Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,665,995 |
T→A |
44.1% |
S254S (TCA→TCT) |
guaB ← |
IMP dehydrogenase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,665,995 | 0 | T | A | 44.1%
| 14.3
/ 62.6
| 59 | S254S (TCA→TCT) | guaB | IMP dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base T (26/7); new base A (8/18); total (34/25) |
| Fisher's exact test for biased strand distribution p-value = 4.39e-04 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.86e-01 |
AATTTGCAGATCCGGATATTTAGCACGGGTTTCACGGATACGTTGCAGTACACCTTCTGAGTGACCGTGGGAGGAGTCGATCAGCAGAACGTCAACGCCTGCGGCAACCAGCGCGTCAA > minE/1665938‑1666056
|
gaTTTGCAGGTCAGGATATTTAGCGCGAGTTTCACGGATACGCTGCAGAACACCTTCAGAGTGAcc > 1:997326/2‑66 (MQ=255)
gaTTTGCAGGTCAGGATATTTAGCGCGAGTTTCACGGATACGCTGCAGAACACCTTCAGAGTGAcc > 1:858483/2‑66 (MQ=255)
gaTTTGCAGGTCAGGATATTTAGCGCGAGTTTCACGGATACGCTGCAGAACACCTTCAGAGTGAcc > 1:485990/2‑66 (MQ=255)
gaTTTGCAGGTCAGGATATTTAGCGCGAGTTTCACGGATACGCTGCAGAACACCTTCAGAGTGAcc > 1:3211438/2‑66 (MQ=255)
gaTTTGCAGGTCAGGATATTTAGCGCGAGTTTCACGGATACGCTGCAGAACACCTTCAGAGTGAcc > 1:1356041/2‑66 (MQ=255)
gaTTTGCAGGTCAGGATATTTAGCGCGAGTTTCACGGATACGCTGCAGAACACCTTCAGAGTGAcc > 1:2878472/2‑66 (MQ=255)
gaTTTGCAGGTCAGGATATTTAGCGCGAGTTTCACGGATACGCTGCAGAACACCTTCAGAGTGAcc > 1:1834286/2‑66 (MQ=255)
gaTTTGCAGGTCAGGATATTTAGCGCGAGTTTCACGGATACGCTGCAGAACACCTTCAGAGTGAcc > 1:1704295/2‑66 (MQ=255)
ttGCAGATCCGGATATTTAGCACGGGTTTCACGGATACGTTGCAGTACACCTTCTGAGTGACCGTggg < 1:2114374/68‑1 (MQ=255)
ccGGATATTTAGCACGGGTTTCACGGATACGTTGCAGTACACCTTCTGAGTGACCGTGGGAGGAGTCg > 1:2786754/1‑68 (MQ=255)
tttAGCACGGGTTTCACGGATACGTTGCAGTACACCTTCTGAGTGACCGTGGGAGGAGTCGATcaggag > 1:2963779/1‑69 (MQ=255)
tttAGCACGGGTTTCACGGATACGTTGCAGTACACCTTCTGAGTGACCGTGGGAGGAGTCGATcagcag > 1:1066890/1‑69 (MQ=255)
tttAGCACGGGTTTCACGGATACGTTGCAGTACACCTTCTGAGTGACCGTGGGAGGAGTCGATcagcag > 1:612765/1‑69 (MQ=255)
tttAGCACGGGTTTCACGGATACGTTGCAGTACACCTTCTGAGTGACCGTGGGAGGAGTCGATcagcag > 1:378671/1‑69 (MQ=255)
tttAGCACGGGTTTCACGGATACGTTGCAGTACACCTTCTGAGTGACCGTGGGAGGAGTCGATcagcag > 1:367638/1‑69 (MQ=255)
tttAGCACGGGTTTCACGGATACGTTGCAGTACACCTTCTGAGTGACCGTGGGAGGAGTCGATcagcag > 1:2591836/1‑69 (MQ=255)
aCGGGTTTCACGGATACGTTGCAGTACACCTTCTGAGTGACCGTGGGAGGAGTCGATCAGCAGAACGTc < 1:3001855/69‑1 (MQ=255)
cGGGTTTCACGGATACGTTGCAGTACACCTTCTGAGTGACCGTGGGAGGAGTCGATCAGCAGAACGTc < 1:1158920/68‑1 (MQ=255)
aCGCTGCAGAACACCTTCAGAGTGACCGTGAGAGGAGTCGATCAGCAGAACGTCAACGCCTTCGGCAAc < 1:2369249/69‑1 (MQ=255)
aCGCTGCAGAACACCTTCAGAGTGACCGTGAGAGGAGTCGATCAGCAGAACGTCAACGCCTGCGGCAAc < 1:2529081/69‑1 (MQ=255)
aCGCTGCAGAACACCTTCAGAGTGACCGTGAGAGGAGTCGATCAGCAGAACGTCAACGCCTGCGGCAAc < 1:3001844/69‑1 (MQ=255)
aCGCTGCAGAACACCTTCAGAGTGACCGTGAGAGGAGTCGATCAGCAGAACGTCAACGCCTGCGGCAAc < 1:1350574/69‑1 (MQ=255)
aCGCTGCAGAACACCTTCAGAGTGACCGTGAGAGGAGTCGATCAGCAGAACGTCAACGCCTGCGGCAAc < 1:3014893/69‑1 (MQ=255)
aCGCTGCAGAACACCTTCAGAGTGACCGTGAGAGGAGTCGATCAGCAGAACGTCAACGCCTGCGGCAAc < 1:3068670/69‑1 (MQ=255)
aCGCTGCAGAACACCTTCAGAGTGACCGTGAGAGGAGTCGATCAGCAGAACGTCAACGCCTGCGGCAAc < 1:3071324/69‑1 (MQ=255)
aCGCTGCAGAACACCTTCAGAGTGACCGTGAGAGGAGTCGATCAGCAGAACGTCAACGCCTGCGGCAAc < 1:3092124/69‑1 (MQ=255)
aCGCTGCAGAACACCTTCAGAGTGACCGTGAGAGGAGTCGATCAGCAGAACGTCAACGCCTGCGGCAAc < 1:2635992/69‑1 (MQ=255)
aCGCTGCAGAACACCTTCAGAGTGACCGTGAGAGGAGTCGATCAGCAGAACGTCAACGCCTGCGGCAAc < 1:2606107/69‑1 (MQ=255)
aCGCTGCAGAACACCTTCAGAGTGACCGTGAGAGGAGTCGATCAGCAGAACGTCAACGCCTGCGGCAAc < 1:1248622/69‑1 (MQ=255)
aCGCTGCAGAACACCTTCAGAGTGACCGTGAGAGGAGTCGATCAGCAGAACGTCAACGCCTGCGGCAAc < 1:1648556/69‑1 (MQ=255)
aCGCTGCAGAACACCTTCAGAGTGACCGTGAGAGGAGTCGATCAGCAGAACGTCAACGCCTGCGGCAAc < 1:2058640/69‑1 (MQ=255)
aCGCTGCAGAACACCTTCAGAGTGACCGTGAGAGGAGTCGATCAGCAGAACGTCAACGCCTGCGGCAAc < 1:1897878/69‑1 (MQ=255)
aCGCTGCAGAACACCTTCAGAGTGACCGTGAGAGGAGTCGATCAGCAGAACGTCAACGCCTGCGGCAAc < 1:941699/69‑1 (MQ=255)
aCGCTGCAGAACACCTTCAGAGTGACCGTGAGAGGAGTCGATCAGCAGAACGTCAACGCCTGCGGCAAc < 1:1816757/69‑1 (MQ=255)
aCGCTGCAGAACACCTTCAGAGTGACCGTGAGAGGAGTCGATCAGCAGAACGTCAACGCCTGCGGCAAc < 1:1813324/69‑1 (MQ=255)
cAGAACACCTTCAGAGTGACCGTGAGAGGAGTCGATCAGCAGAACGTCAACGCCTGCGGCAAc < 1:3076647/63‑1 (MQ=255)
tACACCTTCTGAGTGACCGTGGGAGGAGTCGATCAGCAGAACGTCAACGCCTGCGGCAACCAGCGCGTc < 1:1276177/69‑1 (MQ=255)
tACACCTTCTGAGTGACCGTGGGAGGAGTCGATCAGCAGAACGTCAACGCCTGCGGCAACCAGCGCGTc < 1:2299277/69‑1 (MQ=255)
tACACCTTCTGAGTGACCGTGGGAGGAGTCGATCAGCAGAACGTCAACGCCTGCGGCAACCAGCGCGTc < 1:1871025/69‑1 (MQ=255)
acacCTTCTGAGTGACCGTGGGAGGAGTCGATCAGCAGAACGTCAACGCCTGCGGCAACCAGCGCGTc < 1:282002/68‑1 (MQ=255)
cacCTTCTGAGTGACCGTGGGAGGAGTCGATCAGCAGAACGt > 1:1081655/1‑42 (MQ=255)
cacCTTCTGAGTGACCGTGGGAGGAGTCGATCAGCAGAACGTCAACGCCTGCGGCAAc > 1:1018539/1‑58 (MQ=255)
cacCTTCTGAGTGACCGTGGGAGGAGTCGATCAGCAGAACGTCAACGCCTGCGGCAACCAGCGCGTCaa > 1:1220522/1‑69 (MQ=255)
cacCTTCTGAGTGACCGTGGGAGGAGTCGATCAGCAGAACGTCAACGCCTGCGGCAACCAGCGCGTCaa > 1:1654803/1‑69 (MQ=255)
cacCTTCTGAGTGACCGTGGGAGGAGTCGATCAGCAGAACGTCAACGCCTGCGGCAACCAGCGCGTCaa > 1:984878/1‑69 (MQ=255)
cacCTTCTGAGTGACCGTGGGAGGAGTCGATCAGCAGAACGTCAACGCCTGCGGCAACCAGCGCGTCaa > 1:945103/1‑69 (MQ=255)
cacCTTCTGAGTGACCGTGGGAGGAGTCGATCAGCAGAACGTCAACGCCTGCGGCAACCAGCGCGTCaa > 1:1650849/1‑69 (MQ=255)
cacCTTCTGAGTGACCGTGGGAGGAGTCGATCAGCAGAACGTCAACGCCTGCGGCAACCAGCGCGTCaa > 1:19404/1‑69 (MQ=255)
cacCTTCTGAGTGACCGTGGGAGGAGTCGATCAGCAGAACGTCAACGCCTGCGGCAACCAGCGCGTCaa > 1:1115660/1‑69 (MQ=255)
cacCTTCTGAGTGACCGTGGGAGGAGTCGATCAGCAGAACGTCAACGCCTGCGGCAACCAGCGCGTCaa > 1:2001039/1‑69 (MQ=255)
cacCTTCTGAGTGACCGTGGGAGGAGTCGATCAGCAGAACGTCAACGCCTGCGGCAACCAGCGCGTCaa > 1:1427856/1‑69 (MQ=255)
cacCTTCTGAGTGACCGTGGGAGGAGTCGATCAGCAGAACGTCAACGCCTGCGGCAACCAGCGCGTCaa > 1:2469107/1‑69 (MQ=255)
cacCTTCTGAGTGACCGTGGGAGGAGTCGATCAGCAGAACGTCAACGCCTGCGGCAACCAGCGCGTCaa > 1:1541330/1‑69 (MQ=255)
cacCTTCTGAGTGACCGTGGGAGGAGTCGATCAGCAGAACGTCAACGCCTGCGGCAACCAGCGCGTCaa > 1:2668009/1‑69 (MQ=255)
cacCTTCTGAGTGACCGTGGGAGGAGTCGATCAGCAGAACGTCAACGCCTGCGGCAACCAGCGCGTCaa > 1:2700329/1‑69 (MQ=255)
cacCTTCTGAGTGACCGTGGGAGGAGTCGATCAGCAGAACGTCAACGCCTGCGGCAACCAGCGCGTCaa > 1:2734812/1‑69 (MQ=255)
cacCTTCTGAGTGACCGTGGGAGGAGTCGATCAGCAGAACGTCAACGCCTGCGGCAACCAGCGCGTCaa > 1:1441178/1‑69 (MQ=255)
cacCTTCTGAGTGACCGTGGGAGGAGTCGATCAGCAGAACGTCAACGCCTGCGGCAACCAGCGCGTCa > 1:728669/1‑68 (MQ=255)
cacCTTCTGAGTGACCGTGGGAGGAGTCGATCAGCAGAACGTCAACGCCTGCGGCAACCAGCGCGTCa > 1:2819680/1‑68 (MQ=255)
|
AATTTGCAGATCCGGATATTTAGCACGGGTTTCACGGATACGTTGCAGTACACCTTCTGAGTGACCGTGGGAGGAGTCGATCAGCAGAACGTCAACGCCTGCGGCAACCAGCGCGTCAA > minE/1665938‑1666056
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
