Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,610,889 |
G→A |
46.5% |
C283C (TGC→TGT) |
eutB ← |
ethanolamine ammonia‑lyase, large subunit, heavy chain |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,610,889 | 0 | G | A | 46.5%
| 2.9
/ 33.1
| 43 | C283C (TGC→TGT) | eutB | ethanolamine ammonia‑lyase, large subunit, heavy chain |
| Reads supporting (aligned to +/- strand): ref base G (14/9); new base A (7/13); total (21/22) |
| Fisher's exact test for biased strand distribution p-value = 1.29e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.20e-01 |
AGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCAGTTTTCCCCGGCGATACGGTTGAACTCCGCGCCCACTGCGCGCGCT > minE/1610844‑1610937
|
aGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCAGTTTTCCCCGGCGATACGGt > 1:2779911/1‑68 (MQ=255)
aGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCAGTTTTCCCCGGCGATACGGt > 1:1353132/1‑68 (MQ=255)
aGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCAGTTTTCCCCGGCGATACGGt > 1:1355978/1‑68 (MQ=255)
aGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCAGTTTTCCCCGGCGATACGGt > 1:3273839/1‑68 (MQ=255)
aGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCAGTTTTCCCCGGCGATACGGt > 1:2245818/1‑68 (MQ=255)
aGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCAGTTTTCCCCGGCGATACGGt > 1:2203472/1‑68 (MQ=255)
tAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCAGTTTTCCCCGGCGATACGGTTGAACTCCGCGcc < 1:2002504/69‑1 (MQ=255)
tAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCAGTTTTCCCCGGCGATACGGTTGAACTCCGCGcc < 1:1998471/69‑1 (MQ=255)
cgcCGAGCCTTGTCCGGTTTCGAAGTAGAGACAGTTTTCCCCGGCGATACGGTTGAACTCCGCGCCCAc > 1:1269724/1‑69 (MQ=255)
cgcCGAGCCTTGTCCGGTTTCGAAGTAGAGACAGTTTTCCCCGGCGATACGGTTGAACTCCGCGCCCAc > 1:3192928/1‑69 (MQ=255)
cgcCGAGCCTTGTCCGGTTTCGAAGTAGAGACAGTTTTCCCCGGCGATACGGTTGAACTCCGCGCCCAc > 1:2768371/1‑69 (MQ=255)
cgcCGAGCCTTGTCCGGTTTCGAAGTAGAGACAGTTTTCCCCGGCGATACGGTTGAACTCCGCGCCCAc > 1:2537355/1‑69 (MQ=255)
cgcCGAGCCTTGTCCGGTTTCGAAGTAGAGACAGTTTTCCCCGGCGATACGGTTGAACTCCGCGCCCAc > 1:82689/1‑69 (MQ=255)
cgcCGAGCCTTGTCCGGTTTCGAAGTAGAGACAGTTTTCCCCGGCGATACGGTTGAACTCCGCGCCCAc > 1:1569268/1‑69 (MQ=255)
cgcCGAGCCTTGTCCGGTTTCGAAGTAGAGACAGGTTTCCCCGGCGATACGGTTGAACTCCGCGCCCAc > 1:3220171/1‑69 (MQ=255)
gCCTTGTCCGGTTTCGAAGTAGAGGCAGTTTTCCCCGGCGATAc > 1:2951729/1‑44 (MQ=255)
gCCTTGTCCGGTTTCGAAGTAGAGGCAGTTTTCCCCGGCGATAc > 1:1344329/1‑44 (MQ=255)
gCCTTGTCCGGTTTCGAAGTAGAGGCAGTTTTCCCCGGCGATAc < 1:1039022/44‑1 (MQ=255)
gCCTTGTCCGGTTTCGAAGTAGAGGCAGTTTTCCCCGGCGATAc > 1:2732979/1‑44 (MQ=255)
gCCTTGTCCGGTTTCGAAGTAGAGGCAGTTTTCCCCGGCGATAc < 1:1481749/44‑1 (MQ=255)
gCCTTGTCCGGTTTCGAAGTAGAGGCAGTTTTCCCCGGCGATAc < 1:2696627/44‑1 (MQ=255)
gCCTTGTCCGGTTTCGAAGTAGAGGCAGTTTTCCCCGGCGATAc > 1:255887/1‑44 (MQ=255)
gCCTTGTCCGGTTTCGAAGTAGAGGCAGTTTTCCCCGGCGATAc < 1:1877697/44‑1 (MQ=255)
gCCTTGTCCGGTTTCGAAGTAGAGGCAGTTTTCCCCGGCGATAc < 1:3301059/44‑1 (MQ=255)
gCCTTGTCCGGTTTCGAAGTAGAGGCAGTTTTCCCCGGCGATAc > 1:2472639/1‑44 (MQ=255)
gCCTTGTCCGGTTTCGAAGTAGAGGCAGTTTTCCCCGGCGATAc > 1:2356273/1‑44 (MQ=255)
gCCTTGTCCGGTTTCGAAGTAGAGGCAGTTTTCCCCGGCGATAc < 1:827701/44‑1 (MQ=255)
gCCTTGTCCGGTTTCGAAGTAGAGGCAGTTTTCCCCGGCGATAc > 1:2242668/1‑44 (MQ=255)
gCCTTGTCCGGTTTCGAAGTAGAGGCAGTTTTCCCCGGCGATAc > 1:3404716/1‑44 (MQ=255)
ccTTGTCCGGTTTCGAAGTAGAGGCAGTTTTCCCCGGCGATAc < 1:2834453/43‑1 (MQ=255)
tGTCCGGTTTCGAAGTAGAGACAGTTTTCCCCGGCGATACGGTTGAACTCCGCGCCCACTGCGCGTGCt < 1:828916/69‑1 (MQ=255)
tGTCCGGTTTCGAAGTAGAGACAGTTTTCCCCGGCGATACGGTTGAACTCCGCGCCCACTGCGCGTGCt < 1:996661/69‑1 (MQ=255)
tGTCCGGTTTCGAAGTAGAGACAGTTTTCCCCGGCGATACGGTTGAACTCCGCGCCCACTGCGCGTGCt < 1:568803/69‑1 (MQ=255)
tGTCCGGTTTCGAAGTAGAGACAGTTTTCCCCGGCGATACGGTTGAACTCCGCGCCCACTGCGCGTGCt < 1:2860712/69‑1 (MQ=255)
tGTCCGGTTTCGAAGTAGAGACAGTTTTCCCCGGCGATACGGTTGAACTCCGCGCCCACTGCGCGTGCt < 1:2803665/69‑1 (MQ=255)
tGTCCGGTTTCGAAGTAGAGACAGTTTTCCCCGGCGATACGGTTGAACTCCGCGCCCACTGCGCGTGCt < 1:2711342/69‑1 (MQ=255)
tGTCCGGTTTCGAAGTAGAGACAGTTTTCCCCGGCGATACGGTTGAACTCCGCGCCCACTGCGCGTGCt < 1:2477371/69‑1 (MQ=255)
tGTCCGGTTTCGAAGTAGAGACAGTTTTCCCCGGCGATACGGTTGAACTCCGCGCCCACTGCGCGTGCt < 1:2059545/69‑1 (MQ=255)
tGTCCGGTTTCGAAGTAGAGACAGTTTTCCCCGGCGATACGGTTGAACTCCGCGCCCACTGCGCGTGCt < 1:1695411/69‑1 (MQ=255)
tGTCCGGTTTCGAAGTAGAGACAGTTTTCCCCGGCGATACGGTTGAACTCCGCGCCCACTGCGCGTGCt < 1:159905/69‑1 (MQ=255)
tGTCCGGTTTCGAAGTAGAGACAGTTTTCCCCGGCGATACGGTTGAACTCCGCGCCCACTGCGCGTGCt < 1:1539465/69‑1 (MQ=255)
tGTCCGGTTTCGAAGTAGAGACAGTTTTCCCCGGCGATACGGTTGAACTCCGCGCCCACTGCGCGTGCt < 1:1504128/69‑1 (MQ=255)
tGTCCGGTTTCGAAGTAGAGACAGTTTTCCCCGGCGATACGGTTGAACTCCGCGCCCACTGCGCGTGCt < 1:1469656/69‑1 (MQ=255)
|
AGCGCCAGCGGATAGCGCAGAGCCTTGTCCGGTTTCGAAGTAGAGGCAGTTTTCCCCGGCGATACGGTTGAACTCCGCGCCCACTGCGCGCGCT > minE/1610844‑1610937
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
