Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,658,013 |
G→A |
45.8% |
L71L (CTG→CTA) |
ppx → |
exopolyphosphatase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,658,013 | 0 | G | A | 45.8%
| 10.6
/ 57.7
| 72 | L71L (CTG→CTA) | ppx | exopolyphosphatase |
| Reads supporting (aligned to +/- strand): ref base G (31/8); new base A (22/11); total (53/19) |
| Fisher's exact test for biased strand distribution p-value = 2.86e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01 |
TAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATAC > minE/1657962‑1658072
|
taataTGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACa > 1:3356807/1‑69 (MQ=255)
taataTGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACa > 1:471696/1‑69 (MQ=255)
taataTGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACa > 1:1999967/1‑69 (MQ=255)
taataTGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACa > 1:2357037/1‑69 (MQ=255)
taataTGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACa > 1:402269/1‑69 (MQ=255)
taataTGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACa > 1:375290/1‑69 (MQ=255)
taataTGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACa > 1:3242264/1‑69 (MQ=255)
taataTGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCCGTTTGCCGAACGGCTACa > 1:395672/1‑69 (MQ=255)
tatGTTGAGTGAAGAGGCAATGACGCGCGGTTTGAACTGTCTGTCGCTATTTGCCg > 1:3252681/1‑56 (MQ=255)
tatGTTGAGTGAAGAGGCAATGACGCGCGGTTTGAACTGTCTGTCGCTATTTGCCGAAAGGCTACAAgg > 1:3086630/1‑69 (MQ=255)
tatGTTGAGTGAAGAGGCAATGACGCGCGGTTTGAACTGTCTGTCGCTATTTGCCGAAAGGCTACAAgg > 1:3002628/1‑69 (MQ=255)
tatGTTGAGTGAAGAGGCAATGACGCGCGGTTTGAACTGTCTGTCGCTATTTGCCGAAAGGCTACAAgg > 1:2932277/1‑69 (MQ=255)
tatGTTGAGTGAAGAGGCAATGACGCGCGGTTTGAACTGTCTGTCGCTATTTGCCGAAAGGCTACAAgg > 1:2792530/1‑69 (MQ=255)
tatGTTGAGTGAAGAGGCAATGACGCGCGGTTTGAACTGTCTGTCGCTATTTGCCGAAAGGCTACAAgg > 1:2428471/1‑69 (MQ=255)
tatGTTGAGTGAAGAGGCAATGACGCGCGGTTTGAACTGTCTGTCGCTATTTGCCGAAAGGCTACAAgg > 1:2419473/1‑69 (MQ=255)
tatGTTGAGTGAAGAGGCAATGACGCGCGGTTTGAACTGTCTGTCGCTATTTGCCGAAAGGCTACAAgg > 1:34805/1‑69 (MQ=255)
tatGTTGAGTGAAGAGGCAATGACGCGCGGTTTGAACTGTCTGTCGCTATTTGCCGAAAGGCTACAAgg > 1:2342997/1‑69 (MQ=255)
tatGTTGAGTGAAGAGGCAATGACGCGCGGTTTGAACTGTCTGTCGCTATTTGCCGAAAGGCTACAAgg > 1:335010/1‑69 (MQ=255)
tatGTTGAGTGAAGAGGCAATGACGCGCGGTTTGAACTGTCTGTCGCTATTTGCCGAAAGGCTACAAgg > 1:3410855/1‑69 (MQ=255)
tatGTTGAGTGAAGAGGCAATGACGCGCGGTTTGAACTGTCTGTCGCTATTTGCCGAAAGGCTACAAgg > 1:102017/1‑69 (MQ=255)
tatGTTGAGTGAAGAGGCAATGACGCGCGGTTTGAACTGTCTGTCGCTATTTGCCGAAAGGCTACAAgg > 1:2018854/1‑69 (MQ=255)
tatGTTGAGTGAAGAGGCAATGACGCGCGGTTTGAACTGTCTGTCGCTATTTGCCGAAAGGCTACAAgg > 1:1997557/1‑69 (MQ=255)
tatGTTGAGTGAAGAGGCAATGACGCGCGGTTTGAACTGTCTGTCGCTATTTGCCGAAAGGCTACAAgg > 1:760350/1‑69 (MQ=255)
tatGTTGAGTGAAGAGGCAATGACGCGCGGTTTGAACTGTCTGTCGCTATTTGCCGAAAGGCTACAAgg > 1:644237/1‑69 (MQ=255)
tatGTTGAGTGAAGAGGCAATGACGCGCGGTTTGAACTGTCTGTCGCTATTTGCCGAAAGGCTACAAgg > 1:1206064/1‑69 (MQ=255)
tatGTTGAGTGAAGAGGCAATGACGCGCGGTTTGAACTGTCTGTCGCTATTTGCCGAAAGGCTACAAgg > 1:1628431/1‑69 (MQ=255)
tatGTTGAGTGAAGAGGCAATGACGCGCGGTTTGAACTGTCTGTCGCTATTTGCCGAAAGGCTACAAgg > 1:1603556/1‑69 (MQ=255)
tatGTTGAGTGAAGAGGCAATGACGCGCGGTTTGAACTGTCTGTCGCTATTTGCCGAAAGGCTACAAgg > 1:1353672/1‑69 (MQ=255)
tatGTTGAGTGAAGAGGCAATGACGCGCGGTTTGAACTGTCTGTCGCTATTTGCCGAAAGGCTACAAgg > 1:535052/1‑69 (MQ=255)
tatGTTGAGTGAAGAGGCAATGACGCGCGGTTTGAACTGTCTGTCGCTATTTGCCGAAAGGCTACAAgg > 1:1533218/1‑69 (MQ=255)
gTGAAGAGGCAATGACGCGCGGTTTGAACTGTCTGTCGCTATTTGCCGAAAGGCTACAAGGGTTTtct < 1:2099548/68‑1 (MQ=255)
gTGAAGAGGCAATGACGCGCGGTTTGAACTGTCTGTCGCTATTTGCCGAAAGGCTACAAGGGTTTtct < 1:3353265/68‑1 (MQ=255)
gTGAAGAGGCAATGACGCGCGGTTTGAACTGTCTGTCGCTATTTGCCGAAAGGCTACAAGGGTTTtct < 1:2777999/68‑1 (MQ=255)
gTGAAGAGGCAATGACGCGCGGTTTGAACTGTCTGTCGCTATTTGCCGAAAGGCTACAAGGGTTTtct < 1:2765239/68‑1 (MQ=255)
gTGAAGAGGCAATGACGCGCGGTTTGAACTGTCTGTCGCTATTTGCCGAAAGGCTACAAGGGTTTtct < 1:2668322/68‑1 (MQ=255)
gTGAAGAGGCAATGACGCGCGGTTTGAACTGTCTGTCGCTATTTGCCGAAAGGCTACAAGGGTTTtct < 1:416021/68‑1 (MQ=255)
gTGAAGAGGCAATGACGCGCGGTTTGAACTGTCTGTCGCTATTTGCCGAAAGGCTACAAGGGTTTtct < 1:434141/68‑1 (MQ=255)
gTGAAGAGGCAATGACGCGCGGTTTGAACTGTCTGTCGCTATTTGCCGAAAGGCTACAAGGGTTTtct < 1:2381426/68‑1 (MQ=255)
gTGAAGAGGCAATGACGCGCGGTTTGAACTGTCTGTCGCTATTTGCCGAAAGGCTACAAGGGTTTtct < 1:46440/68‑1 (MQ=255)
gTGAAGAGGCAATGACGCGCGGTTTGAACTGTCTGTCGCTATTTGCCGAAAGGCTACAAGGGTTTtct < 1:513659/68‑1 (MQ=255)
gTGAAGAGGCAATGACGCGCGGTTTGAACTGTCTGTCGCTATTTGCCGAAAGGCTACAAGGGTTTtct < 1:2167437/68‑1 (MQ=255)
gCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAg > 1:1337172/1‑68 (MQ=255)
gCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGc > 1:561023/1‑69 (MQ=255)
gCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGc > 1:1213176/1‑69 (MQ=255)
gCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGc > 1:772720/1‑69 (MQ=255)
gACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTtctc > 1:869566/1‑56 (MQ=255)
gACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTtctc > 1:504314/1‑56 (MQ=255)
gACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTtctc > 1:1064244/1‑56 (MQ=255)
gACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTtctc > 1:3272575/1‑56 (MQ=255)
gACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTtctc > 1:1153990/1‑56 (MQ=255)
gACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTtctc > 1:1176157/1‑56 (MQ=255)
gACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTtctc > 1:1198881/1‑56 (MQ=255)
gACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTtctc > 1:2376186/1‑56 (MQ=255)
gACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTtct > 1:814379/1‑55 (MQ=255)
gACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTg > 1:3152977/1‑69 (MQ=255)
gACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTg > 1:451110/1‑69 (MQ=255)
gACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTg > 1:611168/1‑69 (MQ=255)
gACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTg > 1:483980/1‑69 (MQ=255)
gACGCGCGGTTTAAACTGTCTGTCGATGTTTGCCGAACGGCTACAAGGGTTTtctc > 1:2035195/1‑56 (MQ=255)
gcgcgGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGtat < 1:2307802/69‑1 (MQ=255)
gcgcgGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGtat < 1:1127120/69‑1 (MQ=255)
cgGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGt < 1:1729977/69‑1 (MQ=255)
ggTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGtt < 1:633059/69‑1 (MQ=255)
ggTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGtt < 1:761084/69‑1 (MQ=255)
ggTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGtt < 1:2987145/69‑1 (MQ=255)
gTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGtt < 1:3407798/68‑1 (MQ=255)
gTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGtt < 1:2934072/68‑1 (MQ=255)
tAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTg > 1:138203/1‑58 (MQ=255)
tAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTg > 1:466326/1‑58 (MQ=255)
tAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTg > 1:2444849/1‑58 (MQ=255)
tAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTg > 1:2555791/1‑58 (MQ=255)
tctgtcGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATAc > 1:1492696/1‑69 (MQ=255)
|
TAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATAC > minE/1657962‑1658072
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
