Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,639,640 |
A→G |
60.1% |
A133A (GCT→GCC) |
ypfH ← |
predicted hydrolase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,639,640 | 0 | A | G | 60.1%
| 31.0
/ 46.8
| 70 | A133A (GCT→GCC) | ypfH | predicted hydrolase |
| Reads supporting (aligned to +/- strand): ref base A (7/21); new base G (22/20); total (29/41) |
| Fisher's exact test for biased strand distribution p-value = 2.77e-02 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.42e-01 |
GAATCAGGTGAATTGTCGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCC > minE/1639576‑1639702
|
gAATCAAGTGACTTGTTGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAGGCaa < 1:3104448/69‑1 (MQ=255)
gAATCAAGTGAATTGTTGTGTCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAGGCaa < 1:1616058/69‑1 (MQ=255)
gAATCAAGTGAATTGTTGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAGGCaa < 1:98706/69‑1 (MQ=255)
gAATCAAGTGAATTGTTGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAGGCaa < 1:3208103/69‑1 (MQ=255)
gAATCAAGTGAATTGTTGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAGGCaa < 1:707741/69‑1 (MQ=255)
gAATCAAGTGAATTGTTGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAGGCaa < 1:2554656/69‑1 (MQ=255)
gAATCAAGTGAATTGTTGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAGGCaa < 1:2278969/69‑1 (MQ=255)
gAATCAAGTGAATTGTTGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAGGCaa < 1:218441/69‑1 (MQ=255)
gAATCAAGTGAATTGTTGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAGGCaa < 1:2101012/69‑1 (MQ=255)
gAATCAAGTGAATTGTTGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAGGCaa < 1:2095870/69‑1 (MQ=255)
gAATCAAGTGAATTGTTGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAGGCaa < 1:717826/69‑1 (MQ=255)
gAATCAAGTGAATTGTTGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAGGCaa < 1:87440/69‑1 (MQ=255)
gAATCAAGTGAATTGTTGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAGGCaa < 1:163753/69‑1 (MQ=255)
gAATCAAGTGAATTGTTGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAGGCaa < 1:1559821/69‑1 (MQ=255)
gAATCAAGTGAATTGTTGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAGGCaa < 1:1052038/69‑1 (MQ=255)
gAATCAAGTGAATTGTTGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAGGCaa < 1:1089191/69‑1 (MQ=255)
gAATCAAGTGAATTGTTGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAGGCaa < 1:985144/69‑1 (MQ=255)
aaTCAAGTGAATTGTTGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAGGCaa < 1:770605/68‑1 (MQ=255)
aaTCAAGTGAATTGTTGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAGGCaa < 1:1930508/68‑1 (MQ=255)
gAATTGTCGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGc < 1:619585/58‑1 (MQ=255)
gAATTGTCGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGc < 1:754930/58‑1 (MQ=255)
gAATTGTCGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGc < 1:1887950/58‑1 (MQ=255)
gAATTGTCGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGc < 1:629067/58‑1 (MQ=255)
gAATTGTCGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGc < 1:1631884/58‑1 (MQ=255)
gAATTGTCGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGc < 1:2941823/58‑1 (MQ=255)
gAATTGTCGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGc < 1:1218332/58‑1 (MQ=255)
aaTTGTCGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGc < 1:1001633/57‑1 (MQ=255)
tGTCGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGc < 1:2847773/69‑1 (MQ=255)
gCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGccc > 1:284923/1‑69 (MQ=255)
gACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAGGCaa < 1:1498108/43‑1 (MQ=255)
tCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATg < 1:3271286/69‑1 (MQ=255)
tCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATg > 1:2616148/1‑69 (MQ=255)
tCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATg < 1:627674/69‑1 (MQ=255)
tCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATg < 1:1943890/69‑1 (MQ=255)
tAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATa < 1:3270919/69‑1 (MQ=255)
tAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATa < 1:2896188/69‑1 (MQ=255)
tAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATa < 1:2895963/69‑1 (MQ=255)
tAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATa < 1:346286/69‑1 (MQ=255)
tAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATa < 1:2760691/69‑1 (MQ=255)
tAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATa < 1:229065/69‑1 (MQ=255)
tAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATa < 1:11085/69‑1 (MQ=255)
tAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATa < 1:742006/69‑1 (MQ=255)
tAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATa < 1:819227/69‑1 (MQ=255)
gCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATaa > 1:3203415/1‑68 (MQ=255)
gCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAAt > 1:263708/1‑69 (MQ=255)
gCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAAt > 1:3039347/1‑69 (MQ=255)
gCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAAt > 1:3315591/1‑69 (MQ=255)
gCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAAt > 1:526054/1‑69 (MQ=255)
ttAAAGGCAATGACGCGTGAGGCAAGGCCCGGTTCGGCTTTAATGCTCTCCATCGCCATAATCGCGcc > 1:1047946/1‑68 (MQ=255)
ttAAAGGCAATGACGCGTGAGGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCGCCATAATCgcgc > 1:46990/1‑67 (MQ=255)
ttAAAGGCAATGACGCGTGAGGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCGCCATAATCGCGcc > 1:649611/1‑68 (MQ=255)
ttAAAGGCAATGACGCGTGAGGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCGCCATAATCGCGcc > 1:2827321/1‑68 (MQ=255)
ttAAAGGCAATGACGCGTGAGGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCGCCATAATCGCGcc > 1:708863/1‑68 (MQ=255)
ttAAAGGCAATGACGCGTGAGGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCGCCATAATCGCGcc > 1:950482/1‑68 (MQ=255)
ttAAAGGCAATGACGCGTGAGGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCGCCATAATCGCGcc > 1:90058/1‑68 (MQ=255)
ttAAAGGCAATGACGCGTGAGGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCGCCATAATCGCGcc > 1:2040516/1‑68 (MQ=255)
ttAAAGGCAATGACGCGTGAGGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCGCCATAATCGCGcc > 1:1033027/1‑68 (MQ=255)
ttAAAGGCAATGACGCGTGAGGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCGCCATAATCGCGcc > 1:1422416/1‑68 (MQ=255)
ttAAAGGCAATGACGCGTGAGGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCGCCATAATCGCGcc > 1:1662318/1‑68 (MQ=255)
ttAAAGGCAATGACGCGTGAGGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCGCCATAATCGCGcc > 1:1707283/1‑68 (MQ=255)
ttAAAGGCAATGACGCGTGAGGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCGCCATAATCGCGcc > 1:1737069/1‑68 (MQ=255)
ttAAAGGCAATGACGCGTGAGGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCGCCATAATCGCGcc > 1:1745458/1‑68 (MQ=255)
ttAAAGGCAATGACGCGTGAGGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCGCCATAATCGCGcc > 1:491806/1‑68 (MQ=255)
ttAAAGGCAATGACGCGTGAGGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCGCCATAATCGCGcc > 1:2074712/1‑68 (MQ=255)
ttAAAGGCAATGACGCGTGAGGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCGCCATAATCGCGcc > 1:2119961/1‑68 (MQ=255)
ttAAAGGCAATGACGCGTGAGGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCGCCATAATCGCGcc > 1:2571231/1‑68 (MQ=255)
ttAAAGGCAATGACGCGTGAGGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCGCCATAATCGCGcc > 1:2825548/1‑68 (MQ=255)
ttAAAGGCAATGACGCGTGAGGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCGCCATAATCGCGcc > 1:3305928/1‑68 (MQ=255)
ttAAAGGCAATGACGCGTGAGGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCGCCATAATCGCGcc > 1:3324512/1‑68 (MQ=255)
ttAAAGGCAATGACGCGTGAGGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCGCCATAATCGCGcc > 1:3400380/1‑68 (MQ=255)
aaaaGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGcc > 1:2783087/1‑66 (MQ=255)
aaaaGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGcc > 1:834926/1‑66 (MQ=255)
aaaaGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGcc > 1:1517284/1‑66 (MQ=255)
aaaaGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGcc > 1:1301349/1‑66 (MQ=255)
|
GAATCAGGTGAATTGTCGTGGCTGTAGACGCAGTTTCCGGCAGGCTCGCATAGCGTCCGTTAAAAGCAATGACGCGTGAAGCAAGGCCCGGTTCGGCTTTAATGCTCTCCAGCACCATAATCGCGCC > minE/1639576‑1639702
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
