Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,649,712 |
A→G |
43.7% |
A311A (GCA→GCG) |
yfgC → |
predicted peptidase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,649,712 | 0 | A | G | 43.7%
| 25.1
/ 91.1
| 112 | A311A (GCA→GCG) | yfgC | predicted peptidase |
| Reads supporting (aligned to +/- strand): ref base A (40/23); new base G (13/36); total (53/59) |
| Fisher's exact test for biased strand distribution p-value = 1.27e-04 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01 |
Only 100 of 112 total aligned reads displayed.
AGCTCACCAGTGATTTGCTGGATGAATGGGCGAAAGGAAACGTTCGTCAGCAACGAGCAGCACAATATGGTCGTGCTTTACAGGCGATGGAAGCCAATAAATACGACGAGGCGCGTAA > minE/1649651‑1649768
|
aGCTCACCAGTGATTTGCTGGATGAATGGGCGAAAGGAAACGTTCGTCAGCAACGAGCAGCACAATATg < 1:1791723/69‑1 (MQ=255)
aGCTCACCAGTGATTTGCTGGATGAATGGGCGAAAGGAAACGTTCGTCAGCAACGAGCAGCACAATATg < 1:2286038/69‑1 (MQ=255)
aGCTCACCAGTGATTTGCTGGATGAATGGGCGAAAGGAAACGTTCGTCAGCAACGAGCAGCACAATATg < 1:1306629/69‑1 (MQ=255)
aGCTCACCAGTGATTTGCTGGATGAATGGGCGAAAGGAAACGTTCGTCAGCAACGAGCAGCACAATATg < 1:877009/69‑1 (MQ=255)
aGCTCACCAGTGATTTGCTGGATGAATGGGCGAAAGGAAACGTTCGTCAGCAACGAGCAGCACAATATg < 1:2365256/69‑1 (MQ=255)
gCTCACCAGTGATTTGCTGGATGAATGGGCGAAAGGAAACGTTCGTCAGCAACGAGCAGCACAATATg < 1:3406800/68‑1 (MQ=255)
aGTGATTTGCTGGATGAATGGGCGAAAGGAAACGTTCGTCAGCAACGAGCAGCACAATATGGTCGTg > 1:708936/1‑67 (MQ=255)
aGTGATTTGCTGGATGAATGGGCGAAAGGAAACGTTCGTCAGCAACGAGCAGCACAATATGGTCGTGc > 1:1690189/1‑68 (MQ=255)
aGTGATTTGCTGGATGAATGGGCGAAAGGAAACGTTCGTCAGCAACGAGCAGCACAATATGGTCGTGc > 1:968699/1‑68 (MQ=255)
aGTGATTTGCTGGATGAATGGGCGAAAGGAAACGTTCGTCAGCAACGAGCAGCACAATATGGTCGTGc > 1:2308681/1‑68 (MQ=255)
aGTGATTTGCTGGATGAATGGGCGAAAGGAAACGTTCGTCAGCAACGAGCAGCACAATATGGTCGTGc > 1:2633107/1‑68 (MQ=255)
aGTGATTTGCTGGATGAATGGGCGAAAGGAAACGTTCGTCAGCAACGAGCAGCACAATATGGTCGTGc > 1:467652/1‑68 (MQ=255)
aGTGATTTGCTGGATGAATGGGCGAAAGGAAACGTTCGTCAGCAACGAGCAGCACAATATGGTCGTGc > 1:2869092/1‑68 (MQ=255)
aGTGATTTGCTGGATGAATGGGCGAAAGGAAACGTTCGTCAGCAACGAGCAGCACAATATGGTCGTGc > 1:3133461/1‑68 (MQ=255)
aGTGATTTGCTGGATGAATGGGCGAAAGGAAACGTTCGTCAGCAACGAGCAGCACAATATGGTCGTGc > 1:3275083/1‑68 (MQ=255)
aGTGATTTGCTGGATGAATGGGCGAAAGGAAACGTTCGTCAGCAACGAGCAGCACAATATGGTCGTGc > 1:3408152/1‑68 (MQ=255)
aGTGATTTGCTGGATGAATGGGCGAAAGGAAACGTTCGTCAGCAACGAGCAGCACAATATGGTCGTGc > 1:3311802/1‑68 (MQ=255)
aGTGATTTGCTGGATGAATGGGCGAAAGGAAACGTTCGTCAGCAACGAGCAGCACAATATGGTCGTGc > 1:1464410/1‑68 (MQ=255)
aGTGATTTGCTGGATGAATGGGCGAAAGGAAACGTTCGTCAGCAACGAGCAGCACAATATGGTCGTGc > 1:117198/1‑68 (MQ=255)
aGTGATTTGCTGGATGAATGGGCGAAAGGAAACGTTCGTCAGCAACGAGCAGCACAATATGGTCGTGc > 1:946968/1‑68 (MQ=255)
aTTTGCTGGATGAATGGGCGAAAGGAAACGTTCGTCAGCAACGAGCAGCACAATATGGTCGTGCTTTa > 1:2528403/1‑68 (MQ=255)
aTTTGCTGGATGAATGGGCGAAAGGAAACGTTCGTCAGCAACGAGCAGCACAATATGGTCGTGCTTTAc > 1:1682529/1‑69 (MQ=255)
aTTTGCTGGATGAATGGGCGAAAGGAAACGTTCGTCAGCAACGAGCAGCACAATATGGTCGTGCTTTAc > 1:793950/1‑69 (MQ=255)
aTTTGCTGGATGAATGGGCGAAAGGAAACGTTCGTCAGCAACGAGCAGCACAATATGGTCGTGCTTTAc > 1:3407371/1‑69 (MQ=255)
aTTTGCTGGATGAATGGGCGAAAGGAAACGTTCGTCAGCAACGAGCAGCACAATATGGTCGTGCTTTAc > 1:3034431/1‑69 (MQ=255)
tttGCTGGATTAATGGGCGAAAGGAAACGTTCGTCAGCAACGAGCGGCGCaa < 1:446250/52‑1 (MQ=255)
tttGCTGGATGAATGGGCGAAAGGAAAGGTTCGTCAGCAACGAGCGGCGCaa < 1:2277300/52‑1 (MQ=38)
tttGCTGGATGAATGGGCGAAAGGAAACGTTCGTCAGCAACGAGCGGCGCaa < 1:544176/52‑1 (MQ=39)
tttGCTGGATGAATGGGCGAAAGGAAACGTTCGTCAGCAACGAGCGGCGCaa < 1:3109176/52‑1 (MQ=39)
tttGCTGGATGAATGGGCGAAAGGAAACGTTCGTCAGCAACGAGCGGCGCaa < 1:42834/52‑1 (MQ=39)
tttGCTGGATGAATGGGCGAAAGGAAACGTTCGTCAGCAACGAGCGGCGCaa < 1:1392642/52‑1 (MQ=39)
tttGCTGGATGAATGGGCGAAAGGAAACGTTCGTCAGCAACGAGCGGCGCaa < 1:3000398/52‑1 (MQ=39)
tttGCTGGATGAATGGGCGAAAGGAAACGTTCGTCAGCAACGAGCGGCGCaa < 1:449832/52‑1 (MQ=39)
tttGCTGGATGAATGGGCGAAAGGAAACGTTCGTCAGCAACGAGCGGCGCaa < 1:1327358/52‑1 (MQ=39)
tttGCTGGATGAATGGGCGAAAGGAAACGTTCGTCAGCAACGAGCGGCGCaa < 1:373486/52‑1 (MQ=39)
tttGCTGGATGAATGGGCGAAAGGAAACGTTCGTCAGCAACGAGCGGCGCaa < 1:1216178/52‑1 (MQ=39)
tttGCTGGATGAATGGGCGAAAGGAAACGTTCGTCAGCAACGAGCGGCGCaa < 1:194365/52‑1 (MQ=39)
tttGCTGGATGAATGGGCGAAAGGAAACGTTCGTCAGCAACGAGCGGCGCaa < 1:718096/52‑1 (MQ=39)
tttGCTGGATGAATGGGCGAAAGGAAACGTTCGTCAGCAACGAGCGGCGCaa < 1:1663148/52‑1 (MQ=39)
aaTGGGCGAAAGGAAACGTTCGTCAGCAACGAGCAGCACaa < 1:395444/41‑1 (MQ=255)
aaTGGGCGAAAGGAAACGTTCGTCAGCAACGAGCAGCACaa < 1:887192/41‑1 (MQ=255)
aaTGGGCGAAAGGAAACGTTCGTCAGCAACGAGCAGCACaa < 1:1629352/41‑1 (MQ=255)
aaTGGGCGAAAGGAAACGTTCGTCAGCAACGAGCAGCACaa < 1:1521010/41‑1 (MQ=255)
tGGGCGAAAGGAAACGTTCGTCAGCAACGAGCGGCGCaa < 1:533255/39‑1 (MQ=38)
tGGGCGAAAGGAAACGTTCGTCAGCAACGAGCAGCACAATATGGTCGTGCTTTACAGGCGATGGAAGc < 1:1554254/68‑1 (MQ=255)
tGGGCGAAAGGAAACGTTCGTCAGCAACGAGCAGCACAATATGGTCGTGCTTTACAGGCGATGGAAGc < 1:948840/68‑1 (MQ=255)
tGGGCGAAAGGAAACGTTCGTCAGCAACGAGCAGCACAATATGGTCGTGCTTTACAGGCGATGGAAGc < 1:3373816/68‑1 (MQ=255)
tGGGCGAAAGGAAACGTTCGTCAGCAACGAGCAGCACAATATGGTCGTGCTTTACAGGCGATGGAAGc < 1:1482523/68‑1 (MQ=255)
tGGGCGAAAGGAAACGTTCGTCAGCAACGAGCAGCACAATATGGTCGTGCTTTACAGGCGATGGAAGc < 1:2421348/68‑1 (MQ=255)
tGGGCGAAAGGAAACGTTCGTCAGCAACGAGCAGCACAATATGGTCGTGCTTTACAGGCGATGGAAGc < 1:185702/68‑1 (MQ=255)
tGGGCGAAAGGAAACGTTCGTCAGCAACGAGCAGCACAATATGGTCGTGCTTTACAGGCGATGGAAGc < 1:2352120/68‑1 (MQ=255)
tGGGCGAAAGGAAACGTTCGTCAGCAACGAGCAGCACAATATGGTCGTGCTTTACAGGCGATGGAAGc < 1:575348/68‑1 (MQ=255)
tGGGCGAAAGGAAACGTTCGTCAGCAACGAGCAGCACAATATGGTCCTGCTTTACAGGCGATGGAAGc < 1:743304/68‑1 (MQ=255)
gggCGAAAGGAAACGTTCGTCAGCAACGAGCAGCACAATATGGTCGTGCTTTACAGGCGATGGAAGcc < 1:504734/68‑1 (MQ=255)
ggCGAAAGGAAACGTTCGTCAGCAACGAGCAGCACAATATGGTCGTGCTTTACAGGCGATGGAAGcc > 1:3032425/1‑67 (MQ=255)
ggCGAAAGGAAACGTTCGTCAGCAACGAGCAGCACAATATGGTCGTGCTTTACAGGCGATGGAAGcc > 1:3206953/1‑67 (MQ=255)
ggCGAAAGGAAACGTTCGTCAGCAACGAGCAGCACAATATGGTCGTGCTTTACAGGCGATGGAAGcc > 1:660747/1‑67 (MQ=255)
ggCGAAAGGAAACGTTCGTCAGCAACGAGCAGCACAATATGGTCGTGCTTTACAGGCGATGGAAGCCa > 1:2620385/1‑68 (MQ=255)
ggCGAAAGGAAACGTTCGTCAGCAACGAGCAGCACAATATGGTCGTGCTTTACAGGCGATGGAAGCCa > 1:1747165/1‑68 (MQ=255)
ggCGAAAGGAAACGTTCGTCAGCAACGAGCAGCACAATATGGTCGTGCTTTACAGGCGATGGAAGCCa > 1:713983/1‑68 (MQ=255)
ggCGAAAGGAAACGTTCGTCAGCAACGAGCAGCACAATATGGTCGTGCTTTACAGGCGATGGAAGCCa > 1:3283894/1‑68 (MQ=255)
ggCGAAAGGAAACGTTCGTCAGCAACGAGCAGCACAATATGGTCGTGCTTTACAGGCGATGGAAGCCa > 1:3147824/1‑68 (MQ=255)
ggCGAAAGGAAACGTTCGTCAGCAACGAGCAGCACAATATGGTCGTGCTTTACAGGCGATGGAAGCCa > 1:675829/1‑68 (MQ=255)
ggCGAAAGGAAACGTTCGTCAGCAACGAGCAGCACAATATGGTCGTGCTTTACAGGCGATGGAAGCCa > 1:2127483/1‑68 (MQ=255)
ggCGAAAGGAAACGTTCGTCAGCAACGAGCAGCACAATATGGTCGTGCTTTACAGGCGATGGAAGCCa > 1:3094390/1‑68 (MQ=255)
ggCGAAAGGAAACGTTCGTCAGCAACGAGCAGCACAATATGGTCGTGCTTTACAGGCGATGGAAGCCa > 1:1410285/1‑68 (MQ=255)
ggCGAAAGGAAACGTTCGTCAGCAACGAGCAGCACAATATGGTCGTGCTTTACAGGCGATGGAAGCCa > 1:3028253/1‑68 (MQ=255)
ggCGAAAGGAAACGTTCGTCAGCAACGAGCAGCACAATATGGTCGTGCTTTACAGGCGATGGAAGCCa > 1:2480707/1‑68 (MQ=255)
gAAAGGAAACGTTCGTCAGCAACGAGCAGCACAATATg < 1:1361914/38‑1 (MQ=255)
caaGGAAACGTTCGTCAGCAACGAGCGGCGCAATATGGTCGTGCTTTACAGGCGa > 1:2542593/2‑55 (MQ=255)
aaaGGAAACGTTCGTCAGCAACGAGCGGCGCAATATGGTCGTGCTTTACAGGc > 1:1017806/1‑53 (MQ=255)
aaaGGAAACGTTCGTCAGCAACGAGCGGCGCAATATGGTCGTGCTTTACAGGCg > 1:3119058/1‑54 (MQ=255)
aaaGGAAACGTTCGTCAGCAACGAGCGGCGCAATATGGTCGTGCTTTACAGGCGa > 1:851908/1‑55 (MQ=255)
aaaGGAAACGTTCGTCAGCAACGAGCGGCGCAATATGGTCGTGCTTTACAGGCGa > 1:1836550/1‑55 (MQ=255)
aaaGGAAACGTTCGTCAGCAACGAGCGGCGCAATATGGTCGTGCTTTACAGGCGa > 1:2098132/1‑55 (MQ=255)
aaaGGAAACGTTCGTCAGCAACGAGCGGCGCAATATGGTCGTGCTTTACAGGCGa > 1:1438156/1‑55 (MQ=255)
aaaGGAAACGTTCGTCAGCAACGAGCGGCGCAATATGGTCGTGCTTTACAGGCGa > 1:2159768/1‑55 (MQ=255)
aaaGGAAACGTTCGTCAGCAACGAGCGGCGCAATATGGTCGTGCTTTACAGGCGa > 1:2276024/1‑55 (MQ=255)
aaaGGAAACGTTCGTCAGCAACGAGCGGCGCAATATGGTCGTGCTTTACAGGCGa > 1:832001/1‑55 (MQ=255)
aaaGGAAACGTTCGTCAGCAACGAGCGGCGCAATATGGTCGTGCTTTACAGGCGa > 1:2749832/1‑55 (MQ=255)
aaaGGAAACGTTCGTCAGCAACGAGCGGCGCAATATGGTCGTGCTTTACAGGCGa > 1:329835/1‑55 (MQ=255)
aaaGGAAACGTTCGTCAGCAACGAGCGGCGCAATATGGTCGTGCTTTACAGGCGa > 1:1015542/1‑55 (MQ=255)
aGGAAACGTTCGTCAGCAACGAGCAGCACAATATGTTCGTGCTTTACAGGCGATGGAAGCCaataaat > 1:1213304/1‑68 (MQ=255)
aGGAAACGTTCGTCAGCAACGAGCAGCACAATATGGTCGTGCTTTACAGGCGATGGAAGCCaataaat > 1:2865632/1‑68 (MQ=255)
cAACGAGCGGCGCAATATGGTCGTGCTTTACAGGCGATGGAAGCCAATAAATACGACGAGGCGCGTaa < 1:588481/68‑1 (MQ=255)
cAACGAGCGGCGCAATATGGTCGTGCTTTACAGGCGATGGAAGCCAATAAATACGACGAGGCGCGTaa < 1:1312624/68‑1 (MQ=255)
cAACGAGCGGCGCAATATGGTCGTGCTTTACAGGCGATGGAAGCCAATAAATACGACGAGGCGCGTaa < 1:1420848/68‑1 (MQ=255)
cAACGAGCGGCGCAATATGGTCGTGCTTTACAGGCGATGGAAGCCAATAAATACGACGAGGCGCGTaa < 1:1118043/68‑1 (MQ=255)
cAACGAGCGGCGCAATATGGTCGTGCTTTACAGGCGATGGAAGCCAATAAATACGACGAGGCGCGTaa < 1:1726545/68‑1 (MQ=255)
cAACGAGCGGCGCAATATGGTCGTGCTTTACAGGCGATGGAAGCCAATAAATACGACGAGGCGCGTaa < 1:1927835/68‑1 (MQ=255)
cAACGAGCGGCGCAATATGGTCGTGCTTTACAGGCGATGGAAGCCAATAAATACGACGAGGCGCGTaa < 1:2042203/68‑1 (MQ=255)
cAACGAGCGGCGCAATATGGTCGTGCTTTACAGGCGATGGAAGCCAATAAATACGACGAGGCGCGTaa < 1:339969/68‑1 (MQ=255)
cAACGAGCGGCGCAATATGGTCGTGCTTTACAGGCGATGGAAGCCAATAAATACGACGAGGCGCGTaa < 1:2142919/68‑1 (MQ=255)
cAACGAGCGGCGCAATATGGTCGTGCTTTACAGGCGATGGAAGCCAATAAATACGACGAGGCGCGTaa < 1:2388215/68‑1 (MQ=255)
cAACGAGCGGCGCAATATGGTCGTGCTTTACAGGCGATGGAAGCCAATAAATACGACGAGGCGCGTaa < 1:2668791/68‑1 (MQ=255)
cAACGAGCGGCGCAATATGGTCGTGCTTTACAGGCGATGGAAGCCAATAAATACGACGAGGCGCGTaa < 1:3040052/68‑1 (MQ=255)
cAACGAGCGGCGCAATATGGTCGTGCTTTACAGGCGATGGAAGCCAATAAATACGACGAGGCGCGTaa < 1:3067843/68‑1 (MQ=255)
cAACGAGCGGCGCAATATGGTCGTGCTTTACAGGCGATGGAAGCCAATAAATACGACGAGGCGCGTaa < 1:3420235/68‑1 (MQ=255)
cAACGAGCGGCGCAATATGGTCGTGCTTTACAGGCGATGGAAGCCAATAAATACGACGAGGCGCGTaa < 1:3357923/68‑1 (MQ=255)
cAACGAGCGGCGCAATATGGTCGTGCTTTACAGGCGATGCAAGCCAATAAATACGACGAGGCGCGTaa < 1:1419343/68‑1 (MQ=255)
|
AGCTCACCAGTGATTTGCTGGATGAATGGGCGAAAGGAAACGTTCGTCAGCAACGAGCAGCACAATATGGTCGTGCTTTACAGGCGATGGAAGCCAATAAATACGACGAGGCGCGTAA > minE/1649651‑1649768
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
