Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,688,153 |
T→G |
30.9% |
M298L (ATG→CTG) |
pepB ← |
aminopeptidase B |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,688,153 | 0 | T | G | 30.9%
| 76.1
/ 55.7
| 81 | M298L (ATG→CTG) | pepB | aminopeptidase B |
| Reads supporting (aligned to +/- strand): ref base T (25/31); new base G (8/17); total (33/48) |
| Fisher's exact test for biased strand distribution p-value = 3.34e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.56e-01 |
CGTGATAATCATTACCCAGCGCAGTTTTCGCCGCCCCGGTGAGGGTCGCCGCATCAATGATCATTTCCGGTTTCTGCGCACTGGCATCAATCAGACCATCGGCAAGCACCAGACGCCCTTCGGCATCAGTG > minE/1688090‑1688220
|
cgTGATAATCATTACCCAGCGCAGTTTTCGCCTCCCCGGTGAGGGTCGCCGCATCAATGATCATTTCCg > 1:2092537/1‑69 (MQ=255)
cgTGATAATCATTACCCAGCGCAGTTTTCGCCGCCCCGGTGATGGTCGCCGCATCAATGATCATTTCCg > 1:3132370/1‑69 (MQ=255)
cgTGATAATCATTACCCAGCGCAGTTTTCGCCGCCCCGGTGAGGGTCGCCGCATCAATGATCATTTCCg > 1:71754/1‑69 (MQ=255)
cgTGATAATCATTACCCAGCGCAGTTTTCGCCGCCCCGGTGAGGGTCGCCGCATCAATGATCATTTCCg > 1:2077912/1‑69 (MQ=255)
cgTGATAATCATTACCCAGCGCAGTTTTCGCCGCCCCGGTGAGGGTCGCCGCATCAATGATCATTTCCg > 1:344698/1‑69 (MQ=255)
cgTGATAATCATTACCCAGCGCAGTTTTCGCCGCCCCGGTGAGGGTCGCCGCATCAATGATCATTTCCg > 1:2602366/1‑69 (MQ=255)
aTCATTACCCAGCGCAGTTTTCGCCGCCCCGGTGAGGGTCGCCGCATCAATGATCATTTCCGGTTTCt > 1:1407237/1‑68 (MQ=255)
aTCATTACCCAGCGCAGTTTTCGCCGCCCCGGTGAGGGTCGCCGCATCAATGATCATTTCCGGTTTCTg > 1:478777/1‑69 (MQ=255)
aTCATTACCCAGCGCAGTTTTCGCCGCCCCGGTGAGGGTCGCCGCATCAATGATCATTTCCGGTTTCTg > 1:438499/1‑69 (MQ=255)
aTCATTACCCAGCGCAGTTTTCGCCGCCCCGGTGAGGGTCGCCGCATCAATGATCATTTCCGGTTTCTg > 1:401540/1‑69 (MQ=255)
aTCATTACCCAGCGCAGTTTTCGCCGCCCCGGTGAGGGTCGCCGCATCAATGATCATTTCCGGTTTCTg > 1:2528272/1‑69 (MQ=255)
aTTACCCAGCGCAGTTTTCGCCGCCCCGGTGAGGGTCGCCGCATCAATGATCATTTCCGGTTTCTgcgc > 1:1885612/1‑69 (MQ=255)
aTTACCCAGCGCAGTTTTCGCCGCCCCGGTGAGGGTCGCCGCATCAATGATCATTTCCGGTTTCTgcgc > 1:201613/1‑69 (MQ=255)
aTTACCCAGCGCAGTTTTCGCCGCCCCGGTGAGGGTCGCCGCATCAATGATCATTTCCGGTTTCTgcgc > 1:1733454/1‑69 (MQ=255)
aTTACCCAGCGCAGTTTTCGCCGCCCCGGTGAGGGTCGCCGCATCAATGATCATTTCCGGTTTCTgcgc > 1:3107586/1‑69 (MQ=255)
aTTACCCAGCGCAGTTTTCGCCGCCCCGGTGAGGGTCGCCGCATCAATGATCATTTCCGGTTTCTgcgc > 1:3365189/1‑69 (MQ=255)
aTTACCCAGCGCAGTTTTCGCCGCCCCGGTGAGGGTCGCCGCATCAATGATCATTTCCGGTTTCTgcgc > 1:135726/1‑69 (MQ=255)
aTTACCCAGCGCAGTTTTCGCCGCCCCGGTGAGGGTCGCCGCATCAATGATCATTTCCGGTTTCTgcgc > 1:1329720/1‑69 (MQ=255)
aTTACCCAGCGCAGTTTTCGCCGCCCCGGTGAGGGTCGCCGCATCAATGATCATTTCCGGTTTCTgcgc > 1:640312/1‑69 (MQ=255)
ttCGCCGCCCCGGTGAGGGTCGCCGCATCAATGATCATTTCCGGTTTCTgcgc < 1:2879381/53‑1 (MQ=255)
ttCGCCGCCCCGGTGAGGGTCGCCGCATCAATGATCATTTCCGGTTTCTgcgc < 1:3216723/53‑1 (MQ=255)
ttCGCCGCCCCGGTGAGGGTCGCCGCATCAATGATCATTTCCGGTTTCTgcgc < 1:2106989/53‑1 (MQ=255)
ttCGCCGCCCCGGTGAGGGTCGCCGCATCAATGATCATTTCCGGTTTCTgcgc < 1:969179/53‑1 (MQ=255)
ttCGCCGCCCCGGTGAGGGTCGCCGCATCAATGATCATTTCCGGTTTCTgcgc < 1:1872167/53‑1 (MQ=255)
ttCGCCGCCCCGGTGAGGGTCGCCGCATCAATGATCATTTCCGGTTTCTgcgc < 1:1683164/53‑1 (MQ=255)
ttCGCCGCCCCGGTGAGGGTCGCCGCATCAATGATCATTTCCGGTTTCTgcgc < 1:1076503/53‑1 (MQ=255)
ttCGCCGCCCCGGTGAGGGTCGCCGCATCAATGATCAGTTCCGGTTTCTGCGCACTGGCATCAATCAg > 1:190517/1‑68 (MQ=255)
ttCGCCGCCCCGGTGAGGGTCGCCGCATCAATGATCAGTTCCGGTTTCTGCGCACTGGCATCAATCAg > 1:2684328/1‑68 (MQ=255)
ttCGCCGCCCCGGTGAGGGTCGCCGCATCAATGATCAGTTCCGGTTTCTGCGCACTGGCATCAATCAg > 1:2655755/1‑68 (MQ=255)
ttCGCCGCCCCGGTGAGGGTCGCCGCATCAATGATCAGTTCCGGTTTCTGCGCACTGGCATCAATCAg > 1:3143179/1‑68 (MQ=255)
ttCGCCGCCCCGGTGAGGGTCGCCGCATCAATGATCAGTTCCGGTTTCTGCGCACTGGCATCAATCAg > 1:3227422/1‑68 (MQ=255)
ttCGCCGCCCCGGTGAGGGTCGCCGCATCAATGATCAGTTCCGGTTTCTGCGCACTGGCATCAATCAg > 1:1335343/1‑68 (MQ=255)
ttCGCCGCCCCGGTGAGGGTCGCCGCATCAATGATCAGTTCCGGTTTCTGCGCACTGGCATCAATCAg > 1:1228893/1‑68 (MQ=255)
ttCGCCGCCCCGGTGAGGGTCGCCGCATCAATGATCAGTTCCGGTTTCTGCGCACTGGCATCAATCAg > 1:1989502/1‑68 (MQ=255)
gccgccCCGGTGAGGGTCGCCGCATCAATGATCATTTCCGGTTTCTGCGCACTGGCATCAATCAGAcc < 1:1367479/68‑1 (MQ=255)
gccgccCCGGTGAGGGTCGCCGCATCAATGATCATTTCCGGTTTCTGCGCACTGGCATCAATCAGAcc < 1:1495517/68‑1 (MQ=255)
gccgccCCGGTGAGGGTCGCCGCATCAATGATCATTTCCGGTTTCTGCGCACTGGCATCAATCAGAcc < 1:3372554/68‑1 (MQ=255)
gccgccCCGGTGAGGGTCGCCGCATCAATGATCATTTCCGGTTTCTGCGCACTGGCATCAATCAGAcc < 1:157935/68‑1 (MQ=255)
gccgccCCGGTGAGGGTCGCCGCATCAATGATCATTTCCGGTTTCTGCGCACTGGCATCAATCAGAcc < 1:887455/68‑1 (MQ=255)
gccgccCCGGTGAGGGTCGCCGCATCAATGATCATTTCCGGTTTCTGCGCACTGGCATCAATCAGAcc < 1:2930635/68‑1 (MQ=255)
gccgccCCGGTGAGGGTCGCCGCATCAATGATCATTTCCGGTTTCTGCGCACTGGCATCAATCAGAcc < 1:1680002/68‑1 (MQ=255)
gccgccCCGGTGAGGGTCGCCGCATCAATGATCATTTCCGGTTTCTGCGCACTGGCATCAATCAGAcc < 1:1901186/68‑1 (MQ=255)
gccgccCCGGTGAGGGTCGCCGCATCAATGATCATTTCCGGTTTCTGCGCACTGGCATCAATCAGAcc < 1:2863614/68‑1 (MQ=255)
gccgccCCGGTGAGGGTCGCCGCATCAATGATCATTTCCGGTTTCTGCGCACTGGCATCAATCAGAcc < 1:107009/68‑1 (MQ=255)
gccgccCCGGTGAGGGTCGCCGCATCAATGATCATTTCCGGTTTCTGCGCACTGGCATCAATCAGAcc < 1:1735322/68‑1 (MQ=255)
cccGGTGAGGGTCGCCGCATCAATGATCAGTTCCGGTTTCTGCGCACTGGCATCAATCAGGCCATCGGc < 1:2499069/69‑1 (MQ=255)
cccGGTGAGGGTCGCCGCATCAATGATCAGTTCCGGTTTCTGCGCACTGGCATCAATCAGGCCATCGGc < 1:2023979/69‑1 (MQ=255)
cccGGTGAGGGTCGCCGCATCAATGATCAGTTCCGGTTTCTGCGCACTGGCATCAATCAGGCCATCGGc < 1:1747928/69‑1 (MQ=255)
cccGGTGAGGGTCGCCGCATCAATGATCAGTTCCGGTTTCTGCGCACTGGCATCAATCAGGCCATCGGc < 1:747689/69‑1 (MQ=255)
cccGGTGAGGGTCGCCGCATCAATGATCAGTTCCGGTTTCTGCGCACTGGCATCAATCAGGCCATCGGc < 1:707025/69‑1 (MQ=255)
cccGGTGAGGGTCGCCGCATCAATGATCAGTTCCGGTTTCTGCGCACTGGCATCAATCAGGCCATCGGc < 1:535297/69‑1 (MQ=255)
cccGGTGAGGGTCGCCGCATCAATGATCAGTTCCGGTTTCTGCGCACTGGCATCAATCAGGCCATCGGc < 1:419710/69‑1 (MQ=255)
cccGGTGAGGGTCGCCGCATCAATGATCAGTTCCGGTTTCTGCGCACTGGCATCAATCAGGCCATCGGc < 1:2483353/69‑1 (MQ=255)
cccGGTGAGGGTCGCCGCATCAATGATCAGTTCCGGTTTCTGCGCACTGGCATCAATCAGGCCATCGGc < 1:2732250/69‑1 (MQ=255)
cccGGTGAGGGTCGCCGCATCAATGATCAGTTCCGGTTTCTGCGCACTGGCATCAATCAGGCCATCGGc < 1:17164/69‑1 (MQ=255)
cccGGTGAGGGTCGCCGCATCAATGATCAGTTCCGGTTTCTGCGCACTGGCATCAATCAGGCCATCGGc < 1:1608533/69‑1 (MQ=255)
cccGGTGAGGGTCGCCGCATCAATGATCAGTTCCGGTTTCTGCGCACTGGCATCAATCAGGCCATCGGc < 1:3001629/69‑1 (MQ=255)
cccGGTGAGGGTCGCCGCATCAATGATCAGTTCCGGTTTCTGCGCACTGGCATCAATCAGGCCATCGGc < 1:2998109/69‑1 (MQ=255)
cccGGTGAGGGTCGCCGCATCAATGATCAGTTCCGGTTTCTGCGCACTGGCATCAATCAGGCCATCGGc < 1:2563556/69‑1 (MQ=255)
cccGGTGAGGGTCGCCGCATCAATGATCAGTTCCGGTTTCTGCGCACTGGCATCAATCAGGCCATCGGc < 1:306553/69‑1 (MQ=255)
ccGGTGAGGGTCGCCGCATCAATGATCAGTTCCGGTTTCTGCGCACTGGCATCAATCAGGCCATCGGc < 1:1606754/68‑1 (MQ=255)
gAGGGTCGCCGCATCAATGATCATTTCCGGTTTCTGCGCACTGGCATCAATCAGACCATTGGCAAg > 1:3324862/1‑66 (MQ=255)
gAGGGTCGCCGCATCAATGATCATTTCCGGTTTCTGCGCACTGGCATCAATCAGACCATTGGCAAGc > 1:1657347/1‑67 (MQ=255)
gAGGGTCGCCGCATCAATGATCATTTCCGGTTTCTGCGCACTGGCATCAATCAGACCATTGGCAAGc > 1:2778587/1‑67 (MQ=255)
gAGGGTCGCCGCATCAATGATCATTTCCGGTTTCTGCGCACTGGCATCAATCAGACCATTGGCAAGc > 1:786228/1‑67 (MQ=255)
gAGGGTCGCCGCATCAATGATCATTTCCGGTTTCTGCGCACTGGCATCAATCAGACCATTGGCAAGc > 1:2827475/1‑67 (MQ=255)
gAGGGTCGCCGCATCAATGATCATTTCCGGTTTCTGCGCACTGGCATCAATCAGACCATTGGCAAGc > 1:2303384/1‑67 (MQ=255)
gccgcATCATTGATCATTTCCGGTTTCTGCGCACTGGCATCAATCAGACCATCGGCAAGCACCAGACGc < 1:2667466/69‑1 (MQ=255)
gccgcATCAATGATCATTTCCGGTTTCTGTGCACTGGCATCAATCAGACCATCGGCAAGCACCAGACGc < 1:1569081/69‑1 (MQ=255)
gccgcATCAATGATCATTTCCGGTTTCTGCGCACTGGCATCAATCAGACCATCGGCAAGCACCAGAccc < 1:1733233/69‑3 (MQ=255)
gccgcATCAATGATCATTTCCGGTTTCTGCGCACTGGCATCAATCAGACCATCGGCAAGCACCAGACGc < 1:2974890/69‑1 (MQ=255)
gccgcATCAATGATCATTTCCGGTTTCTGCGCACTGGCATCAATCAGACCATCGGCAAGCACCAGACGc < 1:2998082/69‑1 (MQ=255)
gccgcATCAATGATCATTTCCGGTTTCTGCGCACTGGCATCAATCAGACCATCGGCAAGCACCAGACGc < 1:2555895/69‑1 (MQ=255)
gccgcATCAATGATCATTTCCGGTTTCTGCGCACTGGCATCAATCAGACCATCGGCAAGCACCAGACGc < 1:2485602/69‑1 (MQ=255)
gccgcATCAATGATCATTTCCGGTTTCTGCGCACTGGCATCAATCAGACCATCGGCAAGCACCAGACGc < 1:3262508/69‑1 (MQ=255)
gccgcATCAATGATCATTTCCGGTTTCTGCGCACTGGCATCAATCAGACCATCGGCAAGCACCAGACGc < 1:3017788/69‑1 (MQ=255)
gccgcATCAATGATCATTTCCGGTTTCTGCGCACTGGCATCAATCAGACCATCGGCAAGCACCAGACGc < 1:2733365/69‑1 (MQ=255)
ccgcATCAATGATCAGTTCCGGTTTCTGCGCACTGGCATCAATCAGGCCATCGGc < 1:217616/55‑1 (MQ=39)
cATCAATGATCATTTCCGGTTTCTGCGCACTGGCATCAATCGGAcc < 1:2357818/46‑1 (MQ=255)
aTTTCCGGTTTCTGCGCACTGGCATCAATCAGACCATCGGCAAGCACCAGACGCCCTTCGGCATCAgtg < 1:3202858/69‑1 (MQ=255)
aTTTCCGGTTTCTGCGCACTGGCATCAATCAGACCATCGGCAAGCACCAGACGCCATTCGGCATCAgtg < 1:3299634/69‑1 (MQ=255)
|
CGTGATAATCATTACCCAGCGCAGTTTTCGCCGCCCCGGTGAGGGTCGCCGCATCAATGATCATTTCCGGTTTCTGCGCACTGGCATCAATCAGACCATCGGCAAGCACCAGACGCCCTTCGGCATCAGTG > minE/1688090‑1688220
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
