Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,660,974 |
C→T |
24.8% |
A217A (GCG→GCA) |
yfgF ← |
predicted inner membrane protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,660,974 | 0 | C | T | 24.8%
| 168.4
/ 47.2
| 132 | A217A (GCG→GCA) | yfgF | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (53/46); new base T (14/19); total (67/65) |
| Fisher's exact test for biased strand distribution p-value = 3.17e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.74e-01 |
Only 100 of 132 total aligned reads displayed.
TTTTCATTTAACGGCATGCATAACAGCAACAATAAAGCACCTAATGCCAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGCATCAACCTGCTGTTTTAATTGCGAATAATAGCTA > minE/1660915‑1661034
|
ttttCATTTAACGGCATGCATAACAGCAACAATAAAGCACCTAATGCCAGTAGCCAGATTGCGa < 1:3050008/64‑1 (MQ=255)
ttttCATTTAACGGCATGCATAACAGCAACAATAAAGCACCTAATGCCAGTAGCCAGATTGCGa < 1:1712178/64‑1 (MQ=255)
ttttCATTTAACGGCATGCATAACAGCAACAATAAAGCACCTAATGCCAGTAGCCAGATTGCGa < 1:1810507/64‑1 (MQ=255)
ttttCATTTAACGGCATGCATAACAGCAACAATAAAGCACCTAATGCCAGTAGCCAGATTGCGa < 1:739885/64‑1 (MQ=255)
ttttCATTTAACGGCATGCATAACAGCAACAATAAAGCACCTAATGCCAGTAGCCAGATTGCGa < 1:741080/64‑1 (MQ=255)
ttttCATTTAACGGCATGCATAACAGCAACAATAAAGCACCTAATGCCAGTAGCCAGATTGCGa < 1:1825925/64‑1 (MQ=255)
ttttCATTTAACGGCATGCATAACAGCAACAATAAAGCACCTAATGCCAGTAGCCAGATTGCGa < 1:598092/64‑1 (MQ=255)
ttttCATTTAACGGCATGCATAACAGCAACAATAAAGCACCTAATGCCAGTAGCCAGATTGCGa < 1:496725/64‑1 (MQ=255)
ttttCATTTAACGGCATGCATAACAGCAACAATAAAGCACCTAATGCCAGTAGCCAGATTGCGa < 1:413019/64‑1 (MQ=255)
ttttCATTTAACGGCATGCATAACAGCAACAATAAAGCACCTAATGCCAGTAGCCAGATTGCGa < 1:358847/64‑1 (MQ=255)
ttttCATTTAACGGCATGCATAACAGCAACAATAAAGCACCTAATGCCAGTAGCCAGATTGCGa < 1:347161/64‑1 (MQ=255)
ttttCATTTAACGGCATGCATAACAGCAACAATAAAGCACCTAATGCCAGTAGCCAGATTGCGa < 1:2706014/64‑1 (MQ=255)
ttttCATTTAACGGCATGCATAACAGCAACAATAAAGCACCTAATGCCAGTAGCCAGATTGCGa < 1:2767580/64‑1 (MQ=255)
ggCATGCATAACAGCAATAATAAAGCACCTAATGCCAGTAGCCAGAGCGCGAACTCTTTTTTGGTGAc < 1:1667603/68‑1 (MQ=255)
tgcatAACAGCAACAATAAAGCACCTAATGCCAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTgg < 1:2281853/69‑1 (MQ=255)
tgcatAACAGCAACAATAAAGCACCTAATGCCAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTgg < 1:402822/69‑1 (MQ=255)
tgcatAACAGCAACAATAAAGCACCTAATGCCAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTgg < 1:3353533/69‑1 (MQ=255)
tgcatAACAGCAACAATAAAGCACCTAATGCCAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTgg < 1:1805614/69‑1 (MQ=255)
tgcatAACAGCAACAATAAAGCACCTAATGCCAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTgg < 1:1017782/69‑1 (MQ=255)
tgcatAACAGCAACAATAAAGCACCTAATGCCAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTgg < 1:1636422/69‑1 (MQ=255)
tgcatAACAGCAACAATAAAGCACCTAATGCCAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTgg < 1:1144348/69‑1 (MQ=255)
tgcatAACAGCAACAATAAAGCACCTAATGCCAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTgg < 1:1440342/69‑1 (MQ=255)
catAACAGCAACAATAAAGCACCTAATGCCAGTAGCCAGAGCGCGAACTCtttttt < 1:248219/56‑1 (MQ=255)
catAACAGCAACAATAAAGCACCTAATGCCAGTAGCCAGAGCGCGAACTCtttttt < 1:2372782/56‑1 (MQ=255)
catAACAGCAACAATAAAGCACCTAATGCCAGTAGCCAGAGCGCGAACTCtttttt < 1:2937113/56‑1 (MQ=255)
catAACAGCAACAATAAAGCACCTAATGCCAGTAGCCAGAGCGCGAACTCtttttt < 1:2237673/56‑1 (MQ=255)
catAACAGCAACAATAAAGCACCTAATGCCAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGc > 1:675683/1‑68 (MQ=255)
catAACAGCAACAATAAAGCACCTAATGCCAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGc > 1:2838105/1‑68 (MQ=255)
catAACAGCAACAATAAAGCACCTAATGCCAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGc > 1:1125128/1‑68 (MQ=255)
catAACAGCAACAATAAAGCACCTAATGCCAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGc > 1:253517/1‑68 (MQ=255)
catAACAGCAACAATAAAGCACCTAATGCCAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGc > 1:1209412/1‑68 (MQ=255)
catAACAGCAACAATAAAGCACCTAATGCCAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGc > 1:2296602/1‑68 (MQ=255)
catAACAGCAACAATAAAGCACCTAATGCCAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGc > 1:2884247/1‑68 (MQ=255)
catAACAGCAACAATAAAGCACCTAATGCCAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGc > 1:2151139/1‑68 (MQ=255)
catAACAGCAACAATAAAGCACCTAATGCCAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGc > 1:1275840/1‑68 (MQ=255)
catAACAGCAACAATAAAGCACCTAATGCCAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGc > 1:2008165/1‑68 (MQ=255)
catAACAGCAACAATAAAGCACCTAATGCCAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGc > 1:774824/1‑68 (MQ=255)
catAACAGCAACAATAAAGCACCTAATGCCAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGc > 1:611472/1‑68 (MQ=255)
catAACAGCAACAATAAAGCACCTAATGCCAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGc > 1:3108693/1‑68 (MQ=255)
atAACAGCAACAATAAAGCACCTAATGCCAGTAGCCAGATTGCGa < 1:673590/45‑1 (MQ=255)
aaCAGCAACAATAAAGCACCTAATGCCAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGCATCa < 1:2881877/69‑1 (MQ=255)
aaCAGCAACAATAAAGCACCTAATGCCAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGCATCa < 1:151686/69‑1 (MQ=255)
aaCAGCAACAATAAAGCACCTAATGCCAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGCATCa < 1:1736476/69‑1 (MQ=255)
aCAGCAACAATAAAGCACCTAATGCCAGTAGCCAGAGCGCGAACTCttttt > 1:2822347/1‑51 (MQ=255)
aCAGCAACAATAAAGCACCTAATGCCAGTAGCCAGAGCGCGAACTCttttt > 1:2083692/1‑51 (MQ=255)
aCAGCAACAATAAAGCACCTAATGCCAGTAGCCAGAGCGCGAACTCttttt > 1:1008702/1‑51 (MQ=255)
aCAGCAACAATAAAGCACCTAATGCCAGTAGCCAGAGCGCGAACTCttttt > 1:1030814/1‑51 (MQ=255)
aCAGCAACAATAAAGCACCTAATGCCAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGCATCaa > 1:1611819/1‑69 (MQ=255)
aCAGCAACAATAAAGCACCTAATGCCAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGCATCaa > 1:2879874/1‑69 (MQ=255)
aCAGCAACAATAAAGCACCTAATGCCAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGCATCa > 1:2415306/1‑68 (MQ=255)
aCAGCAACAATAAAGCACCTAATGCCAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGCATCa < 1:865453/68‑1 (MQ=255)
aCAGCAACAATAAAGCACCTAATGCCAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGCATCa < 1:2909898/68‑1 (MQ=255)
gCAACAATAAAGCACCTAATGCCAGTAGCCAGAGCGCGAACTCTTTTTTGCTGACTTTGGCATCAACct > 1:3161847/1‑69 (MQ=255)
tAAAGCACCTAATGCCAGTAGCCAGATTGCGAACTCTTTTTTGGTAACCTTGGCATCAACCTGCTGtt > 1:618787/1‑68 (MQ=255)
tAAAGCACCTAATGCCAGTAGCCAGATTGCGAACTCTTTTTTGGTAACCTTGGCATCAACCTGCTGtt > 1:3058766/1‑68 (MQ=255)
tAAAGCACCTAATGCCAGTAGCCAGATTGCGAACTCTTTTTTGGTAACCTTGGCATCAACCTGCTGtt > 1:1233808/1‑68 (MQ=255)
tAAAGCACCTAATGCCAGAAGCCAGATTGCGAACTCTTTTTTGGt > 1:572495/1‑45 (MQ=255)
aaaGCACCTAATGCCAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGCATCAACCTGCTGtttt < 1:2369356/69‑1 (MQ=255)
aaaGCACCTAATGCCAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGCATCAACCTGCTGtttt < 1:3204566/69‑1 (MQ=255)
aaaGCACCTAATGCCAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGCATCAACCTGCTGtttt < 1:1073442/69‑1 (MQ=255)
aaaGCACCTAATGCCAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGCATCAACCTGCTGtttt < 1:488819/69‑1 (MQ=255)
aaaGCACCTAATGCCAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGCATCAACCTGCTGtttt < 1:1985655/69‑1 (MQ=255)
aaaGCACCTAATGCCAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGCATCAACCTGCTGtttt < 1:1394112/69‑1 (MQ=255)
aaaGCACCTAATGCCAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGCATCAACCTGCTGtttt < 1:698414/69‑1 (MQ=255)
aaaGCACCTAATGCCAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGCATCAACCTGCTGtttt < 1:1674663/69‑1 (MQ=255)
aaGCACCTAATGCCAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGCATCAACCTGCTGtttt < 1:3347195/68‑1 (MQ=255)
aaTGCCAGTAGCCAGATTGCGAACTCTTTTTTGGTAACCTTGGCATCAACctgct > 1:3421934/1‑55 (MQ=255)
aaTGCCAGTAGCCAGATTGCGAACTCTTTTTTGGTAACCTTGGCATCAACctgct > 1:97728/1‑55 (MQ=255)
aaTGCCAGTAGCCAGATTGCGAACTCTTTTTTGGTAACCTTGGCATCAACctgct > 1:2808050/1‑55 (MQ=255)
aaTGCCAGTAGCCAGATTGCGAACTCTTTTTTGGTAACCTTGGCATCAACctgct > 1:1891365/1‑55 (MQ=255)
aaTGCCAGTAGCCAGATTGCGAACTCTTTTTTGGTAACCTTGGCATCAACctgct > 1:276299/1‑55 (MQ=255)
aaTGCCAGTAGCCAGATTGCGAACTCTTTTTTGGTAACCTTGGCATCAACctgct > 1:2346295/1‑55 (MQ=255)
aaTGCCAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGCATCAACCTGCTGTTTTAATTGCg > 1:1435460/1‑67 (MQ=255)
aaTGCCAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGCATCAACCTGCTGTTTTAATTGCGaa < 1:1511451/69‑1 (MQ=255)
aaTGCCAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGCATCAACCTGCTGTTTTAATTGCGaa < 1:2830383/69‑1 (MQ=255)
aaTGCCAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGCATCAACCTGCTGTTTTAATTGCGaa < 1:3276285/69‑1 (MQ=255)
aaTGCCAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGCATCAACCTGCTGTTTTAATTGCGaa < 1:2111107/69‑1 (MQ=255)
aaTGCCAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGCATCAACCTGCTGTTTTAATTGCGa > 1:2672859/1‑68 (MQ=255)
aaTGCCAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGCATCAACCTGCTGTTTTAATTGCGa > 1:3430600/1‑68 (MQ=255)
aTGCCAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGCATCAACCTGCTGTTTTAATTGCGaa < 1:425764/68‑1 (MQ=255)
cAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGCa > 1:2954506/1‑40 (MQ=255)
cAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGCATCAACctgatg > 1:3160898/1‑51 (MQ=255)
cAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGCATCAACCTGCTGTTTTAATTGCGaataat > 1:770735/1‑68 (MQ=255)
cAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGCATCAACCTGCTGTTTTAATTGCGAataata > 1:1029704/1‑69 (MQ=255)
cAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGCATCAACCTGCTGTTTTAATTGCGAataata > 1:1707436/1‑69 (MQ=255)
cAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGCATCAACCTGCTGTTTTAATTGCGAataata > 1:189861/1‑69 (MQ=255)
gTAGCCAGAGCGAGAACTCTTTTTTGGTGACTTTgg < 1:184842/36‑1 (MQ=255)
gCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGCATc > 1:1999638/1‑37 (MQ=255)
gCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGCATCa > 1:3212930/1‑38 (MQ=255)
gCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGCATCa > 1:2481012/1‑38 (MQ=255)
gCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGCATCa > 1:2266237/1‑38 (MQ=255)
gCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGCATCa > 1:1548447/1‑38 (MQ=255)
gCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGCATCa > 1:2685427/1‑38 (MQ=255)
gCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGCATCa < 1:3036051/38‑1 (MQ=255)
gCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGCATCa > 1:14264/1‑38 (MQ=255)
gCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGCATCa > 1:1247457/1‑38 (MQ=255)
gCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGCATCAACCTGCTGTTTTAATTGCGAATAATAGCTa > 1:3307676/1‑68 (MQ=255)
gCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGCATCAACCTGCTGTTTTAATTGCGAATAATAGCTa > 1:2337709/1‑68 (MQ=255)
gCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGCATCAACCTGCTGTTTTAATTGCGAATAATAGCTa > 1:1342782/1‑68 (MQ=255)
gCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGCATCAACCTGCTGTTTTAATTGCGAATAATAGCTa > 1:2662690/1‑68 (MQ=255)
|
TTTTCATTTAACGGCATGCATAACAGCAACAATAAAGCACCTAATGCCAGTAGCCAGAGCGCGAACTCTTTTTTGGTGACTTTGGCATCAACCTGCTGTTTTAATTGCGAATAATAGCTA > minE/1660915‑1661034
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
