Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,688,958 |
G→A |
47.6% |
D29D (GAC→GAT) |
pepB ← |
aminopeptidase B |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,688,958 | 0 | G | A | 47.6%
| 3.7
/ 52.5
| 63 | D29D (GAC→GAT) | pepB | aminopeptidase B |
| Reads supporting (aligned to +/- strand): ref base G (18/15); new base A (6/24); total (24/39) |
| Fisher's exact test for biased strand distribution p-value = 8.75e-03 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.01e-01 |
GCGCCGCACGCTGGATCAGCCCCAGATCGTCTGCCCCGTTCAAATGCAGGGTAATGCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTA > minE/1688900‑1689024
|
gcgcCGCACGCTGGATCAACCCCAGATCGTCTGCCCCGTTCAAATGCAGGGTAATGCCATCatta < 1:2428300/65‑1 (MQ=255)
gcgcCGCACGCTGGATCAACCCCAGATCGTCTGCCCCGTTCAAATGCAGGGTAATGCCATCatta < 1:1097160/65‑1 (MQ=255)
gcgcCGCACGCTGGATCAACCCCAGATCGTCTGCCCCGTTCAAATGCAGGGTAATGCCATCatta < 1:1147551/65‑1 (MQ=255)
gcgcCGCACGCTGGATCAACCCCAGATCGTCTGCCCCGTTCAAATGCAGGGTAATGCCATCatta < 1:2491666/65‑1 (MQ=255)
gcgcCGCACGCTGGATCAACCCCAGATCGTCTGCCCCGTTCAAATGCAGGGTAATGCCATCatta < 1:1319057/65‑1 (MQ=255)
gcgcCGCACGCTGGATCAACCCCAGATCGTCTGCCCCGTTCAAATGCAGGGTAATGCCATCatta < 1:50047/65‑1 (MQ=255)
gcgcCGCACGCTGGATCAACCCCAGATCGTCTGCCCCGTTCAAATGCAGGGTAATGCCATCatta < 1:3384049/65‑1 (MQ=255)
gcgcCGCACGCTGGATCAACCCCAGATCGTCTGCCCCGTTCAAATGCAGGGTAATGCCATCatta < 1:2693846/65‑1 (MQ=255)
gcgcCGCACGCTGGATCAACCCCAGATCGTCTGCCCCGTTCAAATGCAGGGTAATGCCATCatta < 1:1519399/65‑1 (MQ=255)
gcgcCGCACGCTGGATCAACCCCAGATCGTCTGCCCCGTTCAAATGCAGGGTAATGCCATCatta < 1:2975988/65‑1 (MQ=255)
gcgcCGCACGCTGGATCAACCCCAGATCGTCTGCCCCGTTCAAATGCAGGGTAATGCCATCatta < 1:3004207/65‑1 (MQ=255)
gcgcCGCACGCTGGATCAACCCCAGATCGTCTGCCCCGTGCAAATGCAGTGTAATGCCATCatta < 1:1092794/65‑1 (MQ=255)
cgccgcACGCTGGATCAACCCCAGATCGTCTGCCCCGTTCAAATGCAGGGTAATGCCATCatta < 1:919042/64‑1 (MQ=255)
ccGTTCAAATGCAGTGTAATGCCATCATTATTAATGCTGTAAGTCGCTTTTTCTCCCCAACGTGCGTc < 1:3381662/68‑1 (MQ=255)
ccGTTCAAATGCAGGGTAATGCCATCATTATTAATGCTGTAAGTCGCTTTTTCTCCCCAACGTGCGTc < 1:3067695/68‑1 (MQ=255)
ccGTTCAAATGCAGGGTAATGCCATCATTATTAATGCTGTAAGTCGCTTTTTCTCCCCAACGTGCGTc < 1:3138779/68‑1 (MQ=255)
ccGTTCAAATGCAGGGTAATGCCATCATTATTAATGCTGTAAGTCGCTTTTTCTCCCCAACGTGCGTc < 1:3198250/68‑1 (MQ=255)
ccGTTCAAATGCAGGGTAATGCCATCATTATTAATGCTGTAAGTCGCTTTTTCTCCCCAACGTGCGTc < 1:648952/68‑1 (MQ=255)
ccGTTCAAATGCAGGGTAATGCCATCATTATTAATGCTGTAAGTCGCTTTTTCTCCCCAACGTGCGTc < 1:1041602/68‑1 (MQ=255)
ccGTTCAAATGCAGGGTAATGCCATCATTATTAATGCTGTAAGTCGCTTTTTCTCCCCAACGTGCGTc < 1:2236463/68‑1 (MQ=255)
ccGTTCAAATGCAGGGTAATGCCATCATTATTAATGCTGTAAGTCGCTTTTTCTCCCCAACGTGCGTc < 1:2156153/68‑1 (MQ=255)
ccGTTCAAATGCAGGGTAATGCCATCATTATTAATGCTGTAAGTCGCTTTTTCTCCCCAACGTGCGTc < 1:1540226/68‑1 (MQ=255)
ccGTTCAAATGCAGGGTAATGCCATCATTATTAATGCTGTAAGTCGCTTTTTCTCCCCAACGTGCGTc < 1:1471215/68‑1 (MQ=255)
ccGTTCAAATGCAGGGTAATGCCATCATTATTAATGCTGTAAGTCGCTTTTTCTCCCCAACGTGCGTc < 1:1392527/68‑1 (MQ=255)
gTTCAAATGCAGGGTAATGCCATCATTATTAATGCTGTAAGTCGCt > 1:2389301/1‑46 (MQ=255)
gTTCAAATGCAGGGTAATGCCATCATTATTAATGCTGTAAGTCGCTTTTTCTCCCCAACGTGCGTCGGc > 1:1627588/1‑69 (MQ=255)
gTTCAAATGCAGGGTAATGCCATCATTATTAATGCTGTAAGTCGCTTTTTCTCCCCAACGTGCGTCGGc > 1:2230820/1‑69 (MQ=255)
gTTCAAATGCAGGGTAATGCCATCATTATTAATGCTGTAAGTCGCTTTTTCTCCCCAACGTGCGTCGGc > 1:2061063/1‑69 (MQ=255)
gTTCAAATGCAGGGTAATGCCATCATTATTAATGCTGTAAGTCGCTTTTTCTCCCCAACGTGCGTCGGc > 1:1304247/1‑69 (MQ=255)
gTTCAAATGCAGGGTAATGCCATCATTATTAATGCTGTAAGTCGCTTTTTCTCCCCAACGTGCGTCGGc > 1:378631/1‑69 (MQ=255)
cAAATGCAGGGTAATGCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAgg > 1:1893135/1‑69 (MQ=255)
cAAATGCAGGGTAATGCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAgg > 1:1321602/1‑69 (MQ=255)
aaTGCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTagagag < 1:3056761/69‑1 (MQ=255)
aaTGCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTagagag < 1:331068/69‑1 (MQ=255)
aaTGCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTagagag < 1:3415629/69‑1 (MQ=255)
aaTGCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTagagag < 1:1847801/69‑1 (MQ=255)
aaTGCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTagagag < 1:246518/69‑1 (MQ=255)
aTGCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCa > 1:73599/1‑54 (MQ=255)
aTGCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCa > 1:320665/1‑54 (MQ=255)
aTGCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCa > 1:741060/1‑54 (MQ=255)
aTGCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCa > 1:3183074/1‑54 (MQ=255)
aTGCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCa > 1:1017206/1‑54 (MQ=255)
aTGCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCa > 1:1654394/1‑54 (MQ=255)
aTGCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCa > 1:270042/1‑54 (MQ=255)
aTGCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCa > 1:2175684/1‑54 (MQ=255)
aTGCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCa > 1:209667/1‑54 (MQ=255)
aTGCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCa > 1:2441184/1‑54 (MQ=255)
aTGCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCa > 1:2656525/1‑54 (MQ=255)
aTGCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCa > 1:2103341/1‑54 (MQ=255)
aTGCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCa > 1:2805305/1‑54 (MQ=255)
aTGCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCa > 1:2950381/1‑54 (MQ=255)
gCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAg < 1:62848/53‑1 (MQ=255)
gCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGt < 1:2822012/69‑1 (MQ=255)
gCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGt < 1:710337/69‑1 (MQ=255)
gCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGt < 1:1630082/69‑1 (MQ=255)
ccGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTagagag < 1:2909458/65‑1 (MQ=255)
ccGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTagagag < 1:3200204/65‑1 (MQ=255)
ccGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTagagag < 1:2946993/65‑1 (MQ=255)
ccGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTagagag < 1:679838/65‑1 (MQ=255)
ccGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTagagag < 1:2248216/65‑1 (MQ=255)
ccGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTagagag < 1:1692665/65‑1 (MQ=255)
ccGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTa > 1:2860118/1‑69 (MQ=255)
ccGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTa > 1:259869/1‑69 (MQ=255)
|
GCGCCGCACGCTGGATCAGCCCCAGATCGTCTGCCCCGTTCAAATGCAGGGTAATGCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTA > minE/1688900‑1689024
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
