Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F1 I0 R1	4577	57.4	3436498	77.7%	2670158	63.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	1,688,994	G→A	20.4%	R17R (CGC→CGT)	pepB ←	aminopeptidase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,688,994	0	G	A	20.4%	113.4 / 19.5	78	R17R (CGC→CGT)	pepB	aminopeptidase B
Reads supporting (aligned to +/- strand): ref base G (26/36); new base A (5/11); total (31/47)
Fisher's exact test for biased strand distribution p-value = 5.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.48e-01

CCGTTCAAATGCAGGGTAATGCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTC  >  minE/1688935‑1689058

ccGTTCAAATGCAGTGTAATGCCATCATTATTAATGCTGTAAGTCGCTTTTTCTCCCCAACGTGCGTc                                                          <  1:3381662/68‑1 (MQ=255)

ccGTTCAAATGCAGGGTAATGCCATCATTATTAATGCTGTAAGTCGCTTTTTCTCCCCAACGTGCGTc                                                          <  1:1041602/68‑1 (MQ=255)

ccGTTCAAATGCAGGGTAATGCCATCATTATTAATGCTGTAAGTCGCTTTTTCTCCCCAACGTGCGTc                                                          <  1:2236463/68‑1 (MQ=255)

ccGTTCAAATGCAGGGTAATGCCATCATTATTAATGCTGTAAGTCGCTTTTTCTCCCCAACGTGCGTc                                                          <  1:2156153/68‑1 (MQ=255)

ccGTTCAAATGCAGGGTAATGCCATCATTATTAATGCTGTAAGTCGCTTTTTCTCCCCAACGTGCGTc                                                          <  1:3067695/68‑1 (MQ=255)

ccGTTCAAATGCAGGGTAATGCCATCATTATTAATGCTGTAAGTCGCTTTTTCTCCCCAACGTGCGTc                                                          <  1:3138779/68‑1 (MQ=255)

ccGTTCAAATGCAGGGTAATGCCATCATTATTAATGCTGTAAGTCGCTTTTTCTCCCCAACGTGCGTc                                                          <  1:3198250/68‑1 (MQ=255)

ccGTTCAAATGCAGGGTAATGCCATCATTATTAATGCTGTAAGTCGCTTTTTCTCCCCAACGTGCGTc                                                          <  1:1540226/68‑1 (MQ=255)

ccGTTCAAATGCAGGGTAATGCCATCATTATTAATGCTGTAAGTCGCTTTTTCTCCCCAACGTGCGTc                                                          <  1:1471215/68‑1 (MQ=255)

ccGTTCAAATGCAGGGTAATGCCATCATTATTAATGCTGTAAGTCGCTTTTTCTCCCCAACGTGCGTc                                                          <  1:648952/68‑1 (MQ=255)

ccGTTCAAATGCAGGGTAATGCCATCATTATTAATGCTGTAAGTCGCTTTTTCTCCCCAACGTGCGTc                                                          <  1:1392527/68‑1 (MQ=255)

  gTTCAAATGCAGGGTAATGCCATCATTATTAATGCTGTAAGTCGCTTTTTCTCCCCAACGTGCGTCGGc                                                       >  1:378631/1‑69 (MQ=255)

  gTTCAAATGCAGGGTAATGCCATCATTATTAATGCTGTAAGTCGCTTTTTCTCCCCAACGTGCGTCGGc                                                       >  1:1627588/1‑69 (MQ=255)

  gTTCAAATGCAGGGTAATGCCATCATTATTAATGCTGTAAGTCGCTTTTTCTCCCCAACGTGCGTCGGc                                                       >  1:2230820/1‑69 (MQ=255)

  gTTCAAATGCAGGGTAATGCCATCATTATTAATGCTGTAAGTCGCTTTTTCTCCCCAACGTGCGTCGGc                                                       >  1:2061063/1‑69 (MQ=255)

  gTTCAAATGCAGGGTAATGCCATCATTATTAATGCTGTAAGTCGCTTTTTCTCCCCAACGTGCGTCGGc                                                       >  1:1304247/1‑69 (MQ=255)

     cAAATGCAGGGTAATGCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAgg                                                    >  1:1321602/1‑69 (MQ=255)

     cAAATGCAGGGTAATGCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAgg                                                    >  1:1893135/1‑69 (MQ=255)

                 aaTGCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTagagag                                        <  1:246518/69‑1 (MQ=255)

                 aaTGCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTagagag                                        <  1:3415629/69‑1 (MQ=255)

                 aaTGCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTagagag                                        <  1:3056761/69‑1 (MQ=255)

                 aaTGCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTagagag                                        <  1:1847801/69‑1 (MQ=255)

                 aaTGCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTagagag                                        <  1:331068/69‑1 (MQ=255)

                  aTGCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCa                                                      >  1:3183074/1‑54 (MQ=255)

                  aTGCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCa                                                      >  1:73599/1‑54 (MQ=255)

                  aTGCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCa                                                      >  1:2950381/1‑54 (MQ=255)

                  aTGCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCa                                                      >  1:2805305/1‑54 (MQ=255)

                  aTGCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCa                                                      >  1:270042/1‑54 (MQ=255)

                  aTGCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCa                                                      >  1:741060/1‑54 (MQ=255)

                  aTGCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCa                                                      >  1:1017206/1‑54 (MQ=255)

                  aTGCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCa                                                      >  1:320665/1‑54 (MQ=255)

                  aTGCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCa                                                      >  1:2656525/1‑54 (MQ=255)

                  aTGCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCa                                                      >  1:2441184/1‑54 (MQ=255)

                  aTGCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCa                                                      >  1:1654394/1‑54 (MQ=255)

                  aTGCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCa                                                      >  1:209667/1‑54 (MQ=255)

                  aTGCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCa                                                      >  1:2103341/1‑54 (MQ=255)

                  aTGCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCa                                                      >  1:2175684/1‑54 (MQ=255)

                    gCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAg                                                     <  1:62848/53‑1 (MQ=255)

                    gCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGt                                     <  1:710337/69‑1 (MQ=255)

                    gCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGt                                     <  1:1630082/69‑1 (MQ=255)

                    gCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGt                                     <  1:2822012/69‑1 (MQ=255)

                     ccGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTagagag                                        <  1:679838/65‑1 (MQ=255)

                     ccGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTagagag                                        <  1:2909458/65‑1 (MQ=255)

                     ccGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTagagag                                        <  1:2946993/65‑1 (MQ=255)

                     ccGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTagagag                                        <  1:3200204/65‑1 (MQ=255)

                     ccGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTagagag                                        <  1:2248216/65‑1 (MQ=255)

                     ccGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTagagag                                        <  1:1692665/65‑1 (MQ=255)

                     ccGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTa                                    >  1:259869/1‑69 (MQ=255)

                     ccGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTa                                    >  1:2860118/1‑69 (MQ=255)

                        tCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAAt                                  >  1:303032/1‑68 (MQ=255)

                         cATTATTAATGCTGTAAGTTGCTTTTTCTCCCCCGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATc                                 >  1:1453251/1‑68 (MQ=255)

                          attattAATGCTGTAAGTTGCTATTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTa                                    >  1:2642593/1‑64 (MQ=255)

                           ttattaATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTg                                                 <  1:666993/50‑1 (MQ=255)

                           ttattaATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTg                                                 <  1:1617780/50‑1 (MQ=255)

                            tattaATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTc                              <  1:3107580/68‑1 (MQ=255)

                            tattaATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTc                              <  1:920545/68‑1 (MQ=255)

                             attaATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCAt                            >  1:1445877/1‑69 (MQ=255)

                                  tGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCt                        <  1:59696/68‑1 (MQ=255)

                                  tGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCt                        <  1:939100/68‑1 (MQ=255)

                                  tGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCt                        <  1:2443790/68‑1 (MQ=255)

                                  tGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCt                        <  1:2568826/68‑1 (MQ=255)

                                  tGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCt                        <  1:2638328/68‑1 (MQ=255)

                                    cTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTc                      >  1:1735524/1‑68 (MQ=255)

                                    cTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTc                      >  1:1434499/1‑68 (MQ=255)

                                    cTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCt                     >  1:1363567/1‑69 (MQ=255)

                                    cTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCt                     >  1:3306493/1‑69 (MQ=255)

                                            tGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCAtttt             <  1:1407937/69‑1 (MQ=255)

                                            tGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCAtttt             <  1:1259078/69‑1 (MQ=255)

                                            tGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCAtttt             <  1:737156/69‑1 (MQ=255)

                                            tGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCAtttt             <  1:2801964/69‑1 (MQ=255)

                                            tGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCAtttt             <  1:1970577/69‑1 (MQ=255)

                                            tGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCAtttt             <  1:3435730/69‑1 (MQ=255)

                                            tGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCAtttt             <  1:1809201/69‑1 (MQ=255)

                                            tGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCAtttt             <  1:3167207/69‑1 (MQ=255)

                                             gCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTc                              <  1:2097572/51‑1 (MQ=255)

                                                         cAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCcttc  <  1:656897/67‑1 (MQ=255)

                                                         cAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCcttc  <  1:2882544/67‑1 (MQ=255)

                                                         cAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCcttc  <  1:626540/67‑1 (MQ=255)

CCGTTCAAATGCAGGGTAATGCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTC  >  minE/1688935‑1689058

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A