAATGGCGTACCGACTTTCAGCCCGGCTTGCTTCAGGCCCGCCATCAGCggcggc < 1:2747526/60‑1 (MQ=255)
cgtaCAAAGAATGGCGTACCGACTTTCAGCCCGGCTTGCTTCAGGCCCGCCATCAGCggcggc < 1:177821/60‑1 (MQ=255)
taCAAAGAATGGCGTACCGACTTTCAGCCCGGCTTGCTTCAGGCCCGCCATCAGCggcggc < 1:249569/60‑1 (MQ=255)
tACCGACTTTCAGCCCGGCCTGTTTCAGGCCCGCCATCAGCGGTTGCAGGATCTCTTCGt > 1:120797/1‑60 (MQ=255)
tACCGACTTTCAGCCCGGCCTGTTTCAGGCCCGCCATCAGCGGTGGCAGGATCTCTTCGt > 1:430095/1‑60 (MQ=255)
tACCGACTTTCAGCCCGGCCTGTTTCAGGCCCGCCATCAGCGGTGGCAGGATCTCTTCGt > 1:1022606/1‑60 (MQ=255)
tACCGACTTTCAGCCCGGCCTGTTTCAGGCCCGCCATCAGCGGTGGCAGGATCTCTTCGt > 1:562216/1‑60 (MQ=255)
tACCGACTTTCAGCCCGGCCTGTTTCAGGCCCGCCATCAGCGGTGGCAGGATCTCTTCGt > 1:1361907/1‑60 (MQ=255)
tACCGACTTTCAGCCCGGCCTGTTTCAGGCCCGCCATCAGCGGTGGCAGGATCTCTTCGt > 1:2930773/1‑60 (MQ=255)
tACCGACTTTCAGCCCGGCCTGTTTCAGGCCCGCCATCAGCGGTGGCAGGATCTCTTCGt > 1:2866608/1‑60 (MQ=255)
tACCGACTTTCAGCCCGGCCTGTTTCAGGCCCGCCATCAGCGGTGGCAGGATCTCTTCGt > 1:2792325/1‑60 (MQ=255)
tACCGACTTTCAGCCCGGCCTGTTTCAGGCCCGCCATCAGCGGTGGCAGGATCTCTTCGt > 1:1979504/1‑60 (MQ=255)
tACCGACTTTCAGCCCGGCCTGTTTCAGGCCCGCCATCAGCGGTGGCAGGATCTCTTCGt > 1:1917726/1‑60 (MQ=255)
tACCGACTTTCAGCCCGGCCTGTTTCAGGCCCGCCATCAGCGGTGGCAGGATCTCTTCGt > 1:1716998/1‑60 (MQ=255)
tACCGACTTTCAGCCCGGCCTGTTTCAGGCCCGCCATCAGCGGTGGCAGGATCTCTTCGt > 1:155805/1‑60 (MQ=255)
tACCGACTTTCAGCCCGGCCTGTTTCAGGCCCGCCATCAGCGGTGGCAGGATCTCTTCGt > 1:1528359/1‑60 (MQ=255)
ccGACTTTCAGCCCGGCCTGTTTCAGGCCCGCCATCAGCGGTGGCAGGATCTCTTCGTAGTTCACGGt < 1:1352729/68‑1 (MQ=255)
ccGACTTTCAGCCCGGCCTGTTTCAGGCCCGCCATCAGCGGTGGCAGGATCTCTTCGTAGTTCACGGt < 1:2767967/68‑1 (MQ=255)
ccGACTTTCAGCCCGGCCTGTTTCAGGCCCGCCATCAGCGGTGGCAGGATCTCTTCGTAGTTCACGGt < 1:1685902/68‑1 (MQ=255)
ccGACTTTCAGCCCGGCCTGTTTCAGGCCCGCCATCAGCGGTGGCAGGATCTCTTCGTAGTTCACGGTg > 1:1894031/1‑69 (MQ=255)
gACTTTCAGCCCGGCCTGTTTCAGGCCCGCCATCAGCGGTGGCAGGATCTCTTCGt > 1:1778973/1‑56 (MQ=255)
gACTTTCAGCCCGGCCTGTTTCAGGCCCGCCATCAGCGGTGGCAGGATCTCTTCGTAGTTCACGGTGAt > 1:941801/1‑69 (MQ=255)
gACTTTCAGCCCGGCCTGTTTCAGGCCCGCCATCAGCGGTGGCAGGATCTCTTCGTAGTTCACGGTGAt > 1:3101441/1‑69 (MQ=255)
cTTTCAGCCCGGCCTGTTTCAGGCCCGCCATCAGCGGTGGCAGGATCTCTTCGTAGTTCACGGTGATCg < 1:3103229/69‑1 (MQ=255)
cTTTCAGCCCGGCCTGTTTCAGGCCCGCCATCAGCGGTGGCAGGATCTCTTCGTAGTTCACGGTGATCg < 1:3292912/69‑1 (MQ=255)
|
CGATCTGATCTTCAATCTTCACGCGACCATAACGAACAAAGAATGGTGTACCGACTTTCAGCCCGGCCTGTTTCAGGCCCGCCATCAGCGGTGGCAGGATCTCTTCGTAGTTCACGGTGATCG > minE/1609716‑1609838
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
