Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,679,133 |
C→T |
39.7% |
G293G (GGG→GGA) |
pbpC ← |
fused transglycosylase and transpeptidase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,679,133 | 0 | C | T | 39.7%
| 35.4
/ 52.9
| 78 | G293G (GGG→GGA) | pbpC | fused transglycosylase and transpeptidase |
| Reads supporting (aligned to +/- strand): ref base C (8/39); new base T (15/16); total (23/55) |
| Fisher's exact test for biased strand distribution p-value = 4.83e-03 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.59e-01 |
ACGCATATCGGTATGATCAACCACGATCATCGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGACGTCGTTGAAGACCGGCATCC > minE/1679076‑1679184
|
acGCATATCGGTATGATCAACCACGATCATCGCCAGTGAGCTGCGCGGTGGCAATCGTCCTTTCCAGtt < 1:3015094/69‑1 (MQ=255)
acGCATATCGGTATGATCAACCACGATCATCGCCAGTGAGCTGCGCGGTGGCAATCGTCCTTTCCAGtt < 1:560807/69‑1 (MQ=255)
acGCATATCGGTATGATCAACCACGATCATCGCCAGTGAGCTGCGCGGTGGCAATCGTCCTTTCCAGtt < 1:529958/69‑1 (MQ=255)
acGCATATCGGTATGATCAACCACGATCATCGCCAGTGAGCTGCGCGGTGGCAATCGTCCTTTCCAGtt < 1:2042763/69‑1 (MQ=255)
acGCATATCGGTATGATCAACCACGATCATCGCCAGTGAGCTGCGCGGTGGCAATCGTCCTTTCCAGtt < 1:575061/69‑1 (MQ=255)
acGCATATCGGTATGATCAACCACGATCATCGCCAGTGAGCTGCGCGGTGGCAATCGTCCTTTCCAGtt < 1:1822558/69‑1 (MQ=255)
acGCATATCGGTATGATCAACCACGATCATCGCCAGTGAGCTGCGCGGTGGCAATCGTCCTTTCCAGtt < 1:1717184/69‑1 (MQ=255)
acGCATATCGGTATGATCAACCACGATCATCGCCAGTGAGCTGCGCGGTGGCAATCGTCCTTTCCAGtt < 1:616519/69‑1 (MQ=255)
acGCATATCGGTATGATCAACCACGATCATCGCCAGTGAGCTGCGCGGTGGCAATCGTCCTTTCCAGtt < 1:1645727/69‑1 (MQ=255)
acGCATATCGGTATGATCAACCACGATCATCGCCAGTGAGCTGCGCGGTGGCAATCGTCCTTTCCAGtt < 1:1536038/69‑1 (MQ=255)
acGCATATCGGTATGATCAACCACGATCATCGCCAGTGAGCTGCGCGGTGGCAATCGTCCTTTCCAGtt < 1:821813/69‑1 (MQ=255)
acGCATATCGGTATGATCAACCACGATCATCGCCAGTGAGCTGCGCGGTGGCAATCGTCCTTTCCAGtt < 1:27470/69‑1 (MQ=255)
acGCATATCGGTATGATCAACCACGATCATCGCCAGTGAGCTGCGCGGTGGCAATCGTCCTTTCCAGtt < 1:2960034/69‑1 (MQ=255)
acGCATATCGGTATGATCAACCACGATCATCGCCAGTGAGCTGCGCGGTGGCAATCGTCCTTTCCAGtt < 1:884073/69‑1 (MQ=255)
acGCATATCGGTATGATCAACCACGATCATCGCCAGTGAGCTGCGCGGTGGCAATCGTCCTTTCCAGtt < 1:1480869/69‑1 (MQ=255)
acGCATATCGGTATGATCAACCACGATCATCGCCAGTGAGCTGCGCGGTGGCAATCGTCCTTTCCAGtt < 1:858003/69‑1 (MQ=255)
tCGGTATGATCAACCACGATCATCGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGcc > 1:2117483/1‑69 (MQ=255)
aTCAACCACGATCATCGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGttct > 1:2459389/1‑67 (MQ=255)
aTCAACCACGATCATCGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGttct > 1:597508/1‑67 (MQ=255)
aTCAACCACGATCATCGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGttct > 1:2359707/1‑67 (MQ=255)
aCGATCATCGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGAcgt < 1:44806/69‑1 (MQ=255)
aCGATCATCGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGAcgt < 1:493832/69‑1 (MQ=255)
aCGATCATCGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGAcgt < 1:1431260/69‑1 (MQ=255)
aCGATCATCGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGAcgt < 1:2377504/69‑1 (MQ=255)
aCGATCATCGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGAcgt < 1:3254899/69‑1 (MQ=255)
aCGATCATCGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGAcgt < 1:2875580/69‑1 (MQ=255)
aCGATCATCGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGAcgt < 1:109695/69‑1 (MQ=255)
aCGATCATCGCCAGTGAGCTGCGCGGTGGCAACCGCACTTTCCAGTTTTGCGCCAGTTCTTCCAGAcgt < 1:2681446/69‑1 (MQ=255)
catcGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGAcgt < 1:2740007/64‑1 (MQ=255)
catcGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGACGTCGTTg < 1:220941/69‑1 (MQ=255)
catcGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGACGTCGTTg < 1:2041467/69‑1 (MQ=255)
catcGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGACGTCGTTg < 1:1049718/69‑1 (MQ=255)
catcGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGACGTCGTTg < 1:994524/69‑1 (MQ=255)
catcGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGACGTCGTTg < 1:1246398/69‑1 (MQ=255)
catcGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGACGTCGTTg < 1:1277543/69‑1 (MQ=255)
catcGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGACGTCGTTg < 1:744356/69‑1 (MQ=255)
atcGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGACGTCGTTg < 1:2119599/68‑1 (MQ=255)
atcGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGACGTCGTTg < 1:2192660/68‑1 (MQ=255)
cGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGACGTCGTTGAAg < 1:623025/69‑1 (MQ=255)
cGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGACGTCGTTGAAg < 1:2414910/69‑1 (MQ=255)
cGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGACGTCGTTGAAg < 1:1600445/69‑1 (MQ=255)
cGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGACGTCGTTGAAg < 1:389425/69‑1 (MQ=255)
cGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGACGTCGTTGAAg < 1:3354140/69‑1 (MQ=255)
cGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGACGTCGTTGAAg < 1:1597643/69‑1 (MQ=255)
cGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGACGTCGTTGAAg < 1:802687/69‑1 (MQ=255)
cGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGACGTCGTTGAAg < 1:1483979/69‑1 (MQ=255)
cGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGACGTCGTTGAAg < 1:3341254/69‑1 (MQ=255)
cGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGACGTCGTTGAAg < 1:583771/69‑1 (MQ=255)
cGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGACGTCGTTGAAg < 1:2833197/69‑1 (MQ=255)
cGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGACGTCGTTGAAg < 1:2273450/69‑1 (MQ=255)
gCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGACGTCGTTGAAg < 1:1067652/68‑1 (MQ=255)
tGAGCTGCGCGGTGGCAATCGTCCTTTCCAGTTTTGCGCCAGTTCTTCCAGAcgtc > 1:1519527/1‑56 (MQ=38)
tGAGCTGCGCGGTGGCAATCGTCCTTTCCAGTTTTGCGCCAGTTCTTCCAGACGTCGTTGAAGACCgg > 1:778881/1‑68 (MQ=255)
tGAGCTGCGCGGTGGCAATCGTCCTTTCCAGTTTTGCGCCAGTTCTTCCAGACGTCGTTGAAGACCGGc > 1:2252758/1‑69 (MQ=255)
tGAGCTGCGCGGTGGCAATCGTCCTTTCCAGTTTTGCGCCAGTTCTTCCAGACGTCGTTGAAGACCGGc > 1:1089744/1‑69 (MQ=255)
tGAGCTGCGCGGTGGCAATCGTCCTTTCCAGTTTTGCGCCAGTTCTTCCAGACGTCGTTGAAGACCGGc > 1:1491581/1‑69 (MQ=255)
tGAGCTGCGCGGTGGCAATCGTCCTTTCCAGTTTTGCGCCAGTTCTTCCAGACGTCGTTGAAGACCGGc > 1:1608054/1‑69 (MQ=255)
tGAGCTGCGCGGTGGCAATCGTCCTTTCCAGTTTTGCGCCAGTTCTTCCAGACGTCGTTGAAGACCGGc > 1:1704944/1‑69 (MQ=255)
tGAGCTGCGCGGTGGCAATCGTCCTTTCCAGTTTTGCGCCAGTTCTTCCAGACGTCGTTGAAGACCGGc > 1:1741820/1‑69 (MQ=255)
tGAGCTGCGCGGTGGCAATCGTCCTTTCCAGTTTTGCGCCAGTTCTTCCAGACGTCGTTGAAGACCGGc > 1:491290/1‑69 (MQ=255)
tGAGCTGCGCGGTGGCAATCGTCCTTTCCAGTTTTGCGCCAGTTCTTCCAGACGTCGTTGAAGACCGGc > 1:2603456/1‑69 (MQ=255)
tGAGCTGCGCGGTGGCAATCGTCCTTTCCAGTTTTGCGCCAGTTCTTCCAGACGTCGTTGAAGACCGGc > 1:393056/1‑69 (MQ=255)
tGAGCTGCGCGGTGGCAATCGTCCTTTCCAGTTTTGCGCCAGTTCTTCCAGACGTCGTTGAAGACCGGc > 1:2785853/1‑69 (MQ=255)
tGAGCTGCGCGGTGGCAATCGTCCTTTCCAGTTTTGCGCCAGTTCTTCCAGACGTCGTTGAAGACCGGc > 1:2665815/1‑69 (MQ=255)
tGAGCTGCGCGGTGGCAATCGTCCTTTCCAGTTTTGCGCCAGTTCTTCCAGACGTCGTTGAAGACCGGc > 1:3407129/1‑69 (MQ=255)
tGAGCTGCGCGGTGGCAATCGTCCTTTCCAGTTTTGCGCCAGTTCTTCCAGACGTCGTTGAAGACCGGc > 1:3407133/1‑69 (MQ=255)
gAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGACGTCGTTGAAGACCGGCa > 1:161942/1‑69 (MQ=255)
cTGCGCGGTGGCAATCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGACGTCGTTGAAGACCGGCATcc < 1:1688747/69‑1 (MQ=255)
cTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGACGTCGTTGAAGACCGGCATcc < 1:3228117/69‑1 (MQ=255)
cTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGACGTCGTTGAAGACCGGCATcc < 1:1356539/69‑1 (MQ=255)
cTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGACGTCGTTGAAGACCGGCATcc < 1:260397/69‑1 (MQ=255)
cTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGACGTCGTTGAAGACCGGCATcc < 1:203053/69‑1 (MQ=255)
cTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGACGTCGTTGAAGACCGGCATcc < 1:2200783/69‑1 (MQ=255)
cTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGACGTCGTTGAAGACCGGCATcc < 1:475440/69‑1 (MQ=255)
ggCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGACGTCGTTGAAGACCGGCATcc < 1:2505453/60‑1 (MQ=255)
gCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCa > 1:2764068/1‑36 (MQ=255)
gCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCa > 1:3097974/1‑36 (MQ=255)
gCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCa > 1:1483406/1‑36 (MQ=255)
|
ACGCATATCGGTATGATCAACCACGATCATCGCCAGTGAGCTGCGCGGTGGCAACCGCCCTTTCCAGTTTTGCGCCAGTTCTTCCAGACGTCGTTGAAGACCGGCATCC > minE/1679076‑1679184
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
