Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F1 I0 R1	4577	57.4	3436498	77.7%	2670158	63.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	1,781,726	C→T	23.3%	Y59Y (TAC→TAT)	yfjD →	predicted inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,781,726	0	C	T	23.3%	149.6 / 35.0	111	Y59Y (TAC→TAT)	yfjD	predicted inner membrane protein
Reads supporting (aligned to +/- strand): ref base C (52/33); new base T (18/8); total (70/41)
Fisher's exact test for biased strand distribution p-value = 4.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.13e-01

Only 100 of 115 total aligned reads displayed.

TTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGAGGTAT  >  minE/1781658‑1781793

ttAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTAc                                                                     >  1:2387500/1‑69 (MQ=255)

ttAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTAc                                                                     >  1:1378778/1‑69 (MQ=255)

    tCGGCAATAATCTGGTCAATATCCTTGCTTCCGCACTCGGCACTATTGTCGGGATGCGCTTGTATGGc                                                                  <  1:1895089/68‑1 (MQ=255)

    tCGGCAATAATCTGGTCAATATCCTTGCTTCCGCACTCGGCACTATTGTCGGGATGCGCTTGTATGGc                                                                  <  1:675052/68‑1 (MQ=255)

    tCGGCAATAATCTGGTCAATATCCTTGCTTCCGCACTCGGCACTATTGTCGGGATGCGCTTGTATGGc                                                                  <  1:194329/68‑1 (MQ=255)

    tCGGCAATAATCTGGTCAATATCCTTGCTTCCGCACTCGGCACTATTGTCGGGATGCGCTTGTATGGc                                                                  <  1:635200/68‑1 (MQ=255)

    tCGGCAATAATCTGGTCAATATCCTTGCTTCCGCACTCGGCACTATTGTCGGGATGCGCTTGTATGGc                                                                  <  1:2835426/68‑1 (MQ=255)

     cGGCAATAATCTGGTCAATATCCTTGCTTCCGCACTCGGCACTATTGTCGGGATGCGCTTGTATGGc                                                                  <  1:3029205/67‑1 (MQ=255)

      ggCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGAt                                                               >  1:2041821/1‑69 (MQ=255)

            aaCCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGc                                                         >  1:1583131/1‑69 (MQ=255)

             aCCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCggg                                                          <  1:1066511/67‑1 (MQ=255)

             aCCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCggg                                                          <  1:187804/67‑1 (MQ=255)

             aCCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCggg                                                          <  1:2233189/67‑1 (MQ=255)

             aCCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCggg                                                          <  1:2505300/67‑1 (MQ=255)

             aCCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCggg                                                          >  1:2692847/1‑67 (MQ=255)

             aCCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCggg                                                          <  1:2846702/67‑1 (MQ=255)

             aCCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCggg                                                          >  1:2963043/1‑67 (MQ=255)

             aCCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCggg                                                          <  1:3083514/67‑1 (MQ=255)

             aCCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCggg                                                          <  1:419064/67‑1 (MQ=255)

             aCCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCggg                                                          >  1:505960/1‑67 (MQ=255)

             aCCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCggg                                                          <  1:552721/67‑1 (MQ=255)

                 ggTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGc                                                    <  1:2593498/69‑1 (MQ=255)

                 ggTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGc                                                    <  1:2381755/69‑1 (MQ=255)

                    cAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAAt                                                 <  1:2935050/69‑1 (MQ=255)

                    cAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAAt                                                 <  1:1239142/69‑1 (MQ=255)

                    cAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAAt                                                 <  1:763862/69‑1 (MQ=255)

                      atatTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAAt                                                 <  1:2522312/67‑1 (MQ=255)

                       tatTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAAtt                                                >  1:2963841/1‑67 (MQ=255)

                       tatTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTg                                               >  1:2494517/1‑68 (MQ=255)

                        atTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCg                                                        >  1:2816003/1‑58 (MQ=255)

                        atTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGc                                              >  1:2820023/1‑68 (MQ=255)

                        atTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGc                                              >  1:2087856/1‑68 (MQ=255)

                        atTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGc                                              >  1:2399167/1‑68 (MQ=255)

                        atTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCg                                             >  1:187557/1‑69 (MQ=255)

                        atTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCg                                             >  1:2841937/1‑69 (MQ=255)

                        atTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCg                                             >  1:3341696/1‑69 (MQ=255)

                        atTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCg                                             >  1:477606/1‑69 (MQ=255)

                         ttCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGa                                            >  1:1280030/1‑69 (MQ=255)

                                     cgcTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCt                                  <  1:2904288/67‑1 (MQ=255)

                                     cgcTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCt                                  <  1:1527102/67‑1 (MQ=255)

                                     cgcTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCt                                  <  1:568684/67‑1 (MQ=255)

                                     cgcTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCt                                  <  1:556798/67‑1 (MQ=255)

                                         cGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACttt                            >  1:2964842/1‑69 (MQ=255)

                                                aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGc                                              >  1:1420443/1‑44 (MQ=255)

                                                aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCg                                             >  1:1897545/1‑45 (MQ=255)

                                                aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACt                                          >  1:1402707/1‑48 (MQ=255)

                                                aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGTCTTTTGTCGt                      >  1:3410559/1‑68 (MQ=255)

                                                aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTc                        >  1:3086236/1‑66 (MQ=255)

                                                aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGt                      >  1:2374221/1‑68 (MQ=255)

                                                aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGt                      >  1:773059/1‑68 (MQ=255)

                                                aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGt                      >  1:3237664/1‑68 (MQ=255)

                                                aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGt                      >  1:730417/1‑68 (MQ=255)

                                                aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGt                      >  1:1976946/1‑68 (MQ=255)

                                                aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGt                      >  1:2786024/1‑68 (MQ=255)

                                                aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGt                      >  1:653992/1‑68 (MQ=255)

                                                aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGt                      >  1:1560504/1‑68 (MQ=255)

                                                aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGt                      >  1:2055274/1‑68 (MQ=255)

                                                aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGt                      >  1:2098523/1‑68 (MQ=255)

                                                aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGt                      >  1:2371700/1‑68 (MQ=255)

                                                aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTa                     >  1:3359722/1‑69 (MQ=255)

                                                aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTa                     <  1:493486/69‑1 (MQ=255)

                                                aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTa                     >  1:887564/1‑69 (MQ=255)

                                                aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTa                     >  1:42187/1‑69 (MQ=255)

                                                aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTa                     <  1:489925/69‑1 (MQ=255)

                                                aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTa                     >  1:1347952/1‑69 (MQ=255)

                                                aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTa                     <  1:1895713/69‑1 (MQ=255)

                                                aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTa                     >  1:1386945/1‑69 (MQ=255)

                                                aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTa                     >  1:1921077/1‑69 (MQ=255)

                                                aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTa                     >  1:2134023/1‑69 (MQ=255)

                                                aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTa                     <  1:1051496/69‑1 (MQ=255)

                                                aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTa                     <  1:2319367/69‑1 (MQ=255)

                                                 attttgGGATGCGTTTGTACGGCGATGCGGGCGTGGCAAATGCGACTGGTGTGCTGACTTTTGTCGTAc                    >  1:1753910/4‑69 (MQ=255)

                                                         aTGCGCTTGTATGGCGATGCTGGCGTGGCAATTGCGACTGGCGTGCTGAcct                             >  1:952090/1‑50 (MQ=37)

                                                         aTGCGCTTGTATGGCGATGCTGGCGTGGCAATTGCGACTGGCGTGCTGAcct                             >  1:1350990/1‑50 (MQ=37)

                                                         aTGCGCTTGTATGGCGATGCTGGCGTGGCAATTGCGACTGGCGTGCTGAcct                             >  1:787040/1‑50 (MQ=37)

                                                         aTGCGCTTGTATGGCGATGCTGGCGTGGCAATTGCGACTGGCGTGCTGAcct                             >  1:1935664/1‑50 (MQ=37)

                                                         aTGCGCTTGTATGGCGATGCTGGCGTGGCAATTGCGACTGGCGTGCTGAcct                             >  1:1163817/1‑50 (MQ=37)

                                                         aTGCGCTTGTATGGCGATGCTGGCGTGGCAATTGCGACTGGCGTGCTGAcct                             >  1:117257/1‑50 (MQ=37)

                                                         aTGCGCTTGTATGGCGATGCTGGCGTGGCAATTGCGACTGGCGTGCTGAcct                             >  1:598451/1‑50 (MQ=37)

                                                         aTGCGCTTGTATGGCGATGCTGGCGTGGCAATTGCGACTGGCGTGCTGAcct                             >  1:1323499/1‑50 (MQ=37)

                                                         aTGCGCTTGTATGGCGATGCTGGCGTGGCAATTGCGACTGGCGTGCTGAcct                             >  1:170944/1‑50 (MQ=37)

                                                         aTGCGCTTGTATGGCGATGCTGGCGTGGCAATTGCGACTGGCGTGCTGAcct                             >  1:1326008/1‑50 (MQ=37)

                                                         aTGCGCTTGTATGGCGATGCTGGCGTGGCAATTGCGACTGGCGTGCTGAcct                             >  1:3061494/1‑50 (MQ=37)

                                                         aTGCGCTTGTATGGCGATGCTGGCGTGGCAATTGCGACTGGCGTGCTGAcct                             >  1:3139369/1‑50 (MQ=37)

                                                         aTGCGCTTGTATGGCGATGCTGGCGTGGCAATTGCGACTGGCGTGCTGAcct                             >  1:1469274/1‑50 (MQ=37)

                                                         aTGCGCTTGTATGGCGATGCTGGCGTGGCAATTGCGACTGGCGTGCTGAcct                             >  1:3356089/1‑50 (MQ=37)

                                                         aTGCGCTTGTATGGCGATGCTGGCGTGGCAATTGCGACTGGCGTGCTGAcct                             >  1:1575575/1‑50 (MQ=37)

                                                         aTGCGCTTGTATGGCGATGCTGGCGTGGCAATTGCGACTGGCGTGCTGAcct                             >  1:1644877/1‑50 (MQ=37)

                                                              tttGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGa       >  1:2217939/1‑69 (MQ=255)

                                                              tttGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGa       >  1:185610/1‑69 (MQ=255)

                                                              tttGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGa       >  1:3132694/1‑69 (MQ=255)

                                                                tGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGAgg     <  1:535957/69‑1 (MQ=255)

                                                                tGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGAgg     <  1:3331871/69‑1 (MQ=255)

                                                                tGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGAgg     <  1:1259206/69‑1 (MQ=255)

                                                                tGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGAgg     <  1:2016217/69‑1 (MQ=255)

                                                                tGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGAgg     <  1:1740080/69‑1 (MQ=255)

                                                                tGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGAgg     <  1:382211/69‑1 (MQ=255)

                                                                tGTACGGCGATGCGGGCGTGGCAATTGAGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGAgg     <  1:2555262/69‑1 (MQ=255)

                                                                    cGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGAGGTAt  <  1:3166493/68‑1 (MQ=255)

                                                                    cGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGAGGTAt  <  1:2908020/68‑1 (MQ=255)

TTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGAGGTAT  >  minE/1781658‑1781793

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A